• Title/Summary/Keyword: Non-Formation

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Study of Soot Formation in Fuel Rich Combustion (농후 연소 추진제의 Soot 생성 특성에 관한 연구)

  • Yu, Jung-Min;Lee, Chang-Jin
    • Proceedings of the Korean Society of Propulsion Engineers Conference
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    • 2007.04a
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    • pp.143-147
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    • 2007
  • Kerosene and diesel are compounded fuels with various types of hydrocarbon elements and difficult to model the chemical kinetics. This study focuses on the prediction of the non-equilibrium reaction of fuel-rich combustion with detailed kinetics developed by Dagaut using PSR(perfectly stirred reactor) assumption. In Dagaut's surrogate model for kerosene and diesel, chemical kinetics consists of 2352 reaction steps with 298 chemical species. Also, Frenklach's soot model was implemented along with detailed kinetics to calculate the gas properties of fuel rich combustion efflux.

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Development of Basta Resistant Tobacco Using Artificial Phosphinothricin Acetyltransferase Gene (인공합성 Phosphinothricin Acetyltransferase 유전자에 의한 Basta 내성 연초식물체의 개발)

  • 양덕춘
    • Korean Journal of Plant Resources
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    • v.11 no.2
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    • pp.188-194
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    • 1998
  • This experiment was conducted to introduce phosphinothricin acetyl -transferase(PAT) gene, resistant to basta and non-selective herbidide, into tobacco(Nicotiana tabacum cv.BY4). For shoot formation,tobacco leaf disks were placed on the MS medium supplemented with 2.0mg/L BA and 0.1mg/L NAA. In this medium condition, tobacco leaf disces were cocultivated with A. tumefaciens MP90 containing NPT IIand PAT resistant to kanamycin and Basta, respectively. Shoots were obtained in the medium containing antibiotics, and those were transferred to rooting medium supplemented with 0.1mg/L NAA and antibiotics. The plants obtaining roots were transplanted into soil. Phenotype of transgenic tobacco plant was mostly as normal plant. However, about 5% was abnormal plant, which did not set seeds. PCR analysis and southern blot were performed to determine transformation. As the results, it was confirmed that PAT gene was stably integrated into tobacco genome.When herbicide, basta, was sprayed to the plants confirmed by PCR, the transgenic plants showed normal growth, whereas normal plants died. Therefore, the result of this experiment show that tobacco transformation for the resistance to basta, non-selective herbicide, was successful because PAT gene was stably integrated into tobacco.

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Synthesis of New Uraci1-5-Sulphonamide-p-Phenyl Derivatives and Their Effect on Biomphalaria alexandrina Snail's Nucleoproteins

  • Fathalla, O.A.;Gad, H.S.M.;Maghaby, A.S.
    • Archives of Pharmacal Research
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    • v.23 no.2
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    • pp.128-138
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    • 2000
  • In continuation of the previous work (Fathalla, 1992) on the synthesis of some heterocycles containing uracil moiety, we report herein the incorporation of uracil moiety into cyan-opyridine thione, thiosemecarbazone, semicarbazone, cyanopyridine, ami nocyano pyridine, isoxazoline, pyrazoline, pyrimidine, triazolo pyrimidine, pyran, selena and thiazole derivatives which might modify their biological activities. The biological studies revealed that the chemical compound III f showed high molluscicdal activity than other compounds. The profile of the nucleoprotein extracted from chemically (compound IIIc, e, f and g) treated or UV-irradiated B.alexandrina snails did not show appreciable differences when compared to non-treated (native) snails by using SDS-PAGE, where no obvious qualitative or quantitative differences were observed. Immunization of experimental animals with the nucleoprotein extracted from native, chemically (compound III f & g) treated or physically treated B.alexandrina snails induced significant protection against challenge with normal S.mansoni cercariae, as compared to the non-immunized challenged control. As well as , a decrease in the number of granuloma formation and the size range of granuloma was also observed in immunized animals. It is concluded that, compounds III f and g have a potent molluscicidal activity. They also induced chemical modification comparable to that induced by physical treatment in the snail's nucleoprotein, which could possibly be used in immunization against S. mansoni infection.

