• Research in Plant Disease
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• v.30 no.2
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• pp.148-156
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• 2024

Ralstonia pseudosolanacearum, a plant pathogenic bacterium that can survive for a long time in soil and water, causes lethal wilt in the Solanaceae family. Sigma S is a part of the RNA polymerase complex, which regulates gene expression during bacterial stress response or stationary phase. In this study, we investigated the role of sigma S in R. pseudosolanacearum under stress conditions using a rpoS-defective mutant strain of R. pseudosolanacearum and its wild-type strain. The phenotypes of rpoS-defective mutant were complemented by introducing the original rpoS gene. There were no differences observed in bacterial growth rate and exopolysaccharide production between the wild-type strain and the rpoS mutant. However, the wild-type strain responded more sensitively to nutrient deficiency compared to the mutant strain. Under the nutrient deficiency, the rpoS mutant maintained a high bacterial viability for a longer period, while the viability of the wild-type strain declined rapidly. Furthermore, a significant difference in pH was observed between the culture supernatant of the wild-type strain and the mutant strain. The pH of the culture supernatant for the wild-type strain decreased rapidly during bacterial growth, leading to medium acidification. The rapid decline in the wild-type strain's viability may be associated with medium acidification and bacterial sensitivity to acidity during transition to the stationary phase. Interestingly, the rpoS mutant strain cannot utilize acetic acid, D-alanine, D-trehalose, and L-histidine. These results suggest that sigma S of R. pseudosolanacearum regulates the production or utilization of organic acids and controls cell death during stationary phase under nutrient deficiency.

• Nutrition Research and Practice
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• v.9 no.1
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• pp.57-62
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• 2015

BACKGROUND/OBJECTIVES: The prevalence of atopic dermatitis in infants is increasing worldwide. However, the nutrient intake status of infants with atopic dermatitis has not been studied properly. This study was conducted to compare the nutrient intake status of infants in the weaning period with atopic dermatitis by feeding type. MATERIALS/METHODS: Feeding types, nutrient intake status and growth status of 98 infants with atopic dermatitis from age 6 to 12 months were investigated. Feeding types were surveyed using questionnaires, and daily intakes were recorded by mothers using the 24-hour recall method. Growth and iron status were also measured. RESULTS: The result showed that breastfed infants consumed less energy and 13 nutrients compared to formula-fed or mixed-fed infants (P < 0.001). The breastfed group showed a significantly lower intake rate to the Dietary Reference Intakes for Koreans than the other two groups (P < 0.001). In addition, they consumed less than 75% of the recommended intakes in all nutrients, except for protein and vitamin A, and in particular, iron intake was very low, showing just 18.7% of the recommended intake. There was no significant difference in growth by feeding type, but breastfed infants showed a significantly higher rate of iron deficiency anemia (P < 0.001). CONCLUSIONS: Continuous management programs should be prepared for breastfed infants with atopic dermatitis, who are in a period when rapid growth takes place and proper nutrient intake is essential.

• Preventive Nutrition and Food Science
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• v.9 no.4
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• pp.383-388
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• 2004

This study was designed to identify how the incidence and severity of premenstrual. syndrome (PMS) correlate with the nutrient intakes and exercise habit of women. The subjects of this study were 299 women residing in Busan metropolitan city. Each subject was asked to complete a menstrual discomfort questionnaire (MDQ) for PMS and nutrient intakes. PMS symptom scores of women in their twenties ranked in order of severity were: behavioral change (2.45), followed by pain (2.36) and water retention (2.28), negative effects (2.20), autonomic reaction (1.91), arousal (1.87), decreased concentration (1.76) and decreased control (1.74). For Women in their thirties, the symptom of pain was the most dominant (2.93) followed by autonomic reaction (2.69) and behavioral change (2.54), and for those in their forties, negative effect (3.06) was highest, followed by pain (2.97) and autonomic reaction (2.86). The overall symptoms of PMS significantly increased with age (20': 2.07 points, 30': 2.34 points, 40': 2.47 points). There was no correlation of the BMI of the subjects with the symptoms of PMS, but there was a significant negative correlation between the symptoms of PMS and exercise frequency for women in their thirties and forties. Subjects in their twenties exhibited a significant negative correlation for PMS symptoms with the intake of carbohydrate (p < 0.05), calcium (p < 0.05) and vitamin E (p < 0.05). For subjects in their thirties, PMS symptoms were negatively correlated with the intake of calcium (p < 0.05) and vitamin C (p < 0.05); and in women in their forties, calcium (p < 0.01) and carbohydrate (p < 0.05) intakes were negatively correlated with PMS symptoms. This suggests that PMS represents the clinical manifestation of a nutrient deficiency state, especially calcium. Therefore, we concluded that nutrient supplementation and exercise management are likely to be of benefit in relieving PMS symptoms.

