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Effects of Non-Alcoholic Fatty Liver in Rats by Acer tagmentosum Maxim. Extract (산겨릅나무 추출물을 이용한 비알콜성 지방간 개선 효과)

  • Seo, Yoonhee;Lee, Sang-Hwa;Hwang, Hyung Seo;Choe, Soo Young
    • The Korean Journal of Food And Nutrition
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    • v.29 no.3
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    • pp.307-312
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    • 2016
  • This study was performed to investigate the effects of Acer tegmentosum Maxim. extract (ATE) on non-alcoholic fatty liver in Sprague Dawley (SD) rats. During oral administration of ATE, non-alcoholic fatty liver was induced by treatment with DL-ethionine. The lipid, total cholesterol (T-CHO) and malondialdehyde (MDA) in the liver tissue of ATE-fed rats showed lower levels, as compared to ATE-unfed rats. In ATE-fed rats, the serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and ${\gamma}$-glutamyl transferase (GGT) were lower than the case of ATE-unfed rats. Oil red staining of the liver showed that the lipid deposits were decreased by feeding ATE. These results strongly indicated that ATE has positive effects of protection against non-alcoholic fatty liver formation.

Splinted and non-splinted implant-supported restorations : prosthetic considerations for restoring multiple adjacent teeth (Splinted or Non-splinted: 다수의 인접한 치아 결손부 수복을 위한 임플란트 보철)

  • Yoon, Hyung-In
    • The Journal of the Korean dental association
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    • v.54 no.3
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    • pp.198-205
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    • 2016
  • The purpose of this paper was to investigate the significance of splinted and non-splinted implant-supported restorations with an internal connection for multiple consecutively missing teeth. Upon examination of the effects of fixture-abutment connection, the distribution of occlusal load was favorable in splinted implant-prosthesis with an external connection, but effect of strain distribution was not significant in splinted implant-prosthesis with an internal connection. In splinted implant-prostheses for short implants, strain distribution was not affected by the method of retention. For cement-retained prostheses, the effect of strain distribution due to splinting was not significant. In clinical studies, non-splinted prostheses with an internal connection for multiple consecutively missing teeth showed high survival rate, mild marginal bone loss, and stable periodontal condition. However, failure to achieve optimal proximal contact between single-unit prostheses may lead to food impaction, and veneer fracture may be inevitable when the framework provides inadequate support in the proximal region. In conclusion, splinted implant-prosthesis is not an indication in all cases, and clinical consideration of its use should be based on the patient's oral condition, such as location and number of implants, formation of proximal contact, canine guidance, existence of parafunctional habit, and oral hygiene, when multiple consecutively missing teeth are replaced by internal connection type implant.

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Developmental competence of in vitro-matured human oocytes obtained from pregnant and non-pregnant women

  • Pongsuthirak, Pallop;Vutyavanich, Teraporn
    • Clinical and Experimental Reproductive Medicine
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    • v.45 no.4
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    • pp.189-194
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    • 2018
  • Objective: The aim of this study was to compare the rate of maturation, fertilization, and embryo development of in vitro-matured human oocytes derived from pregnant and non-pregnant women. Methods: Immature oocytes were obtained by needle aspiration from 49 pregnant women (group 1) who underwent a cesarean section at term and 77 non-pregnant women (group 2) who underwent a gynecological operation during the same period (8 months). Healthy immature oocytes (530 in group 1 and 539 in group 2) were cultured and assessed for maturation 36 hours later. Mature oocytes were inseminated by intracytoplasmic sperm injection and cultured up to 144 hours. Results: The percentage of degenerated oocytes was significantly higher (12.1% vs. 6.3%; p<0.001) in group 1 than in group 2. There was no significant difference in the maturation rate (66.8% vs. 68.1%; p=0.698), fertilization rate (66.7% vs. 67.6%; p=0.857), or the rate of formation of good-quality blastocysts (46.2% vs. 47.2%; p=0.898) in oocytes obtained from pregnant and non-pregnant women. Conclusion: The developmental competence of immature oocytes did not differ between pregnant and non-pregnant women.

