• Title/Summary/Keyword: Non-labeling

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Genome Detection Using an Integrated type DNA Chip Microelectrode-array and Non-labeling Target DNA (집적형 DNA칩 미소 전극 어레이 및 비수식화 표적 DNA를 이용한 유전자 검출)

  • Choi, Yong-Sung;Lee, Hea-Yeon;Tanaka, Hiroyuki;Tanaka, Hidekafu;Kwon, Young-Soo;Kawai, Tomoii
    • Proceedings of the KIEE Conference
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    • 2001.11a
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    • pp.274-276
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    • 2001
  • This research aims to develop the multiple channel electrochemical DNA chip using microfabrication technology. At first, we fabricated a high integration type DNA chip array by lithography technology. Several probe DNAs consisting of thiol group at their 5-end were immobilized on the sold electrodes. Then target DNAs were hybridized and reacted. Cyclic voltammetry showed a difference between target DNA and control DNA in the anodic peak current values. Therefore, it is able to detect a plural genes electrochemically after immobilization of a plural probe DNA and hybridization of non-labeling target DNA on the electrodes simultaneously. It suggested that this DNA chip could recognize the sequence specific genes.

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Reconstruction and Elimination of Optical Microscopic Background Using Surface Fitting Method

  • Kim Hak-Kyeong;Kim Dong-Kyu;Jeong Nam-Soo;Lee Myung-Suk;Kim Sang-Bong
    • Fisheries and Aquatic Sciences
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    • v.4 no.1
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    • pp.10-17
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    • 2001
  • One serious problem among the troubles to identify objects in an optical microscopic image is contour background due to non-uniform light source and various transparency of samples. To solve this problem, this paper proposed an elimination method of the contour background and compensation technique as follows. First, Otsu's optimal thresholding method extracts pixels representing background. Second, bilinear interpolation finds non-deterministic background pixels among the sampled pixels. Third, the 2D cubic fitting method composes surface function from pivoted background pixels. Fourth, reconstruction procedure makes a contour image from the surface function. Finally, elimination procedure subtracts the approximated background from the original image. To prove the effectiveness of the proposed algorithm, this algorithm is applied to the yeast Zygosaccharomyces rouxii and ammonia-oxidizing bacteria Acinetobacter sp. Labeling by this proposed method can remove some noise and is more exact than labeling by only Otsu's method. Futhermore, we show that it is more effective for the reduction of noise.

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Status and Response Strategies of Carbon Labeling in Landscape Architecture (조경분야 탄소성적표지제도 적용실태 및 대응전략)

  • Kim, Jeong-Ho;Yoon, Yong-Han
    • Journal of Environmental Science International
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    • v.24 no.6
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    • pp.709-720
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    • 2015
  • After analyzing the actual acquisition status of carbon labeling by year and by product for the past four years, as well as its certification in the construction-related sectors of greenhouse gas emission, this study attempted to present the problems and coping strategies upon issuing the carbon labeling certification in the landscape architecture. During the period of this analysis, the carbon labelings were acquired by 134 enterprises, 267 workplaces, and 735 products, while the percentage of acquisition was highest in the regular non-durable goods(49%), followed by energy-consuming durable goods(26%), regular production goods (19%), regular durable goods(3%), and service(3%). Furthermore, the acquisition certifications in construction sectors, were highest in the various pipes/panel(8 cases), followed by concrete(6 cases), gypsum board(4 cases), and landscape architecture materials(2 cases). The landscape architecture only had two cases in the acquisition certification for the first time in 2012, which accounted for 0.27% of the entire certification products, due to the uncertainty in the process, the lack of professionalism, and the lack of comprehension. However, the study conducted on the coping strategies for carbon labeling in the landscape architecture revealed the following: (1) regular reporting system management through the division of labor in the head office and factories, (2) the building of objective DB through the adoption of data management programs such as SAP, (3) continuous promotion and vitalization of the incentive system, (4) the adoption of mandatory or preferential application system in landscaping projects, management, and bidding, (5) enhancement of elasticity in deliberation of certification by recruiting experts in the landscape architecture sectors, and (6) provision of incentives for the cooperative firms acquiring the certification and support for their participation.

