• Journal of Microbiology and Biotechnology
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• 제25권1호
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• pp.127-136
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• 2015

Cupredoxin-like proteins are mainly copper-binding proteins that conserve a typical rigid Greek-key arrangement consisting of an eight-stranded β-sandwich, even though they share as little as 10-15% sequence similarity. The electron transport function of the Cupredoxins is critical for respiration and photosynthesis, and the proteins have therapeutic potential. Despite their crucial biological functions, the identification of the distant Cupredoxin homologs has been a difficult task due to their low sequence identity. In this study, the overlapped conserved residue (OCR) fingerprint for the Cupredoxin superfamily, which consists of conserved residues in three aspects (i.e., the sequence, structure, and intramolecular interaction), was used to detect the novel Cupredoxin homologs in the NCBI non-redundant protein sequence database. The OCR fingerprint could identify 54 potential Cupredoxin sequences, which were validated by scanning them against the conserved Cupredoxin motif near the Cu-binding site. This study also attempted to model the 3D structures and to predict the functions of the identified potential Cupredoxins. This study suggests that the OCR-based approach can be used efficiently to detect novel homologous proteins with low sequence identity, such as Cupredoxins.

• Structural Engineering and Mechanics
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• 제44권3호
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• pp.289-303
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• 2012

Existing plane stress solutions for thin plates and disks have shown several qualitative features which are difficult to handle with the use of commercial numerical codes (non-existence of solutions, singular solutions, rapid growth of the plastic zone with a loading parameter). In order to understand the effect of temperature and pressure-dependency of the yield criterion on some of such features as well as on the distribution of residual stresses and strains, a semi-analytic solution for a thin hollow disk fixed to a rigid container and subject to thermal loading and subsequent unloading is derived. The material model is elastic-perfectly/plastic. The Drucker-Prager pressure-dependent yield criterion and the equation of incompressibity for plastic strains are adopted. The distribution of residual stresses and strains is illustrated for a wide range of the parameter which controls pressure-dependency of the yield criterion.

• Steel and Composite Structures
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• 제50권1호
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• pp.77-87
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• 2024

In this study, the two-dimensional crack problem was investigated by using the finite element method (FEM)-based ANSYS package program and the artificial neural network (ANN)-based multilayer perceptron (MLP) method. For this purpose, a half-infinite functionally graded (FG) layer with a crack pressed through two rigid blocks was analyzed using FEM and ANN. Mass forces and friction were neglected in the solution. To control the validity of the crack problem model exercised, the acquired results were compared with a study in the literature. In addition, FEM and ANN results were checked using Root Mean Square Error (RMSE) and coefficient of determination (R²), and a well agreement was found. Numerical solutions were made considering different geometric parameters and material properties. The stress intensity factor (SIF) was examined for these values, and the results were presented. Consequently, it is concluded that the considered non-dimensional quantities have a noteworthy influence on the SIF. Also FEM and ANN can be logical alternative methods to time-consuming analytical solutions if used correctly.

• Advances in nano research
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• 제15권4호
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• pp.339-353
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• 2023

This work aims to present a solution for the buckling behavior of perforated nano/microbeams with deformable boundary conditions using nonlocal strain gradient theory (NLSGT). For the first time, a solution that can provide buckling loads based on the non-local and strain gradient effects of perforated nanostructures on an elastic foundation, while taking into account both deformable and rigid boundary conditions. Stokes' transformation and Fourier series are used to realize this aim and determine the buckling loads under various boundary conditions. We employ the NLSGT to account for size-dependent effects and utilize the Winkler model to formulate the elastic foundation. The buckling behavior of the perforated nano/microbeams restrained with lateral springs at both ends is studied for various parameters such as the number of holes, the length and filling ratio of the perforated beam, the internal length, the nonlocal parameter and the dimensionless foundation parameter. Our results indicate that the number of holes and filling ratio significantly affect the buckling response of perforated nano/microbeams. Increasing the filling ratio increases buckling loads, while increasing the number of holes decreases buckling loads. The effects of the non-local and internal length parameters on the buckling behavior of the perforated nano/microbeams are also discussed. These material length parameters have opposite effects on the variation of buckling loads. This study presents an effective eigenvalue solution based on Stokes' transformation and Fourier series of the restrained nano/microbeams under the effects of elastic medium, perforation parameters, deformable boundaries and nonlocal strain gradient elasticity for the first time.

