• Title/Summary/Keyword: NuMA

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Functional Analyses of Centrosomal Proteins, Nek2 and NuMA in Development of Mouse Gametes and Early Embryos

  • Youn, Hong-Hee;Oh, Hwa-Soon;Lee, Kwang-Hee;Son, Chae-Ick;Lee, Sang-Ho
    • Proceedings of the KSAR Conference
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    • 2003.06a
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    • pp.96-96
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    • 2003
  • Nek2 (NIMA-related protein) is a mammalian cell cycle-regulated kinase that involves in chromosome condensation and centrosome regulation and NuMA (nuclear mitotic apparatus protein) is involved in spindle assembly during a cell cycle. The cellular distribution and organization of the centrosomal components is completely unknown during fertilization and embryonic development. We examined distribution of two well-known centrosomal proteins, Nek2 and NuMA in mouse gametes and embryos to get an insight in the reorganization of centrosomal proteins during germ cell development and early fertilization. Spermatogenic cells, gametes, and embryos were analyzed with anti-Nek2 or -NuMA antibodies by immunological assay, RT-PCR, and overexpression through gene transfection. Mitotically or meiotically active spermatogenic cells were intensively stained with these antibodies in both centrosomes and cytoplasm, whereas the oocytes showed different staining patterns depending on the meiotic stages. During maturation, GV, GVBD, and MI stage were clearly stained with NuMA antibody in the nucleus or cytoplasm at MII. Also, Nek2 was detectable in cytoplasm as scattered spots or chromosome associated at MII. In early developmental embryo, NuMA was detected in nucleus of each blastomere, while Nek2 was detected in cytoplasm. In contrast to previously reported results, Nek2 and NuMA were detected in both decondensing head, and the centriole of demembranated and decondensed sperm or whole body of trypsin-treated sperm for Nek2. During meiotic progress in oocytes, transcripts levels were the highest in MI stage and then downregulated in MII. Also, it shows dramatically change in early developmental embryos, firstly, it was increased until 4 cell stage and reduced in 8 cell stage, and finally, transcript levels were upregulated until blastoscyst. This finding suggests that cnetrosomal component may play an important role in reorganizing of functional centrosome during fertilization process and subsequent development.

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Heat transfer characteristics of the heat pipe using simplified heat transfer model (단순 열전달 모델을 이용한 히트파이프의 열전달 성능특성에 관한 연구)

  • Seo, Jae-Hyeong;Bang, Yu-Ma;Seo, Lee-Soo;Lee, Moo-Yeon
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.16 no.1
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    • pp.15-20
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    • 2015
  • The objective of this study was to examine numerically the heat transfer and flow characteristics of the heat pipe with a wick using the simplified heat transfer model to enhance the cooling effects of high heat flux devices and minimizing the energy consumption for electric vehicles. The heat pipe with a wick was analyzed using commercial software with COMSOL and water was used as the working fluid. The velocity and temperature characteristics of the heat pipe were simulated numerically along the heat pipe and the local and average Nusselt numbers were calculated. As a result, the driving force occurred because of the temperature difference between the hot side and the cold side. The heat transfer of the heat pipe occurred from the hot side to the cold side and increased toward the center position. In addition, the average Nusselt numbers were 1.88 for the hot side and 0.1 for the cold side, and the maximum Nusselt number was 4.47 for the hot side and 0.7 for the cold side.

Analytical Study on the Cooking in Eum Sik Bo ("음식보(飮食譜)" 의 조리(調理)에 관한 분석적(分析的) 고찰(考察))

  • Kim, Gwi-Young;Lee, Sung-Woo
    • Journal of the Korean Society of Food Culture
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    • v.3 no.2
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    • pp.135-142
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    • 1988
  • "Eum Sik Bo," the old cook book in Korean, had been kept on microfilm by Hwang, but the condition of copy was not clear so that interpretation of the content was very difficult. The interpreted content was classified and analyzed. The kinds of cooking food recorded in this book were alcohol beverage 12, Side dish 12, Dessert 11, etc. The materials used for the cooking foods cereals, meats, fishes, honey, vegetables, etc. - were various and the cooking methods were described in detail. But the unit of measuring and the used utensils were very poor. The special feature of "Eum Sik Bo" were the fact that red pepper was not used as seasoning and that Nu Ruk was used for Sik He and Ma ji Jub for Nu Ru Mi compared with "Eum Sik Di Mi Bang" and "Zu Bang Moon" According to these points, "Eum Sik Bo" was supposed to be written contemporary or a little later than "Eum Sik Di Mi Bang(1670's)" and "Zu Bang Moon (the end of 17th Century)."

