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In vitro and In vivo Activities of a Biocontrol Agent, Serratia plymuthica A2l-4, Against Phytophthora capsici

  • Shen, Shun-Shan;Park, Ok-Hee;Lee, Sun-Mi;Park, Chang-Seuk
    • The Plant Pathology Journal
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    • v.18 no.4
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    • pp.221-224
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    • 2002
  • In vitro and in vivo activities of a biocontrol agent, Serratia plymuthica strain A2l-4, was evaluated for the control of Phytophthora blight of pepper, Strain A2l-4 inhibited mycelial growth, germination of zoosporangia and cystospores, and formation of zoospore and zoosporangia of Phytophthora capsici in vitro. In the pot experiment, incidence of Phytophthora blight of pepper in non-treated control was 100% at 14 days after inoculation, while no disease was observed in the plot treated with S. plymuthica A2l-4. In the greenhouse test, infection rate of pepper in the non-treated plots was 74.5%, while it was only 12.6% in the plots treated with A2l-4. Results indicate that S. plymuthica A2l-4 is a potential biocontrol agent for Phytophthora blight of pepper.

Study on Boundary Lubrication in the Sliding Bearing System under High Load and Speed (고하중과 고속 미끄럼 베어링 시스템의 경계윤활에 대한 연구)

  • 장시열
    • Tribology and Lubricants
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    • v.15 no.3
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    • pp.248-256
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    • 1999
  • Many tribological components in automobile engine undergo high load and sliding speed with thin film thickness. The lubrication characteristics of the components are regarded as ether hydrodynamic lubrication or boundary lubrication, whereas in a working cycle they actually have both characteristics. Many modem engine lubricants have various additives for better performance which make boundary film formation even under hydrodynamic lubrication regime. Conventional Reynolds equation with the viewpoints of continuum mechanics concerns only bulk viscosity of lubricant, which means that its simulation does not give insights on boundary lubrication characteristics. However, many additives of modern engine lubricant provide mixed modes of boundary lubrication characteristics and hydrodynamic lubrication. Especially, high molecular weight polymeric viscosity index improvers form boundary film on the solid surface and cause non-Newtonian fluid effect of shear thinning. This study has performed the investigation about journal bearing system with the mixed concepts of boundary lubrication and hydrodynamic lubrication which happen concurrently in many engine components under the condition of viscosity index improver added.

Studies on L-Glutamic Acid-Producing Bacteria(I) (L-Glutamic Acid 생성균에 관한 연구 1)

  • 홍순우;하영칠;차승희
    • Korean Journal of Microbiology
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    • v.12 no.1
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    • pp.37-51
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    • 1974
  • Three strains which accumulated large quantities of L-hlutamic acid as the chief product of metabolism in the presence of glucose and inorhanic ammonium salt under the aerobic condition sere newly isolated. These strains have the general characters as follows: Gram-positive, short fod-shaped, non-sporulating, non-motile, and facultative anaerobe. A change of cell shates was not almost observed in their life cycles and neither a phenomenon of cell-elongation nor a pleomorphism was recognized in any cases. Together with rigid stability in Gram-stain, these characters as above indicated evidences that newly isolates would belong to genus Brevibacterium clearly differentiated from genus Corynebacterium. At the same time the newly isolates, in addition to nutritional requirement of biotin and thiamine, showed the distinctive character of requirement of special amino acid such as histidine or cysteine for their considerable growth. These physiological characters including nitrates reduction, reaction on milk, and slow formation of acid from sugar also were useful in confirming that these bacteria would be Brevibacterium ammoniagenes.

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Reliability of column capacity design in shear

  • Thomos, George C.;Trezos, Constantin G.
    • Computers and Concrete
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    • v.10 no.5
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    • pp.507-521
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    • 2012
  • The capacity design of shear forces is one of the special demands of EC8 by which the ductile behavior of structures is implemented. The aim of capacity design is the formation of plastic hinges without shear failure of the elements. This is achieved by deriving the design shear forces from equilibrium conditions, assuming that plastic hinges, with their possible over-strengths, have been formed in the adjacent joints of the elements. In this equilibrium situation, the parameters (dimensions, material properties, axial forces etc) are random variables. Therefore, the capacity design of shear forces is associated with a probability of non-compliance (probability of failure). In the present study the probability of non-compliance of the shear capacity design in columns is calculated by assuming the basic variables as random variables. Parameters affecting this probability are examined and a modification of the capacity design is proposed, in order to achieve uniformity of the safety level.

Reliability based calibration of the capacity design rule of reinforced concrete beam-column joints

  • Thomos, George C.;Trezos, Constantin G.
    • Computers and Concrete
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    • v.8 no.6
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    • pp.631-645
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    • 2011
  • The capacity design rule for beam-column joints, as adopted by the EC8, forces the formation of the plastic hinges to be developed in beams rather than in columns. This is achieved by deriving the design moments of the columns of a joint from equilibrium conditions, assuming that plastic hinges with their possible overstrengths have been developed in the adjacent beams of the joint. In this equilibrium the parameters (dimensions, material properties, axial forces etc) are, in general, random variables. Hence, the capacity design is associated with a probability of non-compliance (probability of failure). In the present study the probability of non-compliance of the capacity design rule of joints is being calculated by assuming the basic variables as random variables. Parameters affecting this probability are examined and a modification of the capacity design rule for beam-column joints is proposed, in order to achieve uniformity of the safety level.

