• Journal of Internet Computing and Services
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• v.10 no.4
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• pp.25-33
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• 2009

In the paper, we proposed a new system to retrieve 3D wrinkle data based on stereo images. Usually, 3D reconstruction based on stereo images or video is very popular and it is the research focus, which has been applied for culture heritage, building and other scene. The target is object measurement, the scene depth calculation and 3D data obtained. There are several challenges in our research. First, it is hard to take the full information wrinkle images by cameras because of light influence, skin with non-rigid object and camera performance. We design a particular computer vision system to take winkle images with a long length camera lens. Second, it is difficult to get the dense stereo data because of the hard skin texture image segmentation and corner detection. We focus on semi-dense stereo matching algorithm for the wrinkle depth. Compared with the 3D scanner, our system is much cheaper and compared with the physical modeling based method, our system is more flexible with high performance.

• Molecules and Cells
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• v.39 no.2
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• pp.119-128
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• 2016

Angelica polymorpha Maxim root extract (APRE) is a popular herbal medicine used for treating stomachache, abdominal pain, stomach ulcers, and rheumatism; however the effect of APRE on cancer cells has not yet been explored. Here, we examined APRE cytotoxicity seen on target neuroblastoma cells (NB) using cell viability assays, DAPI visualization of fragmented DNA, and Western blotting analysis of candidate signaling pathways involved in proliferation and apoptosis. We demonstrated that APRE reduced cell viability in NB to a greater extent than in fibroblast cells. In addition, we found that APRE could inhibit the three classes of MAPK proteins and could also down-regulate the PI3K/AKT/GSK-$3{\beta}$ activity all being relevant for proliferation and survival. APRE could also up-regulate Bax expression and down-regulate Bcl-2 and Mcl-1. With APRE treatment, depolarization of mitochondria membrane potential and activation of caspase-3 was demonstrated in the SH-SY5Y cells. We could not found increased activity of death receptor and caspase-8 as markers of the extrinsic apoptosis pathway for the APRE treated cells. In presence of a caspase-3 siRNA and a pan-caspase inhibitor, APRE could not reduce the viability of NB cells to a significant degree. So we predicted that with APRE, the intrinsic pathway was solely responsible for inducing apoptosis as we also showed that the non-caspase autophagy pathway or ER stress-ROS mediated pathways were not involved. These findings demonstrate that an intrinsic mitochondria-mediated apoptosis pathway mediates the apoptotic effects of APRE on SH-SY5Y cells, and that APRE shows promise as a novel agent for neuroblastoma therapy.

• Biomolecules & Therapeutics
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• v.23 no.5
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• pp.400-406
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• 2015

MicroRNAs (miRNAs) are a family of non-coding RNA that are able to adjust the expression of many proteins, including ATP-binding cassette transporter and organic cation transporter. We sought to evaluate the effect of miR-511 on the regulation of OATP1B1 expression by free fatty acids. When using free fatty acids to stimulate Chang liver cells, we found that the expression of miR-511 increased significantly while the expression of OATP1B1 decreased. We also proved that SLCO1B1 is the target gene of miR-511 with a bioinformatics analysis and using the dual luciferase reporter assay. Furthermore, the expressions of SLCO1B1 and OATP1B1 decreased if transfecting Chang liver cells with miR-511, but did not increase when transfecting the inhibitors of miR-511 into steatosis cells. Our study indicates that miR-511 may play an important role in the regulation of OATP1B1 expression by free fatty acids.

• Biomolecules & Therapeutics
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• v.24 no.1
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• pp.19-24
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• 2016