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Crack Tip Creep Deformation Behavior in Transversely Isotropic Materials (횡방향으로 등방성인 재료에서 균열선단 크리프 변형 거동)

  • Ma, Young-Wha;Yoon, Kee-Bong
    • Transactions of the Korean Society of Mechanical Engineers A
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    • v.33 no.12
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    • pp.1455-1463
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    • 2009
  • Theoretical mechanics analysis and finite element simulation were performed to investigate creep deformation behavior at the crack tip of transversely isotropic materials under small scale creep (SCC) conditions. Mechanical behavior of material was assumed as an elastic-$2^{nd}$ creep, which elastic modulus ( E ), Poisson's ratio ( ${\nu}$ ) and creep stress exponent ( n ) were isotropic and creep coefficient was only transversely isotropic. Based on the mechanics analysis for material behavior, a constitutive equation for transversely isotropic creep behavior was formulated and an equivalent creep coefficient was proposed under plain strain conditions. Creep deformation behavior at the crack tip was investigated through the finite element analysis. The results of the finite element analysis showed that creep deformation in transversely isotropic materials is dominant at the rear of the crack-tip. This result was more obvious when a load was applied to principal axis of anisotropy. Based on the results of the mechanics analysis and the finite element simulation, a corrected estimation scheme of the creep zone size was proposed in order to evaluate the creep deformation behavior at the crack tip of transversely isotropic creeping materials.

Users' Perceptions and Attitudes Regarding Use of Korean Traditional Elements in Contemporary Spaces: Focused on the Article Contents of Professional Design Magazines (현대공간의 전통요소에 대한 사용자 인식과 태도: 전문 디자인잡지의 기사 내용을 중심으로)

  • Lee, Min-Ah
    • Journal of the Korean Home Economics Association
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    • v.48 no.7
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    • pp.77-87
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    • 2010
  • This study investigated users' perceptions and attitudes toward Korean traditional elements in contemporary spaces. We collected and analyzed the article contents of two design magazines, from their issues of January 2000 through December 2009, for a total of 87 cases. The results were as follows. First, the important motives for applying traditional elements to current living spaces were individual tastes, preference for a secluded life, and the users' childhood memories. Second, the most frequently used traditional element was traditional structure. Others were traditional spatial compositions and the use of natural materials. The two main purposes for renovating a space or constructing a building were to preserve traditional ambience or to add convenience and practical use to it. Third, the most frequently mentioned advantages of traditional elements were their environment-friendly characteristics, such as connecting to nature and using natural materials. Fourth, the users preferred, in order, Ma-dang, natural environments, doors/windows of various types and multiple functions, Toet-maru, Dae-cheong, and Nu-maru.

Case Study of a Dog Vocalizing Human's Words (사람의 말을 발성하는 개의 사례 연구)

  • Kyon, Doo-Heon;Bae, Myung-Jin
    • The Journal of the Acoustical Society of Korea
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    • v.31 no.4
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    • pp.235-243
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    • 2012
  • This paper studies characteristics and causes of sound, and many others by distinguishing passivity and activity of the cases of a dog vocalizing human's words. As a result of the previous cases of vocalization of human's words, the dog was able to understand characteristics of a host's voice and imitate the sound using his own vocal organs. This is the case of passive vocalization accompanied by temporary voice imitation without a function of communication. On the contrary, as a consequence of the recently reported case in which a dog vocalizes such words as "Um-ma" and "Nu-na-ya," it shows the vocalization pattern clearly distinguished from the prior cases. The given dog repeatedly vocalizes pertaining words in an active manner according to circumstances and plays a role of fundamental communication and interaction with its host. The reason why the dog can vocalize the man's words actively is determined to be that the dog has a high level of intelligence and intimacy with its host, that people react actively to its pertaining pronunciation, and so forth. The following results can be used for the study that investigates animals' sound with vocalization possibility and language learning feasibility.