• 대한한방내과학회지
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• 제43권4호
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• pp.606-624
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• 2022

Objective: The aim of this study was to compare the effects of different doses of Geumnyeonyijin-tang (GNYJT) water extracts with those of metformin (250 mg/kg) in mild diabetic-obese mice. Methods and Results: The 48 mice were divided into 1 normal pellet diet (NFD) group and 5 high-fat diet (HFD) groups. At the end of 12 weeks of oral administration of metformin (250 mg/kg) or GNYJT water extracts (400, 200, or100 mg/kg), the effects were evaluated. The HFD control mice showed noticeable increases in body weight, adipose tissue density, fat pad weight of the periovarian and abdominal wall, and insulin, blood glucose, and HbA1c levels, with decreases in serum HDL levels. Increases in the periovarian and dorsal abdominal fat pad, regions of steatohepatitis, adipocyte hypertrophy, and hepatocyte hypertrophy were also discovered. The HFD group showed a decline in glucose levels and elevation of hepatic gluconeogenesis, suggesting an HFD-induced AMPK downregulation related to glucose dysregulation, as well as lipid metabolism related to obese insulin-resistant type II diabetes, dyslipidemia, and oxidative stress related diabetic hepatopathy (non-alcoholic fatty liver disease, NAFLD). Conclusion: Assessment of the key parameters for inhibition of diabetes and related complications in HFD-fed diabetic-obese mice demonstrated that GNYJT water extracts have favorable ameliorating effects. The effect of GNYJT was manifested through the stimulation of AMPK upregulation of related hepatic glucose enzyme activities and expression of lipid metabolism-related genes. Therefore, appropriate oral dosages of GNYJT could be promising as a new preventive candidate for controlling diabetes and related complications. Further screening of biologically active compounds, elucidation of detailed mechanisms, and more animal studies are warranted.

• 항공우주시스템공학회지
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• 제16권5호
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• pp.78-85
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• 2022

산악이나 도서 지역으로의 물품수송, 재난지역의 영상정보 획득 및 긴급 구호물품 등을 수송하는데 멀티콥터 형태의 무인기를 활용하고자 하는 수요가 증가하고 있다. 이와 같은 임무를 성공적으로 수행하기 위해서는 비행 조건에 따라 발생하는 하중을 기체 구조물이 안전하게 지지하는 동시에 프롭로터의 진동 및 공탄성 안정성 확보 여부를 확인할 필요가 있다. 본 논문에서는 엔진과 발전기 조합의 하이브리드 동력 시스템이 장착된 탑재중량 40kg급 멀티콥터 무인기의 구조해석 모델 생성과 하중조건에 따른 변형 및 응력 분포 검토과정을 소개하였다. 또한 비행 속도와 기체의 피치각 조건에 따른 프롭로터 시스템의 진동 특성과 공탄성 안정성 해석 결과를 제시하였다. 프롭로터를 통해 발생하는 최대추력 및 정상, 비정상 착륙조건에 따라 기체에 작용하는 착륙하중을 검토하였으며, 구조물의 파손 없이 지지할 수 있음을 확인하였다. 기체의 비행 속도와 프롭로터의 회전속도에 따라 주요 모드별 감쇠 특성이 안정한 영역에 위치함을 확인하였다.

• Wind and Structures
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• 제34권2호
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• pp.173-183
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• 2022

The galloping of iced conductors has long been a severe threat to the safety of overhead transmission lines. Compared with normal transmission lines, the ultra-high-voltage (UHV) transmission lines are more prone to galloping, and the damage caused is more severe. To control the galloping of UHV lines, it is necessary to conduct a comprehensive analysis of galloping characteristics. In this paper, a large-span 1000-kV UHV transmission line in China is taken as a practical example where an 8-bundled conductor with D-shaped icing is adopted. Galerkin method is employed for the time history calculation. For the wind attack angle range of 0°~180°, the galloping amplitudes in vertical, horizontal, and torsional directions are calculated. Furthermore, the vibration frequencies and galloping shapes are analyzed for the most severe conditions. The results show that the wind at 0°~10° attack angles can induce large torsional displacement, and this range of attack angles is also most likely to occur in reality. The galloping with largest amplitudes in all three directions occurs at the attack angle of 170° where the incoming flow is at the non-iced side, due to the strong aerodynamic instability. In addition, with wind speed increasing, galloping modes with higher frequencies appear and make the galloping shape more complex, indicating strong nonlinear behavior. Based on the galloping amplitudes of three directions, the full range of wind attack angles are divided into five galloping regions of different severity levels. The results obtained can promote the understanding of galloping and provide a reference for the anti-galloping design of UHV transmission lines.

