• Asian-Australasian Journal of Animal Sciences
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• 제30권12호
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• pp.1679-1683
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• 2017

Objective: An experiment was conducted to study the association between the single nucleotide polymorphisms (SNPs) in 5'-untranslated regions (5'-UTR) of equine chorionic gonadotropin (eCG) genes and the serum eCG levels. Methods: SNPs in 5'-UTR of eCG genes were screened across 10 horse breeds, including 7 Chinese indigenous breeds and 3 imported breeds using iPLEX chemistry, and the association between the serum eCG levels of 174 pregnant Da'an mares and their serum eCG levels (determined with ELISA) was analyzed. Results: Four SNPs were identified in the 5'-UTR of the $eCG{\alpha}$ gene, and one of them was unique in the indigenous breeds. There were 2 SNPs detected at the 5' end of the $eCG{\beta}$ subunit gene, and one of them was only found in the Chinese breeds. The SNP g.39948246T>C at the 5'-UTR of $eCG{\alpha}$ was associated significantly with eCG levels of 75-day pregnant mare serum (p<0.05) in Da'an mares. Prediction analysis on binding sites of transcription factors showed that the g.39948246T>C mutation causes appearance of the specific binding site of hepatocyte nuclear factor 3 forkhead homolog 2 (HFH-2), which is a transcriptional repressor belonging to the forkhead protein family of transcription factors. Conclusion: The SNP g.39948246T>C at the 5'-UTR of $eCG{\alpha}$ is associated with eCG levels of 75-day pregnant mare serum (p<0.05).

• Journal of Chest Surgery
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• 제36권4호
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• pp.289-292
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• 2003

폐에 발생하는 원발성 악성 흑색종은 매우 드문 종양으로 문헌상으로 수 십 예의 결과만 보고되고 있다. 본 저자는 우측폐중엽에 발생한 원발성 악성 흑색종 1예를 치험하였는데 환자는 65세 된 남자로 객담을 동반한 기침으로 내원하여 컴퓨터단층촬영 소견상 우측폐 중엽에 종괴가 관찰되었으며 경피적 폐생검상 악성 흑색종이 확인되었고 bone scan상전이 소견은 없었으며 피부 및 안구 검사상 전이의 증거가 없었다. 환자는 원발성 악성 흑색종으로 진단하고 우측폐 중엽 절제술을 시행하였는데 약 4.5$\times$3 cm의 종괴가 우측폐 중엽에서 관찰되었으며 주변의 임파절을 함께 박리 하였다. 수술 중 특이 소견은 없었으며 수술 3일째 흉관을 제거할 수 있었고 병리조직 검사상 기관지 주변부의 임파절 전이가 확인되어 보조적 방사선 치료를 기획하고 수술 10일째 퇴원하였다. 환자는 현재 24개월째 외래를 통해 추적관찰 중인데 재발 및 전이의 증거 없이 지내고 있다.

• 대한세포병리학회지
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• 제18권1호
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• pp.87-91
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• 2007

Langerhans cell histiocytosis (LCH) is a pleomorphic disease entity characterized by local or disseminated atypical Langerhans cells (LCs) found most commonly in bone, lungs, mucocutaneous structures and endocrine organs. Among different sites, unifocal disease confined to a lymph node (LN) is rare. We report a case of LCH confined to a LN in a 38-year-old male who presented with a 2 cm-sized cervical mass. The fine needle aspiration (FNA) smears of cervical LN showed high cellularity having isolated LCs with contorted nuclei and nuclear grooves mixed in multinucleated giant cells, small lymphocytes and eosinophils. Charcot-Leyden crystals were also seen, as were a few dendritic-like cells and intranuclear inclusions. Confirmation of LCH was made by histopathologic studies, positive reactions for S-100 protein and CD1a immunohistochemical staining and by the demonstration of Birbeck granules on electron microscopy. The differentials to be considered include dermatopathic lymphadenitis, sinus histiocytosis with massive lymphadenopathy, Hodgkin's lymphoma and malignant histiocytosis. The characteristic cytomorphologic pattern of LCH in a LN FNA smear plays an important role in suggesting the diagnosis of LCH.

