• Korean Journal of Agricultural Science
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• v.45 no.1
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• pp.28-37
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• 2018

This study was done to develop environmental risk assessments and a biosafety guide for insect-resistant genetically modified rice at a LMO (Living Modified Organism) isolation field. In the LMO quarantine area of Kyungpook National University, the species diversities and population densities of non-target insects found on insect-resistant genetically modified rice (Bt-9) resistant to Cnaphalocrocis medinalis and on non-GM rices (Dongjin and Ilmi) were investigated. The Bt-9 event was therefore evaluated under field conditions to detect possible impacts on the above ground insects and spiders. The study compared transgenic rice and two non-GM reference rices, Ilmi and Dongjin, at Gunwi in Southern Korea in 2016. Each rice was grown on three $18m^2$ plots with a randomized block design. A total of 4,243 individuals from 43 families and 9 orders were collected from the LMO isolation field. In the three types of rice fields, a total of 1,467 individuals from the insect-resistant genetically modified rice (Bt-9), 1,423 individuals from the Ilmi, and 1,353 individuals from the Dongjin were collected, respectively. There was no difference between the population densities of the non-target insect pests, natural enemies and other insects on the insect-resistant genetically modified rice (Bt-9) and non-GM rices. These results provide the diversity and population density of non-target insects for an environment risk assessment survey on insect-resistant genetically modified rice and could be used as a guideline to make a biosafety assessment method for genetically modified crops.

• Journal of Korean Society on Water Environment
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• v.37 no.3
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• pp.175-189
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• 2021

In this study, 48 streams in the Sapgyo Watershed were selected, and the Load Duration Curves (LDC) were drawn up for each stream using water quality and flow monitoring over the last three years (2018-2020), and it was evaluated whether the target water quality was achieved for each flow section. As a result of evaluating whether or not the target water quality exceeded according to the LDC, it was found that 22 rivers exceeded the target water quality. Five rivers exceeded the target water quality due to point pollutant sources, 13 rivers exceeded the target water quality due to non-point pollutant sources, and 4 rivers exceeded the target water quality due to both point and non-point pollutant sources. Among the rivers that exceeded the target water quality due to point pollutant sources, which included domestic sewage of the untreated population, there is a need to reduce the influx of polluted loads by the untreated population. The use of eco-friendly fertilizers is recommended for rivers with a relatively high farmland ratio among rivers exceeding the target water quality due to non-point pollutant sources, and installation of boiling point reduction facilities that can reduce the amount of polluted load introduced during rainfall or manage water shores. In rivers with a large number of livestock breeding heads, the livestock houses located in these rivers need to be preferentially transferred to livestock manure treatment plants. Due to the high ratio of land area because of urbanization, initial rainwater treatment facilities are required to reduce the amount of pollutant load flowing into the river through the impermeable layer during rainfall.

• Molecules and Cells
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• v.27 no.6
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• pp.689-695
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• 2009

Chemically synthesized small interfering RNAs (siRNAs) can specifically knock-down expression of target genes via RNA interference (RNAi) pathway. To date, the length of synthetic siRNA duplex has been strictly maintained less than 30 bp, because an early study suggested that double-stranded RNAs (dsRNAs) longer than 30 bp could not trigger specific gene silencing due to the induction of non-specific antiviral interferon responses. Contrary to the current belief, here we show that synthetic dsRNA as long as 38 bp can result in specific target gene silencing without non-specific antiviral responses. Using this longer duplex structure, we have generated dsRNAs, which can simultaneously knock-down expression of two target genes (termed as dual-target siRNAs or dsiRNAs). Our results thus demonstrate the structural flexibility of gene silencing siRNAs, and provide a starting point to construct multifunctional RNA structures. The dsiRNAs could be utilized to develop a novel therapeutic gene silencing strategy against diseases with multiple gene alternations such as viral infection and cancer.

• The Journal of the Acoustical Society of Korea
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• v.43 no.2
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• pp.225-233
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• 2024

Active sonar transmits sound waves to detect covertly maneuvering underwater objects and detects the signals reflected back from the target. However, in addition to the target's echo, the active sonar's received signal is mixed with seafloor, sea surface reverberation, biological noise, and other noise, making target recognition difficult. Conventional techniques for detecting signals above a threshold not only cause false detections or miss targets depending on the set threshold, but also have the problem of having to set an appropriate threshold for various underwater environments. To overcome this, research has been conducted on automatic calculation of threshold values through techniques such as Constant False Alarm Rate (CFAR) and application of advanced tracking filters and association techniques, but there are limitations in environments where a significant number of detections occur. As deep learning technology has recently developed, efforts have been made to apply it in the field of underwater target detection, but it is very difficult to acquire active sonar data for discriminator learning, so not only is the data rare, but there are only a very small number of targets and a relatively large number of non-targets. There are difficulties due to the imbalance of data. In this paper, the image of the energy distribution of the detection signal is used, and a classifier is learned in a way that takes into account the imbalance of the data to distinguish between targets and non-targets and added to the existing technique. Through the proposed technique, target misclassification was minimized and non-targets were eliminated, making target recognition easier for active sonar operators. And the effectiveness of the proposed technique was verified through sea experiment data obtained in the East Sea.