Perceptual development in the categorization of pitch accent contrasts in children and adults

  • Kim, Jung-Sun
    • Phonetics and Speech Sciences
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    • v.3 no.3
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    • pp.11-18
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    • 2011
  • This paper examines the categorical labeling of lexical pitch accent contrasts in North Kyungsang and South Cholla Korean listeners. It focuses specifically on investigating whether the pitch accent perception of adults and children has a dialect-specific effect. To evaluate the development of perceptual identification, slopes, intercepts, and positions at categorical boundaries were computed using a logistic regression function. The results showed that differences in slopes and intercepts were significant between North Kyungsang child and adult listeners, but the same was not the case for the positions at boundaries. As far as South Cholla child and adult listeners were concerned, there was a significant difference in slopes, but not intercepts and positions at boundaries. In the present study, the comparison of intercepts and slopes at the boundaries indicated developmental differences between North Kyungsang adult and child listeners. This improvement in categorical proportion seems to be a result of developmental changes in categorical perception. For South Cholla adult and child listeners, however, perception of the non-native contrast becomes less categorical.

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Conversion of 2D to 3D image using Object extraction and Non-local filter (객체 추출과 Non-Local 필터를 이용한 2D 영상의 3D 변환)

  • Kang, Keun-Ho;Lee, Wang-Ro;Yoo, Jisang
    • Proceedings of the Korean Society of Broadcast Engineers Conference
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    • 2010.11a
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    • pp.184-187
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    • 2010
  • 본 논문에서는 움직임 추정(Motion Estimation, ME), 색상 라벨링(Labeling) 그리고 Non-Local means 필터 등을 이용하여 2D 영상을 3D 입체 영상으로 변환하는 기법을 제안한다. 제안하는 기법에서는 프레임 간의 움직임 추정 방법을 사용하여 물체의 움직임 벡터를 추출하며 색상 라벨링 작업을 통해 세밀한 객체를 추출한다. 객체를 추출한 후 영상을 이동시켜서 우영상을 생성한다. 우 영상을 생성하는 과정에서 채워지지 않은 화소들이 발생하는데 전체 화소의 상관도를 고려하는 Non-local means 필터를 사용하여 이 부분을 처리한다. 생성된 우 영상과 원본 영상인 좌 영상으로 비월주사(interlace)하여 최종 3D 입체 영상을 생성한다.

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Central energy metabolism remains robust in acute steatotic hepatocytes challenged by a high free fatty acid load

  • Niklas, Jens;Bonin, Anne;Mangin, Stefanie;Bucher, Joachim;Kopacz, Stephanie;Matz-Soja, Madlen;Thiel, Carlo;Gebhardt, Rolf;Hofmann, Ute;Mauch, Klaus
    • BMB Reports
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    • v.45 no.7
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    • pp.396-401
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    • 2012
  • Overnutrition is one of the major causes of non-alcoholic fatty liver disease (NAFLD). NAFLD is characterized by an accumulation of lipids (triglycerides) in hepatocytes and is often accompanied by high plasma levels of free fatty acids (FFA). In this study, we compared the energy metabolism in acute steatotic and non-steatotic primary mouse hepatocytes. Acute steatosis was induced by pre-incubation with high concentrations of oleate and palmitate. Labeling experiments were conducted using [$U-^{13}C_5$,$U-^{15}N_2$] glutamine. Metabolite concentrations and mass isotopomer distributions of intracellular metabolites were measured and applied for metabolic flux estimation using transient $^{13}C$ metabolic flux analysis. FFAs were efficiently taken up and almost completely incorporated into triglycerides (TAGs). In spite of high FFA uptake rates and the high synthesis rate of TAGs, central energy metabolism was not significantly changed in acute steatotic cells. Fatty acid ${\beta}$-oxidation does not significantly contribute to the detoxification of FFAs under the applied conditions.

