• Korean Journal of Clinical Laboratory Science
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• v.54 no.4
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• pp.298-306
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• 2022

This study sought to evaluate the mechanism of cellular injury caused by ferrous sulfate (FeSO₄) and the protective effects of Parnassia palustris L. (PP) extract against FeSO₄-induced cytotoxicity of cultured C6 glioma cells. FeSO₄ is known to cause neurotoxicity and induce Parkinson's disease. The antioxidative effects of PP, such as superoxide dismutase (SOD)-like and superoxide anion-radical (SAR)-scavenging activities, as well as effects on cell viability, were studied. FeSO₄ significantly decreased cell viability in a dose-dependent manner and the XTT₅₀ value, the concentration of FeSO₄ which reduced the cell viability by half, was measured at 63.3 μM in these cultures. FeSO₄ was estimated to be highly cytotoxic by the Borenfreund and Puerner toxicity criteria. Quercetin, an antioxidant, significantly improved cell viability, damaged by FeSO₄-induced cytotoxicity. While evaluating the protective effects of the PP extract on FeSO₄-induced cytotoxicity, it was observed that the extract significantly increased cell viability compared to the FeSO₄-treated group. Also, the PP extract showed superoxide dismutase (SOD)-like and superoxide anion radical (SAR)-scavenging activities. Based on these findings, it can be concluded that FeSO₄ induced oxidative stress-related cytotoxicity, and the PP extract effectively protected against this cytotoxicity via its antioxidative effects. In conclusion, natural antioxidant sources such as PP may be agents useful for preventing oxidative stress-related cytotoxicity induced by heavy metal compounds such as the FeSO₄, a known Parkinsonism inducer.

• Journal of Internet of Things and Convergence
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• v.9 no.1
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• pp.33-39
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• 2023

As the number of people with chronic diseases increases due to an aging society, it is urgent to prevent and manage their diseases. Although biometric authentication methods and Telemedicine Systems have been introduced to solve these problems, it is difficult to solve the security problem of medical information and personal authentication. Since smart healthcare includes personal medical information of subjects, the security of personal information is the most important field. Therefore, in this paper, we tried to propose a Telemedicine System using a smart wearable device ECG in the form of a wristband and face personal authentication in a private blockchain environment. This system targets various medical personnel and patients with chronic diseases in all regions, and uses a private blockchain that can increase data integrity and transparency, ECG and face authentication that are difficult to forge and alter and have high personal identification to provide a system with high security and reliability. composed. Through this, it is intended to contribute to increasing the efficiency of chronic disease management by focusing on disease prevention and health management for patients with chronic diseases at home.

• Journal of Chest Surgery
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• v.56 no.3
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• pp.155-161
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• 2023

Background: Surgical closure of an atrial septal defect (ASD) is infrequently indicated during infancy. We evaluated the clinical characteristics and outcomes of patients who underwent surgical ASD closure during infancy. Methods: A single-center retrospective review was performed for 39 patients (19 males) who underwent surgical ASD closure during infancy between 1993 and 2020. The median body weight percentile at the time of operation was 9.3. Results: During a median follow-up of 60.9 months, 4 late deaths occurred due to chronic respiratory failure. A preoperative history of bronchopulmonary dysplasia (BPD) was the only risk factor for late mortality identified in Cox regression (hazard ratio, 3.54; 95% confidence interval [CI], 1.75-163.04; p=0.015). The 5-year survival rate was significantly lower in patients with preoperative history of BPD (97.0% vs. 50.0%, p<0.001) and preoperative ventilatory support (97.1% vs. 40.4%, p<0.001). There were significant postoperative increases in left ventricular end-diastolic (p=0.017), end-systolic (p=0.014), and stroke volume (p=0.013) indices. A generalized estimated equation model showed significantly better postoperative improvement in body weight percentiles in patients with lower weight percentiles at the time of operation (<10th percentile, p=0.01) and larger indexed ASD diameter (≥45 mm/m², p=0.025). Conclusion: Patients with ASD necessitating surgical closure during infancy are extremely small preoperatively and remain small even after surgical closure. However, postoperative somatic growth was more prominent in smaller patients with larger defects, which may be attributable to an increase in postoperative cardiac output due to changes in ventricular septal configuration. The benefits of ASD closure in patients with BPD are undetermined.