• International Journal of Oral Biology
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• 제47권3호
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• pp.41-48
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• 2022

Ulva compressa Linnaeus (UCL) is a green algae seaweed that performs photosynthesis and is used as a food material in some Asian regions including Korea. It is known to be the dominant species in copper ion-contaminated seas, and many studies on copper ion resistant mechanisms have been reported. UCL is known to have an excellent antioxidant effect, but limited information is available regarding its other physiological activities. In this study, we investigated the anticancer activity of 30% prethanol extracts of Ulva compressa Linnaeus (30% PeUCL) and the underlying mechanisms of its activity on human FaDu hypopharyngeal squamous carcinoma cells. The 30% PeUCL extracts suppressed FaDu cell viability without affecting normal cells (L929), as determined by MTT and viability assays. Furthermore, the 30% PeUCL extracts induced apoptosis, as determined by DAPI staining. The 30% PeUCL extracts inhibited colony formation effectively as well as wound-healing of FaDu cells, even at noncytotoxic concentrations. In addition, 30% PeUCL extracts induced apoptosis significantly through proteolytic cleavage of caspase-3, -7, and -9, and poly (ADP-ribose) polymerase, and by downregulation of Bcl-2 and upregulation of Bax in FaDu cells, as determined by Western blot analysis. Collectively, these results suggest that the inhibitory effect of 30% PeUCL extracts on the growth of oral cancer cells, colony formation and wound-healing may be mediated by caspase- and mitochondrial-dependent apoptotic pathways in human FaDu hypopharyngeal squamous carcinoma cells. Therefore, 30% PeUCL extracts can be administered as a natural chemotherapeutic drug for the treatment of human oral cancers.

• 한국응용약물학회:학술대회논문집
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• 한국응용약물학회 1996년도 춘계학술대회
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• pp.212-212
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• 1996

Effects of ginseng total saponin(GTS) on changes in the glutamatergic nervous system induced by AF64A were studied in rats. Rats were pretreated with the infusion of AF64A (3mM) into lateral ventricle and were posttreated with GTS (50mg/kg, j.p) for 1 week. Twenty four hrs after the last administration, rats were sacrified and each brain resions was dissected ; striatum, hippocampus and frontal cortex. At each brain regions, total glutamate and other amino acids levels, [$^3$H]MK801 binding sites and glutamine synthetase activity were measured using HPLC-ECD, ligand binding assay and enzyme activity assay, respectively. The AF64-induced increase in the levels of total glutamate in hippocampus were significantly decreased by the administration of GTS. Furthermore, that compared with saline and GTS was decreased in striatum. The levels of total GABA compared with saline and GTS were declined in frontal cortex. Moreover, the AF64A-induced decrease in the levels of total taurine were significantly increased by the administration of GTS to extents of normal states. The numbers of [$^3$H]MK801 binding sites were differently affected in brain resiojns ; the decrease in hippocampus and no change in both striatum and frontal cortex, Glutamine synthetase activity was significantly increased in hippocampus. In comparision with saline and GTS, that was significantly decreased in striatum These results suggest that GTS may adjust the levels of glutamate, GABA and taurine constantly and may induce increase of glutamine synthetase activity declined.

