• Plant Resources
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• 제8권1호
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• pp.60-66
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• 2005

Lysine is the first essential amino acid for optimal nutrient quality in rice grain. For the narrow genetic diversities of lysine contents in rice, somaclonal variation was the source of mutation in our breeding program. Biochemical selection was conducted using 1 mM S-(2-aminoethyl) cysteine followed by two passages of 5 mM lysine plus threonine in the callus subculture medium. The lysine contents in endosperm of all progenies recovered from the biochemical selection were higher than those of their donor cultivar 'Hwayeongbyeo'. These elevated lysine levels of mutants were successfully transmitted to $M_4$ generation. The lysine contents in endosperm varied 3.85 to $4.80\%$ compare to their donor cultivar 'Hwayeongbyeo' was $3.85\%$. Three of high-lysine germplasms, Lys-l, Lys-2 and Lys-7 were selected by biochemical selection and rapid screening methods. DNA analysis showed that a new insertion of Tos 17 which mapped to rice chromosome 11 on the high-lysine mutant, Lys-2.

• 한국습지학회지
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• 제8권3호
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• pp.39-49
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• 2006

Seasonal biomass and carbon, nitrogen contents change of marsh club-rush (Schoenoplectus trigueter) was investigated in Nakdong river estuary, located near Busan, Korea. New shoot of S. trigueter sprouted from tuber in April and fast growth season was followed until mature in August. Mature lengths of shoot and root were 60 and 9.4 cm, respectively. The increase of biomass showed similar seasonal trends with length. Mature biomass were $3.5gind^{-1}$ in wet weight and $0.6gind^{-1}$ in dry weight. The biomass of S. trigueter in areal basis was also highest during July and August ($186gDWm^{-2}$). The shoot of S. trigueter was disappeared in October from the ground but the biomass of shoot was maintained as a form of detritus in sediment. The amount of S. trigueter detritus was about 30~50% of the biomass in August. During winter, the amount of detritus decreased with time but the biomass of root+tuber remained same, implying the root+tuber part is alive. The net productivity of S. trigueter estimated from biomass change were $538gDWm^{-2}yr^{-1}$, $240g-Cm^{-2}yr^{-1}$, $8.2g-Nm^{-2}yr^{-1}$ in dry weight, carbon and nitrogen equivalent respectively. During winter, carbon to nitrogen ratio in detritus increased implying the preferred remineralization of nitrogen during microbial degradation.

• Journal of Nutrition and Health
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• 제27권2호
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• pp.127-140
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• 1994

The effect of milk in low and high cholesterol diet was invesigated on serum cholesterol metabolism and lipid contents of serum, aorta, liver of rats. Weanling male Sprague-Dawley rats were divided into low(0.01% w/w) and high(1.01% w/w) cholesterol-diets groups. Low cholesterol groups subdivided into four groups ; control group was given water and three milk groups were given low heat milk(LM), ultra-high heat milk(HM), and powder milk(PM), respectivily, instead of water. High cholesterol groups were consisted of three groups ; control, LM, and HM groups. After feeding these experimental diets for six weeks, lipid levels were measured in serum and tissure and dried feces were analyzed for neutral and acidic sterols. Results obtained from this study are as follows : 1) Nutrient intakes, body weight gains and aorta weights did not differ among groups, but liver weights were higher in high cholesterol fed rats than low cholesterol fed rats. 2) Serum protein contents were increased independently by intakes of high cholesterol and milk. 3) Serum total cholesterol and triglyceride levels were increased but phospholipid levels and HDL-cholesterol/total-cholesterol ratios were decreased by high cholesterol in diet. And milk supplementation decreased serum total cholesterol and triglyceride levels but increased phospholipid levels and HDL-cholesterol/total-cholesterol ratios. 4) Contents of cholesterol and triglyceride in aorta and liver were elevated by dietary high cholesterol and lowered by consumption of all three types of milk. 5) Levels of cholesterol and triglyceride among serum, aorta and liver were highly correlated (r=0.7-0.9, p<0.001). 6) Fecal excretion of total sterols was three times high in high cholesterol group, compared with low cholesterol groups and were increased about 20% by milk consumption. 7) The effects of milk were more pronouncely shown in low cholesterol groups and mostly confined to LM and HM groups, rarely shown in PM group. It is concluded from the present study that milk had the hypolipidemic as well as hypocholes terolemic effect, which appears to be mediated through increased fecal bile acid excretion. But the effect is likely to be shaded by excess consumption of dietary cholesterol and was almost absent in powder milk.

