• 제목/요약/키워드: NuMA

검색결과 9건 처리시간 0.025초

Functional Analyses of Centrosomal Proteins, Nek2 and NuMA in Development of Mouse Gametes and Early Embryos

  • Youn, Hong-Hee;Oh, Hwa-Soon;Lee, Kwang-Hee;Son, Chae-Ick;Lee, Sang-Ho
    • 한국동물번식학회:학술대회논문집
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    • 한국동물번식학회 2003년도 학술발표대회 발표논문초록집
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    • pp.96-96
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    • 2003
  • Nek2 (NIMA-related protein) is a mammalian cell cycle-regulated kinase that involves in chromosome condensation and centrosome regulation and NuMA (nuclear mitotic apparatus protein) is involved in spindle assembly during a cell cycle. The cellular distribution and organization of the centrosomal components is completely unknown during fertilization and embryonic development. We examined distribution of two well-known centrosomal proteins, Nek2 and NuMA in mouse gametes and embryos to get an insight in the reorganization of centrosomal proteins during germ cell development and early fertilization. Spermatogenic cells, gametes, and embryos were analyzed with anti-Nek2 or -NuMA antibodies by immunological assay, RT-PCR, and overexpression through gene transfection. Mitotically or meiotically active spermatogenic cells were intensively stained with these antibodies in both centrosomes and cytoplasm, whereas the oocytes showed different staining patterns depending on the meiotic stages. During maturation, GV, GVBD, and MI stage were clearly stained with NuMA antibody in the nucleus or cytoplasm at MII. Also, Nek2 was detectable in cytoplasm as scattered spots or chromosome associated at MII. In early developmental embryo, NuMA was detected in nucleus of each blastomere, while Nek2 was detected in cytoplasm. In contrast to previously reported results, Nek2 and NuMA were detected in both decondensing head, and the centriole of demembranated and decondensed sperm or whole body of trypsin-treated sperm for Nek2. During meiotic progress in oocytes, transcripts levels were the highest in MI stage and then downregulated in MII. Also, it shows dramatically change in early developmental embryos, firstly, it was increased until 4 cell stage and reduced in 8 cell stage, and finally, transcript levels were upregulated until blastoscyst. This finding suggests that cnetrosomal component may play an important role in reorganizing of functional centrosome during fertilization process and subsequent development.

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단순 열전달 모델을 이용한 히트파이프의 열전달 성능특성에 관한 연구 (Heat transfer characteristics of the heat pipe using simplified heat transfer model)

  • 서재형;방유마;서이수;이무연
    • 한국산학기술학회논문지
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    • 제16권1호
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    • pp.15-20
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    • 2015
  • 본 연구의 목적은 전기동력 자동차의 전기-전자 장비들을 효과적으로 냉각시키면서 자체적으로 에너지 소비를 최소화 시킬 수 있는 노력의 일환으로, 단순 열전달 모델을 이용하여 윅이 있는 히트파이프의 열전달 및 유동 특성을 고찰하는 것이다. 이를 위하여 히트파이프는 COMSOL프로그램을 이용하여 해석하였고, 작동유체로 물을 이용하였다. 또한, 히트파이프의 속도 및 온도 특성을 히프파이프 길이에 따라 해석하였고, 국소 및 평균 Nu수를 계산하였다. 결과적으로, 히트파이프의 관성력은 가열면과 냉각면의 온도차에 의하여 발생하였다. 히트파이프내 열전달은 가열면에서 냉각면으로 발생하고 히트파이프 중앙으로 갈수록 증가하였다. 더불어, 가열면의 Nu수는 최대 4.47로 나타났으며, 평균 Nu수는 1.88이고, 냉각면의 Nu수는 최대 0.7로 나타났으며, 평균 Nu수는 0.1이다.

"음식보(飮食譜)" 의 조리(調理)에 관한 분석적(分析的) 고찰(考察) (Analytical Study on the Cooking in Eum Sik Bo)

  • 김귀영;이성우
    • 한국식생활문화학회지
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    • 제3권2호
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    • pp.135-142
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    • 1988
  • "Eum Sik Bo," the old cook book in Korean, had been kept on microfilm by Hwang, but the condition of copy was not clear so that interpretation of the content was very difficult. The interpreted content was classified and analyzed. The kinds of cooking food recorded in this book were alcohol beverage 12, Side dish 12, Dessert 11, etc. The materials used for the cooking foods cereals, meats, fishes, honey, vegetables, etc. - were various and the cooking methods were described in detail. But the unit of measuring and the used utensils were very poor. The special feature of "Eum Sik Bo" were the fact that red pepper was not used as seasoning and that Nu Ruk was used for Sik He and Ma ji Jub for Nu Ru Mi compared with "Eum Sik Di Mi Bang" and "Zu Bang Moon" According to these points, "Eum Sik Bo" was supposed to be written contemporary or a little later than "Eum Sik Di Mi Bang(1670's)" and "Zu Bang Moon (the end of 17th Century)."