• Advances in materials Research
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• 제11권2호
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• pp.147-164
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• 2022

This study aims to investigate the influence of scoria aggregate (SA) and silica fume (SF) as a replacement of conventional aggregate and ordinary Portland cement (OPC), respectively. Three types of concrete were prepared namely normal weight concrete (NWC) using limestone aggregate (LSA) and OPC (control specimen), lightweight concrete (LWC) using SA and OPC, and LWC using SA and partial SF (SLWC). The representative workability and compressive strength properties of the developed concrete were evaluated, and the results were correlated with non-destructive ultrasonic pulse velocity and Schmidt hammer tests. The LWC and SLWC yielded compressive strength of around 30 MPa and 33 MPa (i.e., 78-86% of control specimens), respectively. The findings indicate that scoria can be beneficially utilized in the development of structural lightweight concrete. Present renewable sources of aggregate will preserve the natural resources for next generation. The newly produced eco-friendly construction material is intended to break price barriers in all markets and draw attraction of incorporating scoria based light weight construction in Saudi Arabia and GCC countries. Findings of the SWOT analysis indicate that high logistics costs for distributing the aggregates across different regions in Saudi Arabia and clients' resistant to change are among the major obstacles to the commercialized production and utilization of lightweight concrete as green construction material. The findings further revealed that huge scoria deposits in Saudi Arabia, and the potential decrease in density self-weight of structural elements are the major drivers and enablers for promoting the adoption of lightweight concrete as alternative green construction material in the construction sector.

• Structural Engineering and Mechanics
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• 제88권2호
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• pp.117-128
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• 2023

On February 6, 2023, Türkiye woke up with a strong ground motion felt in a wide geography. As a result of the Kahramanmaraş, Pazarcık and Elbistan earthquakes, which took place 9 hours apart, there was great destruction and loss of life. The 2023 Kahramanmaraş earthquakes occurred on active faults known to pose a high seismic hazard, but their effects were devastating. Seismic code spectra were investigated in Hatay, Adıyaman and Kahramanmaraş where destruction is high. The study mainly focuses on the investigation of ground motion parameters of 6 February Kahramanmaraş earthquakes and the correlation between ground motion parameters. In addition, earthquakes greater than Mw 5.0 that occurred in Türkiye were compared with certain seismic parameters. As in the strong ground motion studies, seismic energy parameters such as Arias intensity, characteristic intensity, cumulative absolute velocity and specific energy density were determined, especially considering the duration content of the earthquake. Based on the study, it was concluded that the structures were overloaded far beyond their normal design levels. This, coupled with significant vertical seismic components, is a contributing factor to the collapse of many buildings in the area. In the evaluation made on Arias intensity, much more energy (approximately ten times) emerged in Kahramanmaraş earthquakes compared to other Türkiye earthquakes. No good correlation was found between moment magnitude and peak ground accelerations, peak ground velocities, Arias intensities and ground motion durations in Türkiye earthquakes. Both high seismic components and long ground motion durations caused intense energy to be transferred to the structures. No strong correlation was found between ground motion durations and other seismic parameters. There is a strong positive correlation between PGA and seismic energy parameter AI. Kahramanmaraş earthquakes revealed that changes should be made in the Turkish seismic code to predict higher spectral acceleration values, especially in earthquake-prone regions in Türkiye.

• 한국전문물리치료학회지
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• 제31권1호
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• pp.40-47
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• 2024

Background: Single-leg squat (SLS)s are commonly used as assessment tool and closed kinetic exercises are useful for assessing performance of the lower extremities. Pronated feet are associated with foot pressure distribution (FPD) during daily activities. Objects: To compare the FPD during SLSs between groups with pronated and normal feet. Methods: This cross-sectional study included 30 participants (15 each in the pronated foot and control groups) are recruited in this study. The foot posture index was used to distinguish between the pronated foot and control groups. The Zebris FDM (Zebris Medical GmbH) stance analysis system was used to measure the FPD on the dominant side during a SLS, which was divided into three phases. A two-way mixed-model ANOVA was used to identify significant differences in FPD between and within the two groups. Results: In the hallux, the results of the two-way mixed-model ANOVAs revealed a significant difference between the group and across different phases (p < 0.05). The hallux, and central forefoot were significantly different between the group (p < 0.05). Moreover, significant differences across different phases were observed in the hallux, medial forefoot, central forefoot, lateral forefoot, and rearfoot (p < 0.05). The post hoc t-tests were conducted for the hallux and forefoot central regions. In participants with pronated foot, the mean pressure was significantly greater in hallux and significantly lower, in the central forefoot during the descent and holding phases. Conclusion: SLSs are widely used as screening tests and exercises. These findings suggest that individuals with pronated feet should be cautious to avoid excessive pressure on the hallux during the descent-to-hold phase of a SLS.