• Journal of Animal Science and Technology
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• 제61권5호
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• pp.278-284
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• 2019

This study was carried out to investigate the effects of seeding dates on the yield and feed value of whole-crop barley cultivated in paddy soil. The field experiment was conceived as a randomized block design performed in triplicate with seeding dates of Oct. 19 (T1 as treatment 1), Oct. 26 (T2 as treatment 2), Nov. 2 (T3 as treatment 3), Nov. 9 (T4 as treatment 4), and Nov. 16 (T5 as treatment 5) as treatments. The barley grown in all treatments was harvested on May 21 of the following year. Plant length, fresh yield, dry matter yield, and total digestible nutrient (TDN) yield were higher (p < 0.05) in barley with early seeding dates, whereas crude protein and ether extract were higher (p < 0.05) with late seeding dates. Crude fiber and neutral detergent fiber were highest in the T3 treatment and lowest in the T1 treatment. There was no significant difference between acid detergent fiber and TDN among the different seeding dates. Total mineral contents were higher in the order T1 > T2 > T3 > T4 > T5 (p < 0.05), whereas the total amino acid content increased significantly (p < 0.05) with a delay in seeding date. Free sugar contents (fructose, glucose, and sucrose) were higher with early seeding dates (p < 0.05). Collectively, the results obtained in this study indicate that it is favorable to seed soon after harvesting rice to increase dry matter and TDN yields and mineral and free sugar contents of whole-crop barley in the midlands of Korea.

• 한국식품저장유통학회지
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• 제20권3호
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• pp.336-341
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• 2013

국내에서 주로 재배되는 무화과 품종인 도후인(Dauphine) 2종, 봉래시(Horaish) 2종, 바나네(Banane) 1종을 대상으로 이화학적 특성을 분석한 결과, 무화과의 수분은 85.37~87.28%, 회분 0.38~0.43%, 조지방 0.20~0.26%, 조단백질 0.39~0.81%로 품종별 차이는 보이지 않았으며 조섬유는 바나네가 $7.55{\pm}0.13%$로 다른 품종 5.12~7.60%에 비해 다소 높은 결과를 보였다. 무기성분 함량은 모든 품종에서 K>Ca>Mg>Na 순으로 나타났으며 전체 함량의 경우 도후인 품종이 타 품종에 비해 조금 높은 결과를 보였다. 바나네 품종과 봉래시 품종의 총 유리아미노산 조성은 glutamic acid>proline>aspartic acid 순으로 높은 분포를 보였으며, 전체 함량의 51.68~58.71%를 차지하고 있으며, 도후인 품종의 경우 glutamic acid>aspartic acid>alanine이 전체의 약 50%를 차지하고 있었다. 또한 기억력 감퇴 예방 등 학습 능력에 도움을 주는 기능성 물질인 ${\gamma}$-aminobutyric acid(GABA) 함량은 바나네에서 26.69 mg/100 g, 도후인이 13.57~18.38 mg/100 g, 봉래시가 16.53~16.93 mg/100 g으로 상당히 높은 함량을 보였다. 총 폴리페놀 함량은 도후인이 198.91~261.64 mg/kg, 봉래시 품종이 169.90~174.33 mg/kg, 바나네 품종이 211.07 mg/kg 으로 나타났으며, 총 플라보노이드 함량은 바나네(84.95 mg/kg), 도후인(60.47~68.71 mg/kg), 봉래시(44.12~44.60 mg/kg) 순으로 바나네가 가장 높았으며 품종간의 차이를 보였다. 플라보노이드 성분 중 quercetin과 kaempferol은 각각 2.40~3.54 mg/kg, 1.09~1.16 mg/kg으로 분석되었다.

• 한국습지학회지
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• 제14권1호
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• pp.121-130
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• 2012

본 연구에서는 2011년 6월부터 11월까지 충청남도 논산시 양지리의 가축폐수 처리를 위해 조성된 인공습지에서 정수식물인 갈대의 처리공정별 계절별 생물량 변이와 질소 인의 흡수량, 수처리 효율을 파악하기 위하여 수행되었다. 조사기간 동안 인공습지 내 갈대의 총 생물량은 200.1 ~ 669.4 kg의 범위로 보이며 계절별 총 생물량은 7월에 669.4 kg로 가장 높게 나타났다. 인공습지 내 갈대의 지상부 질소 함량은 $21.9{\pm}0.6{\sim}32.1{\pm}1.5mg/g$, 지하부는 $15.1{\pm}5.5{\sim}24.9{\pm}5.7mg/g$, 인의 함량은 지상부에서 $1.5{\pm}0.3{\sim}2.4{\pm}0.2mg/g$, 지하부는 $1.6{\pm}0.6{\sim}2.5{\pm}0.6mg/g$의 범위로 조사되었다. 인공습지 내 갈대의 질소 흡수량은 9.7 ~ 28.0 kg, 인의 흡수량은 0.7 ~ 2.8 kg의 범위로 조사되었다. 질소의 흡수량은 7월에 28.0 kg으로 가장 높게 나타났으며 11월에 9.7 kg으로 가장 낮게 나타났다. 인의 흡수량은 6월에 2.8 kg으로 가장 높게 나타났으며 11월에 0.7 kg으로 가장 낮게 나타났다. 인공습지로 이입되는 가축폐수의 질소 인 저감 효율은 각각 23.0, 59.1 %로 조사되었다. 본 연구결과는 향후 인공습지 내 오염물질 및 영양염류 순환 도출에 기여할 것으로 판단된다.

