• 한국멀티미디어학회논문지
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• 제13권9호
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• pp.1285-1298
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• 2010

비선점형 태스크 스케줄링 정책을 사용하는 TinyOS는 선입선출 (FIFO: First-In First-Out) 방식의 태스크 스케줄링만 제공하기 때문에 최상위 우선순위를 가친 사용자 태스크가 즉시 실행이 필요한 태스크임에도 불구하고 우선순위가 낮은 태스크가 획득한 CPU 사용권한을 선점하지 못한다. 따라서 실시간 서비스를 요구하는 사용자 태스크 (User Task) 의 마감시한 (Deadline)을 보장할 수 없다. 또한, 비선점 환경의 TinyOS에서 사용자 태스크가 요청한 실시간 서비스를 완료하기 위해서는 사용자 태스크의 마감시한을 보장함과 동시에 사용자 태스크에서 호출 및 실행되는 TinyOS 플랫폼 태스크들의 마감시한도 보장해야 한다. 이에 본 논문에서는 비선점형 태스크 스케줄링 정책을 사용하는 기존 TinyOS 환경에서 실시간성을 제공하는 태스크 그룹 기반의 스케줄링 기법을 제안하였다. 제안한 기법은 요청한 사용자 태스크의 마감시한을 보장하기 위하여 사용자 태스크와 함께 사용자 태스크가 완료되기 위하여 호출 및 실행이 필요한 다수의 TinyOS 플랫폼 태스크를 태스크 그룹으로 형성한 후, 해당 태스크 그룹을 하나의 가상적인 큰 태스크 단위로 스케줄링한다. 제안한 기법의 동작을 시험한 결과, 제안한 기법은 비선점형 태스크 스케줄링 정책을 사용하는 TinyOS 환경에서 사용자 태스크의 마감시한을 보장함과 동시에 사용자 태스크의 평균 응답시간을 줄이고 기존 TinyOS 플랫폼간의 호환성을 제공할 수 있었다.

• 한국환경농학회지
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• 제33권2호
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• pp.121-128
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• 2014

BACKGROUND: The disease resistant (OsCK1) rice was generated by inserting choline kinase (CK1) and phosphinothricin acetyltransferase (PAT) genes isolated from Oryza sativa and Streptomyces hygroscopicus into the genome of the rice, Nakdongbyeo. With the potential problems of safeties, the evaluations on non-target organisms are essentially required for the environmental risk assessment of genetically modified (GM) crops. In the present study, we conducted the evaluation of acute toxicity on Daphnia magna that commonly used as a model organism in ecotoxicological studies for non-target organism evaluation. METHODS AND RESULTS: Effect of acute toxicity to Daphnia magna by each concentration were investigated in the disease resistant (OsCK1) rice and non-genetically modified (non-GM) rice, Nakdongbyeo, as concentration (0, 1,000, 1,800, 3,240, 5,830, 10,500 and 20,000 mg/L). The OsCK1 rice used for the test was confirmed to express the OsCK1/PAT gene by the PCR(Polymerase chain reaction) and western blot analysis. Feeding test showed that no significant differences in cumulative immobility and abnormal response of Daphnia magna fed on OsCK1 rice or non-GM rice. The 48hr-$EC_{50}$ values showed no difference between OsCK1 rice (3,147.18 mg/L) and non-GM rice (3,596.27 mg/L). CONCLUSION: This result suggested that there was no significant difference in toxicity to Daphnia magna between OsCK1 rice and non-GM counterpart.

• 한국환경농학회지
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• 제32권3호
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• pp.231-239
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• 2013

본 연구는 병저항성 OsCK1 유전자와 제초제저항성 PAT 유전자가 도입된 병저항성 GM벼에서 도입 유전자의 발현 검증과 수서환경의 비표적 생물체인 잉어와 미꾸리에 미치는 영향을 확인하기 위해 수행되었다. 병저항성 GM벼에 도입된 OsCK1와 PAT 유전자의 PCR 분석 결과, 병저항성 GM벼에서만 특이적인 밴드가 검출되었으며, PAT 단백질 발현량을 ELISA 분석한 결과, 병저항성 GM벼에서만 $45.44{\pm}2.23{\mu}g/g$ 수준으로 검출되었고, 모품종인 낙동벼에서는 검출되지 않았다. 병저항성 GM벼와 낙동벼의 미꾸리(Misgurnus anguillicaudatus)와 잉어(Cyprinus carpio)에 대한 급성독성시험을 실시한 결과, 48시간 및 96시간-$LC_{50}$ 은 5,000 mg/L 이상으로 나타났다. 48시간 및 96시간 무영향농도 NOEC)는 5,000 mg/L이었다. 급성독성 시험기간 중 병저항성 GM벼와 낙동벼간의 pH, DO, 수온, 체중 및 전장에 대한 유의적인 결과는 나타나지 않았다.