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횡방향으로 등방성인 재료에서 균열선단 크리프 변형 거동 (Crack Tip Creep Deformation Behavior in Transversely Isotropic Materials)

  • 마영화;윤기봉
    • 대한기계학회논문집A
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    • 제33권12호
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    • pp.1455-1463
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    • 2009
  • Theoretical mechanics analysis and finite element simulation were performed to investigate creep deformation behavior at the crack tip of transversely isotropic materials under small scale creep (SCC) conditions. Mechanical behavior of material was assumed as an elastic-$2^{nd}$ creep, which elastic modulus ( E ), Poisson's ratio ( ${\nu}$ ) and creep stress exponent ( n ) were isotropic and creep coefficient was only transversely isotropic. Based on the mechanics analysis for material behavior, a constitutive equation for transversely isotropic creep behavior was formulated and an equivalent creep coefficient was proposed under plain strain conditions. Creep deformation behavior at the crack tip was investigated through the finite element analysis. The results of the finite element analysis showed that creep deformation in transversely isotropic materials is dominant at the rear of the crack-tip. This result was more obvious when a load was applied to principal axis of anisotropy. Based on the results of the mechanics analysis and the finite element simulation, a corrected estimation scheme of the creep zone size was proposed in order to evaluate the creep deformation behavior at the crack tip of transversely isotropic creeping materials.

현대공간의 전통요소에 대한 사용자 인식과 태도: 전문 디자인잡지의 기사 내용을 중심으로 (Users' Perceptions and Attitudes Regarding Use of Korean Traditional Elements in Contemporary Spaces: Focused on the Article Contents of Professional Design Magazines)

  • 이민아
    • 대한가정학회지
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    • 제48권7호
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    • pp.77-87
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    • 2010
  • This study investigated users' perceptions and attitudes toward Korean traditional elements in contemporary spaces. We collected and analyzed the article contents of two design magazines, from their issues of January 2000 through December 2009, for a total of 87 cases. The results were as follows. First, the important motives for applying traditional elements to current living spaces were individual tastes, preference for a secluded life, and the users' childhood memories. Second, the most frequently used traditional element was traditional structure. Others were traditional spatial compositions and the use of natural materials. The two main purposes for renovating a space or constructing a building were to preserve traditional ambience or to add convenience and practical use to it. Third, the most frequently mentioned advantages of traditional elements were their environment-friendly characteristics, such as connecting to nature and using natural materials. Fourth, the users preferred, in order, Ma-dang, natural environments, doors/windows of various types and multiple functions, Toet-maru, Dae-cheong, and Nu-maru.

사람의 말을 발성하는 개의 사례 연구 (Case Study of a Dog Vocalizing Human's Words)

  • 견두헌;배명진
    • 한국음향학회지
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    • 제31권4호
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    • pp.235-243
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    • 2012
  • 본 논문은 사람의 언어를 발성하는 개의 사례를 수동성, 능동성으로 구분하여 음원특징과 발성 원인 등을 연구하였다. 사람의 말을 발성하는 기존 사례들을 분석한 결과, 일부 개는 주인 음성의 음향특성을 이해하고 그것을 자신의 발성기관을 통해 일시적인 소리모방이 가능하다. 이것은 언어적 소통의 기능이 없는 수동적 발성 사례이다. 반면 최근 보고된 /엄마/, /누나야/ 발성을 하는 개는 기존 사례들과 확연히 구별되는 특징을 보인다. 해당 개는 상황에 따라 해당 단어를 능동적으로 반복 발성하며, 주인과 기초적 소통과 교감 기능을 수행한다. 개가 사람의 말을 능동적으로 발성할 수 있는 이유는 두뇌가 우수하고 주인과의 친밀도가 매우 높은 점, 해당 발음에 따른 사람들의 적극적인 반응 등이 원인으로 판단된다. 해당 결과는 동물의 발성 가능 소리와, 언어 학습 가능성 연구에 활용될 수 있다.