• 대한의용생체공학회:의공학회지
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• 제43권2호
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• pp.71-80
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• 2022

The interaction between dual-ligand decorated particle-based delivery system and target cell under shear flow is predicted using probability model developed. We assumed the two kinds of ligand are decorated on the surface of the particle with 10% length difference. Fixed with other biophysical parameters, a study on the particle-cell interaction for the different non-specific interaction parameter is performed. To induce the firm adhesion, short ligand-receptor should be engaged. Also, it is shown that the rational design of ligand-receptor interaction, including receptor number, specific interaction parameter, kinds of ligand-receptor, etc., should be considered.

• The Korean Journal of Physiology and Pharmacology
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• 제12권5호
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• pp.281-286
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• 2008

Although the interaction between gp130 and the ErbB family has frequently been shown in cancer cells, the mechanism of this interaction remains unclear and controversial. In the present study, we found that specific tyrphostin inhibitors of ErbB2 (AG825 and AG879), but not ErbB1 inhibitor (AG1478), suppressed IL-6-induced tyrosine phosphorylation of STAT3 in schwannoma cells. However, biochemical evidence for transactivation of ErbB2 by IL-6 was not observed. Additionally, the inhibition of ErbB2 expression, with either a specific RNAi or transfection of an ErbB2 mutant lacking the intracellular domain did not inhibit the IL-6-induced tyrosine phosphorylation of STAT3. Thus, it seems that tyrphostins, which are known as specific inhibitors of the ErbB2 kinase, may have non-specific suppressive effects on the IL-6/STAT3 pathway.

• Journal of Ginseng Research
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• 제10권2호
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• pp.209-217
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• 1986

Yeast alcohol dehydrogenates and ginseng saponin interaction has been investigated to understand the non-specific enzyme stimulating effect of the saponin of Panax ginseng C.A. Meyer. It was confirmed that several amphiphiles such as sodium dodecyl sulfate(SDS), Triton X-100, sodium taurodeoxycholate (Na-TDC) as well as ginseng saponin mixture and purified ginseng glycosides lowered Km values of yeast alcohol dehydrogenase (ADH) for ethanol and NAD in the presence of the above amphiphiles suggesting that the surface activity of the amphiphiles might play a significant role in the ADH catalyzed reactions. Conformational change of yeast alcohol dehydrogenase in the presence of the above amphiphiles at their optimal concentration for the maximum activity was studied. Circular dichroism (C.D) spectrum of yeast ADH showed that the conformational change of the enzyme occurred in the presence of above amphiphiles. Fluorescence data also showed that the hydrophobic area increased in the presence of above amphiphiles. Examination of the interaction between ADH and ginseng saponin using radioactive saponin showed that there might be a very weak interaction between them. From the above results, it was concluded that the non-specific enzyme stimulating effect of the saponin might be due to the change of polarity of the enzyme solution in the presence of the saponin.

• International Journal of Computer Science & Network Security
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• 제22권4호
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• pp.47-52
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• 2022

The current study investigates how student-teacher interaction can be developed through task-based teaching in undergraduate students' Saudi teaching and learning context. An experiment was conducted for five weeks on 85 male undergraduate students at a Saudi public university based in Jeddah, Saudi Arabia. The study investigated different types of student-teacher interaction through task-based teaching (speaking activities). The results revealed that the experimental group (43 students) evinced much more enthusiasm, willingness, engagement and readiness in their inclass participation than their peers in the control group (42 students). The student-teacher interaction also helped students to be more responsive to general and specific topics in speaking activities. The study recommends that decision-makers in education make student-teacher interaction part of the student's monthly assessment. It also recommends that more efforts be made to foster the awareness of students, teachers, and parents awareness of the academic and non-academic importance of interaction. One final recommendation of the research is that student-teacher interaction should be more emphasized and integrated into the school curriculum and adopted as a critical teaching strategy.

• BMB Reports
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• 제37권2호
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• pp.153-158
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• 2004

The acid-labile subunit (ALS) is known to interact with the IGF binding protein (IGFBP) in the presence of insulin-like growth factors (IGFs). Studies, however, indicate that ALS forms a doublet with IGFBP3, independent of IGFs. To characterize the structural domain required for the IGF-free ALS-IGFBP3 interaction, seven recombinant human IGFBP3 mutants were generated: three deletion mutants and four site-specific mutants that had altering N-terminal regions of IGFBP3. ALS and IGFBP3 mRNAs were co-injected into Xenopus oocytes, and their products were cross-linked and immunoprecipitated using antisera against ALS or IGFBP3. Among the deletion mutants, the mutant of D40 (deleted in 11-40th amino acids) exerted no effect in the interaction with ALS, while D60 (${\Delta}11$-60) demonstrated a moderate reduction. D88 (${\Delta}11$-88), however, showed a significant decrease. In the case of site-specific mutants, the mutation that alterated the IGF binding site (codons 56 or 80) exerted a significant reduction in the interaction, whereas codons 72 or 87 showed no significant change in the interaction with ALS. The stability of the ALS-IGFBP3 interaction was analyzed according to a time-dependent mode. Consistent with the binding study, mutants on the IGF binding sites (56 or 80) consistently show a weakness in the ALS-IGFBP3 interaction when compared to the mutants that covered the non-IGF binding sites (72 or 87). This study suggests that the N-terminal of IGFBP3, especially the IGF binding site, plays an important role in interacting with ALS as well as in stabilizing the dual complex, independent of IGFs.