• Proceedings of the Korean Environmental Sciences Society Conference
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• 2003.05a
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• pp.261-262
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• 2003

Sunflower(Helianthus annuus L.) seedings were de-rooted and grown in nutrient solutions providing either deficient or sufficient boron supply and supplemented with aluminum. Increasing concentrations of aluminum in the nutrient medium caused progressive inhibition of root growth and a parallel increase in proline level of roots. Elevated boron levels improved root growth under toxic aluminum conditions and produced higher proline contents. Exogenous ascorbate improved adventitions root growth in plants supplied with insufficient boron and aluminum. These findings suggest that root growth inhibition resulting from either boron deficiency or aluminum toxicity may be a result of impaired ascorbate metabolism.

• Proceedings of the Korean Society of Crop Science Conference
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• 2023.04a
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• pp.103-103
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• 2023

• Korean Journal of Community Nutrition
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• v.8 no.3
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• pp.302-310
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• 2003

This study was undertaken to assess nutritional status of the low income elderly residing in Gwangju. Anthropometric data showed that mean height of the subjects was lower than that of Korean Standard Growth data but weight was similar to that standard value. As the subjects became older, their heights and weights were decreased. Body mass index (BMI) in males and females were 22.9 and 24.4 respectively. However, BMI distribution showed that 56% of the elderly females under 75 were underweight. Advancing age conoibutes significant difference in triceps skinfold thickness in females (p < 0.001). Total cholesterol level was higher in the elderly females than the males. It was found that a considerable number of subjects had anemia determined by hemoglobin and hematocrit level, which indicated un iron deficiency. Twenty-four hour dietary recall revealed that, except for phosphorus and vitamin C, all the nutrient intakes of the subjects were below 75％ of Korean RDA. Surprisingly, vitamin A and riboflavin intakes of the elderly were below 50％ of Korean RDA. Energy intakes of the elderly males and females were 58.7% and 59.6% of Korean RDA respectively. Unbalanced energy ratios of carbohydrate, protein and fat were noted in both genders. Nutrient intakes of females' were lower than those of males'. Thus, there was a significant gender difference in nutrient intakes. It might be further suggested that an appropriate nutritional program should be developed and implemented to improve the Poor nutritional status of the low income elderly living in cities.

• Journal of Community Nutrition
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• v.6 no.2
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• pp.67-77
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• 2004

In order to investigate the differences of nutrient intakes by the economic status and different age groups and to identify the nutritional risk group and its specific nutrition problem, 2001 Korean National Health and Nutrition Survey were analyzed. The subject's numbers of 9,391 were classified into four classes such as low (14.2%), medium (37.2%), high (26.0%), and high above (22.6%) on the basis of the family monthly income and the 2001 Korean minimum cost of living according to the family size. Mean intakes of energy and all nutrients assessed by the RDAs, lipid-energy %, and MAR were increased as the economic status were going up. Na intake expressed per 1,000kcal was in reverse. Nearly a half(45.5%) of the low-income people seemed to take nutritionally inadequate diet in consideration with MAR values. Deficiencies of iron and even energy in the toddlers (1 to 2 years) of low-income class were of great concern. Adolescent age group has been observed that their calcium and iron intakes, and possibly energy, were appeared to be the most deficient among all the age groups regardless of the economic status. For the elderly in all the economic status except high-above class, calcium, vitamin A, and riboflavin were commonly deficient nutrients. Calcium deficiency was appeared throughout nearly all the ages except toddlers and all the economic classes. Even in the high-above class 57.3% took insufficient amount of calcium.

• Journal of Digestive Cancer Research
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• v.1 no.2
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• pp.82-88
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• 2013

Cancer is the first leading cause of death in Korea and the second leading cause of death in the USA. There is extensive research into prevention of cancer and the support of oncology patients with diet or dietary supplements. In vitro and in vivo animal studies have indicated that antioxidants, including beta-carotene, alpha-tocopherol, and ascorbic acid, can yield anti-cancer effects in addition to providing protection against oxidative damage. Although many observational studies have shown that consuming fruits and vegetables can reduce the risk of some cancers, the results of several large-scale human intervention trials testing the benefits of a single or combined higher-dose of individual micronutrients have been inconsistent. Cancer can cause profound metabolic and physiological changes which may affect patients' nutrient requirements. Although the optimal route of nutrient delivery is through diet, cancer patients often suffer symptoms that disrupt their food intake, including anorexia, premature satiety, altered taste and smell, and changes in bowel mobility. In particular, micronutrient deficits can slow postoperative healing, contribute to depression symptoms, and decrease immune competence. Cancer patients are generally motivated to take dietary supplements to improve responses to treatment and quality of life. The Physician's Health Study II (PHS II) randomized controlled trial reported recently that daily multivitamin supplementation significantly, albeit modestly, reduced the risk of total cancer. Although evidence of multivitamin use benefits is limited in cancer patients, taking dietary supplements with constituents in the range of the recommended daily allowance according to the Dietary Reference Intake (DRI) recommendation is generally considered to be safe.

