• 한국임상수의학회지
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• 제18권4호
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• pp.380-389
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• 2001

Non-human primates have been increasing in demand as important experimental animals and companion animals, domestically and internationally. The number of non-human primates for these purposes will be much enhanced in the near future. Despite this trend, basic physiological data are scarcely available in these animal species, leading to the difficulty to diagnose diseases when necessary, due to the absence of reference values. Particularly, there is not any report on the total activity of LDH of non-human primates, let alone LDH isoenzyme patterns, in Korea. LDH isoenzymes have a high level of efficaciousness as diagnostic and prognostic aids in various diseases. In this study, total activities and isoenzyme patterns of LDH were measured to obtain their reference values in domestically reared common marmosets, crab-eating macaques and Japanese macaques. There were widespread different values of serum total LDH among the non-human primate species experimented in this study. Serum LDH values of common marmosets and crab-eating macaques were 597.5$\pm$243.1 IU/l and 605.3$\pm$312.6 IU/l, respectively, whereas those of Japanese macaque showed 1,209$\pm$473.8 IU/l. Five isoenzyme fractions of LDH were observed in all experimented non-human primates but their ranks and proportions represented different patterns one another. In common marmosets, the percent of fraction for serum LDH1, LDH$_2$, LDH$_3$, LDH$_4$, and LDH$_{5}$ was 13.7$\pm$6.4%, 23.3$\pm$3.6%, 29.2$\pm$5.0%, 9.4$\pm$1.4% and 24.4$\pm$7.5%, respectively. The rank of LDH isoenzymes was LDH$_3$>LDH$_{5}$>LDH$_2$>LDH$_1$>LDH$_4$, in the descending order. For crab-eating macaques, the fraction of serum LDH$_1$, LDH$_2$, LDH$_3$, LDH$_4$, and LDH$_{5}$ occupied 19.5$\pm$12.7%, 25.3$\pm$9.3%, 23.8$\pm$8.1%, 10.2$\pm$2.8% and 21.3$\pm$14.2%, respectively. The order of LDH isoenzymes was LDH$_2$>LDH$_3$>LDH$_{5}$>LDH$_1$>LDH$_4$, from top to down. On the while, in Japanese macaques, the fraction of serum LDH$_1$ to LDH$_{5}$ showed 23.4$\pm$11.8%, 30.5$\pm$4.1%, 17.4$\pm$3.9%, 11.3$\pm$3.7% and 13.8$\pm$5.6%, respectively. The decreasing order indicated LDH$_2$>LDH$_1$>LDH$_3$>LDH$_{5}$>LDH$_4$. In conclusion, values such as LDH and LDH isoenzyme patterns of investigated for the first time from non-human primates reaared in Korea, could be reference values for the optimal diagnosis and therapy of diseases of the corresponding animal species. Other parameters of hematology and blood biochemistry are urgently needed to study for the benefit of our intimate non-human primates.an primates.

• 한국임상수의학회지
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• 제18권4호
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• pp.390-396
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• 2001

CKP isoenzymes have a high level of efficaciousness as diagnostic and prognostic aids in various diseases. There is not any report on the total activity of CPK of non-human primates, let alone CPK isoenzyme patterns, in Korea. In this study, total activities and isoenzyme patterns of CPK were measured to obtain their reference values in domestically reared common marmosets, crab-eating macaques and Japanese macaques. We observed remarkable different values of serum total CPK from the primates used in this experiment. Serum CPK activities of Japanese macaques and crab-eating macaques were 275.8$\pm$158.1 IU/l and 396.7$\pm$697.4 IU/l, respectively, whereas those of common marmosets showed much higher value of 618.8$\pm$1,117.6 IU/l. In all common marmosets and crab-eating macaques, only CPK$_3$ ws observed. In five out of eight Japanese macaques, CPK$_3$ was the sole fraction but two animals showed CPK$_1$ and CPK$_3$ isoenzymes, and the remaining one had CPK$_2$ and CPK$_3$ fractions. There were some discrepancies in the pattern and ratio of isoenzyme fractions in Japanese macaques. In conclusion, values such as CPK and CPK isoenzyme patterns of investigated for the first time form non-human primates reared in Korea, could be reference values for the optimal diagnosis and therapy diseases of the corresponding animal species.