• 아동학회지
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• 제25권4호
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• pp.71-92
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• 2004

The purpose of this ethnographic study was to observe and understand interactions of toddlers and teachers in context. Specific questions were "What are the motives and features of the interactions between toddlers and teacher?" Subjects were twenty 2-year-old daycare children and their teachers. Methods covered 11 weeks of interviews and participatory observation. The major findings were that the interactions between toddlers and teachers was mainly initiated by toddlers and consist of physiological and emotional needs, conflict mediation and participation in play. As toddler get to the age of self-recognition but still need adult's assistance, the co-existence of dependence and independence was evident. Children used a variety of verbal and non-verbal signals with both positive and non-positive overtons.

• 한국철도학회논문집
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• 제18권4호
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• pp.363-373
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• 2015

본 논문은 휠-레일 사이의 접촉메카니즘을 이용하여 휠과 레일 사이의 분리를 허용하고, 횡 방향으로의 플랜지 접촉을 허용하는 비선형 동적 상호작용 해석법을 제시하는데 그 목적이 있다. 먼저 휠-레일 사이의 상호작용력을 압축스프링으로 이상화한 스프링 모델과 휠-레일 사이가 항상 접촉하고 있다는 가정하에 적용되는 non-jump모델에 대해 간략히 소개한 후 접촉메카니즘을 이용한 비선형 접촉모델에 대해 기술한다. 위 3가지 모델에 대한 수치해석 결과를 비교하기 위하여, 단순보 위를 지나가는 KTX열차에 대해 휠-레일 사이의 상호작용력을 각각 적용하여 동적 응답을 비교 분석하였다. 또한 특정 조건하에 플랜지 접촉을 강제 발생시켜 횡방향 응답의 변화를 살펴보았다.

• The Korean Journal of Physiology and Pharmacology
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• 제12권4호
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• pp.199-204
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• 2008

KIF1B${\beta}$ is a member of the Kinesin superfamily proteins (KIFs), which are microtubule-dependent molecular motors that are involved in various intracellular organellar transport processes. KIF1B${\beta}$ is not restricted to neuronal systems, however, is widely expressed in other tissues, even though the function of KIF1B${\beta}$ is still unclear. To elucidate the KIF1B${\beta}$-binding proteins in non-neuronal cells, we used the yeast two-hybrid system, and found a specific interaction of KIF1B${\beta}$ and the sorting nexin (SNX) 17. The C-terminal region of SNX17 is required for the binding with KIF1B${\beta}$. SNX17 protein bound to the specific region of KIF1Bf3 (813-916. aa), but not to other kinesin family members. In addition, this specific interaction was also observed in the Glutathione S-transferase pull-down assay. An antibody to SNX17 specifically co-immunoprecipitated KIF1B${\beta}$ associated with SNX17 from mouse brain extracts. These results suggest that SNX17 might be involved in the KIF1B${\beta}$-mediated transport as a KIF1B${\beta}$ adaptor protein.

• Journal of the Korean Physical Society
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• 제80권
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• pp.30-36
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• 2022

Holographic model of massive scalar field with its self-interaction λϕⁿ in AdS space is able to give a logarithmic scale dependence to marginal multi-trace deformation couplings on its dual conformal field theory, where λ is the self-interaction coupling of the scalar field, ϕ, and n is an integral number. In arXiv:1501.06664, the authors realize this feature by looking at bulk scalar solutions near AdS boundary imposing a specific boundary condition between the coefficients of non-normalizable and normalizable modes of the scalar field excitations. We study the same holographic model to see scale dependence of marginal deformations on the dual conformal field theory by employing completely different method: holographic Wilsonian renormalization group. We solve Hamilton-Jacobi equation derived from the holographic model of massive scalar with λϕⁿ interaction and obtain the solution of marginal multi-trace deformations up to the leading order in λ. It turns out that the solution of marginal multi-trace deformation also presents logarithmic behavior in energy scale near UV region.

• International Journal of Oral Biology
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• 제47권3호
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• pp.33-40
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• 2022

Store-operated Ca²⁺ entry (SOCE) represents one of the major Ca²⁺ entry routes in non-excitable cells. It is involved in a variety of fundamental biological processes and the maintenance of Ca²⁺ homeostasis. The Ca²⁺ release-activated Ca²⁺ (CRAC) channel consists of stromal interaction molecule and Orai; however, the role and action of Homer proteins as an adaptor protein to SOCE-mediated Ca²⁺ signaling through the activation of CRAC channels in non-excitable cells still remain unknown. In the present study, we investigated the role of Homer2 in the process of Ca²⁺ signaling induced by the interaction between CRACs and Homer2 proteins in non-excitable cells. The response to Ca²⁺ entry by thapsigargin-mediated Ca²⁺ store depletion remarkably decreased in pancreatic acinar cells of Homer2^-/- mice, as compared to wild-type cells. It also showed critical differences in regulated patterns by the specific blockers of SOCE in pancreatic acinar cells of Homer2^-/- mice. The response to Ca²⁺ entry by the depletion in Ca²⁺ store markedly increased in the cellular overexpression of Orai1 and STIM1 as compared to the overexpression of Homer2 in cells; however, this response was remarkably inhibited by the overexpression of Orai1, STIM1, and Homer2. These results suggest that Homer2 has a critical role in the regulatory action of SOCE activity and the interactions between CRAC channels.