• Animal cells and systems
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• 제7권4호
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• pp.337-343
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• 2003

Methyl jasmonate (MeJA) is a signal molecule in the activation of defense responses in plants. In this study, we isolated 15 MeJA-inducible genes by subtractive hybridization. These genes encode two myrosinase-binding proteins, five lipase-like proteins, a polygalacturonase inhibitor, a putative chlorophyll-associated protein, a terpene synthase, a dehydroascorbate reductase, an ascorbate oxidase, a cysteine protease, an O-methyltransferase, and an epithiospecifier protein. Northern analysis showed that most of the Chinese cabbage genes are barely expressed in healthy leaves, but are strongly induced by MeJA treatment. We also examined whether these MeJA-inducible genes were activated by ethethon, BTH, and Pseudomonas syringae pv. tomato (Pst), a nonhost pathogen of Chinese cabbage. The results showed that none of the MeJA-inducible genes was strongly induced by ethephon or by BTH. The genes encoding lipase-like proteins and a myrosinase-binding protein were weakly induced by Pst. Other MeJA-inducible genes were not activated at all by the pathogen.

• 한국가구학회지
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• 제20권6호
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• pp.605-611
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• 2009

This paper reports the application of radiocarbon wiggle matching for Korean wooden artifacts such as furniture and Buddhist statues for precise dating. Ten biannual samples of 20 years (AD 1249-1268) for AMS (accelerator mass spectrometry) radiocarbon measurements were prepared from a board of the pedestal for Buddhist statue at Jeongsusa (temple) in Kangwhado, Korea, which was dendrochronologically dated. The average 95.4% confidence interval of radiocarbon dating without wiggle matching was 123 year. When wiggle matching technique was applied, it became 37 year, 3.3 times smaller than that without wiggle matching. The results indicated that wiggle matching technique using the calibration curve for northern hemisphere (IntCal04: International radiocarbon calibration curve announced in 2004) can produce precise dates for Korean wooden artifacts which possess as much as 20 tree rings.

• 대한수의학회지
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• 제38권3호
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• pp.581-588
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• 1998

Eight monoclonal antibodies(MAbs) against bovine coronavirus(BCV) were produced and characterized. Three MAbs(1G9, 4H12, 5C1) specific to the S glycoprotein and two HE glycoprotein-specific MAbs(2A5, 5G4) were found to neutralize the BCV in fluorescence focus neutralization(FFN) test. Two HE-specific MAbs from the neutralizing MAbs inhibited the hemagglutinating activity of the BCV. None of the N protein-specific MAbs(1C1, 5A12, 6H1) neutralized the virus infectivity. Bovine coronavirus and mouse hepatitis virus, which belong to group II coronaviruses, were differentiated from other groups of coronaviruses(porcine transmissible gastroenteritis virus, porcine epidemic diarrhea virus, canine coronavirus) by all MAbs in fluorescence antibody test(FA), but not in FFN test.

• 한국응용곤충학회지
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• 제59권4호
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• pp.353-356
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• 2020

개미사돈아과의 미기록종인 멧개미사돈(Parapyxidicerus carinatus Sawada, 1964)(신칭)을 한국에서 처음으로 보고하며 이들이 속한 한국 미기록족 Bythinoplectini에 대해 함께 보고한다. 털어잡기 방법으로 세 개체의 표본이 경기와 강원도의 물가에서 확보되었다. 정확한 동정을 위해 외형과 진단형질의 사진 그리고 분포지도를 함께 제공한다.

• BMB Reports
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• 제47권6호
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• pp.330-335
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• 2014

Turnip yellow mosaic virus (TYMV) is a spherical plant virus that has a single 6.3 kb positive strand RNA as a genome. In this study, RNA1 sequence of Flock house virus (FHV) was inserted into the TYMV genome to test whether TYMV can accommodate and express another viral entity. In the resulting construct, designated TY-FHV, the FHV RNA1 sequence was expressed as a TYMV subgenomic RNA. Northern analysis of the Nicotiana benthamiana leaves agroinfiltrated with the TY-FHV showed that both genomic and subgenomic FHV RNAs were abundantly produced. This indicates that the FHV RNA1 sequence was correctly expressed and translated to produce a functional FHV replicase. Although these FHV RNAs were not encapsidated, the FHV RNA having a TYMV CP sequence at the 3'-end was efficiently encapsidated. When an eGFP gene was inserted into the B2 ORF of the FHV sequence, a fusion protein of B2-eGFP was produced as expected.

• 한국산업융합학회 논문집
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• 제7권3호
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• pp.281-288
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• 2004

The objective of this study is to propose a methodology of the flood runoff analysis in steep mountainous basins and the analysis basin is the Jasa valley basin in Chungju city Analyzing the spatial pattern of the rainfall in 1994. 6 30~7.1, the seasonal rainy front was tied up in the whole central district, and the rainfall center was moving from the northern Chungbuk province to the northern Kyongbuk province and caused heavy storm. Analyzing the temporal pattern with the Huff method, the 52.5% of the rainfall was concentrated on the 3rd quartile. Rainfall frequency analysis is accomplished by five distribution types; 2-parameter Lognomal, 3-parameter Lognomal, Pearson Type III, Log-Pearson Type III and Extremal Type I distribution Rainfall-runoff analysis in Jasa valley basin was made using HEC-HMS model. Jasa valley basin was divided into 3 sub-basins and the analysis point was 3 points{A, B and C point) With the rainfall data measured by the 10 minutes, the flood runoff also was calculated by as many minutes. SCS CN model, Clark UH model and Muskingum routing model in HEC-HMS model were used to simulate the runoff volume using selected rainfall event.

