• Journal of Korean Society for Atmospheric Environment
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• v.16 no.5
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• pp.453-467
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• 2000

The aim of this research is to collect and characterize fine particles (FPM:$\leq$2.5${\mu}{\textrm}{m}$) and coarse particles (CPM: 2.5~10${\mu}{\textrm}{m}$) using a low volume air sampler provided by the IAEA, at urban (Taejon) and rural area(Wonju) for a period of about two years(April 1996 to May 1998) and to promote a use of nuclear analytical techniques for air pollution studies. For the collection of airborne particulate matter (PM(sub)10), the Gent stacked filter unit sampler and polycarbonate membrane filters were employed. The concentration of trace elements in collected APM samples were determined byu instrumental Neutron Activation Analysis. For validation of the analytical data, internal quality control were implemented by using both the comparison of the analytical results of standard reference materials(NIST SRM 1648) and interlaboratory comparison for proficiency test (NAT-3). The standard uncertainty was less than 15% and Z-score of two samples were within $\pm$1. The monitoring of (PM(sub)10) mass concentration and elemental concentrations were carried out weekly. The average mass concentration of (PM(sub)10) in urban and rural areas were 59.2$\pm$36.5$\mu\textrm{g}$/㎥ and 41.4$\pm$23.7$\mu\textrm{g}$/㎥, respectively. To investigate the emission source, the enrichment factors were calculated for the fine and coarse particle fractions at two sites, respectively and these values were classified for anthropogenic and soil origin elements.

• Applied Chemistry for Engineering
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• v.16 no.1
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• pp.107-111
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• 2005

We have performed a study on the electrochemical characteristics and nuclear ($^7Li$) magnetic resonance of $V_2O_5$ xerogels that have been synthesized by the sol-gel reaction of $V_2O_5$ powder with hydrogen peroxide. NMR measurements revealed that chemical shift of $Li^+$ ions varied as lithium ions were inserted into $V_2O_5$ xerogel and that several different sites for $Li^+$ ions existed in the $V_2O_5$ xerogel structure. The electrochemical properties of the xerogel electrodes did not depend much upon the concentration of $V_2O_5$ and HCl that were used for the synthesis of $V_2O_5$ gels. The specific capacity of $V_2O_5$ xerogels were about 140 mAh/g, similar to that of the xerogels prepared by the ion exchange method.

• Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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• v.7 no.1
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• pp.33-38
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• 2009

An experiment for uranium sorption onto fresh and weathered biotites was performed. After centrifugation, concentrations of uranium in the supernatants were analyzed using ICP-MS, and biotite samples were investigated using XRD and SEM. With powdered biotites (<3 mm in size), we have conducted uranium sorption experiments about fresh and weathered biotites to obtain uranium sorption amounts in various pH conditions. The uranium sorption was not high at a low pH (e.g., pH 3), but increased with increasing pH. There were lower uranium sorption by the weathered biotites than by the fresh ones, and the difference was much larger at higher pH (e.g., pH 11). The lower sorption values of uranium by the weathered biotites may be caused by a change of mineral surfaces and a chemical behavior of surrounding dissolved elements. It seems that the uranium-mineral interaction has been diminished, especially, in the weathered biotite by a destruction and dissolution of preferential sorption sites on the mineral surfaces and by the colloidal formation from dissolved elements.

• Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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• v.17 no.4
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• pp.447-457
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• 2019

Japan enacted the "Designated Radioactive Waste Final Disposal Act" for the geological disposal of high-level radioactive waste in 2000 and began the site selection process. However, no local government wanted to participate in the siting process. Therefore, in 2015, the Japanese government developed a new site selection process during the literature survey step, and on June 28, 2017 they published a "Nationwide Map of Scientific Features for Geological Disposal" created with the aim of promoting public participation from local governments. This map illustrated the requirements and criteria to be considered in the early or conceptual stages of securing a geological repository and was useful for improving public understanding and exchanging opinions with local governments by analyzing the suitability of different geological disposal sites.

