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Microspore-derived Embryo Formation and Morphological Changes during the Isolated Microspore Culture of Radish (Raphanus sativus L.)

  • Han, NaRae;Kim, Sung Un;Park, Han Young;Na, Haeyoung
    • Horticultural Science & Technology
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    • v.32 no.3
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    • pp.382-389
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    • 2014
  • Raphanus sativus L. cv. Taebaek, a efficiently microspore-derived embryo (MDE)-forming cultivar, and 'Chungwoon', a non-MDE-forming cultivar were selected as donor plants for isolated microspore culture. Radish flower bud of 2.0 (small, S), 4.0 (medium, M), and 6.0 (large, L) ${\pm}$ 0.5 mm in length were isolated to determine the temporal relationship between flower bud size and MED yield. Anatomical observations revealed no difference in the structure of the flower buds between the two cultivars. In both cultivars, the stigmas were much longer than the floral leaf in M-sized flower buds. The MDE yields for 'Taebaek' per petri dish were 6.6 and 1.3 for M- and L-sized of flower buds, respectively, but MDE formation was not induced in the S flower buds. On the other hand, 'Chungwoon' failed to form MDEs in all flower buds. The microspore density of 'Taebaek' was 1.3 times more than that of 'Chungwoon' for M sized flower buds. Of the M-sized buds from 'Taebaek' and 'Chungwoon', 92.1 and 81.6%, respectively, were in the late uninucleate microspore stage, which is characterized by the highest frequency of MDE formation. Anatomical observations of MDE formation revealed that the microspores were able to divide to form a primordium from which cell division took place continuously in the 'Teabeak' cultivar. However, the microspores of 'Chungwoon' failed to progress beyond the primodium stage, resulting in lack of MDE formation. By contrast, after the formation of the primordium, various developmental stages of embyos from microspore were observed in the 'Taebaek' cultivar. These results can be used to determine MDE forming potentials of radish cultivars.

Determination of ethyl carbamate in maesil wine by alcohol content and ratio of maesil (Prunus mume) during ripening period (알코올 농도 및 담금비에 따른 숙성 기간별 매실주의 에틸카바메이트 함량조사)

  • Kim, Nan-Young;Eom, Mi-Na;Do, Young-Sook;Kim, Jung-Beom;Kang, Suk-Ho;Yoon, Mi-Hye;Lee, Jong-Bok
    • Food Science and Preservation
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    • v.20 no.3
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    • pp.429-434
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    • 2013
  • This study was designed to investigate the formation of ethyl carbamate (EC) during the ripening of Maesil with sugar and Soju (19.5~35% alcohol contents) using a homemade method. Maesil, sugar and Soju were purchased at ordinary market in June of 2012. The preparation of sample for analysis was conducted by method of Henry et al. The analysis of GC/MS was used SIM mode (m/z 89, 74, 62). Quantification was performed in terms of the 62 ion and was based on an internal standard procedure. Good linearity was obtained with a regression coefficient ($r^2$ = 0.993). Low detection limits (LOD) was achieved 4.31 ug/kg and recovery for alcohol was 74.8%. During 90 days, fermentation with sugar was not detected EC (under LOQ). 15 days ripened Maesil wine contained EC between non detected~32.7 ug/kg and 90 days ripened Maesil wine was 19.7~87.4 ug/kg. Higher proportion of Maesil and Soju increased EC contents. EC levels were increased 32.7 ug/kg to 87.4 ug/kg in the ratio of Maesil to 35% alcohol-Soju (1:1). In the ratio of Maesil to 35% alcohol-Soju (1:3) was increased non detected to 69.7 ug/kg. After 90 days, Maesil wine was filtered Maesil through a seive and ripened by 180 days to investigate the formation of EC compared with non filtered. Treatment of filtered, EC contents was much higher level compared with non filtered. Therefore, this result showed that alcohol contents contribute to increase EC formation more than Maesil.