Warning Labels on Cigarette Packages: A Special Stimulus for Moslem Smokers to Quit Smoking

  • Halim, Rizal Edy;Sumiyarto, Sumiyarto;Muttaqin, Faisal
    • Asian Journal of Business Environment
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    • v.5 no.1
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    • pp.5-11
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    • 2015
  • Purpose - This study aims to explore the influence of combining "non-halal" labels with visual and textual warning labels on cigarette packages to induce the intention to quit smoking and boost the stop-smoking campaign. Research design, data, and methodology - This study examines"non-halal" labeling on cigarette packages using an experimental method. A total of 120 smokers, aged 18-23, were chosen from among Universitas Indonesia students. Data obtained were analyzed using ANOVA and T-Test. Results - The use of a "non-halal" label as a warning on cigarette packages is more effective to influence Muslim smokers to quit smoking. The results also suggest that "non-halal" labels more effectively increase intentions to quit smoking when use din combination with textual-visual labels. Conclusions - The study found that the addition of "non-halal" labels in textual or textual-visual warning labels on cigarette packages would significantly increase the intention of Moslems smokers to quit smoking. These results support previous research findings, that if cigarettes are labeled as "non-halal" (haram) products for Moslem teenagers, it will induce them to quit smoking.

Input Device of Non-Touch Screen Using Vision (비전을 이용한 비접촉 스크린 입력장치)

  • Seo, Hyo-Dong;Joo, Young-Hoon
    • The Transactions of The Korean Institute of Electrical Engineers
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    • v.60 no.10
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    • pp.1946-1950
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    • 2011
  • This paper deals with an input device without the touch. The existing touch screens have some problems such as the week durability by frequent contact and the high cost by complex hardware configuration. In this paper, a non-touch input device is proposed to overcome these problems. The proposed method uses a skin color generated by the HCbCr color model and a hand region obtained by the labeling technique. In Addition, the skeleton model is employed to improve the recognition performance of the hand motion. Finally, the experiment results show the applicability of the proposed method.

Design of an Effective Deep Learning-Based Non-Profiling Side-Channel Analysis Model (효과적인 딥러닝 기반 비프로파일링 부채널 분석 모델 설계방안)

  • Han, JaeSeung;Sim, Bo-Yeon;Lim, Han-Seop;Kim, Ju-Hwan;Han, Dong-Guk
    • Journal of the Korea Institute of Information Security & Cryptology
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    • v.30 no.6
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    • pp.1291-1300
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    • 2020
  • Recently, a deep learning-based non-profiling side-channel analysis was proposed. The deep learning-based non-profiling analysis is a technique that trains a neural network model for all guessed keys and then finds the correct secret key through the difference in the training metrics. As the performance of non-profiling analysis varies greatly depending on the neural network training model design, a correct model design criterion is required. This paper describes the two types of loss functions and eight labeling methods used in the training model design. It predicts the analysis performance of each labeling method in terms of non-profiling analysis and power consumption model. Considering the characteristics of non-profiling analysis and the HW (Hamming Weight) power consumption model is assumed, we predict that the learning model applying the HW label without One-hot encoding and the Correlation Optimization (CO) loss will have the best analysis performance. And we performed actual analysis on three data sets that are Subbytes operation part of AES-128 1 round. We verified our prediction by non-profiling analyzing two data sets with a total 16 of MLP-based model, which we describe.

FISH기법 적용을 위한 Y 염색체 특이 DNA Probe의 개발

  • 조은정;류란숙;류은경;손시환
    • Proceedings of the KSAR Conference
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    • 2003.06a
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    • pp.24-24
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    • 2003
  • Fluorescence in situ Hybridization(FISH)는 특정 염기서열을 이용하여 염색체나 염색체상의 DNA위치를 확인하는 기술로서, 면역세포화학 기술과 결합되어져 현미경으로 이들의 유전적 활성도를 직접 확인할 수 있는 방법으로 지금까지의 radioisotopes 대신 non-radioactive labeling 방법으로서 fluorescence을 이용한 분자세포유전학적 검정 방법이다. 따라서 특정 염색체의 FISH probe의 개발은 FISH 기법을 이용하여 조직 또는 세포내 특정 염색체나 DNA의 존재나 이상 유무를 신속하고 정확하게 파악할 수 있다. 본 연구는 소와 사람을 대상으로 Y-염색체 특이 DNA probe를 개발하고 이를 이용하여 FISH를 시행함으로서 본 probe의 신뢰성을 확인하고 임상적 적용 가능성을 제시 하고자 하였다.

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