• Microbiology and Biotechnology Letters
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• v.20 no.4
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• pp.363-370
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• 1992

Microorganisms capable of accumulating poly-p-hydroxybutyric acid(PHB) were isolated from soil by enrichment culture technique. Among them, the strain designated as FL-027 had high PHB productivity and was identified as Alcaligenes. The optimal medium composition for cell growth was 8.0 $g/\ell$ of fructose and 3.0 $g/\ell$ of $(NH_4)_2S0_4$, equivalent to C/N ratio 5.04 at pH 7.0 and $30^{\circ}C$. To investigate the optimal conditions for the PHB accumulation, we divided the process into two stages; the first stage for the growth of the cell in nutrient-rich medium and the second stage for the PHB accumulation in nutrient-deficiency medium. The optimal conditions for PHB accumulation were 8.0 $g/\ell$ of fructose and 0.25 $g/\ell$ of $(NH_4)_2S0_4$, equivalent to C/N ratio 60 at pH 6.5 and $30^{\circ}C$. PHB accumulation was stimulated by deficiency of nutrients such as $NH_4^+$, $Ca^{2+}$, $SO_4^{2+}$ in medium. Among them. $NH_4^+$ deficiency was chosen because of its effectiveness. We found the inhibition of cell growth by fructose in batch culture. In order to keep the fructose concentration at an optimal level, intermittent feeding fed-batch culture was employed, and the cell concentration as high as 10.83 $g/\ell$ whose PHB content was responsible for 43% of the dry cell weight. The purified PHB was identified as homopolymer of 3-hydroxybutyric acid by using IR and $^1H-NMR$.

• Journal of Nutrition and Health
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• v.32 no.8
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• pp.887-896
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• 1999

The purpose of this research is to assess hematological and biochemical status and the prevalence of iron deficiency of pregnant women by gestational age to provide the primary data about iron nutritional status of pregnant women. Pregnant women visiting public health centers in Ulsan participated in study and were divided into 3 trimester by last menstrual period(LMP). Hemoglobin (Hgb), hematocrit(Hct)and mean corpuscular volume(MCV) among iron status indices were not statistically different from normal distribution, however total iron binding capacity(TIBC) and serum ferritin were skewed to left and serum iron and transferrin saturation(TS) were skewed to right. Hgb was positively correlated with Hct(r=0.93, p<0.001) but TIBC was negatively correlated with all indices. Serum ferritin was also correlated with all indices, especially in 3rd trimester but not reached to 1st trimester level. Mean corpuscular hemoglobin(MCH), mean corpuscular hemoglobin concentration(MCHC), Red cell distribution width(RDW), serum iron and TS were not significantly different by trimester, however when serum serum iron was adjusted with hematocrit to correct the hemodilution, it significantly decreased in 2nd trimester. MCV increased in 2nd trimester and was maintained until late pregnancy, TIBC continued to increase throughout the trimester. The prevalence of anemic by CDC(Centers for Disease Control) Hgb criteria(Hgb <11.0g/dl in 1st and 3nd trimester, Hgb<10.5g/dl in 2nd trimester) was 2.8% in 1st trimester, 22.5% in 2nd trimester, 27.1% in 3rd trimester and was similar with prevalence by CDC Hct criteria(Hct < 33% in 1st and 3rd, Hct < 32% in 2nd). The prevalence of anemic of total subjects was 32.7% by WHO criteria(Hgb < 11.0g/dl). Although almost iron status indices increased in 3rd trimester, the prevalence of anemia by different criteria of all indices increased throughout the trimester, so iron nutritional status was considered as serious during late pregnancy. However, since factors other than iron deficiency, such as infection, infection, inflammation, other nutrient deficiency may also play a significant role, to differentiate the anemia due to mainly iron deficiency from the anemia due to other factors, serum ferritin is among the more useful indices in distinguishing the two conditions because it is depressed only in iron deficiency. Hgb<11.0g/dl and serum ferritin<12.0ug/L as the criteria of iron deficiency was suggested by CDC. 17.8% of all subjects were classified as iron deficient anemia, 14.9% as anemic from other reasons, 21.2% as iron deficiency any only 46.2% were in normal iron status.