• 생물환경조절학회지
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• 제17권3호
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• pp.238-245
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• 2008

마그네슘 시비농도를 인위적으로 조절하여 '매향' 딸기를 관비재배하면서 Mg의 시비수준이 생장과 결핍증상 발현에 미치는 영향을 구명하고, 건전생육을 유지할 수 있는 식물체 및 토양의 한계농도를 밝히기 위하여 본 연구를 수행하였다. 마그네슘 결핍증상은 하위엽에서 발생하였으며, 초기에 하위엽의 엽맥 사이에서 반점 형태의 황화현상이 나타난 후 점차 반점 부위가 확산되어 엽맥간 황화현상으로 발전하였다 또한 증상이 심해지면서 엽맥 사이가 검게 변하고, 하위엽 선단의 갈변 및 괴사하는 증상이 발생하였다. Mg시비농도를 조절하여 관비하고 정식 120일 후에 지상부 생육을 조사한 결과 Mg 1.0 또는 2mM의 처리에서 생육이 우수하였으며, 0.5 이하나 4mM 이상으로 Mg 시비농도를 조절한 처리의 생장이 저조해지는 경향이었다. 건물중은 마그네슘 시비농도에 대하여 3차 곡선회귀적인 반응을 보였으며 식물체당 약 8.2g의 건물중을 생산할 때 정점이 형성되었다. 최대 생장량의 90%를 최저 한계점으로 간주하면 식물체당 7.4g 이상의 건물중을 생산하기 위해서는 Mg 함량이 $0.30\sim0.65%$의 범위에 포함되도록 시비해야 하며, 최적 시비농도는 약 2mM 이라고 판단하였다. 또한, 최대 생산량인 식물체당 생체중 36.2g의 90%를 최저 및 치고 한계점으로 간주 할 경우 엽병 추출액의 Mg농도가 $19\sim40mg{\cdot}kg^{-1}$의 범위에 포함되도록 시비해야 한다고 판단하였다.

• 한국토양비료학회지
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• 제25권4호
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• pp.334-341
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• 1992

석천미사질토양(石泉微砂質土壤)의 답전륜환포장(畓田輪換圃場)에서 수도(水稻)의 생산력변화와 식물체중의 무기성분흡수양상(無機成分吸收樣相)의 특징(特徵)등에 대해서 수도연작구(水稻連作區)와 수환구(輸換區)와를 비교검토(比較檢討)한 결과(結果)를 요약(要約)하면 다음과 같다. 1. 정조수량(精租收量)은 '90년(年)(2년차(年次)) 및 '91년(年)(3년차(年次))에서 벼연작구(連作區)에 비하여 매년윤환구(每年輪換區) 및 2년윤환구(年輪煥區)의 감자, 배추구(區)>대두구(大豆區)>대두구(大豆區))>벼연작구(連作區)의 순(順)이며 증수요인(增收要因)은 주당수수(株當穗數), 수당립수(穗當粒數)의 증가(增加)와 등숙률(登熟率)의 향상(向上)에 기인(基因)되었다. 2. 2년간(年間)('90~'91년(年))의 수확기(收穫期) 무기성분함량(無機成分含量) 및 흡수량(吸收量)은 수량(收量)의 증수경향(增收傾向)과 같은 양상(樣相)으로 증가(增加)되었으며 특(特)히 3년차(年次)('91년(年))의 무기성분흡수량(無機成分吸收量)이 질소시비수준(窒素施肥水準)에 따라 증대(增大)되는 경향(傾向)이었다. 3. 2년차(年次)('90년(年)) 수도흡수질소(水稻吸收窒素)의 토양유내(土壤由來)의 질소(窒素)는 수도연작구(水稻連作區)<매년윤환대두구(每年輪換大豆區)<매년윤환(每年輪換)감자, 배추구(區)의 순(順)이었다. 4. 군락상태하(君落狀態下)에서 수도엽색(水稻葉色)의 경시적(經時的) 변화(變化)는 토양용액중(土壤溶液中)의 암모니아태질소함량(態窒素含量)에 따라 변화(變化)되는 양상(樣相)이 큰 경향(傾向)이다.

