• Title/Summary/Keyword: Nuclear technique

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Factors Affecting the Efficiency of Animal Cloning by Somatic Cell Nuclear Transfer

  • Kim, Min-Goo;Park, Chi-Hun;Lee, Sang-Goo;Seo, Hee-Won;Choi, Yo-Han;Lee, Chang-Kyu;Ka, Hak-Hyun
    • Journal of Embryo Transfer
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    • v.23 no.2
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    • pp.67-76
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    • 2008
  • Since the birth of Dolly using fully differentiated somatic cells as a nuclear donor, viable clones were generated successfully in many mammalian species. These achievements in animal cloning demonstrate developmental potential of terminally differentiated somatic cells. At the same time, the somatic cell nuclear transfer (SCNT) technique provides the opportunities to study basic and applied biosciences. However, the efficiency generating viable offsprings by SCNT remains extremely low. There are several explanations why cloned embryos cannot fully develop into viable animals and what factors affect developmental potency of reconstructed embryos by the SCNT technique. The most critical and persuasive explanation for inefficiency in SCNT cloning is incomplete genomic reprogramming, such as DNA methylation and histone modification. Numerous studies on genomic reprogramming demonstrated that incorrect DNA methylation and aberrant epigenetic reprogramming are considerably correlated with abnormal development of SCNT cloned embryos even though its mechanism is not fully understood. The SCNT technique is useful in cloning farm animals because pluripotent stem cells are not established in farm animal species. Therapeutic cloning combined with genetic manipulation will help to control various human diseases. Also, the SCNT technique provides a chance to overcome excessive demand for the organs by production of transgenic animals as xenotransplantation resources. Here, we describe the factors affecting the efficiency of generating cloned farm animals by the SCNT technique and discuss future directions of animal cloning by SCNT to improve the cloning efficiency.

ESTIMATION OF LOCAL LIQUID FILM THICKNESS IN TWO-PHASE ANNULAR FLOW

  • Lee, Bo-An;Yun, Byong-Jo;Kim, Kyung-Youn;Kim, Sin
    • Nuclear Engineering and Technology
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    • v.44 no.1
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    • pp.71-78
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    • 2012
  • In many semi-empirical analyses of flow boiling heat transfer, an annular flow is often assumed as a model flow and the local liquid film thickness is a key parameter in the analysis. This work considers a simple electrical conductance technique to estimate the local liquid film thickness in two-phase annular flows. In this approach, many electrodes are mounted flush with the inner wall of the pipe. Voltage differences between two neighboring electrodes for concentric annular flows with various liquid film thicknesses are obtained before the main experiments and logged in a look-up table. For an actual application in the annual flow, voltage differences of neighboring electrodes are measured and then corresponding local film thicknesses are determined by the interpolation of the look-up table. Even though the proposed technique is quite simple and straightforward, the numerical and static phantom experiments support its usefulness.

The Study of Improvement in Reactor Thermal Power Measurement Method using KALMAN FILTER (KALMAN FILTER를 이용한 원자로 열출력측정 방법개선에 관한 고찰)

  • 정남교
    • Journal of the Korean Professional Engineers Association
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    • v.30 no.5
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    • pp.82-95
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    • 1997
  • A Study of Improvement in Reactor Thermal Power Measurement Method using Kalman Filter. The objectives of the safety analysis of nuclear power plants are to maintain the surface temperature of fuel and fuel cladding within limit value in case of Loss of Coolant accident (LOCA) so that it ensures the safety and reliability of nuclear power plants. The new technique evaluating the reactor power and improvement of existing plant system increase the safety margin of nuclear power plant operation, and accordingly, economic effect will be anticipated. Hereby, 1 would like to introduce reactor power measurement method using Kalman filter that enables to calculate the reactor power more precisely combining the parameters, for example, turbine output as the 1 st stage pressure of high pressure turbine, and reactor power using energy equilibrium relation. It is expected that the new technique will enhance the accuracy of measurement of reactor power and maintain the reliability of nuclear power operation by increasing operational safety margin, and gain the economic benefit

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Inspection of Calandria Reactor Surface of Wolsung Nuclear Power Plant using Thermal Infrared Camera mounted on the Mobile Robot KAEROT/m2

  • Cho, Jai-Wan
    • Proceedings of the KSRS Conference
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    • 2002.10a
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    • pp.578-578
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    • 2002
  • Thermal infrared imaging is a highly promising technology for condition monitoring and predictive maintenance of electronic, electrical and mechanical elements in nuclear power plants. However, conventional low-cost infrared imaging systems suffer from poor spatial resolution compared to commercial CCD cameras. This paper describes an approach to enhance inspection performances for calandria reactor area of Wolsung nuclear power plant through the technique of superimposing thermal infrared image into real CCD image. In the occurrence of thermal abnormalities on observation points and areas of calandria reactor area, unusual hot image taken from thermal infrared camera is mapped upon real CCD image. The performance of the technique has been evaluated in the experiment carried out at Wolsung nuclear power plant in the overhaul period. The results show that localizations of thermal abnormalities on calandria reactor face can be estimated accurately.

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Mechanochemical Approach for Oxide Reduction of Spent Nuclear Fuels for Pyroprocessing

  • Kim, Sung-Wook;Han, Seung Youb;Jang, Junhyuk;Jeon, Min Ku;Choi, Eun-Young
    • Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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    • v.19 no.2
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    • pp.255-266
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    • 2021
  • Solid-state mechanochemical reduction combined with subsequent melting consolidation was suggested as a technical option for the oxide reduction in pyroprocessing. Ni ingot was produced from NiO as a starting material through this technique while Li metal was used as a reducing agent. To determine the technical feasibility of this approach for pyroprocessing, which handles spent nuclear fuels, thermodynamic calculations of the phase stabilities of various metal oxides of U and other fission elements were made when several alkaline and alkali-earth metals were used as reducing agents. This technique is expected to be beneficial, not only for oxide reduction but also for other unit processes involved in pyroprocessing.