• 문화기술의 융합
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• 제8권6호
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• pp.531-536
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• 2022

전 세계적으로 국가경쟁력, 효용성, 중요성 등의 이유로 메가시티가 속속 등장하고 있으며, 점차 각 국가의 중심지로 자리를 잡아가고 있다. 또한, 메가시티는 현존, 잠재적, 초국가, 비군사 등 다양한 위협에 노출될 수밖에 없다. 특히, 최근 우크라이나-러시아 전쟁에서 볼 수 있듯이 국가기반체계는 적대 및 위협세력의 주요 목표가 되고 있다. 하지만 메가시티의 막대한 규모, 다양한 위협의 양상, 한정된 자원 등을 고려할 때 모든 국가기반체계를 완벽하게 방호하는 것은 현실적으로 어렵다. 따라서 여러 국가기반체계 중 핵심노드를 식별하여 중점적으로 방호하는 접근이 필요하다. 핵심노드는 다양한 국가기반시설이 연결된 국가기반체계의 중요도가 매우 높면서도 동시에 급소에 해당한다. 이를 위해 본 연구에서는 핵심노드를 논리적·체계적으로 식별하기 위해 네트워크 이론인 노드 중심성(Centrality) 분석방법과 군사이론인 중심(Center of Gravity) 분석방법을 제시하였다.

• 의료ㆍ복지 건축 : 한국의료복지건축학회 논문집
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• 제28권1호
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• pp.41-51
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• 2022

Purpose: The purpose of this study is to compare variant analysis results regarding overcrowded spaces and suggest a space analysis model for women's hospitals at the outpatient clinic department, central treatment department, and inpatient ward considering evacuation capability of newborns unable to perform automotive evacuation, pregnant patients and maternal patients with limited mobility. Methods: Firstly, precedent studies on women's hospitals and evacuation in hospitals were reviewed. Secondly, the analysis conditions and necessary set values for simulation were designed after analyzing the design documents of the research target hospital. Thirdly, evacuation simulation was conducted by adjusting the variables related to the evacuation behavior of inpatients and newborns. Fourthly, based on the derived results, overcrowded spaces were compared and analyzed according to evacuation behavior. Result: The study results are as follows: (1) It is necessary to check the main escape routes for occupants and to expand and adjust the size of mainly used entrances. (2) It is necessary to widen the stairs' width to alleviate overcrowding caused by patients with non-autonomous walking. (3) Due to overcrowding of the main escape route, it is necessary to identify the bypass route and adjust the width of the corridor. (4) It is necessary to plan an evacuation route for newborns to escape from the main escape route for occupants. Implications: The study result is expected to be used as primary data for research that considers the evacuation behavior of newborns and pregnant women in women's hospitals.

• 한국환경성돌연변이발암원학회지
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• 제23권2호
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• pp.56-62
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• 2003

The liver progenitor cells could form a potential target cell population fore both tumor-initiating and -promoting chemicals. Induction of drug-metabolizing and antioxidant enzymes, including AhR-dependent CYP1A1, NQO-1 and AKR1C9, was detected in the rat liver epithelial WB-F344 "stem-like" cells. Additionally, WB-F344 cells express a functional, wild-type form of p53 protein, a biomarker of genotoxic events, and connexin 43, a basic structural unit of gap junctions forming an important type of intercellular communication. In this cellular model, two complementary assays have been established for detection of the modes of action associated with tumor promotion: inhibition of gap junctional intercellular communication (GJIC) and proliferative activity in confluent cells. We found that the PAHs and PCBs, which are AhR agonists, released WB-F344 cells from contact inhibition, increasing both DNA synthesis and cell numbers. Genotoxic effects of some PAHs that lead to apoptosis and cell cycle delay might interfere with the proliferative activity of PAHs. Contrary to that, the nongenotoxic low-molecular-weight PAHs and non-dioxin-like PCB congeners, abundant in the environment, did not significantly affect cell cycle and cell proliferation; however both groups of compounds inhibited GJIC in WB-F344 cells. The release from contact inhibiton by a mechanism that possibly involves the AhR activation, inhibition of GJIC and genotoxic events induced by environmental contaminants are three important modes of action that could play an important role in carcinogenic effects of toxic compounds. The relative potencies to inhibit GJIC, to induce AhR-mediated activity, and to release cells from contact inhibition were determined for a large series of PAHs and PCBs and their metabolites. In vitro bioassays based on detection of events on cellular level (deregulation of GJIC and/or proliferation) or determination of receptor-mediated activities in both ?$stem-like^{\circ}{\times}$ and hepatocyte-like liver cellular models are valuable tools for detection of modes of action of polyaromatic hydrocarbons. They may serve, together with concentration data, as a first step in their risk assessment.