• Wind and Structures
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• 제31권2호
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• pp.143-152
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• 2020

The wind design of buildings is typically based on strength provisions under ultimate loads. This is unlike the ductility-based approach used in seismic design, which allows inelastic actions to take place in the structure under extreme seismic events. This research investigates the application of a similar concept in wind engineering. In seismic design, the elastic forces resulting from an extreme event of high return period are reduced by a load reduction factor chosen by the designer and accordingly a certain ductility capacity needs to be achieved by the structure. Two reasons have triggered the investigation of this ductility-based concept under wind loads. Firstly, there is a trend in the design codes to increase the return period used in wind design approaching the large return period used in seismic design. Secondly, the structure always possesses a certain level of ductility that the wind design does not benefit from. Many technical issues arise when applying a ductility-based approach under wind loads. The use of reduced design loads will lead to the design of a more flexible structure with larger natural periods. While this might be beneficial for seismic response, it is not necessarily the case for the wind response, where increasing the flexibility is expected to increase the fluctuating response. This particular issue is examined by considering a case study of a sixty-five-story high-rise building previously tested at the Boundary Layer Wind Tunnel Laboratory at the University of Western Ontario using a pressure model. A three-dimensional finite element model is developed for the building. The wind pressures from the tested rigid model are applied to the finite element model and a time history dynamic analysis is conducted. The time history variation of the straining actions on various structure elements of the building are evaluated and decomposed into mean, background and fluctuating components. A reduction factor is applied to the fluctuating components and a modified time history response of the straining actions is calculated. The building components are redesigned under this set of reduced straining actions and its fundamental period is then evaluated. A new set of loads is calculated based on the modified period and is compared to the set of loads associated with the original structure. This is followed by non-linear static pushover analysis conducted individually on each shear wall module after redesigning these walls. The ductility demand of shear walls with reduced cross sections is assessed to justify the application of the load reduction factor "R".

• 콘크리트학회논문집
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• 제22권3호
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• pp.381-388
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• 2010

이 논문에서는 내진상세가 적용되지 않은 RC골조의 지진 거동 특성을 파악하였다. 해석 대상 건물은 내진 규준의 적용을 받지 않고 중력하중만을 고려하여 설계되었다. 원형철근이 주철근으로 사용되었으며, 부재는 낮은 수준의 전단력을 견딜 수 있는 최소한의 스터럽이 사용되어 코어 부분의 구속효과는 거의 없다. 평면비정형성을 가진 건물의 경우, 푸쉬오버 해석을 통해서는 비틀림으로 인한 평면상에서 연단부의 손상집중을 파악할 수 없으므로 비선형 동적해석을 사용하는 것이 바람직하다. 섬유요소를 이용한 비선형 동적해석은 양방향 지진하중과 비틀림 거동의 영향을 받는 RC골조의 거동을 성공적으로 예측할 수 있었다. 하지만, 보다 진보된 응답 예측을 위해서는 부착 미끄러짐과 같은 보-기둥 접합부의 국부거동을 정밀하게 나타내는 모델링 요소의 개발이 필요하다.

