• International Journal of Industrial Entomology and Biomaterials
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• 제13권1호
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• pp.31-35
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• 2006

The ubiquitin conjugating enzyme 2 (E2) is core component of ubiquitin proteasome pathway (UPP) which represents a selective mechanism for intracellular proteolysis in eukaryotic cells. The E2 has been implicated in the intracellular transfer of ubiquitin to target protein. We show here the involvement of E2 in antiviral immune of Bombyx mori to Bombyx mori nuclear polyhedrosis virus (BmNPV). In this study, mRNA fluorescent differential display PCR (FDD-PCR) was performed with BmNPV highly resistant silkworm strain NB and susceptible silkworm strain 306. At 24 h post BmNPV infection, FDD-PCR with the arbitrary primer AP34 showed that one cDNA band was down-regulated in the midgut of resistant strain, but highly expressed in susceptible strain. The deduced amino acid sequence of this cDNA clone share 99% identity with the recently published B. mori ubiquitin conjugating enzyme E2 (Genbank NO: DQ311351). Fluorescent quantitative PCR corroborated down regulation of E2 in resistant strain. We there conclude that BmNPV infection evokes strong response of susceptible strain including activation of UPP. BmNPV may evolve escape mechanisms that manipulate the UPP in order to persist in the infected host. In addition, the identification of down-regulation of E2 in resistant strain, as well as structure data, are essential to understanding how UPP operates in silkworm antiviral immune to BmNPV disease.

• 한국지구과학회지
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• 제31권7호
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• pp.673-680
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• 2010

교량 등 대규모 토목 구조물의 깊은 기초로 빈번하게 사용되는 강관말뚝은 상부 하중을 하부의 견고한 기초지반에 전달하여 구조물을 안전하게 지지하는 역할을 한다. 이러한 강관말뚝은 지중에 시공되므로 상세 시공정보가 없는 기존 말뚝기초의 깊이를 추후에 파악하기란 쉽지 않은 일이다. 그러나 기존 구조물의 보강공사 또는 신규 공사시 인접한 기존 구조물의 안정성 확보를 위해 기 시공된 말뚝의 깊이를 사전에 파악하는 문제는 매우 중요한 요소이다. 본 연구에서는 토목공사현장에서 흔히 사용되는 강관말뚝의 특성을 고려하여 강관말뚝의 깊이와 위치 등의 정보를 시추공 플럭스게이트 3성분 자력계를 통해 알아낼 수 있는지를 조사하였다. 이전 연구들은 말뚝기초의 깊이를 자력값의 수직성분의 측정으로 효과적으로 알아낼 수 있음을 보였으며 이를 현장자료에 적용하여서 그 적용성을 입증한 바 있으나, 본 논문에서는 시추공 3성분 자력계를 효과적으로 이용한다면 깊이에 대한 정보뿐 아니라 위치에 대한 정보까지도 얻을 수 있음을 보였다.

• 한국전산유체공학회지
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• 제21권4호
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• pp.33-39
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• 2016

Generally, thermal analysis of spent fuel storage cask has been conducted using the porous media and effective thermal conductivity model to simplify the structural complexity of spent fuel assemblies. As the fuel assembly is composed of two regions; active fuel region corresponding to UO2 pellets and unactive fuel region corresponding to the top and bottom nozzle, the heat transfer performance can be influenced depending on porous media application at these regions. In this study, numerical analysis on concrete storage cask of spent fuel was performed to investigate heat transfer effects for two cases; one was porous media application only to active fuel region(case 1) and the other one was porous media to whole length of fuel assembly(case 2). Using computational fluid dynamics code, the three dimensional, 1/4 symmetry model was constructed. For two cases, maximum temperatures for each component were evaluated below the allowable limits. For the case 1, maximum temperatures for fuel cladding, neutron absorber and baskets inside the canister were slightly higher than those for the case 2. In particular, even though the helium flows with low velocity due to buoyant forces occurred at the top and bottom of unactive fuel region, treating only active fuel region as the porous media was ineffective in respect of the heat removal performance of concrete storage cask, implying a conservative result.

• 대한기계학회논문집A
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• 제40권2호
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• pp.167-173
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• 2016

본 논문은 유한요소해석을 이용한 고변형률 조건에서의 연성파손 해석기법을 제안한다. 고변형률 하중이 작용하는 구조물에 대한 파괴거동 예측을 위해 본 논문에서는 Johnson/Cook 모델을 고려한 수정응력 파괴변형률 모델을 사용하였다. 제시된 모델은 인장 실험 모사해석결과로부터 얻어지는 삼축응력 및 파괴변형률에 의해 파손이 정의된다. 다양한 실험속도의 인장 실험결과 및 정적 하중조건에서의 파괴인성 실험을 이용하여 수정응력 파괴변형률 모델의 변수를 결정하였다. 결정된 모델을 이용하여 동적하중조건에서 파괴인성시편에 대한 해석을 수행하였으며 해석결과와 실험결과를 비교하여 해석기법을 검증하였다.