Fabrication of Nonconductive Microscale Patterns on Ion Exchange Membrane by Laser Process (레이저 가공을 이용한 이온교환막 표면의 비전도성 마이크로 패턴의 제작)

  • Jinwoong Choi;Myeonghyeon Cho; Bumjoo Kim
    • Korean Journal of Materials Research
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    • v.33 no.2
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    • pp.71-76
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    • 2023
  • The electroconvection generated on the surface of an ion exchange membrane (IEM) is closely related to the electrical/chemical characteristics or topology of the IEM. In particular, when non-conductive regions are mixed on the surface of the IEM, it can have a great influence on the transfer of ions and the formation of nonlinear electroconvective vortices, so more theoretical and experimental studies are necessary. Here, we present a novel method for creating microscale non-conductive patterns on the IEM surface by laser ablation, and successfully visualize microscale vortices on the surface modified IEM. Microscale (~300 ㎛) patterns were fabricated by applying UV nanosecond laser processing to the non-conductive film, and were transferred to the surface of the IEM. In addition, UV nanosecond laser process parameters were investigated for obvious micro-pattern production, and operating conditions were optimized, such as minimizing the heat-affected zone. Through this study, we found that non-conductive patterns on the IEM surface could affect the generation and growth of electroconvective vortices. The experimental results provided in our study are expected to be a good reference for research related to the surface modification of IEMs, and are expected to be helpful for new engineering applications of electroconvective vortices using a non-conductive patterned IEM.

Effect of bone graft materials on bone formation in guided bone regeneration using perforated titanium membrane (천공형 티타늄막을 이용한 골유도재생술 시 수종의 골이식재가 골재생에 미치는 영향)

  • Hong, Seung-Bum;Kwon, Young-Hyuk;Park, Joon-Bong;Herr, Yeek;Chung, Jong-Hyuk
    • Journal of Periodontal and Implant Science
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    • v.36 no.1
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    • pp.223-237
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    • 2006
  • The purpose of the present study was to evaluate the effect of bone graft materials including deproteinized bovine bone(DBB), demineralized freeze-dried bone(DFDB), freeze-dried bone(FDB) on bone formation in guided bone regeneration using perforated titanium membrane(TM). 16 adult male rabbits(mean BW 2kg) were used in this study and 4 rabbits allotted to each test group. Intramarrow penetration(diameter 6.5mm) was done with round carbide bur on calvaria to promote blood supply and clot formation in the wound area. The test groups were devided into 4 groups as follows: TM only(test 1), TM +DBB(test 2), TM +DFDB(test 3), TM +FDB(test 4). Perforated titanium membrane was contoured in rectangular parallelepiped shape(0.5mm pore diameter, 10mm in one side, 2mm in inner height), filled the each graft material and placed on the decorticated carvaria. Perforated titanium membrane was fixed with resorbable suture materials. The animals were sacrificed at 2, 8 weeks after the surgery. Non-decalcified preparations were routinely processed for histologic analysis. The results of this study were as follows: 1. Perforated titanium membrane was biocompatible. 2. Perforated titanium membrane had capability of maintaining the space during the healing period but invasion of soft tissue through the perforations of titanium membrane decreased the space available for bone formation. 3. In test 1 group without bone graft material, the amount of bone formation and bone maturation was better than other test groups. 4. Among the graft materials, the effect of freeze-dried bone on bone formation was best. 5. In the test groups using deproteinized bovine bone, demineralized freeze-dried bone, bone formation was a little. The spacemaking capability of the membrane may be crucial for bone formation. The combined treatment with the perforated titanium membrane and deproteinized bovine bone or demineralized freeze-dried bone failed to demonstrate any added effect in the bone formation. Minimization of size and numbers of perforations of titanium membrane or use of occlusive titanium membrane might be effective to acquire predictable results in the vertical bone formation.