• 한국임상수의학회지
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• 제19권2호
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• pp.132-138
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• 2002

Non-human primates are widely used for experimental animal and raised as companion animal in Korea. To establish the electrocadiogram (ECG) of non-human primates that are domestically raised, the author measured bipolar limb leads and augmented unipolar limb leads, after tiletamine/zolazepam (TZ) injection as sedative agents. The amplitudes of P,Q, R,S and T wave and duration time of P wave, QRS complex, PR and QT interval in each lead of ECG were evaluated in 7 non-human primates at 15 minutes after TZ injection, respectively. The amplitude of P wave in I, II,III, aVR, aVL and aVF leads revealed 0.06$\pm$ 0.05,0.14$\pm$ 0.05, 0.1 $\pm$ 0.05,-0.11 $\pm$ 0.06,-0.04$\pm$ 0.04 and 0.12$\pm$ 0.05 mV respectively. The amplitude of Q wave revealed -0.16$\pm$ 0.15, -0.23$\pm$ 0.18, -0.17$\pm$ 0.13, 0.16$\pm$0.13, 0.04$\pm$ 0.09 and -0.2$\pm$0.13 mV, respectively. The amplitude of R wave revealed 0.56$\pm$0.56, 1.24$\pm$ 0.67, 0.92$\pm$0.33, -0.37$\pm$ 1.14, -0.22$\pm$ 0.47 and 1.12 $\pm$ 0.47 mV, respectively. The amplitude of S wave revealed -0.02$\pm$ 0.08, -0.04$\pm$0.06, -0.06$\pm$0.04, 0.02$\pm$0.04, 0.04$\pm$0.09 and -0.04 $\pm$ 0.06 mV, respectively. The amplitude of T wave revealed -0.01 $\pm$ 0.15,-0.02$\pm$ 0.13, 0.01 $\pm$ 0.08, 0.02$\pm$ 0.12, 0.01 $\pm$ 0.11 and -0.03$\pm$ 0.09 mV, respectively. The duration time of P wave revealed 0.05 $\pm$ 0.01, 0.04$\pm$ 0.01, 0.05$\pm$ 0.02, 0.05 $\pm$ 0.02, 0.04$\pm$ 0.01 and 0.04$\pm$ 0.01 sec, respectively. The duration time of QRS complex revealed 0.05 $\pm$ 0.02,0.05$\pm$ 0.01, 0.05 $\pm$ 0.01, 0.04$\pm$ 0.01, 0.05$\pm$ 0.01 and 0.05 $\pm$ 0.01 sec, respectively. The duration time of PR interval revealed 0.08$\pm$ 0.01, 0.07$\pm$0.01,0.08$\pm$ 0.03, 0.08$\pm$0.01, 0.08$\pm$ 0.01 and 0.08$\pm$0.01 sec, respectively. The duration time of QT interval revealed 0.23$\pm$ 0.06, 0.22$\pm$ 0.05, 0.23 $\pm$ 0.06, 0.23$\pm$ 0.06, 0.24$\pm$ 0.05 and 0.22$\pm$ 0.02 sec, respectively. No significant changes were observed in e amplitude of P and T waves. The amplitude of QRS complex in ketamine group was higher than that of TZ group. However, no significant changes were observed in both intra-group and inter-group. There were no significant changes in the duration time of P wave, QRS complex and PR interval obtained from both groups. Also, the duration time of QT interval in TZ group was significantly longer at 30 min.(P< 0.05) an that of 10 minutes after injection. However, significant difference was not found between two groups. The mean cardiac electric axis in ketamine group tended to decrease until 30 min. after injection and then gradually increase. However, mean cardiac electric axis of TZ group was increased until 30 min. after injection and then decreased. But significant differences were not observed between groups. These results suggest that the ECG pattern after TZ injection to non-human primates reared in korea was established. It was also considered that the injection of ketamine and TZ didn't significantly affect on ECG pattern of the non-human primates.