• Fisheries and Aquatic Sciences
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• v.14 no.1
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• pp.43-54
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• 2011

Gene structure of copper/zinc-superoxide dismutase (Cu/Zn-SOD; sod1) was characterized in Hemibarbus mylodon (Teleostei; Cypriniformes), an endangered freshwater fish species in Korean peninsula. Full-length cDNA of H. mylodon SOD1 consisted of a 796-bp open reading frame sequence encoding 154 amino acids, and the deduced polypeptide sequence shared high sequence homology with other orthologs, particularly with regard to metal-coordinating ligands. Genomic structure of the H. mylodon sod1 gene (hmsod1; 1,911 bp from the ATG start codon to the stop codon) was typical quinquepartite (i.e., five exons interrupted by four introns); the lengths of the exons were similar among species belonging to various taxonomic positions. The molecular phylogeny inferred from sod1 genes in the teleost lineage was in accordance with the conventional taxonomic assumptions. 5'-flanking upstream region of hmsod1, obtained using the genome walking method, contained typical TATA and CAAT boxes. It also showed various transcription factor binding motifs that may be potentially involved in stress/immune response (e.g., sites for activating proteins or nuclear factor kappa B) or metabolism of xenobiotic compounds (e.g., xenobiotic response element; XRE). The hmsod1 transcripts were ubiquitously detected among tissues, with the liver and spleen showing the highest and lowest expression, respectively. An experimental challenge with Edwardsiella tarda revealed significant upregulation of the hmsod1 in kidney (4.3-fold) and spleen (3.1-fold), based on a real-time RT-PCR assay. Information on the molecular characteristics of this key antioxidant enzyme gene could be a useful basis for a biomarker-based assay to understand cellular stresses in this endangered fish species.

• Journal of the Korean Magnetic Resonance Society
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• v.2 no.2
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• pp.141-151
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• 1998

Human annexin I is a member of annexin family of calcium dependent phospholipid binding proteins, which have been implicated in various physiological roles including phospholipase A2(PLA2) inhibition, membrane fusion and calcium channel activity. In this work, the structure of N-terminally truncated human annexin I ({{{{ DELTA }}-annexin I) and its interactions with Ca2+, ATP and cAMP were studied at atomic level by using nuclear magnetic resonance (NMR) spectroscopy. The effect of Ca2+ binding on the structure of {{{{ DELTA }}-annexin I was investigated. The addition of Ca2+ to {{{{ DELTA }}-annexin I caused some changes in 13C NMR spectra. Carbonyl carbon resonances of some histidines were significantly broadened by Ca2+ binding. However, in the case of methionine, phenylalanine, and tyrosin, small changes could be observed. We found that ATP and cAMP bind {{{{ DELTA }}-annexin I, and the binding ratio of ATP to {{{{ DELTA }}-annexin I is 1. These results are well consistent with the report that cAMP and ATP interact with annexin I, and affect the calcium channels formed by annexin I. Because {{{{ DELTA }}-annexin I is a large protein with 35 kDa molecular weight, site-specific (carbonyl-13C) labeling technique was used to study the interaction sites of {{{{ DELTA }}-annexin I with Ca2+. NMR study was focused on the carbonyl carbon resonances of tyrosine, phenylalanine, methionine and histidine residues of {{{{ DELTA }}-annexin I because the number of these amino acids is small in the amino acid sequence of {{{{ DELTA }}-annexin I.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.10a
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• pp.25-25
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• 2018