• Biomolecules & Therapeutics
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• 제19권3호
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• pp.288-295
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• 2011

Amyloid beta ($A{\beta}$)-induced neurotoxicity is a major pathological mechanism of Alzheimer's disease (AD). In this study, we investigated the inhibitory effect of L-theanine, a component of green tea (Camellia sinensis) on $A{\beta}_{1-42}$-induced neurotoxicity and oxidative damages of macromolecules. L-theanine inhibited $A{\beta}_{1-42}$-induced generation of reactive oxygen species, and activation of extracellular signal-regulated kinase and p38 mitogenic activated protein kinase as well as the activity of nuclear factor kappa-B. L-theanine also signifi cantly reduced oxidative protein and lipid damage, and elevated glutathione level. Consistent with the reduced neurotoxic signals, L-theanine (10-50 ${\mu}g$/ml) concomitantly attenuated $A{\beta}_{1-42}$ (5 ${\mu}M$)-induced neurotoxicity in SK-N-MC and SK-N-SH human neuroblastoma cells. These data indicate that L-theanine on $A{\beta}$-induced neurotoxicity prevented oxidative damages of neuronal cells, and may be useful in the prevention and treatment of neurodegenerative disease like AD.

• 비파괴검사학회지
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• 제31권5호
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• pp.536-542
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• 2011

원자력발전소의 이종금속 용접부는 PWSCC 결함에 민감한 것으로 알려져 있으며 기량검증된 검사자가 기량검증된 절차서를 사용하여 가동중검사 기간 중에 주기적인 검사를 수행하고 있다. 국내 원자력발전소 이종금속 용접부의 형상 조사 결과에 따르면 대부분의 이종금속 용접부가 경사진 노즐부나 인접부에 위치하는 것으로 나타났다. 일반적인 초음파 탐촉자를 사용하여 경사부위에 위치한 이종금속 용접부의 검사를 수행할 경우 초음파 탐촉자의 접근성이 제한되어 검사체적을 모두 검사하기가 어렵다. 특히 축방향 결함 검출을 위한 원주방향 주사에서는 초음파 탐촉자가 경사면에 위치하게 되면 반사체로부터 결함 신호를 얻기 가 어려우며 이에 따라 결함 검출이 어렵게 된다. 이러한 문제점을 극복하기 위해서는 경사면을 고려하여 비틀림 각도를 적용한 초음파 탐촉자를 사용하는 것이 필요하다. 모델링을 통하여 비틀림 굴절 종파탐촉자 를 설계하고 축방향 결함 검사용 비틀림 굴절 종파탐촉자를 제작하여 실험을 통하여 결함으로부터 신호를 취득하였다. 일반 탐촉자와 비틀림 각도가 적용된 탐촉자의 초음파 응답신호를 비교한 결과 비틀림 각도가 적용된 탐촉자의 초음파 응답신호가 훨씬 뛰어난 것으로 나타났다.

• 한국동물번식학회:학술대회논문집
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• 한국동물번식학회 2003년도 학술발표대회 발표논문초록집
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• pp.96-96
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• 2003

Nek2 (NIMA-related protein) is a mammalian cell cycle-regulated kinase that involves in chromosome condensation and centrosome regulation and NuMA (nuclear mitotic apparatus protein) is involved in spindle assembly during a cell cycle. The cellular distribution and organization of the centrosomal components is completely unknown during fertilization and embryonic development. We examined distribution of two well-known centrosomal proteins, Nek2 and NuMA in mouse gametes and embryos to get an insight in the reorganization of centrosomal proteins during germ cell development and early fertilization. Spermatogenic cells, gametes, and embryos were analyzed with anti-Nek2 or -NuMA antibodies by immunological assay, RT-PCR, and overexpression through gene transfection. Mitotically or meiotically active spermatogenic cells were intensively stained with these antibodies in both centrosomes and cytoplasm, whereas the oocytes showed different staining patterns depending on the meiotic stages. During maturation, GV, GVBD, and MI stage were clearly stained with NuMA antibody in the nucleus or cytoplasm at MII. Also, Nek2 was detectable in cytoplasm as scattered spots or chromosome associated at MII. In early developmental embryo, NuMA was detected in nucleus of each blastomere, while Nek2 was detected in cytoplasm. In contrast to previously reported results, Nek2 and NuMA were detected in both decondensing head, and the centriole of demembranated and decondensed sperm or whole body of trypsin-treated sperm for Nek2. During meiotic progress in oocytes, transcripts levels were the highest in MI stage and then downregulated in MII. Also, it shows dramatically change in early developmental embryos, firstly, it was increased until 4 cell stage and reduced in 8 cell stage, and finally, transcript levels were upregulated until blastoscyst. This finding suggests that cnetrosomal component may play an important role in reorganizing of functional centrosome during fertilization process and subsequent development.