• 한국임상수의학회지
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• 제17권1호
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• pp.88-92
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• 2000

In Everland Zoological Gardens, the mortality by extrinsic cause in non-human primates during 1976∼1999 were retrospectively analyzed based on the clinical charts and/or autopsy reports. The number of deaths from extrinsic factor was 61 among a total of 161 monkeys which were died during that period. Among 61 monkeys of death from extrinsic factor, the number at a detailed cause were as follows: strangulation, 17(27.87%); accident fall, 15(24.59%); suffocation, 13(21.31%); drowning, 7(11.48%); death from pressure, 2(3.28%); collision, 2(3.28%); sunstroke, 1(64%); starvation, 1(1.64%); freezing to death, 1(1.64%); contusion, 1(1.64%). The number of deaths from extrinsic factor was 39 among a total of 81 squirrel monkeys which were died during that period. Among 39 squirrel monkeys of death from extrinsic factor, the number at a detailed cause were as follows; suffocation, 11(28.21%); accident fall, 8(20.51%); strangulation, 7(17.95%); drowning, 7(17.95%); death from pressure, 2(5.13%); starvation, 1(2.56%); collision, 1(2.56%). The number of deaths from extrinsic factor was 14 among a total of 50 Japanese macaque died during that period. Among 14 Japanese macaque from extrinsic factor, the number at a detailed cause were as follows; strangulation, 7(50.55%); accident fall, 6(42.85%); suffocation, 1(7.14%). It was considered that far facilities, adequate space and suitable indoor temperature are needed for the prevention of deaths of extrinsic cause at the monkey raising in zoological gardens or research center.

• IMMUNE NETWORK
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• 제10권6호
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• pp.198-205
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• 2010

Background: A crucial limitation of DNA vaccines is its weak immunogenicity, especially in terms of eliciting antibody responses in non-human primates or humans; therefore, it is essential to enhance immune responses to vaccination for the development of successful DNA vaccines for humans. Methods: Here, we approached this issue by evaluating interleukin-7 (IL-7) as a genetic adjuvant in cynomolgus monkeys immunized with multigenic HCV DNA vaccine. Results: Codelivery of human IL-7 (hIL-7)-encoding DNA appeared to increase DNA vaccine-induced antibody responses specific for HCV E2 protein, which plays a critical role in protecting from HCV infection. HCV-specific T cell responses were also significantly enhanced by codelivery of hIL-7 DNA. Interestingly, the augmentation of T cell responses by codelivery of hIL-7 DNA was shown to be due to the enhancement of both the breadth and magnitude of immune responses against dominant and subdominant epitopes. Conclusion: Taken together, these findings suggest that the hIL-7-expressing plasmid serves as a promising vaccine adjuvant capable of eliciting enhanced vaccine-induced antibody and broad T cell responses.

• 한국수정란이식학회:학술대회논문집
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• 한국수정란이식학회 2004년도 제4회 발생공학 국제심포지움 및 학술대회
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• pp.32-32
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• 2004

• 대한약학회:학술대회논문집
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• 대한약학회 2000년도 NEW STRATEGY FOR DRUG DEVELOPMENT IN POST-GENOMIC ERA(대한약학회)
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• pp.181.2-182
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• 2000

• 한국임상수의학회지
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• 제18권4호
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• pp.397-401
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• 2001