The Effects of Platelet-Rich Plasma on Regeneration around Dental Implant Defects (혈소판 농축혈장이 임플랜트 주위 골결손부 재생에 미치는 영향)

  • Hong, Ki-Seok;Lim, Sung-Bin;Chung, Chin-Hyung;Lee, Chong-Heon
    • Journal of Periodontal and Implant Science
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    • v.33 no.4
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    • pp.673-691
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    • 2003
  • The current interest in periodontal tissue regeneration has lead to research in bone graft, root surface treatments, guided-tissue regeneration, and the administration of growth factors as possible means of regenerating lost periodontal tissue. Several studies have shown that a strong correlation between platelet-rich plasma and the stimulation of remodeling and remineralization of grafted bone exists, resulting in a possible increase of 15-30% in the density of bone trabeculae. The purpose of this study was to study the histopathological correlation between the use of platelet-rich plasma and a bone xenograft used in conjunction with a non-resorbable guided-tissue membrane, e-PTFE, compared to a control group with regards to bone regeneration at the implant fixture site. Implant fixtures were inserted and graft materials placed into the left femur of in the experimental group, while the control group received only implant fixtures. In the first experimental group, platelet-rich plasma and BBP xenograft were placed at the implant fixture site, and the second experimental group had platelet-rich plasma, BBP xenograft, and the e-PTFE membrane placed at the fixture site. The degree of bone regeneration adjacent to the implant fixture was observed and compared histopathologically at 2 , 4, and 8 weeks after implant fixture insertion. The results of the experiment are as follows: 1. The rate of osseointegration to the fixture threads was found to be greater in the first experimental group compared to the control group. 2. The histopathological findings of the second experimental group showed rapid resorption of BBP with subsequent new bone formation replacing the resorbed BBP. 3. The second experimental group showed new bone formation in the area adjacent to the fixture threads beginning two weeks after fixture implantation, with continued bone remodeling in the areas mesial and distal to the fixture. 4. Significant new bone formation and bone remodeling was observed in both experimental groups near the implant fixture sites. 5. The rate of osseointegration at the fixture threads was greater in the second experimental group compared to the first group, and the formation of new bone and trabeculae around the fixture site occurred after the fourth week in the second experimental group. The results of the experiment suggest that a greater degree of new bone formation and osseointegration can occur at the implant fixture site by utilizing platelet-rich plasma and bone xenografts, and that these effects can be accelerated and enhanced by concurrent use of a non-resorbable guided tissue membrane.

The Generative Mechanism of Cloud Streets

  • Sung-Dae Kang;Fujio Kimura
    • Journal of Environmental Science International
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    • v.1 no.2
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    • pp.119-124
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    • 1992
  • Cloud streets were successfully simulated by numerical model (RAMS) including an Isolated mountain near the coast, large sensible heat flux from the sea surface, uniform stratification and wind velocity with low Froude number (0.25) in the inflow boundary The well developed cloud streets between a pair of convective rolls are simulated at a level of 1 km over the sea. The following five results were obtained: 1) port the formation of the pair of convective rolls, both strong static instability and a topographically induced mechanical disturbance are strongly required at the same time. 2) Strong sensible heat flux from the sea surface is the main energy source of the pair of convective rolls, and the buoyancy caused by condensation in the cloud is negligibly small. 3) The pair o( convective rolls is a complex of two sub-rolls. One is the outer roll, which has a large radius, but weak circulation, and the other is the inner roll, which has a small radius, but strong circulation. The outer roll gathers a large amount of moisture by convergence in the lower marine boundary, and the inner roll transfers the convergent moisture to the upper boundary layer by strong upward motion between them. 4) The pair of inner rolls form the line-shaped cloud streets, and keep them narrow along the center-line of the domain. 5) Both by non-hydrostatic and by hydrostatic assumptions, cloud streets can be simulated. In our case, non-hydrostatic processes enhanced somewhat the formation of cloud streets. The horizontal size of the topography does not seem to be restricted to within the small scale where non-hydrostatic effects are important.