The Fukushima Daiichi Nuclear Power Plant accident in March 2011 caused severe radioactive contamination in the surrounding environment. Since the accident, much attention has been paid to the biological and genetic consequences of organism inhabiting the contaminated area. The effect of radiation exposure on genetic mutation rates is little known, especially for low doses and in situ conditions. Evaluating DNA mutation by low levels of radiation dose is difficult due to the rare mutation event and lack of sequence information before the accident. In this study, correlations with air dose levels and somatic DNA mutation rates were evaluated using Next Generation Sequencer for the clonal plant, Phyllostachys edulis. This bamboo is known to spread an identical clone throughout Japan, and it has the advantage that we can compare genetic mutation rate among identical clone growing different air dose levels. We collected 94 samples of P. edulis from 14 sites with air dose rates from $0.04{\sim}7.80{\mu}Gy/h$. Their clonal identity was confirmed by analysis using 24 microsatellite markers, and then, sequences among samples were compared by MIG sequence. The sequence data were obtained from 2,718 loci. About ~200,000 bp sequence (80 bp X 2,718 loci) were obtained for each sample, and this corresponds to about 0.01% of the genome sequence of P. edulis. In these sequences, 442 loci showed polymorphism patterns including recent origin mutation, old mutation, and sequence errors. The number of mutations per sample ranged from 0 to 13, and did not correlate with air dose levels. This result indicated that DNA mutations have not accumulated in P. edulis living in the air doses levels less than $10{\mu}Gy/h$. Our study also suggests that mutation rates can be assessed by selecting an appropriate experimental approach and analyzing with next generation sequencer.

• Journal of the Institute of Electronics and Information Engineers
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• v.50 no.2
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• pp.60-69
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• 2013

Highlighted by recent security breaches including Google, Western Energy Company, and the Stuxnet infiltration of Iranian nuclear sites, Cyber warfare attacks pose a threat to national and global security. In particular, targeted attacks such as APT exploiting a high degree of stealthiness over a long period, has extended their victims from PCs and enterprise servers to government organizations and critical national infrastructure whereas the existing security measures exhibited limited capabilities in detecting and countermeasuring them. As a solution to fight against such attacks, we designed and implemented a security monitoring system, which shares security information and helps cooperative countermeasure. The proposed security monitoring system collects security event logs from heterogeneous security devices, analyses them, and visualizes the security status using 3D technology. The capability of the proposed system was evaluated and demonstrated throughly by deploying it under real network in a ISP for a week.

• Journal of Radiation Protection and Research
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• v.19 no.2
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• pp.109-120
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• 1994

A method for estimating the effectiveness of each protective action against a nuclear accident has been proposed using the fuzzy set theory. In most of the existing countermeasure models in actions under radiological emergencies, the large variety of possible features is simplified by a number of rough assumptions. During this simplification procedure, a lot of information is lost which results in much uncertainty concerning the output of the countermeasure model. Furthermore, different assumptions should be used for different sites to consider the site specific conditions. Tn this study, the diversity of each variable related to protective action has been modelled by the linguistic variable. The effectiveness of sheltering and evacuation has been estimated using the proposed method. The potential advantage of the proposed method is in reducing the loss of information by incorporating the opinions of experts and by introducing the linguistic variables which represent the site specific conditions.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.10 no.2
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• pp.103-112
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• 1990

This study examines the characteristics of soil behavior which includes many uncertainties in seismic design, evaluates the dynamic soil properties and studies the soil-structure interaction to generalize the applicability and economy of the available sites. An example analysis is performed for soil-structure system response assuming a containment structure built on site which includes soil layers using both elastic halfspace analysis and FEM analysis against the seismic loads from the actual design. This exercise is performed as a part of the safety analysis and economic assessment of the nuclear power plant built on soils. It includes the preparation of computer program capable of incorporating large nonlinearity in the analysis, resonable evaluation procedures to determine input soil data. Nonlinear FEM analysis of Seed and Idriss model is found suitable for the accurate analysis of dynamic response of soils. Linear FEM analysis using dynamic soil properties at strain level obtained by one-dimensional seismic response, and elastic half-space analysis using dynamic soil properties at strain level under static loads are recommended to evaluate the dynamic soil properties.