• Animal Bioscience
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• 제37권2호
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• pp.193-202
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• 2024

Objective: Oxidative stress (OS) is a pathological process arising from the excessive production of free radicals in the body. It has the potential to alter animal gene expression and cause damage to the jejunum. However, there have been few reports of changes in the expression of long noncoding RNAs (lncRNAs) in the jejunum in piglets under OS. The purpose of this research was to examine how lncRNAs in piglet jejunum change under OS. Methods: The abdominal cavities of piglets were injected with diquat (DQ) to produce OS. Raw reads were downloaded from the SRA database. RNA-seq was utilized to study the expression of lncRNAs in piglets under OS. Additionally, six randomly selected lncRNAs were verified using quantitative real-time polymerase chain reaction (qRT-PCR) to examine the mechanism of oxidative damage. Results: A total of 79 lncRNAs were differentially expressed (DE) in the treatment group compared to the negative control group. The target genes of DE lncRNAs were enriched in gene ontology (GO) terms and Kyoto encyclopedia of genes and genomes (KEGG) signaling pathways. Chemical carcinogenesis-reactive oxygen species, the Foxo signaling pathway, colorectal cancer, and the AMPK signaling pathway were all linked to OS. Conclusion: Our results demonstrated that DQ-induced OS causes differential expression of lncRNAs, laying the groundwork for future research into the processes involved in the jejunum's response to OS.

• 대한족부족관절학회지
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• 제12권1호
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• pp.55-58
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• 2008

Purpose: The purpose of this study is to find out the clinical results of excision of the Os trigonum through a posterolateral approach and to compare the surgical results of athletes with non-athletes. Materials and Methods: Within a five year and four month period, from July 2001 to October 2006, twenty patients underwent excision of symptomatic os trigonum, with a mean age of 22 years and 9 months at the time of the operation. There were fifteen female patients and five male patients. Eight were athletes and twelve were non-athletes. Results: The average duration of postoperative follow-up was thirty months. The postoperative AOFAS scored an average of 89 points compared to the preoperative AOFAS scored an average of 67 points. Sixteen patients (80%) who were operated, had good or excellent satisfactory results. The average preoperative AOFAS score of the athletes were 61 points, compared to the average postoperative AOFAS score of 90 points. For non-athletes, the average preoperative score was 71 points, compared to the average postoperative AOFAS score of 88 points. Seven athletes (87%) and nine non-athletes (75%) had good or excellent satisfaction results after surgery. The time until full recovery averaged 88 days for all the patients. 133 days for the athletes and 56 days for the non-athletes. There is no analytic difference between result in athletes and result in non-athletes. Conclusion: Open surgical treatment through posterolateral approach of os trigonum syndrome of the ankle may be effective modality regardless of the patient being an athlete or non-athlete.

• Food Science and Biotechnology
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• 제18권2호
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• pp.519-525
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• 2009

A bacterial strain (Strain N-08) capable of extracellularly producing high level of non-reducing oligosaccharide (NR-OS) isolated from soil. The strain was identified phylogenetically by 16S rDNA sequence analysis and found to be very close to Arthrobacter crystallopoietes. The high production of NR-OS was observed in the basal culture medium containing maltose as a sole carbon source. The NR-OS in culture supernatant was purified by glucoamylase treatment and Dowex-1 (OH.) ion exchange chromatography and its structure was characterized. This oligosaccharide consisted of only glucose. Methylation analysis indicated that this fraction was composed mainly of non-reducing terminal glucopyranoside. Matrixassisted laser-induced/ionization time-of-flight (MALDI-TOF) and electrospray ionization-mass spectrometry (ESI-MS)/MS analyses suggested that this oligosaccharide comprised non-reducing disaccharide unit with 1,1-glucosidic linkage. When this disaccharide was analyzed by $^1H$-NMR and $^{13}C$-NMR, it gave the same signals with $\alpha$-D-glucopyranosyl-(1,1)-$\alpha$-Dglucopyranoside. These results indicated that the NR-OS produced by A. crystallopoietes N-08 was ${\alpha}1$,${\alpha}1$-trehalose. This is the first report of the trehalose which can be produced directly from maltose by A. crystallopoietes N-08.

• 한국정보처리학회:학술대회논문집
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• 한국정보처리학회 2008년도 춘계학술발표대회
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• pp.585-588
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• 2008

센서 네트워크에 사용되는 운영체제 중 TinyOS는 Event-driven 방식이며 Component 기반의 센서 네트워크 운영체제이다. 이러한 TinyOS는 일단 태스크가 시작되면 마칠 때까지 다른 태스크가 기다려야하는 비선점형(Non-preemption) 방식이다. 최근 연구에서 TinyOS의 빠른 반응성을 위해 선점(Preemption)할 수 있는 기능이 추가되었다. 그러나 프로그래밍할 때 우선순위를 미리 주어줘야 하는 단점이 있다. 본 논문에서는 좀 더 유연하게 우선순위를 변경할 수 있는 방식을 제안하고자 한다.