Effects of Chlorine Residual and Pipe Material on the Biofilm Formation in Drinking Water Distribution Pipe (수도관의 생물막 형성에 미치는 잔류염소와 파이프 재질의 영향)

  • Park, Se-keun;Park, Jae-Woo;Sung, Kwon-Shic;Choi, Sung-Chan;Kim, Yeong-Kwan
    • Journal of Industrial Technology
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    • v.21 no.B
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    • pp.11-20
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    • 2001
  • This laboratory study examined the impact of free chlorine residual and pipe material on the formation of biofilm in drinking water distribution pipe surfaces. Result of heterotrophic plate counts(HPC) of the biofilm in the tap water-supplied reactor averaged $2.17{\times}10^5CFU/cm^2$ on PVC and $2.43{\times}10^5CFU/cm^2$ on STS 316, respectively. HPCs on the surface exposed to the tap water containing 0.2mg/L of free chlorinne residual averaged $4.24{\times}10^4CFU/cm^2$ on PVC and $6.54{\times}10^4CFU/cm^2$ on STS 316, respectively. Average of HPC/Total direct counts in the tap water-supplied reactor ranged from 1.08%(PVC) to 1.26%(STS 316) and from 0.38%(PVC) to 0.65%(STS 316) in the reactor supplemented with disinfectant, respectively. No correlation was observed between disinfectant addition and biofilm density. With regard to the biofilm formation, little difference existed between PVC and STS 316. Yellow and red pigmented bacteria were the dominant expressions in bulk fluid, whereas non-pigmented bacteria were found dominant in the biofilm. Pink/red pigmented bacteria were found to be facultative anaerobic, while yellow pigmented bacteria and non-pigmented bacteria were found to be obligate aerobic.

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A Study on the Role of -SO3- Ions in the Dehydration Limit of Poly(styrene-co-styrenesulfonic acid) Membrane

  • Ko, Kwang-Hwan;Kim, Joon-Seop;Lee, Chang Hoon
    • Elastomers and Composites
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    • v.52 no.3
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    • pp.180-186
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    • 2017
  • In this work, the effect of low-temperature dehydration of a poly(styrene-co-styrenesulfonic acid) (PSSA) membrane was investigated by differential scanning calorimetry, fourier transform infrared spectroscopy (FT-IR), electron magnetic resonancespectroscopy (EMR), and $^1H$- and $^{13}C$ solid-state nuclear magnetic resonance spectroscopy. These analyses were performed at room temperature for powdered PSSA specimens with and without dehydration and the following key observations were made. First, FT-IR analysis showed that low-temperature dehydration not only transformed the [${SO_3}^-{\cdots}H^+$] ionic pair in the non-hydrated PSSA to an $SO_3H$ group, but also induced the formation of -C=C- double bonds in the dehydrated PSSA. Second, the ${-SO_3}^{\bullet}$ radical was unambiguously identified by EMR spectroscopy. Third, H-abstraction was detected by $^1H$ magic-angle spinning spectroscopy. Finally, an unexpected color shift from white for the non-hydrated PSSA to a yellowish brown for the dehydrated sample was observed. In order to explain these experimental results, it was proposed that the formation of the intermediate hydrogen ($H^{\bullet}$) or hydroxyl radical ($HO^{\bullet}$) species was initiated by the dehydration process. The sespecies attacked the $SO_3H$ group and the tertiary proton at the ${\alpha}-carbon$, resulting in the formation of $-SO^{\bullet}$ radicals and -C=C- double bonds, which correlated with the color shift in the dehydrated PSSA sample. The semechanisms are useful for understanding the simultaneous loss of an aromatic ring and -SO- groups in the PSSA fuel cell membrane.

Effects of Light and Media on Pycnidial Formation of Didymella bryoniae (Auersw.) Rehm (병자곡 및 자양곡형성에 미치는 광선 및 배지의 영향)