• 한국축산식품학회지
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• 제43권1호
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• pp.73-84
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• 2023

Campylobacteriosis is a common cause of gastrointestinal disease. In this study, we suggest a general strategy of applying gold nanoparticles (AuNPs) in colorimetric biosensors to detect Campylobacter in chicken carcass. Polymerase chain reaction (PCR) was utilized for the amplification of the target genes, and the thiolated PCR products were collected. Following the blending of colloid AuNPs with PCR products, the thiol bound to the surface of AuNPs, forming AuNP-PCR products. The PCR products had a sufficient negative charge, which enabled AuNPs to maintain a dispersed formation under electrostatic repulsion. This platform presented a color change as AuNPs aggregate. It did not need additional time and optimization of pH for PCR amplicons to adhere to the AuNPs. The specificity of AuNPs of modified primer pairs for mapA from Campylobacter jejuni and ceuE from Campylobacter coli was activated perfectly (C. jejuni, p-value: 0.0085; C. coli, p-value: 0.0239) when compared to Salmonella Enteritidis and Escherichia coli as non-Campylobacter species. Likewise, C. jejuni was successfully detected from artificially contaminated chicken carcass samples. According to the sensitivity test, at least 15 ng/μL of Campylobacter PCR products or 1×10³ CFU/mL of cells in the broth was needed for the detection using the optical method.

• 농촌계획
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• 제29권3호
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• pp.53-67
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• 2023

In this study, the problem of the idleness of public buildings in rural areas is seriously emerging, but there are no clear standards for assessing the utilization of these buildings. Therefore, this study endeavors to investigate the actual state of idleness of buildings created by rural area development projects based on the criteria for each type. As part of this study, rural facilities were selected in two locations in Sangju City and Miryang City among the target areas of the Rural Agreement. Utilization evaluation criteria were developed to identify the conditions of underutilization. In order to determine the utilization pattern for each facility type, an in-depth interview with the operator and an inspection of the facility were conducted. Based on the analysis, the degree of utilization (e.g., low use, non-use, etc.) was different for each facility type, and among the four facility types (rural tourism, culture and welfare, exercise and recreation, income-based), rural tourism was rated as the least efficient. It has been found that the operating entity's capacity has a significant influence on the utilization of facilities. Socioeconomic factors, such as a decrease in utilization rates, are greatly influenced by the operating entity's capacity. Therefore, support from local governments as well as the national level is required to recycle idle facilities. Lastly, this study suggests the need for different standards for utilization, depending on the type of facility. This moves beyond checking the degree of idleness by the same standard that was previously implemented.

• 한국동물위생학회지
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• 제46권3호
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• pp.193-202
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• 2023

Porcine epidemic diarrhea is an infectious intestinal disease caused by the porcine epidemic diarrhea virus (PEDV). Especially, when suckling piglets are infected, the mortality rate is close to 100%. PEDV is classified into G1 and G2 types based on genetic differences. The G2 type PEDV outbreak in the United States in 2013 was highly pathogenic and contagious, and it has spread worldwide and caused continuous economic losses. Most commercial vaccines used are G1 type vaccines, and existing vaccines do not fully protect piglets due to genetic differences. In this study, we evaluated the safety of the newly developed G2 type attenuated HSGP vaccine strain by inoculating it into piglets and testing whether the vaccine virus spreads to the non-vaccinated, negative pigs and whether the vaccine reverts to its virulence during serial passage experiments. Each experiment lasted for 7 days for each passage, and fecal viral titers, clinical symptoms, and weight gain were measured daily. After the experiment, necropsy was performed to measure intestinal virus titer and pathological evaluation. As a result of the first passage, no transmission of the vaccine virus to negative pigs co-housed with vaccinated pigs was observed. In addition, after four consecutive passage experiments, the clinical symptoms and small intestine lesions were gradually alleviated, and no virus was detected in the feces in the fourth passage experiment. Therefore, it was concluded that the vaccine was safe without virulence reversion in accordance with the guidelines of the current licensing authority. However, further studies are needed on the genetic changes and biological characteristics of the mutant virus that occur during successive passages of the attenuated vaccine since the replication and clinical symptoms of the virus increased until the third passage during successive passages of the vaccine virus. Based on this study, it was concluded that virulence reversion and safety evaluation of attenuated vaccines through serial passage in target animals can be useful to evaluate the safety of attenuated viruses.

• Korean Journal of Acupuncture
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• 제40권2호
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• pp.33-43
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• 2023

Objectives : Floating acupuncture (FA) is a kind of newly developed acupuncture technique that contains its own apparatus. The technique has unique points that the body of the acupuncture needle stays intradermal space and manipulation is performed by shaking the needle horizontally; after manipulation, part of the needle remained in the intradermal space for 2~3 days. FA is not a common acupuncture methodology while various clinical study claims its efficacy on musculoskeletal disorders. In this study, the authors aimed to enhance the use of FA by comparing related theories. Methods : The authors reviewed classics, books, and articles related to FA, Meridian Muscle (MM), Myofascial Pain Syndrome (MPS), and Wrist-Ankle Acupuncture (WAA), and compared its characteristics by related theory, related symptoms, apparatus, and performing procedures. Results : FA was related and had various common parts with MM, MPS, and WAA, however, there were unique parts in the manipulation, apparatus, and stimulation location. FA is based on MM and MPS which pointing 'ashi points' or 'trigger points' as a treating target while FA does not stimulate the points directly. FA also targets subcutaneous space by inserting the needle horizontally as WAA does, while FA remains the needle handle part in the subcutaneous area for 2~3 days for more stimulation. Conclusions : FA has a unique manipulation procedure and potential benefit for musculoskeletal disorders despite the crude theological base written by Fu himself. Thus, developing a new explanation and patient-friendly methodology/apparatus is required for further down-to-earth studies.