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Consumer-brand Relationship Quality Formation of Potential Consumers for an Automotive Brand: Effectiveness of Native Advertising (자동차 브랜드 잠재소비자의 소비자-브랜드 관계 품질 형성에 대한 연구: 네이티브 광고의 효과를 중심으로)

  • Kim, Yu Seung;Choi, Hyung-Min
    • The Journal of the Korea Contents Association
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    • v.17 no.2
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    • pp.656-677
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    • 2017
  • This study aims to examine the effects of native advertising on consumer-brand relationship quality (BRQ) formation of the potential consumers in the context of an automotive brand. Specifically, the study verifies (1) whether the respondents cognize the content of native advertisement as an advertisement and (2) how the value dimensions of native advertising impact BRQ through brand attitude (BAT). To fulfill the purposes, the researchers categorized three types of advertisements (product, brand and non-commercial) and then showed three manipulated stimuli to potential consumers in the automotive market before measuring. The findings drawn from the 345 valid cases are as following. First, the rate of respondents' advertising recognition is low in the non-commercial advertisement, while it is high in ones of product and brand. Second, most of the respondents perceive less irritation toward the native ads, especially toward the non-commercial advertisement. Third, the entertainment and informativeness of native advertising positively influence BRQ, while the effect of irritation on BRQ is negative. Also, the effects of advertising value dimensions on BRQ are all mediated by BAT.

Nitrogen fixation by Rhizobium-Plant cell cultures (식물배양세포(植物培養細胞)-Rhizobium에 의(依)한 질소고정(窒素固定)에 관한 연구)

  • Park, W.C.;Yatazawa, M.H.
    • Korean Journal of Soil Science and Fertilizer
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    • v.12 no.1
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    • pp.43-46
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    • 1979
  • Curing the studies on the mechanism of the nodule formation and the infection of rhizobia using the continously cultured plant cell tissues, it was found that some calluses possess high nitrogen fixation activity. This experiment was conducted to know the differences among the calluses and the Rhizobia. The results obtained were as follows; 1) In a single inoculation the nitrogenase activity of soybean cell cultures-rhizobium was moderately higher than non-leguminous cell cultures, however, in the mixing inoculation that was reversely found. 2) Host factor, which was characteristics for the nodule formation and the nitrogenase activity, was not appeared generally in the plant cell cultures except for Kuamusume and Toyozuzu in soybean, and Datura in non-leguminous cell cultures. 3) In the 012 rhizoblium cultured on soybean cell cultures and in the 010, 023 and 024 rhizobia cultured on non-leguminous cell cultures the nitrogenase activity higher than the others.

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Subcellular Responses in Nonhost Plant Infected with Pathogenic and Non-pathogenic Strains of Xanthomonas axonopodis pv. glycines

  • Jeong, Yong-Ho;Kim, Jung-Gun;Chang, Sung-Pae;Hwang, In-Gyu;Kim, Young-Ho
    • The Plant Pathology Journal
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    • v.18 no.3
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    • pp.115-120
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    • 2002
  • Xanthomonas axonopodis pv. glycines, the causal agent of bacterial pustule of soybean, induces hypersensitive response (HR) in a non-host plant, hot pepper (Capsicum annuum). A wild-type strain (8ra) and its non-patho-genic mutant (8-13) of X. axonopodis pv. glycines were inoculated into the pepper leaf tissues and their subcellular responses to the bacterial infections were examined by electron microscopy. Intrastructural changes related to HR were found in the leaf tissues infected with 8ra from 8 h after inoculation, characterized by separation of plasmalemma from the cell wall, formation of small vacuoles and vesicles, formation of cell wall apposition, and cellular necrosis. No such responses were observed in the tissues infected with the mutant. In 8ra, the bacterial cells were attached to the cell walls, with the cell wall material dissolved into and appearing to encapsulate the bacterial cells. The bacterial cells later became entirely embedded in the cell wall material. On the other hand, in 8-13, the bacterial cells were usually not attached tightly to the plant cell wall, and no or poor encapsulation of the bacteria by the wall material occurred, although these were encircled by rather loose wall materials at the later stages.