  • Lee D. H.
    • Korean journal of applied entomology
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    • v.16 no.4 s.33
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    • pp.211-215
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    • 1977
  • The Sporulation of Didymella bryomiae were observed under diurnal cycles of light/darkness of near ultraviolet light (NUV) and artificial daylight (ADL) and continous darkness in eight isolates growing on PDA and V-8 juice agar. Light stimulated pycindial and perithecial formation of this fungus on potato dextrose agar and V-8 juice agar. Sprulation was poor in darkness, but some isolates were able to produce pycnidia and perithecia in the absence of light. Perithecial formation was much better under artificial daylight (ADL) on V-8 juice agar than those grown under near ultraviolet light (NUV). In general, cultures grown on V-8 juice agar sporulated better than cultures grown on PDA under three setsof light condition. Most of the pycnidiospores obtained from each isolates of this fungus grown on PDA were non-septate and microtype, but macrotype of non-septate and uniseptate pycnidiospores were produced on V-8 juice agar. Pycnidiospore produced on V-8 juice agar were similar to those produced on the radicle of naturally infected seeds. The appearance of perithecia were quite distinctive from pycnidia. The mature perithecia were darker than pycnidia and whitish spore masses formed on the ostiole of perithecia.

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The Characteristics of Ozone Formation from a Gaseous Fueled SI Engine with Various Operating Parameters (여러 가지 운전조건에 따른 가스연료엔진 오존발생량 연구)

  • 김창업;강건용;배충식
    • Transactions of the Korean Society of Automotive Engineers
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    • v.11 no.6
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    • pp.86-92
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    • 2003
  • To analyze the characteristics of ozone formation, measurements of the concentrations of individual exhaust hydrocarbon species have been made under various engine operating parameters in a 2-liter 4-cylinder engine for natural gas and LPG. Tests were performed at constant engine speed, 1800 rpm for two compression ratios of 8.6 and 10.6, with various operating parameters, such as excess air ratio of 1.0~1.6, bmep of 250~800 na and spark timing of BTDC 10~$55^{\circ}$. It was found that the natural gas gave the less ozone formation than LPG in various operating conditions. This was accomplished by reducing the emissions of propylene($C_3H_6$), which has relatively high maximum incremental reactivity factor, and propane($C_3H_8$) that originally has large portion of LPG. In addition, the natural gas show lower values in the specific reactivity and brake specific reactivity. Higher compression ratio of the test engine showed higher non methane HC emissions. However, specific reactivity value decreased since fuel species of HC emissions increase. brake specific reactivity showed almost same values under high bmep, over 500kPa for both fuels. This means that the increase of non methane HC emissions and the decrease of specific reactivity with higher bmep affect each other simultaneously. With advanced spark timing, brake specific reactivity values of LPG were increased while those of natural gas showed almost constant values.

Effects of Heavy Metal Contamination from an Abandoned Mine on Tomato Growth and Root-knot Nematode Development

  • Park, Byeong-Yong;Lee, Jae-Kook;Ro, Hee-Myong;Kim, Young-Ho
    • The Plant Pathology Journal
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    • v.27 no.3
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    • pp.266-271
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    • 2011
  • Physicochemical characteristics and heavy metal content of soils located along the drainage way of an abandoned mine at Busan, Korea ($35^{\circ}31'N$, $129^{\circ}22'E$) (contaminated soil; CS) and uncontaminated soils (50-70 m apart from the drainage way) (NS) were examined. Growth of tomato plants (Solanum lycopersicom cv. Rutgers) in CS and NS, development of the root-knot nematode (Meloidogyne incognita) as root-knot gall formation on tomato plants, and non-parasitic nematode populations in soil were also examined. Growth of tomato plants, root-knot gall formation, and non-parasitic nematode populations were significantly reduced in CS with higher As content, lower pH, higher electrical conductivity (EC), and lower available phosphate (av. $P_2O_5$) than in NS. None of the other physicochemical characters examined differed significantly between CS and NS (low and no significance) and were above or below the critical levels detrimental to plant growth and nematode development, suggesting that As may be the primary hazardous heavy metal in CS. The toxicity of As might be enhanced at low pH in CS because exchangeable forms of some heavy metals increase with the decrease of soil pH. The heavy metals, especially As, may have contributed to increasing EC and decreasing av. $P_2O_5$. Therefore, the effects of mine drainage contamination from the abandoned mine were derived primarily from contamination by heavy metals such as As. These may have been enhanced in toxicity (solubility) by the lowered pH, increased soil salinity (EC) and decreased av. $P_2O_5$. Our results suggest synergistic adverse effects on the plant and the nematode by decreasing osmotic potential and nutrient availability.