• 한국항공운항학회지
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• 제31권2호
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• pp.89-94
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• 2023

Recently, domestic small airport development plans are being promoted centering on islands, and among them, Baengnyeong Island Airport is located very close to North Korea due to its geographical characteristics. In particular, since it is located in the P-518, which is prohibited airspace, it is essential to configure the arrival/departure flight procedure route similarly to the current ship in terms of securing flight safety. Therefore, in this study, a plan for airport location and facility scale was proposed by focusing on the runway operated for the purpose of aircraft operation through consultations with the Ministry of National Defense. It was proposed to meet the standards of the Airport Facility Act as an airport dedicated to domestic flights for airside facilities such as runway length and direction, target aircraft, landing pad, apron, runway and taxiway width appropriate for the topographical characteristics of Baengnyeong Island. As a result, the flight procedure is generally established as a two-way visual flight, but the 10-way arrival/departure procedure was planned to be changed to an instrument (non-precision) according to the specificity of the region. In addition, a flight procedure was established and presented for safe and systematic operation by reflecting the changes in airport facility size (landing pad, runway end safety area, etc.) following the change in instrument flight procedure in the visual flight procedure.

• The Plant Pathology Journal
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• 제39권4호
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• pp.309-318
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• 2023

Huanglongbing (HLB) is one of the most destructive diseases in citrus, which imperils the sustainability of citriculture worldwide. The presumed causal agent of HLB, 'Candidatus Liberibacter asiaticus' (CLas) is a non-culturable phloem-limited α-proteobacterium transmitted by Asian citrus psyllids (ACP, Diaphorina citri Kuwayama). A widely adopted method for HLB diagnosis is based on quantitative real-time polymerase chain reaction (qPCR). Although HLB diagnostic qPCR provides high sensitivity and good reproducibility, it is limited by time-consuming DNA preparation from plant tissue or ACP and the requirement of proper lab instruments including a thermal cycler to conduct qPCR. In an attempt to develop a quick assay that can be deployed in the field for CLas detection, we developed a real-time loop-mediated isothermal amplification (rt-LAMP) assay by targeting the CLas five copy nrdB gene. The rt-LAMP assay using various plant sample types and psyllids successfully detected the nrdB target as low as ~2.6 Log10 copies. Although the rt-LAMP assay was less sensitive than laboratory-based qPCR (detection limit ~10 copies), the data obtained with citrus leaf and bark and ACP showed that the rt-LAMP assay has >96% CLas detection rate, compared to that of laboratory-based qPCR. However, the CLas detection rate in fibrous roots was significantly decreased compared to qPCR due to low CLas titer in some root DNA sample. We also demonstrated that the rt-LAMP assay can be used with a crude leaf DNA extract which is fully deployable in the field for quick and reliable HLB screening.

• Design & Manufacturing
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• 제16권2호
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• pp.33-38
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• 2022

Currently, Korea is an aging society, and it is expected to enter a super-aging society in about 4 years. Accordingly, many X-ray technologies are being developed. In X-rays, 99% of X-rays are converted into heat energy and 1% into light energy (X-rays). 99% of the thermal energy raises the temperature of the anode and its surroundings, and the cooling system is an important factor as overheating can affect the deterioration of X-ray quality and shortened lifespan. There is a method of forced air cooling using natural convection. Therefore, in this study, when X-rays were taken 5 times, Flow analysis was performed on heat removal according to temperature rise and cooling time for the heat generated at the anode of the X-ray tube (input power 60kW, 75kW, 90kW). Based on one-shot, the most rapid temperature rise section increased by more than 57% to 0.03 seconds, A constant temperature rises from 0.03 seconds to 0.1 seconds, It is judged that the temperature rises by about 8.2% or more at one time. After one-shot cooling, the cooling drops sharply from about 60% to 0.03 seconds, It is judged that the temperature has cooled by more than 86% compared to the temperature before shooting. One-shot is cooled by more than 86% with cooling time after 0.1 seconds, As the input power of the anode increases, the cooling temperature gradually increases. Since the tungsten of the anode target inside the X-ray tube may be damaged by thermal shock caused by a rapid temperature rise, an improvement method for removing thermal energy is required when using a high-input power supply.