• Animal Bioscience
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• 제37권8호
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• pp.1317-1332
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• 2024

Objective: Rare study of the non-coding and regulatory regions of the genome limits our ability to decode the mechanisms of fatty liver hemorrhage syndrome (FLHS) in chickens. Methods: Herein, we constructed the high-fat diet-induced FLHS chicken model to investigate the genome-wide active enhancers and transcriptome by H3K27ac target chromatin immunoprecipitation sequencing (ChIP-seq) and RNA sequencing (RNA-Seq) profiles of normal and FLHS liver tissues. Concurrently, an integrative analysis combining ChIP-seq with RNA-Seq and a comparative analysis with chicken FLHS, rat non-alcoholic fatty liver disease (NAFLD) and human NAFLD at the transcriptome level revealed the enhancer and super enhancer target genes and conservative genes involved in metabolic processes. Results: In total, 56 and 199 peak-genes were identified in upregulated peak-genes positively regulated by H3K27ac (Cor (peak-gene correlation) ≥0.5 and log2(FoldChange) ≥1) (PP) and downregulated peak-genes positively regulated by H3K27ac (Cor (peak-gene correlation) ≥0.5 and log2(FoldChange)≤-1) (PN), respectively; then we screened key regulatory targets mainly distributing in lipid metabolism (PCK1, APOA4, APOA1, INHBE) and apoptosis (KIT, NTRK2) together with MAPK and PPAR signaling pathway in FLHS. Intriguingly, PCK1 was also significantly covered in up-regulated super-enhancers (SEs), which further implied the vital role of PCK1 during the development of FLHS. Conclusion: Together, our studies have identified potential therapeutic biomarkers of PCK1 and elucidated novel insights into the pathogenesis of FLHS, especially for the epigenetic perspective.

• The Korean Journal of Physiology and Pharmacology
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• 제28권1호
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• pp.93-103
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• 2024

Satellite glial cells (SGCs), a major type of glial cell in the autonomic ganglia, closely envelop the cell body and even the synaptic regions of a single neuron with a very narrow gap. This structurally unique organization suggests that autonomic neurons and SGCs may communicate reciprocally. Glial Ca²⁺ signaling is critical for controlling neural activity. Here, for the first time we identified the machinery of store-operated Ca²⁺ entry (SOCE) which is critical for cellular Ca²⁺ homeostasis in rat sympathetic ganglia under normal and pathological states. Quantitative realtime PCR and immunostaining analyses showed that Orai1 and stromal interaction molecules 1 (STIM1) proteins are the primary components of SOCE machinery in the sympathetic ganglia. When the internal Ca²⁺ stores were depleted in the absence of extracellular Ca²⁺, the number of plasmalemmal Orai1 puncta was increased in neurons and SGCs, suggesting activation of the Ca²⁺ entry channels. Intracellular Ca²⁺ imaging revealed that SOCE was present in SGCs and neurons; however, the magnitude of SOCE was much larger in the SGCs than in the neurons. The SOCE was significantly suppressed by GSK7975A, a selective Orai1 blocker, and Pyr6, a SOCE blocker. Lipopolysaccharide (LPS) upregulated the glial fibrillary acidic protein and Toll-like receptor 4 in the sympathetic ganglia. Importantly, LPS attenuated SOCE via downregulating Orai1 and STIM1 expression. In conclusion, sympathetic SGCs functionally express the SOCE machinery, which is indispensable for intracellular Ca²⁺ signaling. The SOCE is highly susceptible to inflammation, which may affect sympathetic neuronal activity and thereby autonomic output.