Efficient Flooding in Ad hoc Networks using Cluster Formation based on Link Density (애드 혹 네트워크에서 링크밀도기반 클러스터 구축을 이용한 효율적인 플러딩)

  • Lee, Jae-Hyun;Kwon, Kyung-Hee
    • The KIPS Transactions:PartC
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    • v.14C no.7
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    • pp.589-596
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    • 2007
  • Although flooding has the disadvantages like a transmission of duplicated packets and a packet collision, it has been used frequently to find a path between a source and a sink node in a wireless ad hoc network. Clustering is one of the techniques that have been proposed to overcome those disadvantages. In this paper, we propose a new flooding mechanism in ad hoc networks using cluster formation based on the link density which means the number of neighbors within a node's radio reach. To reduce traffic overhead in the cluster is to make the number of non-flooding nodes as large as possible. Therefore, a node with the most links in a cluster will be elected as cluster header. This method will reduce the network traffic overhead with a reliable network performance. Simulation results using NS2 show that cluster formation based on the link density can reduce redundant flooding without loss of network performance.

Lockeia gigantus ichnosp. nov. in the Lacustrine Deposits of the Early Cretaceous Jinju Formation, Southern Coast of Korea (남해안 전기 백악기 진주층의 호성 퇴적층에서 산출된 Lockeia gigantus ichnosp. nov.)

  • Kim, Kyung-Soo;Kim, Jeong-Yul
    • Journal of the Korean earth science society
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    • v.29 no.1
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    • pp.13-28
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    • 2008
  • About 450 specimens of Lockeia were discovered from the lacustrine siltstone of the Early Cretaceous Jinju Formation of Jin Island, southern coast of Korea. They are very elongated, seed-shaped Lockeia characterized by a large size, mostly 60-70 mm long and 15-20 mm wide. They are characteristically sharp longitudinal furrow bounded by steeply inclined both margins, elevated marginal rims and sharp pointed both longitudinal furrow ends. This trace fossil is herein described as Lockeia gigantus ichnosp. nov. Plicatounio, a freshwater bivalve which does not occur occurs occasionally within Lockeia gigantus is regarded as the most-likely producer of this resting trace fossil. This new trace fossil represents the largest Lockeia ever known and the first record of Lockeia from the Cretaceous non-marine deposits in the world. This fossil also represents an unusual example of resting trace fossil (Lockeia) associated with a possible producer (bivalve Plicatounio) lived in community in the shallow lacustrine environment.

The Effects of Volume Ratio and Shape on the Formation of Adiabatic Shear Band in WHA (텅스텐 중합금의 부피분율, 입자형상에 따른 단열전단밴드 형성 연구)

  • 이승우;송흥섭;문갑태
    • Transactions of Materials Processing
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    • v.11 no.8
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    • pp.682-690
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    • 2002
  • The formation of adiabatic shearband in tungsten heavy alloys(WHA) was studied in this investigation. Five prismatic specimens were loaded by high velocity impacts and treated as plane strain problems. To find out the effect of particle's volume ratio, specimens containing 81%, 93% and 97% volume percents of tungsten particles were used. Also the effects of particle's geometry and size on the formation of shearband were studied for 81% volume percent alloys by small size particle model, large size particle model and undulated particle models, and the results were discussed.be used to diagnose the causes of necking and fracture in industrial practice and to investigate whether these defects were caused by material property variation, changes in lubrication, or incorrect press settings. In non-axisymmetric deep drawing, three modes of forming regimes are found: draw, stretch, plane strain. The stretch mode for non-axisymmetric deep drawing could be defined when the major and minor strains are positive. The draw mode could be defined when the major strain is positive and minor strain is negative, and plane strain mode could be defined when the major strain is positive and minor strain is zero. Through experiments the draw mode was shown on the wall and flange are one of a drawn cup, while the plane strain and the stretch mode were on the punch head and the punch corner area respectively, We observed that the punch load of elliptical deep drawing was decreased according to increase of die corner radius and the thickness deformation of minor side was more large than major side.