• Journal of Life Science
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• v.33 no.5
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• pp.397-405
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• 2023

Although glutathione (GSH) has been shown to play an important role in the prevention of oxidative damage as an antioxidant, studies on immune regulation by it have not been properly conducted. In this study, we investigated whether luthione^®, a reduced GSH, has an immune enhancing effect in murine macrophage RAW 264.7 cells. The results of flow cytometry and immunofluorescence experiments indicated that luthione increased phagocytic activity, a representative function of macrophages, compared to the control cells. According to the results of the cytokine array, the expression of interleukin (IL)-5, IL-1β, and IL-27 was significantly increased in the luthione-treated cells. Luthione also enhanced the production of tumor necrosis factor-α and IL-1β through increased expression of their proteins, and increased release of the immune mediators such as nitric oxide (NO) and prostaglandin E₂ was associated with increased expression of inducible NO synthase and cyclooxygenase-2. In addition, the expression of cluster of differentiation 86, an M1 macrophage marker, was dramatically enhanced in RAW 264.7 cells treated with luthione. Furthermore, as a result of heat map analysis, we found that cytokine signaling 1/3-mediated signal transducer and activator of transcription/Janus tyrosine kinase signaling pathway was involved in the immunomodulatory effect by luthione. In conclusion, our data suggested that luthione could act as a molecular regulator in M1 macrophage polarization and enhance immune capacity by promoting macrophage phagocytic function.

• Journal of the Korean Applied Science and Technology
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• v.40 no.3
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• pp.392-401
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• 2023

This study was to identify the association between osteoporosis knowledge and performance of osteoporosis preventive behaviors among low-income middle-aged women, and to determine the impact of these variables on osteoporosis preventive behaviors. The subjects were 317 middle-aged women from low-income who visited three general hospitals in P city, and data were collected from February 1, 2021 to December 30, 2022 using structured questionnaires. The collected data were analyzed by descriptive statistics, t-test, ANOVA, Pearson's correlation, and Stepwise multiple linear regression using SPSS/WIN 27.0 program. As a result of the study, the osteoporosis knowledge of the subjects was low at 11.97±6.89 points, which showed a difference in the case of osteoporosis prevention education, fracture experience. The osteoporosis prevention behavior was low at 41.03±7.63 points, which showed a difference according to religion, comorbidity, osteoporosis prevention education and fracture experience, and subjective health status. There was a statistically positive correlation between osteoporosis knowledge and osteoporosis prevention behavior(r=.527, p<.001), and the factors affecting osteoporosis prevention behavior were fracture experience(𝛽=.415, p<.001), osteoporosis prevention education(𝛽=.359, p<.001), and self-rated health(𝛽=.186, p<.001). Therefore, it is necessary to develop a program that can promote osteoporosis prevention behavior of middle-aged women in low-income and to prepare measures to encourage participation.

• Journal of the Korean Applied Science and Technology
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• v.40 no.3
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• pp.462-475
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• 2023

This study is descriptive research to identify the effect of COVID-19 stress on premenstrual syndrome in female college students and the mediating effect of depression and coping with premenstrual syndrome. The data were collected through an on-line survey for 20 days from November 11 to November 30, 2021, targeting female college students attending C College located in S city. As a result of analyzing a total of 161 data, COVID-19 stress was positively correlated with premenstrual syndrome(r=.383, p<.001) and depression(r=.436, p<.001), and coping with premenstrual syndrome had a positive correlation only in premenstrual syndrome(r=.190, p=.016). As a result of verifying the mediating effect of depression and coping with premenstrual syndrome between COVID-19 stress and premenstrual syndrome, COVID-19 stress had a significant direct effect on premenstrual syndrome(β=4.907, p=.002), and depression(β=6.780, p<.001), and depression had a significant direct effect on premenstrual syndrome(β=.383, p=<.001), coping with premenstrual syndrome had a direct effect on premenstrual syndrome(β=4.292, p=.030) respectively. The indirect effect of verifying the mediating effect in the relationship between COVID-19 stress and premenstrual syndrome confirmed that there was a statistically significant effect only in the path via depression(95% CI, 1.282 to 4.140). Therefore, it is necessary to develop an intervention strategy to reduce depression and alleviate premenstrual syndrome symptoms by controlling related stress, such as COVID-19 stress, in preparation for the outbreak of new infectious diseases and pandemic situations such as COVID-19.