Acute myocardial infarction (AMI) remains a leading cause of morbidity and mortality worldwide. The exploration of new biomarkers with high sensitivity and specificity for early diagnosis of AMI therefore becomes one of the primary task. In the current study, we aim to detect whether there is any heart specific long noncoding RNA (lncRNA) releasing into the circulation during AMI, and explore its function in the neonatal rat cardiac myocytes injury induced by $H_2O_2$. Our results revealed that the cardiac-specific lncRNA MHRT (Myosin Heavy Chain Associated RNA Transcripts) was significantly elevated in the blood from AMI patients compared with the healthy control ($^*p<0.05$). Using an in vitro neonatal rat cardiac myocytes injury model, we demonstrated that lncRNA MHRT was upregulated in the cardiac myocytes after treatment with hydrogen peroxide ($H_2O_2$) via real-time RT-PCR (qRT-PCR). Furthermore, we knockdowned the MHRT gene by siRNA to confirm its roles in the $H_2O_2$-induced cardiac cell apoptosis, and found that knockdown of MHRT led to significant more apoptotic cells than the non-target control ($^{**}p<0.01$), indicating that the lncRNA MHRT is a protective factor for cardiomyocyte and the plasma concentration of MHRT may serve as a biomarker for myocardial infarction diagnosis in humans AMI.

• Korean Journal of Veterinary Research
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• v.39 no.1
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• pp.169-176
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• 1999

The purposes of this study were to set up the method of the natural killer(NK) cell activity assay using the flow cytometer and to examine the characteristics and distribution of the NK cell during rat hepatocarcinogenesis. Forty five male 6 week-old specific pathogen free(SPF) Sprague-Dawley rats were randomly divided into three groups. Group I was the non-treated control and given normal diet and water. Group II was treated with diethylnitrosamine(DEN, 200mg/kg, i.p.) and partial hepatectomy. Group III was treated with DEN, partial hepatectomy and 0.05% phenobarbital sodium in water from 3 to 16 weeks. All animals were examined the morphology of the large granular lymphocyte(LGL), the LGL percent of the total lymphocytes and the LGL conjugation rate with YAC-1 cell in peripheral blood, spleen and liver. Moreover, activity of the LGL isolated from peripheral blood lymphocytes was determined using the flow cytometer. As results, LGL were observed in the peripheral blood, spleen and liver. LGL were observed the relatively faintly staining basophilic cytoplasm with granules, and eccentric, often kidney-shaped nuclei in Giemsa stain. Its size was $11{\sim}13{\mu}m$. LGL percentage of the isolated lymphocytes in peripheral blood, spleen and liver were 1.8~2.3%, 1.3~1.4% and 0.87~0.99%, respectively. LGL conjugation rate with YAC-1 cell was shown to be peripheral blood(9.3~10.3 %) > spleen(7.7~8.7%) > liver(5.6~7.0%). The activity of the LGL isolated from peripheral blood lymphocytes in Group I, II and III was 33.7%, 30.5% and 35.4%, respectively. However, all values were not significantly between groups.

• Journal of Korean Society on Water Environment
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• v.31 no.3
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• pp.295-303
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• 2015

In Korea, Total Maximum Daily Loads(TMDLs) has been enforced to restore and manage water quality in the watersheds. However, some assesment of implementation plan of TMDLs showed that the achievement of the target water quality is not related to the proper allocation loads because difference of flow duration interval. In the United States, the discharge loads are determined by water quality modeling considering standard flow conditions according to purpose. Therefore, this study tried to develop the allocation method considering economical efficiency using water quality model. For this purpose, several allocation methods being used in the management of TMDLs is investigated and develope an allocation criteria considering regional equality and uniformity. Since WARMF(Watershed Analysis Risk Management Framework) model can simulate the time varying behavior of a system and the various water quality variables, it was selected for a decision support system in this study. This model showed fairly good performance by adequately simulating observed discharge and water quality in Miho watershed. Furthermore, the scenario simulation results showed that the effect of annual average water quality improvement to remove 1kg BOD is more than 25 times, even if point pollutants treatment facility is six times more expensive to operate than non-point pollutants treatment facility.