• Molecules and Cells
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• 제43권6호
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• pp.551-571
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• 2020

Nuclear receptor-related 1 (Nurr1) protein has been identified as an obligatory transcription factor in midbrain dopaminergic neurogenesis, but the global set of human NURR1 target genes remains unexplored. Here, we identified direct gene targets of NURR1 by analyzing genome-wide differential expression of NURR1 together with NURR1 consensus sites in three human neural stem cell (hNSC) lines. Microarray data were validated by quantitative PCR in hNSCs and mouse embryonic brains and through comparison to published human data, including genome-wide association study hits and the BioGPS gene expression atlas. Our analysis identified ~40 NURR1 direct target genes, many of them involved in essential protein modules such as synapse formation, neuronal cell migration during brain development, and cell cycle progression and DNA replication. Specifically, expression of genes related to synapse formation and neuronal cell migration correlated tightly with NURR1 expression, whereas cell cycle progression correlated negatively with it, precisely recapitulating midbrain dopaminergic development. Overall, this systematic examination of NURR1-controlled regulatory networks provides important insights into this protein's biological functions in dopamine-based neurogenesis.

• The Korean Journal of Physiology and Pharmacology
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• 제5권6호
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• pp.521-527
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• 2001

Diclofenac, a phenylacetic acid derivative, is a widely used non-steroidal anti-inflammatory drug (NSAID) to provide effective relief of inflammation and pain. Nitric oxide (NO) synthesized by inducible nitric oxide synthase (iNOS) has been implicated as a mediator of inflammation. We examined the inhibitory effects of diclofenac on the induction of iNOS in RAW 264.7 macrophages which were activated with lipopolysaccharide (LPS) plus interferon-gamma $(IFN-{\gamma}).$ Treatment of RAW 264.7 cells with diclofenac and other NSAIDs (aspirin and indomethacin) significantly inhibited NO production and iNOS protein expression induced by LPS plus $IFN-{\gamma}.$ Also, diclofenac but not aspirin and indomethacin, inhibited iNOS mRNA expression and nuclear factor-kappa B $(NF-{\kappa}B)$ binding activity concentration-dependently. Furthermore, transfection of RAW 264.7 cells with iNOS promoter linked to a CAT reporter gene revealed that only diclofenac inhibited the iNOS promoter activity induced by LPS plus $IFN-{\gamma}$ through the $NF-{\kappa}B$ sites of iNOS promoter. Taken together, these suggest that diclofenac may exert its anti-inflammatory effect by inhibiting iNOS gene expression at the transcriptional level through suppression of $NF-{\kappa}B$ activation.

• Journal of Radiation Protection and Research
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• 제32권2호
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• pp.71-78
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• 2007

본 연구에서는 원자력 발전소 주변 일시거주자의 방사선 비상시 소개와 관련된 사회행동 특성-특히 소개와 관련된 행동특성을 분석하는데 목적이 있다. 이를 위해 먼저, 이론적 검토 차원에서 비상사태시 소개의 의미와 유형, 그리고 소개를 시작하는데 소요되는 소개준비시간을 살펴보았다. 그리고 소개 대상 주민에 대한 행동특성을 울진, 월성 및 고리원전 방사선비상계획 구역 내에 체류하는 일시거주자를 대상으로 면접설문조사를 통해 실증 분석하였다. 연구의 주요 결과는 다음과 같다. 첫째, 방사선 비상시의 원활한 소개 및 안전 확보를 위하여 일시 거주자에게 소개 경보에 대한 사전 지식을 효율적으로 제공하기 위한 방안을 마련하는 것이 필요하다. 둘째, 방사선 비상시 일시거주자의 교육 훈련 방안을 마련하는 것이 필요하다. 셋째, 일시거주자가 자발적 소개를 하지 않으려는 이유와 배경을 파악하고, 대응 방안을 마련하는 것이 필요하다.

• Journal of Periodontal and Implant Science
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• 제49권5호
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• pp.319-329
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• 2019

Purpose: Direct application of atmospheric-pressure plasma jets (APPJs) has been established as an effective method of microbial decontamination. This study aimed to investigate the bactericidal effect of direct application of an APPJ using helium gas (He-APPJ) on Porphyromonas gingivalis biofilms on sandblasted and acid-etched (SLA) titanium discs. Methods: On the SLA discs covered by P. gingivalis biofilms, an APPJ with helium (He) as a discharge gas was applied at 3 different time intervals (0, 3, and 5 minutes). To evaluate the effect of the plasma itself, the He gas-only group was used as the control group. The bactericidal effect of the He-APPJ was determined by the number of colony-forming units. Bacterial viability was observed by confocal laser scanning microscopy (CLSM), and bacterial morphology was examined by scanning electron microscopy (SEM). Results: As the plasma treatment time increased, the amount of P. gingivalis decreased, and the difference was statistically significant. In the SEM images, compared to the control group, the bacterial biofilm structure on SLA discs treated by the He-APPJ for more than 3 minutes was destroyed. In addition, the CLSM images showed consistent results. Even in sites distant from the area of direct He-APPJ exposure, decontamination effects were observed in both SEM and CLSM images. Conclusions: He-APPJ application was effective in removing P. gingivalis biofilm on SLA titanium discs in an in vitro experiment.