• Journal of the Korean Applied Science and Technology
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• v.39 no.2
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• pp.193-204
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• 2022

This study was to identify factors related to the experience of violence damage, focusing on health risk behavior of multi-cultural and general adolescents in Korea. Data from the 15th online survey of youth health behavior (2019) was used to analyze 35,574 adolescents. The data were analyzed using complex sample analysis by using SPSS/Win 22.0. The multi-cultural adolescents had significantly higher experience of smoking and habitual drug use and violence damage than those ordinary adolescents. In both multi-cultural and general adolescents, the more they did not live with their family, the more they had smoking experience and habitual drug use experience, the higher they experienced violence. The experience of violence victimization of general youth was significantly related to gender, school, economic status, and drinking experience. To prevent the violence victimization of adolescents, social support for adolescents who do not live with their families is required, and careful attention is needed to adolescents who have smoking and drug use experience. In addition, since multi-cultural youth are more vulnerable to health risk behaviors and violence damage than general youth, educational programs are needed to induce multi-cultural youth to practice correct health behaviors and prevent experiences of violence.

• Journal of the Korean Applied Science and Technology
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• v.39 no.2
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• pp.205-219
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• 2022

This study is a descriptive research study to understand the effects of nurses' perception of the disabled, contact experience with the disabled, and human rights sensitivity on attitudes toward the disabled. The subjects of the study were 181 nurses working at two university hospitals in D City and one university hospital in C Special Self-Governing City. Data were collected from September 2, 2021 to October 6, 2021. The collected data wre analyzed using IBM SPSS 26.0 program, and analyzed with t-test, ANOVA, Pearson's Correlation Coefficients, and Multiple Regression Analysis. Factors affecting the subject's attitude toward people with disabilities were perception of people with disabilities(t=-3.96, p<.001), presence or absence of contact with patients with disabilities(t=3.23, p=.001), and the ability to perceive responsibility among human rights sensitivity(t=2.13, p=.035), whether or not they had completed education for improvement the awareness of the disabilities(t=2.90, p=.004), and whether they were recognized of the Act on the Right to Health and Medical Accessibility for the Disabled(t=2.44, p=.016), which showed 30% of explanatory power(F=9.36, p<.001). Accordingly, it is necessary to develop an educational program that can foster nurses' attitudes toward the disabled, including awareness of the disabled, human rights sensitivity, the Health Rights Act for the Disabled, and the experience of contact with the disabled.

• Journal of the Korean Applied Science and Technology
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• v.40 no.2
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• pp.199-209
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• 2023

This study is a descriptive research to identify the factors that influence blood donation intentions of the middle-aged firefighters and prison officer based on Ajzen's (1991) planned behavior theory. The subjects of the study were 223 middle-aged firefighters and prison officer at a fire station and prison located in G City and District B. The Data were analyzed by descriptive statistics and t-test, one-way ANOVA, Pearson's correlation coefficient, Turkey, and multiple regression with the SPSS 21.0 program. There were statistically significant differences in blood donation intention according to the blood donation experience, attempted blood donation within a year, participate plan in blood donation within 3 months. The blood donation intention of middle aged showed significant positive correlations with attitude, subjective norms, and perceived behavioral control towards blood donation. Multiple regression analysis for blood donation intention revealed that the significant predictors were participate plan in blood donation within 3 months, perceived behavior control, subjective norms, attitude towards blood donation, and attempted blood donation within a year. These factors explained 69% of the variance. In order to enhance the middle aged's intention to blood donation, we need a program that can improve middle aged's attitude, subjective norms, perceived behavior control.

• Journal of Practical Agriculture & Fisheries Research
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• v.20 no.2
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• pp.39-47
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• 2018

This study was carried out to investigate the applicability of alternating temperature control during night time in a nursery seedling production. Three groups of samples were exposed to three different environmental conditions for 6 days from 18:00 to 09:00; one was constantly 15 ℃, another was alternating between 15 ℃ for 2 hours and 11 ℃ for 2 hours, and the other was alternating between 15 ℃ for 2 hours and 11 ℃ for 4 hours. Leaf temperature and stem temperature was measured in real time. The influence of cold stress was analyzed by flavonoid content and growth of tomato seedling. The temperature of leaves and stem became equal to the ambient temperature over time, furthermore, there was no significant difference among the treatments. In conclusion, it is considered that heating costs can be saved considerably, as the alternating temperature has fewer effects on cold stress reaction by tomato seedling growth.