• 한국정보처리학회:학술대회논문집
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• 한국정보처리학회 2009년도 춘계학술발표대회
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• pp.670-673
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• 2009

TinyOS는 현재 가장 널리 사용되는 센서 노드용 운영체제이지만, 태스크의 실시간성을 지원하지 않는다는 단점이 있다. 이에 TinyOS에 실시간성을 부여하기 위한 다양한 연구가 진행되었다. 그러나 이들 연구는 TinyOS의 사용자 태스크에 대한 실시간성만을 고려하여, TinyOS 플랫폼이 제공하는 태스크가 포함된 실제의 센서 노드 작업에 대해서는 실시간성을 만족시키지 못한다는 문제점이 있다. 따라서 본 논문에서는 TinyOS에서 센서 노드 작업의 실시간성을 지원하는 새로운 스케줄링 기법을 제안하고자 한다. 이를 위해 기존 연구의 스케줄링 기법을 센서 노드 작업에 적용했을 때 나타나는 작업 중첩 현상과 우선순위 조정 현상을 분석하고, 이를 효율적으로 해결하는 비선점형 EDF(Earliest Deadline First) 작업 스케줄링 기법을 구현하였다. 그리고 제안한 스케줄링 기법은 TinyOS의 이벤트 기반 비선점형 속성을 유지하여 제한된 하드웨어 자원을 가지는 센서 노드에 적합하다는 것을 확인하였다.

• 동의생리병리학회지
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• 제30권5호
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• pp.308-313
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• 2016

The purpose of this study is to investigate the effects of Oja-Shingiwhan(OS) in contracted corpus cavernosum smooth muscle and its mechanism. To evaluate the relaxation of OS in contracted corpus cavernosum, OS was treated in strips which were precontracted with phenylephrine(PE). To examine its mechanism, OS was treated into corporal strips contracted by PE after pretreatment of Nω-nitro-L-arginine(L-NNA) and compared with non-pretreatment of _L-NNA. In calcium chloride(Ca²⁺)-free krebs solution, Ca²⁺ 1 mM was treated into corporal strips contracted by PE after pretreatment of OS and compared with non-pretreatment of OS. action were measured by histochemical, immunohistochemical methods. OS significantly affected on the relaxation of corporal strips, and the relaxation effects were inhibited by pretreatment of L-NNA. Contractions induced by Ca²⁺ influx were inhibited by pretreatment of OS in Ca²⁺-free krebs solution. OS increased eNOS positive reaction in corpus cavernosum, but decreased PDE-5 positive reaction. These result suggest that the effect of OS in contracted corpus cavernosum smooth muscle are shown by suppressing extracellular Ca²⁺ influx and increase of eNOS, NO production and decrease of PDE-5.

• Asian Pacific Journal of Cancer Prevention
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• 제16권1호
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• pp.321-326
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• 2015

Background: The aim of this study was to explore the prognostic role of metabolic response to chemotherapy, determined by FDG-PET, in patients with metastatic non-small-cell lung cancer (NSCLC). Materials and Methods: Thirty patients with metastatic NSCLC were analyzed for prognostic factors related to overall survival (OS) and progression free survival (PFS). Disease evaluation was conducted with FDG-PET/CT and contrast-enhanced CT prior to and at the end of first-line chemotherapy. Response evaluation of 19 of 30 patients was also performed after 2-3 cycles of chemotherapy. Morphological and metabolic responses were assessed according to RECIST and PERCIST, respectively. Results: The median OS and PFS were 11 months and 6.2 months, respectively. At the end of first-line chemotherapy, 10 patients achieved metabolic and anatomic responses. Of the 19 patients who had an interim response analysis after 2-3 cycles of chemotherapy, 3 achieved an anatomic response, while 9 achieved a metabolic response. In univariate analyses, favorable prognostic factors for OS were number of cycles of first-line chemotherapy, and achieving a response to chemotherapy at completion of therapy according to the PERCIST and RECIST. The OS of patients with a metabolic response after 2-3 cycles of chemotherapy was also significantly extended. Anatomic response at interim analysis did not predict OS, probably due to few patients with anatomic response. In multivariate analyses, metabolic response after completion of therapy was an independent prognostic factor for OS. Conclusions: Metabolic response is at least as effective as anatomic response in predicting survival. Metabolic response may be an earlier predictive factor for treatment response and OS in NSCLC patients.