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Effect of Casing Layer on Growth Promotion of the Edible Mushroom Pleurotus ostreatus

  • Cho, Young-Sub;Weon, Hang-Yeon;Joh, Jung-Ho;Lim, Jong-Hyun;Kim, Kyung-Yun;Son, Eun-Suk;Lee, Chang-Soo;Cho, Bong-Gum
    • Mycobiology
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    • v.36 no.1
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    • pp.40-44
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    • 2008
  • Various bacteria were isolated from the casing layer soil of the culture bed of P. ostreatus and their role in fruiting body induction of the edible mushroom, P. ostreatus, was investigated. Analysis of the bacterial community isolated from the casing layer soil revealed that the composition of genera and number of cultivable bacteria were different for each sterilizing treatment. Bordetella was predominant in the bulk soil whereas Flavobacterium was predominant after sterilization of the casing layer soil. Fluorescent Pseudomonas was predominant in the non-sterilized casing layer soil. Total number of the bacterial genera in the casing layer soil was higher than that in the bulk soil. In particular, an increase in the fluorescent Pseudomonas population was observed in the non-sterilized casing layer accompanied by induction of fruiting body and enhanced mushroom production yield. The results suggested that specific bacterial populations in the casing layer play an important role in the formation of primodia and the development of basidiome in P. ostreatus.

An Experimental Study on the Non-Uniform Flow Distribution in the Windbox of an Oil-Fired Boiler (유류 연소 발전용 보일러에서 공기 공급 계통의 불균일성에 관한 실험적 연구)

  • Go, Young-Gun;Kim, Young-Zoo;Choi, Sang-Min
    • Journal of the Korean Society of Combustion
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    • v.11 no.1
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    • pp.1-10
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    • 2006
  • Oil-fired power plant usually uses several burners and combustion air is supplied to each burner through the complicated duct which is called windbox. A windbox should be designed to supply combustion air to each burner uniformly but, due to the complicated duct shape, flow distribution in the windbox is unbalanced and non-uniform supplies of combustion air are induced by these unbalanced flows in the windbox. These flow patterns tend to make flame unstable, increase the formation of pollutants and lower the overall combustion efficiency. To prevent these disadvantages, flow patterns in the windbox should be investigated for the uniform flow distribution. In this study, computational simulation method was used to investigate the flow distribution in a windbox and measured the velocities at the exit of burners in a real windbox and model tests to compare with CFD results. The results show two significant flow patterns. One is that the flow rates of each burner are different from each other and this means that all burners operate in different conditions of air to fuel ratio. The other is that the flow distribution at the exit of each burner is not axi-symmetric although the burner shape is axi-symmetric. Additionally some modifications of windbox shape and installation of baffles were proposed to make the uniform flow in the windox.

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Effect of Positioning on Back Pain and Comfort of Bed Rest Patients after Transhepatic Arterial Chemoembolization (체위변경이 간동맥 화학색전술 후 침상안정기 환자의 요통과 안위에 미치는 효과)

  • Park Han-Jong;Sohng Kyeong-Yae
    • Journal of Korean Academy of Fundamentals of Nursing
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    • v.12 no.3
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    • pp.317-324
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    • 2005
  • Purpose: The study was done to investigate whether positioning relieves the back pain and enhances comfort during bed rest after transhepatic arterial chemoembolization(TACE). Methods: A quasi-experimental design with non-equivalent control group non-synchronized design was used. The experimental group was placed semi Fowler's position for the first 2 hours. The following 4 hours the position was rotated hourly between 30 degrees laterally inclined position and a semi Fowler's position. The control group was maintained in a supine position for the 6 hours. Data were analyzed using t-test, $x^2$-test, Fisher's exact test and repeated measures ANOVA. Results: 1) Back pain intensity significantly decreased in the experimental group compared to the control group. 2) Comfort level significantly decreased in both groups, but there was no significant difference between the two groups. 3) There were no hematoma and bleeding complication in either group. In addition, there was no significant difference in urinary retention between the two groups. 4) Analgesics were less frequently taken by the experimental group. Conclusion: These results suggest that positioning relieves back pain without causing an increased incidence of hematoma and bleeding formation after TACE and this nursing intervention might help patients be more comfortable during the treatment of TACE.