• BMB Reports
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• v.50 no.6
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• pp.329-334
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• 2017

Protein tyrosine phosphatases (PTPs) play crucial roles in signal transduction and their functional alteration has been detected in many diseases. PTP inhibitors have been developed as therapeutic drugs for diseases that are related to the activity of PTPs. In this study, PTP inhibitor XIX, an inhibitor of CD45 and PTEN, was investigated whether it inhibits other PTPs. Protein tyrosine phosphatase non-receptor type 2 (PTPN2) was selectively inhibited by the inhibitor in a competitive manner. Drug affinity responsive target stability (DARTS) analysis showed that the inhibitor induces conformational changes in PTPN2. Phosphorylation levels of signal transducer and activator of transcription 3 (STAT3) at Tyr-705, a crucial site for STAT3 activation and target site of PTPN2, decreased upon exposure to the inhibitor. Our results suggest that PTP inhibitor XIX might be considered as an effective regulator of PTPN2 for treating diseases related to PTPN2.

• Proceedings of the Safety Management and Science Conference
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• 2011.04a
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• pp.635-643
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• 2011

The purpose of this study was to examine the effects of goal-setting on Eco-driving behaviors. An additional purpose was to examine whether setting goals on target behaviors has spread effects on non-target Eco-driving behaviors. Three office workers who drive regularly participated in this study. An AB multiple baseline design was adopted. After baseline (A), participants set goals on two or three target behaviors(e.g., over speeding, put the gears in neutral, extreme acceleration) (B). Results showed that goal-setting was effective in increasing the targeted Eco-driving behaviors. In Addition, most of the non-target Eco-driving behaviors(e.g., extreme break, extreme acceleration) increased.

• Journal of the Korean Society of Safety
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• v.26 no.6
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• pp.124-129
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• 2011

The purpose of this study was to examine the effects of goal-setting on Eco-driving behaviors. An additional purpose was to examine whether setting goals on target behaviors had spread effects on non-target eco-driving behaviors. Three office workers who drive regularly participated in this study. An AB multiple baseline design was adopted. After baseline (A), participants set goals on two or three target behaviors(e.g., over speeding, put the gears in neutral, extreme acceleration) (B). Results showed that goal-setting was effective in increasing the targeted eco-driving behaviors. In addition, most of the non-target eco-driving behaviors(e.g., extreme break, extreme acceleration) increased.

• Journal of IKEEE
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• v.20 no.1
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• pp.1-8
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• 2016

In this paper, we propose an effective time-of-arrival (TOA)-based localization method with adaptive bias computation in indoor environments. The goal of the localization is to estimate an accurate target's location in wireless localization system. However, in indoor environments, non-line-of-sight (NLOS) errors block the signal propagation between target device and base station. The NLOS errors have significant effects on ranging between two devices for wireless localization. In TOA-based localization, finding the target's location inside the overlapped area in the TOA-circles is difficult. We present an effective localization method using compensated distance with adaptive bias computation. The proposed method is possible for the target's location to estimate an accurate location in the overlapped area using the measured distances with subtracted adaptive bias. Through localization experiments in indoor environments, estimation error is reduced comparing to the conventional localization methods.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 2006.06a
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• pp.402-403
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• 2006

This research aims to develop the multiple channel electrochemical DNA chip using microfabrication technology. At first, we fabricated a high integration type DNA chip array by lithography technology. Several probe DNAs consisting of thiol group at their 5-end were immobilized on the gold electrodes. Then target DNAs were hybridized and reacted. Cyclic voltammetry showed a difference between target DNA and control DNA in the anodic peak current values. Therefore, it is able to detect a plural genes electrochemically after immobilization of a plural probe DNA and hybridization of non-labeling target DNA on the electrodes simultaneously. It suggested that this DNA chip could recognize the sequence specific genes.

• KSBB Journal
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• v.10 no.4
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• pp.428-434
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• 1995

Target-sensitive(TG-S) liposomes, which have the antibodies coupled on the surface of liposome and can release their entrapped contents by the binding of antibodies with the specigic target cells, were prepared and employed to study the release of calcein and the selective delivery of an anticancer agent, doxorubicin(DOX). The monoclonal antibody, Y3, used for the preparation of the TG-S liposome was one against major histocompatibility complex class 1 of mouse(MHCI, H-2Kｂtype) and the target cells were EL-4 and RMA, which have the MHC1, H-2Kbtype on their membrane surfacem. The release of calcein from TG-S liposome occurred when the target cells were contacted with liposomes and it was proportionally increased with the rise of binding capacity of antibody coupled on the surface of liposome to the target cells. The experimental results of drug delivery were similar to the cases of calcein release. The viability of specific target cell, EL-4 with liposomal DOX was not so different from that with the free DOX, while for the non-specific target cell, Yacl(H-2Kf), the cell viability with Iiposomal DOX was much higher than that with free DOX. This shows the fact that the liposomal DOX can be efficiently delivered to the specific target cells, while it was not the case for the non-specific target cells. And the drug delivery was lnhibited when the free antibody of Y3 was added in the contact process between EL-4 and TG-S liposomes, which means the drug delivery occurred mainly by the destabilization of TG-S liposomes. From these results, we could conclude that the selective drug delivery to specific target cell using the TG-S liposome would be feasible.