• Ocean and Polar Research
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• 제36권4호
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• pp.495-503
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• 2014

This paper focuses on the dynamic responses of a tracked vehicle crawling on extremely cohesive soft soil, each side of which is composed of two parallel tracks. The tracked vehicle consisted of 2 bodies. One body is the tracked vehicle body, which is assumed to be a rigid body with 6 DOFs. The other body is the buoy body. The two bodies are connected by a revolute joint. In order to evaluate the travelling performance of a 7 DOFs vehicle, a dynamic analysis program for the tracked vehicle was developed using Newmark's method and the incremental-iterative method. The effects of road wheels on the track and soil are not taken into account. A terra-mechanics model of extremely cohesive soft soil is implemented in form of relationships: normal pressure to sinkage, shear resistance to shear displacement, and dynamic sinkage to shear displacement. Pressure-sinkage relationship and shear displacement-stress relationship should represent the non-linear characteristics of extremely soft soil. Especially, since the shear resistance of soft soil is very sensitive to shear displacement, spatial distribution of shear displacement occurring at the contact area of the tracks should be calculated precisely. The proposed program is developed in FORTRAN.

• Journal of information and communication convergence engineering
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• 제11권3호
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• pp.207-215
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• 2013

In this paper, we address the challenging computer vision problem of obtaining a reliable facial expression analysis from a naturally interacting person. We propose a system that combines a 3D generic face model, 3D head tracking, and 2D tracker to track facial landmarks and recognize expressions. First, we extract facial landmarks from a neutral frontal face, and then we deform a 3D generic face to fit the input face. Next, we use our real-time 3D head tracking module to track a person's head in 3D and predict facial landmark positions in 2D using the projection from the updated 3D face model. Finally, we use tracked 2D landmarks to update the 3D landmarks. This integrated tracking loop enables efficient tracking of the non-rigid parts of a face in the presence of large 3D head motion. We conducted experiments for facial expression recognition using both framebased and sequence-based approaches. Our method provides a 75.9% recognition rate in 8 subjects with 7 key expressions. Our approach provides a considerable step forward toward new applications including human-computer interactions, behavioral science, robotics, and game applications.

• Journal of Advanced Marine Engineering and Technology
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• 제37권6호
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• pp.617-622
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• 2013

해양 부유체 내부 유체는 파랑에 의한 외력을 받게 되면 슬로싱(sloshing)이 발생하게 된다. 부유체의 슬로싱에 의한 영향을 해석하기 위해서, 파랑에 의한 부유체의 거동뿐만 아니라 슬로싱에 의한 부유체의 응답을 고려한 결합적인 해석이 필요하다. 전산유체역학(CFD) 해석에 있어서, 외란은 비선형 파랑인 Stokes 5차 이론을 사용했고, 유동 해석은 Navier-Stokes 방정식과 Shear-Stress Transport(SST) 난류 모델을 이용하였다. 해양 부유체는 Pitch, Heave 운동에 대한 2자유도 해석을 진행 하였고, 결과에서는 슬로싱을 포함한 강체 운동을 확인 할 수 있다.

• 한국지진공학회논문집
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• 제13권2호
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• pp.59-68
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• 2009

안전하고 경제적인 내진설계를 위해서는 설계 초기단계부터 모멘트재분배와 부재소성변형을 고려하는 것이 바람직하다. 본 연구에서는 할선강성해석을 사용하여 각 부재의 재분배된 모멘트와 소성변형을 직접적으로 고려할 수 있는 내진설계방법을 개발하였다. 모멘트재분배에 의하여 발생된 비대칭 부재강성을 나타내기 위하여, 비강접 단부접합부를 갖는 보-기둥요소(NREC요소)를 사용하여 구조물을 모델링하였다. NREC요소에 사용되는 할선강성은 건물 및 부재의 요구연성도에 기반하여 결정하였다. 할선강성 구조모델에 대한 선형해석을 수행하여 내진설계를 위한 부재력과 소성변형을 구하였다. 본 연구에서는 할선강성해석을 모멘트골조와 이중골조의 내진설계에 적용하였고, 설계결과를 정밀한 비선형해석 결과와 비교하였다.