• 방사성폐기물학회지
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• 제17권2호
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• pp.203-212
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• 2019

처분 부적합 폐기물이란 원전운영이나 해체 시 처리, 고화 및 포장이 요구되는 방사성폐기물 등을 일컬으며, 대표적으로 분산 특성을 갖는 입자성 방사성폐기물을 예로 들 수가 있다. 이들 폐기물에는 원전 운영과정에서 발생되는 농축폐액의 건조분말, 슬러리 및 슬러지, 향후 원전 해체과정에서 발생되는 온갖 분말 상태의 폐기물(콘크리트 파쇄물, 제염 슬러지 등), 그리고 제염이 용이치 못한 미세 크기의 방사능오염 토양 등이 있다. 입자성 폐기물을 기존의 고화방식으로 처리할 경우에는 최종 폐기물의 부피가 증가하는 단점을 갖게 되어 처분 비용의 증가 및 처분장의 수용성을 감소하는 결과를 야기할 수가 있다. 따라서 이들 문제를 해결하고자 본 연구에서는 최종 폐기물 부피의 감용화를 위해 롤 압축 기술을 이용하여 분말의 펠렛화 연구를 수행하였다.

• 대한토목학회논문집
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• 제44권3호
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• pp.277-282
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• 2024

최근 기후변화로 인해 태풍의 강도와 빈도가 증가하고 있으며, 태풍은 원자력발전소의 소외전원상실(LOOP)을 발생시킬 수 있다. 따라서, 태풍 동반 강풍에 대한 소외전력망의 확률론적 안전성 평가(PSA)를 통한 대비가 필요하다. 하지만, 원자력발전소의 소외전력망에 대한 태풍 동반 강풍 PSA 연구는 미흡한 실정이다. 본 연구에서는 국내원전부지 중 소외전력망의 피해가 잦았던 고리원전부지를 대상으로 소외전력망의 태풍 동반 강풍에 의한 PSA를 수행하였다. 태풍 동반 강풍에 의한 소외전력망의 PSA를 수행하기 위해 고리원전부지의 태풍재해도를 Logic Tree와 Monte Carlo Simulation을 활용하여 도출하였다. 전력망을 구성하는 요소의 취약도를 활용하여 전력망의 취약도 분석을 수행하였다. 최종적으로 소외전력망이 원자력발전소에 전력을 공급하지 못할 확률을 정량적으로 분석하였다.

• 방사성폐기물학회지
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• 제22권3호
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• pp.313-324
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• 2024

Se sorption onto Ca-type montmorillonite purified from Bentonil-WRK-a new research bentonite introduced by Korea Atomic Energy Research Institute-was examined under ambient conditions (pH 4-9, pe 7-9, I = 0.01 M CaCl₂, and T = 25℃). Se(IV) was identified as the oxidation state responsible for weak sorption (Kd < 22 L·kg^-1) by forming surface complexes with edge functional groups of the montmorillonite. Thermodynamic modeling, considering reaction mechanisms of outer-sphere complexation (≡AlOH⁺₂ + HSeO₃^- ⇌ ≡AlOH₃SeO₃, log K = 0.50 ± 0.21), inner-sphere complexation (2≡AlOH + H₂SeO₃(aq) ⇌ (≡Al)₂SeO₃ + 2H₂O(l), log K = 7.89 ± 0.51), and Ca²⁺-involved ternary complexation (≡AlOH + Ca²⁺ + SeO₃^2- ⇌ ≡AlOHCaSeO₃, log K = 7.69 ± 0.28) between selenite and aluminol sites of montmorillonite, acceptably reproduced the batch sorption data. Outer- and inner-sphere complexes are predominant Se(IV) forms sorbed in acidic (pH ≈ 4) and near-acidic (pH ≈ 6) regions, respectively, whereas ternary complexation accounts for Se(IV) sorption at neutral pHs under the ambient conditions. The experimental and modeling data generally extend a material-specific sorption database of Bentonil-WRK, which is essential for assessing its radionuclide retention performance as a buffer candidate of deep geological disposal system for high-level radioactive waste.