• Computers and Concrete
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• 제7권4호
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• pp.347-364
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• 2010

Since its invention in the $19^{th}$ century, Reinforced Concrete (RC) has been widely used in the construction of a lot of different structures, as buildings, bridges, nuclear central plants, or even ships. The details of the mechanical response for this kind of structures depends directly upon the material behavior of each component: concrete and steel, as well as their interaction through the bond-slip, which makes a rigorous engineering analysis of RC structures quite complicated. Consequently, the practical calculation of RC structures is done by adopting a lot of simplifications and hypotheses validated in the elastic range. Nevertheless, as soon as any RC structural element is working in the inelastic range, it is possible to obtain the numerical prediction of its realistic behavior only through the use of non linear analysis. The aim of this work is to develop a new kind of Finite Element: the "Enhanced Solid Element (ESE)" which takes into account the complex composition of reinforced concrete, being able to handle each dissipative material behavior and their different deformations, and on the other hand, conserving a simplified shape for engineering applications. Based on the recent XFEM developments, we introduce the concept of nodal enrichment to represent kinematics of steel rebars as well as bonding. This enrichment allows to reproduce the strain incompatibility between concrete and steel that occurs because of the bond degradation and slip. This formulation was tested with a couple of simple examples and compared to the results obtained from other standard formulations.

• Fisheries and Aquatic Sciences
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• 제15권2호
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• pp.137-143
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• 2012

The abalone Haliotis discus hannai, is one of the economically important species in the fisheries industry. Abalone intestines are one of the by-products of its processing. To investigate its bioactive potential, abalone intestine was digested using an in vitro gastrointestinal (GI) digestion system containing pepsin, trypsin, and ${\alpha}$-chymotrypsin. The abalone intestine G1 digests (AIGIDs) produced by the GI digestion system were fractionated into AIGID I (> 100 kDa), AIGID II (10-100 kDa), and AIGID III (1-10 kDa) using an ultrafiltration membrane system. Of the three digests, AIGID II and AIGID III exhibited inhibitory effects against matrix metalloproteinase-2 and -9 (MMP-2, MMP-9) in HT1080 human fibrosarcoma cells. Both fractions potently inhibited gelatine digestion by MMP-2 and MMP-9 treated with phorbol 12-myristate 13-acetate (PMA) and migration of HT1080 cells in dose dependently. Furthermore, AIGID II and III attenuated expression of p65, a component of nuclear transcription factor kappa B. These results indicate that of the abalone intestine digests inhibit MMP-2 and MMP-9. Thus, the AIGIDs or their active components may have preventive and therapeutic potential for diseases associated with MMP-2 and MMP-9 activation in fibrosarcoma cells.

• Journal of Korean Neurosurgical Society
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• 제49권1호
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• pp.8-12
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• 2011

Objective: Ossification of the posterior longitudinal ligament (OPLL) has a strong genetic component. Specific gene polymorphisms may be associated with OPLL in several genes which regulate calcification in chondrocytes, change of extracellular collagen matrix and secretions of many growth factors and cytokines controlling bone morphogenesis. Toll-like receptor 5 (TLR5) may playa role in the pathogenesis of OPLL by intermediate nuclear factor-kappa B (NF-${\kappa}B$). The current study focused on coding single nucleotide polymorphisms (SNPs) of TLR5 for a case-control study investigating the relationship between TLR5 and OPLL in a Korean population. Methods: A total of 166 patients with OPLL and 231 controls were recruited for a case-control association study investigating the relationship between SNPs of TLR5 gene and OPLL. Four SNPs were genotyped by direct sequencing (rs5744168, rs5744169, rs2072493, and rs5744174). SNP data were analyzed using the SNPStats, SNPAnalyzer, Haploview, and Helixtree programs. Multiple logistic regression analysis with adjustment for age and gender was performed to calculate an odds ratio (OR). Results: None of SNPs were associated with OPLL in three alternative models (codominant, dominant, and recessive models; p> 0.05). A strong linkage disequilibrium block, including all 4 SNPs, was constructed using the Gabriel method. No haplotype was significantly associated with OPLL in three alternative models. Conclusion: These results suggest that Toll-like receptor 5 gene may not be associated with ossification of the posterior longitudinal ligament risk in Korean population.