Linear and Hyperbranched Polymers via Electrophilic Substitution Reaction in Polyphosphoric $Acid/P_{2}O_{5}$

  • Choi, Ja-Young;Jeon, In-Yeop;Tan, Loon-Seng;Baek, Jong-Beom
    • Proceedings of the Polymer Society of Korea Conference
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    • 2006.10a
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    • pp.195-195
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    • 2006
  • A superior electrophilic substitution reaction medium that is non-toxic, relatively less corrosive, and non-volatile electrophilic substitution reaction to afford high molecular weight linear and hyperbranched polyetherketones (PEK' s) was developed. The system has very strong driving force to give extra ordinary high molecular weight linear and hyperbranched PEK' s. The reaction medium was further extended to prepare various types of copolymers and covalently grafted polymers onto carbon nanotube (CNT) or carbon nanofiber (CNF). By using characteristic hydrophilic nature of the reaction medium, hyperbranched PEK' s could be synthesized from commercially available $A_3\;+\;B_2$ monomers without network formation via selective solubility of the monomers.

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Molecular Weight Distribution of Pullulan and Degrading Enzyme Activity of Aureobasidium pullulans

  • Lee, Ji-Hyeon;Kim, Mi-Ryeong;Kim, Jeong-Hwa;Lee, Jin-U;Kim, Seong-Gu
    • 한국생물공학회:학술대회논문집
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    • 2000.11a
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    • pp.325-328
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    • 2000
  • The effects of DO and pH on the mass production of pullulan with high-molecular weight from A. pullulans ATCC 42023 were evaluated. The maximum pullulan production yield (51%) was obtained at pH non control (initial pH 6.5) and DO control (above 50%) condition. The pullulan degrading enzyme was activated when the pH of broth reached lower than 5.0 and portion of low molecular weight pullulan was increased. The formation of a black pigment was observed at the initial stationary phase, 40hr of fermentation. Therefore, the fermentation should be carried out in pH non control and DO control condition and harvested before reaching stationary phase around 40h for the production of high molecular weight pullulan.

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Evaluation of particulate removal in slow sand filtration processes (완속여과 공정에서 전처리 공정 도입에 따른 입자제거 효율평가)

  • Kim, Seong-Su;Bae, Chul-Ho;Park, No-Suk;Kang, Suk-Hyung
    • Journal of Korean Society of Water and Wastewater
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    • v.22 no.4
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    • pp.461-466
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    • 2008
  • Because of their simplicity, efficiency, and economy, slow sand filters are appropriate means of water treatment for small water systems. In this study, the effect of filtration velocity and dirty skin (Schmutzdecke) was evaluated on the performance of turbidity removal. Also, removal characteristics of particulate were investigated in the case of the usage of non-woven fabric on the surface of sand and the application of PCF as pretreatment process. Comparative column tests were carried out for the various operation condition. From the result of column tests, filtration velocity had little effect on the turbidity removal rate. The formation of algal biofilm on the surface of media is helpful in turbidity removal, while non-woven fabric is not as effective as expected. The relative contribution of biomass and accumulated particulates to head loss development in slow sand filters requires further study.

Spreading Kinetics of Poly(diisobutylene maleic acid) at the Air-water Interface

  • Kim, Nam Jeong
    • Journal of the Korean Applied Science and Technology
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    • v.32 no.4
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    • pp.661-668
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    • 2015
  • The surface rheological properties of polymer monolayer show complicated non-linear viscoelastic flow phenomena when they are subjected to spreading flow. These spreading flow properties are controlled by the characteristics of flow units. The kinetics of the formation of an interfacial film obtained after spreading poly(diisobutylene maleic acid) at air-water interface were studied by measuring of the surface pressure with time. The experimental data were analyzed theoretically according to a nonlinear surface viscoelastic model. The values of dynamic modulus, static modulus, surface viscosities and rheological parameters in various area/ monomer were obtained by appling experimental data to the equation of nonlinear surface viscoelastic model.

NUMERICAL ANALYSIS OF MULTIPHASE FLOW BY NUFLEX (NUFLEX의 다상유동 해석)

  • Son, Gi-Hun;Suh, Young-Ho;YU, Tae-Jin;Hur, Nahm-Keon
    • 한국전산유체공학회:학술대회논문집
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    • 2007.04a
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    • pp.95-98
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    • 2007
  • A general purpose program NUFLEX has been extended for two-phase flows with topologically complex interface and cavitation flows with liquid-vapor phase change caused by large pressure drop. In analysis of two-phase flow, the phase interfaces are tracked by employing a LS(Level Set) method. Compared with the VOF(Volume-of-Fluid} method based on a non-smooth volume-fraction function, the LS method can calculate an interfacial curvature more accurately by using a smooth distance function. Also, it is quite straightforward to implement for 3-D irregular meshes compared with the VOF method requiring much more complicated geometric calculations. Also, the cavitation process is computed by including the effects of evaporation and condensation for bubble formation and collapse as well as turbulence in flows. The volume-faction and continuity equations are adapted for cavitation models with phase change. The LS and cavitation formulation are implemented into a general purpose program for 3-D flows and verified through several test problems.

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