Blood gas values such as pO$_2$ were studied in common marmosets, crab-eating macaques and Japanese macaques, rhesus macaques and celebes macaque reared in Korea. Blood pH and blood gas values were evaluated in both arterial and venous blood. pH, p$CO_2$, and pO$_2$, of arterial blood in common marmosets were measured as 7.4$\pm$0.1, 29.2$\pm$3.6 mmHg and 81.5$\pm$8.9 mmHg, respectively. Corresponding values in one crab-eating macaque were 7.3, 41.3 mmHg and 46.5 mmHg, respectively. In case of venous blood, pH, p$CO_2$, and pO$_2$, in common marmosets were observed as 7.2$\pm$0.2, 64.9$\pm$18.3 mmHg and 23.5$\pm$5.4 mmHg, respectively. On the while, pH, p$CO_2$, and pO$_2$, of venous blood in crab-eating macaques showed 7.2$\pm$0.2, 49.9$\pm$8.0 mmHg and 38.3$\pm$8.8 mmHg, respectively. Venous pH, p$CO_2$, and pO$_2$, in Japanese macaques were 7.1$\pm$0.2, 56.4$\pm$5.3 mmHg and 40.1$\pm$9.3 mmHg, respectively. Those values in one rhesus macaque were 7.2, 61.1 mmHg and 24.9 mmHg, and in celebes macaque were 7.1, 54.3 mmHg and 31.8 mmHg, respectively.

• Parasites, Hosts and Diseases
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• 제53권4호
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• pp.395-402
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• 2015

Non-human primates (NHPs) are confirmed as reservoirs of Cryptosporidium spp., Giardia intestinalis, and Enterocytozoon bieneusi. In this study, 197 fresh fecal samples from 8 NHP species in Qinling Mountains, northwestern China, were collected and examined using multilocus sequence typing (MLST) method. The results showed that 35 (17.8%) samples were positive for tested parasites, including Cryptosporidium spp. (3.0%), G. intestinalis (2.0%), and E. bieneusi (12.7%). Cryptosporidium spp. were detected in 6 fecal samples of Macaca mulatta, and were identified as C. parvum (n=1) and C. andersoni (n=5). Subtyping analysis showed Cryptosporidium spp. belonged to the C. andersoni MLST subtype (A4, A4, A4, and A1) and C. parvum 60 kDa glycoprotein (gp60) subtype IId A15G2R1. G. intestinalis assemblage E was detected in 3 M. mulatta and 1 Saimiri sciureus. Intra-variations were observed at the triose phosphate isomerase (tpi), beta giardin (bg), and glutamate dehydrogenase (gdh) loci, with 3, 1, and 2 new subtypes found in respective locus. E. bieneusi was found in Cercopithecus neglectus (25.0%), Papio hamadrayas (16.7%), M. mulatta (16.3%), S. sciureus (10%), and Rhinopithecus roxellana (9.5%), with 5 ribosomal internal transcribed spacer (ITS) genotypes: 2 known genotypes (D and BEB6) and 3 novel genotypes (MH, XH, and BSH). These findings indicated the presence of zoonotic potential of Cryptosporidium spp. and E. bieneusi in NHPs in Qinling Mountains. This is the first report of C. andersoni in NHPs. The present study provided basic information for control of cryptosporidiosis, giardiasis, and microsporidiosis in human and animals in this area.

• IMMUNE NETWORK
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• 제21권2호
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• pp.12.1-12.17
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• 2021

Coronavirus disease 2019 (COVID-19) is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Since the emergence of SARS-CoV-2 in the human population in late 2019, it has spread on an unprecedented scale worldwide leading to the first coronavirus pandemic. SARS-CoV-2 infection results in a wide range of clinical manifestations from asymptomatic to fatal cases. Although intensive research has been undertaken to increase understanding of the complex biology of SARS-CoV-2 infection, the detailed mechanisms underpinning the severe pathogenesis and interactions between the virus and the host immune response are not well understood. Thus, the development of appropriate animal models that recapitulate human clinical manifestations and immune responses against SARS-CoV-2 is crucial. Although many animal models are currently available for the study of SARS-CoV-2 infection, each has distinct advantages and disadvantages, and some models show variable results between and within species. Thus, we aim to discuss the different animal models, including mice, hamsters, ferrets, and non-human primates, employed for SARS-CoV-2 infection studies and outline their individual strengths and limitations for use in studies aimed at increasing understanding of coronavirus pathogenesis. Moreover, a significant advantage of these animal models is that they can be tailored, providing unique options specific to the scientific goals of each researcher.