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Estimation of Mechanical Properties of Concrete in Early Age by Resonance Frequency Test (공명주기식 동탄성계수를 이용한 초기재령 콘크리트의 역학적 성질 예측)

  • Kim, Jin-Keun;Kiim, Hoon;Noh, Jae-Ho
    • Magazine of the Korea Concrete Institute
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    • v.7 no.5
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    • pp.164-171
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    • 1995
  • Drying shrinkage and hydration heat are important factors on the initiation of the crack in con crete at early age. Therefore, the stress caused by hydration heat and drying shrinkage should be .analyzed to predict whether the crack occurrs or not. And, mechanical properties of early age concrete is also required for the predicting crack formation In this study, non-destructive test method of resonance frequency was used to find the relation between dynamic modulus and mechanical properties of concrete in early age. Test results were compared with existing equations, and a new equation based on test. results in this study and other data was also proposed

Study on Via hole formation in multi layer MCM-D substrate using photosensitive BCB (감광성 BCB를 사용한 다층 MCM-D 기판에서 비아홀 형성에 관한 연구)

  • 주철원;최효상;안용호;정동철;김정훈;한병성
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2000.07a
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    • pp.99-102
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    • 2000
  • Via for achieving reliable fabrication of MCM-D substrate was formed on the photosensitive BCB layer. MCM-D substrate consists of photosensitive BCB(Benzocyclobutene) interlayer dielectric and copper conductors. In order to form the vias in photosensitive BCB layer, the process of BCB and plasme etch using $C_2$F$_{6}$ gas were evaluated. The thickness of BCB after soft bake was shrunk down to 60% of the original. AES analysis was done on two vias, one is etched in $C_2$F$_{6}$ gas and the other is non etched. On via etched in $C_2$F$_{6}$, native C was detected and the amount of native C was reduced after Ar sputter. On via non etched in $C_2$F$_{6}$, organic C was detected and amount of organic C was reduced a little after Ar sputter. As a result of AES, BCB residue was not removed by Ar sputter, so plasma etch is necessary for achieving reliable via.ble via.

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Developmental Competence of Porcine NT Embryos Constructed by Microinjection of Fibroblast Cells into Vitrified Porcine Oocytes

  • Kim, Y.H.;Seok, H.B.;Kim, S.K.
    • Journal of Embryo Transfer
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    • v.22 no.4
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    • pp.265-269
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    • 2007
  • This study was conducted to investigate the efficacy of vitrification procedure for the cryopreservation of porcine oocytes and the utilization of vitrified oocytes as recipient cytoplasts for somatic cell nuclear transfer (NT), and observed that porcine oocytes are evaluated by pronuclear formation, and parthenogenetic development. Single fetal donor cells were deposited into the perivitelline space of vitrified enucleation oocytes, followed by electrical fusion and activation. NT embryos were cultured in NCSU-23 medium supplemented with 5% FBS, at $38.5^{\circ}C$ in 5% $CO_2$ and air. 1. When the developmental rates of the oocytes after being culture for $0{\sim}10$ hours vitrified with EDS and ETS were 42.0%, 38.0%, respectively. This results were lower than the control group(62.2%). 2. When the developmental rates of the oocytes after being culture for $0{\sim}10$ hours vitrified-thawed with sucrose and glucose, 5% PVP, NCSU-23 supplemented with 10% FBS were 33.3%, 25.9%, respectively. This results were lower than the control group(55.6%). 3. The fusion and development to the blastocyst stage between the NT embryos constructed with the vitrified and non-vitrified oocytes were significant differences. Developmental rate of oocytes and NT embryos constructed with the vitrified or non-vitrified oocytes were $13.0{\pm}2.4%\;and\;23.2{\pm}2.4%$, respectively.

Heterologous Production and Detection of Recombinant Directing 2-Deoxystreptamine (DOS) in the Non-Aminoglycoside-Producing Host Streptomyces venezuelae YJ003

  • Kurumbang, Nagendra Prasad;Oh, Tae-Jin;Liou, Kwangkyoung;Sohng, Jae-Kyung
    • Journal of Microbiology and Biotechnology
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    • v.18 no.5
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    • pp.866-873
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    • 2008
  • 2-Deoxystreptamine is a core aglycon that is vital to backbone formation in various aminoglycosides. This core structure can be modified to develop hybrid types of aminoglycoside antibiotics. We obtained three genes responsible for 2-deoxystreptamine production, neo7, neo6, and neo5, which encode 2-deoxy-scyllo-inosose synthase, L-glutamine: 2-deoxy-scyllo-inosose aminotransferase, and dehydrogenase, respectively, from the neomycin gene cluster. These genes were cloned into pIBR25, a Streptomyces expression vector, resulting in pNDOS. The recombinant pNDOS was transformed into a non-aminoglycoside-producing host, Streptomyces venezuelae YJ003, for heterologous expression. Based on comparisons of the retention time on LC-ESI/MS and ESI-MS data with those of the 2-deoxystreptamine standard, a compound produced by S. venezuelae YJ003/pNDOS was found to be 2-deoxystreptamine.