• Journal of Korean Society on Water Environment
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• v.33 no.6
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• pp.650-660
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• 2017

In this study, pollutant emission characteristics by water damage period analyzed 11 items (water temperature, pH, DO, EC, BOD, COD, TOC, SS, T-N, T-P and flow) with load duration curve, time series load curve and factor analysis for three years (2014-2016). Load duration curve is applied to judge the level of impaired waterbody and estimate impaired level by pollutants such as BOD and T-P in this study depending on variation of stream flow. Water quality standard exceeded the flow of mid-range and low-range by flow condition evaluation using load duration curve. This watershed was influenced by point source more than non-point source. Cumulative excess rate of BOD and T-P kept water quality standard for all seasons (spring, summer, autumn and winter) except BOD 59% in spring. Water quality changes were influenced by pollutants of basic environmental treatment facilities and agricultural areas during spring and summer. Results of factor analysis were classified commonly first factor (BOD, COD, and TOC) and second factor (flow, water temperature and SS). Therefore, effects of artificial pollutants and maintenance water must be controlled seasonally and reduced relative to water damage caused by point pollution sources with effluent standard strengthened in the target watershed.

• Proceedings of the Korean Vacuum Society Conference
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• 2012.02a
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• pp.346-346
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• 2012

We investigated electrical and optical properties and chemical states of amorphous In-Ga-Zn-O (a-IGZO) thin films deposited at different substrate temperatures (from room temperature to $300^{\circ}C$). a-IGZO thin films were fabricated by radio frequency magnetron sputtering using $In_2O_3$ : $Ga_2O_3$ : ZnO = 1 : 1 : 1 target, and their electrical and optical properties and chemical states were investigated by Hall-measurement, UV-visible spectroscopy and x-ray photoelectron spectroscopy (XPS), respectively. The data showed that as substrate temperature increased, carrier concentration increased, but mobility, conductivity, transmittance in the shorter wavelength region (>350 nm), and the Tauc-plot-estimated optical bandgap decreased. XPS data indicated that the intensity of In 3d peak compared to Ga 3d peak increased but the intensity of Zn 3d peak compared to Ga 3d decreased, and that, from the deconvoluted O 1s peak, defects or oxygen vacancies and non-quaternary contents increased as the temperature increased. The relative intensity changes of the In, Zn, and O 1s sub-component are suggested to explain the changes in electrical and optical properties.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.39 no.9
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• pp.839-847
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• 2011

This paper proposes a new guidance law for increasing the lethality of munitions. The well known PNG (Proportional Navigation Guidance) is inadequate for the munitions because of some weaknesses. Even if the munition does not have the impact point error, the acceleration command is non zero because the line-of-sight changes at all times in flight. Therefore, we use a difference between a target and an impact point. This proposed guidance law is similar to PNG in the form, but this guidance law concentrates a correction rate of flight path angle instead of the LOS (Line of Sight) rate. The correction of flight path angle is defined as the amount of impact point error. This impact point error can be calculated by neural networks rapidly. Finally, we show that the simulation results prove the suitability of this law.

• Proceedings of the Korean Radioactive Waste Society Conference
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• 2005.06a
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• pp.426-427
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• 2005

Advanced Spent Fuel Conditioning Process (ACP) is a pyrochemical process in which the spent fuel of PWR is transformed into the uranic metal ingot. Through this process, which has been developed in KAERI since 1998, the radioactivity, the radiotoxicity, the heat and the volume of the PWR spent fuel are reduced by a quarter of the original. To demonstrate a lab-scale process and extract the data for the later pilot-scale process, a demonstration facility of ACP (ACPF) is under construction and the lab-scale demonstration is slated for 2006. To establish the safeguardability of ACPF, a safeguards system including a neutron counter based on non-destructive assay, which is named as ACP Safeguards Neutron Counter (ASNC), the ACP Safeguards Surveillance System (ASSS) which consists of two neutron monitors and five IAEA cameras, and Laser Induced Breakdown System (LIBS) have been developed and are ready to be installed at ACPF. The target materials of ACP to assay with ASNC are categorized into three types among which the first is the uranic metal ingot, the second is the salt waste and the last is $UO_2$ and $U_{3}O_8$ powders, rod cuts and hulls. The Pu content of process nuclear materials can be accounted with ASNC. The ASSS is integrated in the ACP Intelligent Surveillance Software (AISS) in which the IAEA camera images and background signals at the rear doors of ACPF are displayed. The composition of special nuclear materials of ACP can be measured with LIBS which can be a supporting measurement tool for ASNC. The conceptual picture of safeguards system of ACPF is shown in Fig. 1.