• Journal of Nutrition and Health
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• 제34권7호
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• pp.797-808
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• 2001

To determine folate intake and food sources in Korea college students, dietary survey was conducted in March, 1999. Dietary data were collected by trained interviewers using the method of 24-hour recalls for 3 consecutive days. The data of 44 male and 62 female students were analyzed with two different nutrient databases in Recommended Dietary Allowances for Korean on the 6th and 7th revisions, and the results were compared. The intakes of energy and nutrients except vitamin A and folate were lower when analyzed with the 7th database than the 6th database. Mean daily folate intakes with the 6th and 7th databases were 172.9$\mu\textrm{g}$, 221.6$\mu\textrm{g}$ for male students while 125.1$\mu\textrm{g}$, 168.0$\mu\textrm{g}$ for female students, respectively. The results showed significantly higher estimates of folate intake with the 7th database, and significantly higher intake in males than females analyzed with both the 6th and 7th databases. Daily folate intake per 1,000kcal (folate density) was 71.1$\mu\textrm{g}$/1000kcal for males and 67.6$\mu\textrm{g}$/1000kcal for females with the 6th database, and 97.9$\mu\textrm{g}$/1000kcal for males and 95.5$\mu\textrm{g}$/1000kcal for females with the 7th database. The differences in folate density between the two databases were signficant, but the differences between the gender were not significant. The proportions of the subjects who consumed more than the RDA of 250$\mu\textrm{g}$ with the 6th and 7th databases were only 4.6%, 29.6% of males and 1.6%, 9.7% of females, respectively. The biggest food source of folate was Kimchi, contributing 17.9% for male and 13.7% for female students with 7th database. Laver, spinach, Ramyon, rice, and Ko Chu Jang together with Kimchi contributed 41.9% for male and 32.4% for females students with the 7th database. These results imply that folate intake reported in the reported inthe previous studies using the 6th database was underestimated. However, the 7th database seems to be still incomplete since 20.5% of 2,932 foods in the database were derived from the other sources, and the rest were imputed from similar foods. Therefore, in order to accurately estimate folate intake of Koreans, folate contents in major contributing foods need to be measured using an appropriate assay method.

• Journal of Nutrition and Health
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• 제39권5호
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• pp.485-493
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• 2006

The purpose of this study was to investigate the relationships among nutrient intakes, BMD, serum copper, zinc and manganese contents in Korean postmenopausal women residing in Seoul and Kyunggi-do. Nutrient intakes analyses and anthropometric measurements were performed for 64 postmenopausal women. Also, fasting blood samples were collected. The BMDs of the lumbar spine (L2-L4) and femoral neck were measured by dual energy X-ray absorptiometry (DEXA). Subjects were classified into three groups: osteoporosis (T-score ${\leq}$ -2.5, n = 20), osteopenia (-2.5 < T-score ${\leq}$ -1, n = 24), and normal (T-score) -1, n = 20). The results are summarized as follows; the mean age, height and weight were 62.1 yrs, 153.8 em, and 56.1 kg, respectively, with no significant difference among three groups. On the other hand, the mean BMI $(23.7\;g/m^2)$ of normal group was significantly higher than those of other two groups. The mean energy intake was 1,395.3 kcal (77.5% of Dietary referance intakes for koreans (KDRls)). The average daily intakes of vitamin $B_2$, calcium and zinc did not reach KDRIs. The mean intakes of iron, copper, zinc and manganese were 10.9 mg, 1.6 mg, 7.8 mg and 5.1 mg respectively, with no significant difference among three groups, The mean serum levels of copper, zinc and manganese were 96.4 mg/dl, 91.5 mg/dl and 39.5 ng/dl, respectively with no significant differences among three groups. The BMD of lumbar spine showed a significantly negative correlation intakes of animal protein, fat and iron intakes after adjusting for age, Bill. However, the BMD of lumbar spine was not significantly correlated with animal iron intakes after adjusting for animal protein, and fat intakes, indicating animal food intake is a possible confounding factor. Proper intakes of vitamin $B_2$, vitamin C are required to protect osteoporosis in postmenopausal women. These results suggest that excessive animal food intakes may have adverse effect on bone mineral density. Further studies arc necessary to elucidate the role of serum minerals in BMD.