• 한국생활과학회지
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• 제5권2호
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• pp.173-186
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• 1996

The purpose of this study was to investigate consumers' utilization and satisfaction of the current food labeling system, their awareness of the nutrition labeling and its necessity, and their acceptance of the future enforcement of nutrition labeling. Questionnaires were distributed to 500 adult female consumers in Northern area of Kyonggi-do. Out of 402 questionnaires collected 358(89.1%) were analyzed using SAS computer program. The findings of this study were as follow: First, in the aspects of consumers' utilization and satisfaction of the current food labeling system, although they payed much attention to the food labels when they purchased food goods, the degree of their satisfaction in food labels was low. Among items in food labels they payed more attention to the expiration date, the date of production, and the name of manufacturer. However, the current food labeling system does not offer sufficient and reliable informations to consumers. Second, in the awareness of nutrition labeling and its necessity, majority of consumers appeared not to have seen the nutrition labeling in food products either domestic or imported. However, 93% of respondents answered that nutrition labeling is necessary. Third, in terms of the consumers' acceptance of enforcement of nutrition labeling, they were affirmative in bearing the increased costs, and their expectation to the beneficial effects of enforcement of nutrition labeling was stronger than worrying about the disadvantages or side effects of it.

• 농촌계획
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• 제24권3호
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• pp.137-144
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• 2018

The purpose of this study is to measure the cost-benefit of a road construction project in Vietnam through international development cooperation, which includes private grants and Official Development Assistance(ODA). In developing countries, it is difficult and costly to use data on markets, the CVM(Contingent Valuation Method) to measure the WTP(Willingness-to-Pay) as the benefits of road construction are used. A total of 232 villagers in 16 villages of Lao Cai Province in Northern Vietnam have been surveyed. It is found that the Benefit-Cost Ratio(B/C) in all 3 models are found to be well over 1.0, ranging from 2.8 to 3.64 with the discount rate of 0.06. These BC ratios imply the road construction project in Lao Cai can be justified. Also, it is found that the shortened travel time is the most relevant factor for WTP among direct benefits and the enhanced academic achievement of the children is the most relevant factor among indirect benefits, due to easier and faster access to schools, which in turn increase the school attendance rate. The result of this study can be used to justify other similar projects in Vietnam, even though the benefits may have to be reassessed in other countries.

• 한국작물학회지
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• 제66권3호
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• pp.210-219
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• 2021

최근 지구온난화에 의해 기온이 상승하여 조생종을 주로 재배하는 중북부중산간지대에 중생종과 중만생종의 적응성을 검토하기 위해서 출수생태형별로 이앙시기를 달리해서 조생종 오대, 중생종 청품, 중만생종 삼광을 제천의 농가포장에서 시험한 결과는 다음과 같다. 1. 벼 생육에 영향을 미치는 기상요소 중 평균기온은 2010년대에 8월까지는 가장 높았고, 다른 시기보다 월별로 0.7~0.9℃가 높았으며, 일조시간은 장마, 태풍 등으로 인한 기상이변으로 가장 낮은 수준이었다. 2. 지난 35년간 오대의 지역적응시험 결과 출수기는 점점 빨라지고 있으며, 이앙부터 출수기까지 재배일수가 짧아져 간장과 수수가 감소되었다. 과거보다 등숙률은 최대 10%, 천립중은 2 g 이상 증가되었으나, 품질과 직결되는 출수 후 40일간 평균기온은 많게는 2℃ 이상 증가되었다. 3. 제천지역 안전출수한계기는 출수 후 40일간 적산온도 880℃ 기준으로는 8월 11일, 840℃ 기준으로는 8월 15일로 분석되었다. 4. 이앙시기별 출수생태형에 따라 생육은 비슷하였으나 이앙시기별 최고, 최저 수준을 비교한 등숙률은 조생종 6.5%, 중생종 3.7%, 중만생종 2.5% 높았다. 쌀수량은 조생종 83 kg, 중생종 113 kg, 중만생종 47 kg 많았으며, 완전미율은 조생종 17.8%, 중생종 3.2%, 중만생종 5.0% 높았다. 5. 중북부중산간지에서 안전출수한계기 및 완전미수량을 고려한 이앙시기는 조생종은 6월 15일, 중생종은 6월 5일, 중만생종은 5월 25일이었으며, 본 시험 지역(IV-1)에서도 온난화로 인해 중생종과 중만생종이 안정적으로 재배될 수 있을 것으로 예상된다.

• 대한수의학회지
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• 제31권3호
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• pp.295-302
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• 1991

From 1988 to 1989, 8 strains of canine parvovirus-2(CPV-2) were isolated from the fecal specimens from the dogs that were clinically diagnosed as canine parvoviral enteritis in the veterinary hospitals located in the regions of Taejeon and Chungbuk province. The biological and physicochemical properties for the isolates were studied. Among 62 fecal samples collected from the dogs with enteric diseases, 24(38.7%) showed the haemagglutinating activity to porcine erythrocyte ranging from 16 to 16,384 of HA titers. In cytopathological studies with CRFK cells, intranuclear inclusion bodies were observed in all of eight specimens with the high HA titer over 1,000, of which three specimens showed cytoplasmic inclusions concurrently with the intranuclear inclusion bodies. It was found that the isolates revealed the highest haemagglutinating activity with porcine erythrocytes and the relatively lower haemagglutination titers with the erythrocytes from cat and rabbit. None of erythrocytes from the other animals reacted with the isolates. By the cross-haemagglutination inhibition test for the isolates with the reference viruses and sera, the isolates were evidently identified as the strains of CPV-2. In physicochemical property test, the isolates were stable in lipid solvent, pH and heat treatment at $56^{\circ}C$ for 30 min, and showed the virus particle size less than 25 nm, containing a DNA genome.