• Journal of Environmental Impact Assessment
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• v.22 no.2
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• pp.125-134
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• 2013

Plant virus coat (CP) gene-mediated protection is one of the best known approaches to protect against virus resistant transgenic plants. Transgenic N. benthamiana plants containing the CP gene of Zucchini green mottle mosaic virus (ZGMMV) were used for the environmental risk assessment of the living modified (LM) plants with plant virus resistance. The most optimal co-infection method of both ZGMMV and CMV (Cucumber mosaic virus) on Non-LM and CP-expressing LM tobacco plants was established and co-infection of CMV and ZGMMV was confirmed by polymerase chain reaction (PCR). To address the effects of LM tobacco plants on the mutation of the virus, in-vitro transcripts of CP and Replicase (Rep) derived from CMV and/or ZGMMV were inoculated onto Non-LM or LM tobacco plants. Mutation frequency of CP and Rep from CMV and ZGMMV was examined through six serial passages in Non-LM and LM tobacco plants. Little actual frequency of mutation was estimated, probably due to the limited number of transgenic plants tested in this study. However, it does not suggest environmental safety of these CP-mediated LM plants. Further study at a larger scale is needed to evaluate the environmental risk associated with the CP-expressing LM plants.

• Journal of the Korean Society of Safety
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• v.33 no.5
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• pp.120-126
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• 2018

The traffic accident types are largely classified into vehicle to vehicle accident, vehicle-to-person accident and single-vehicle. Especially, the single-vehicle accident types are severe when the vehicle crashed into road facilities such as bridge, piers, utility poles. The severity of single-vehicle accidents are ten times higher than that of all other accidents types. It is needed to consider to reduce accident severity. This study was conducted to develop crash worthy safety design facility to ensure the vehicle occupant safety. The simulation and the crash tests were conducted for assessment of the safety performance to check the criteria of CC2(Crash Cushion 2) level. THIV(Theoretical Head Impact Velocity) and PHD(Post-impact Head Deceleration) were used to assess occupant impact severity for crashes. The non-redirection collision test conditions for 900 kg and 1,300 kg-head on crash tests, 900 kg-1/4 offset crash tests, 1,300 kg-head on crash test with $15^{\circ}$angle were conducted. The simulation and experiment test result showed that THIV values were below 44 km/h criterion, PHD values were below the 20G. The development non-redirective crash cushion is expected to be used for the fixed object such as bridge piers for assuring occupant safety.

• 한국연소학회:학술대회논문집
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• 2014.11a
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• pp.285-288
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• 2014

Experiments have been performed for the burners used in the non-oxidizing direct fired furnaces for the cold rolled plate to investigate the effect of fuel/air mixing patterns of the burner nozzle on flame shape, temperature and combustion gas concentration. CFD simulation has also been performed to investigate the mixing state of air-fuel for a nozzle mixing burner and a partially pre-mixing burner. A partially pre-mixing burner showed that flame temperature increased up to $26^{\circ}C$ on average compared than that of the nozzle mixing. It also showed that the mixing distance is important at the partially pre-mixing burner. Test results for a partially pre-mixing burner showed that the residual oxygen concentration and the volume ratio of $CO/CO_2$ of the flame were applicable to be used in field furnaces.

• Nutritional Sciences
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• v.2 no.2
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• pp.93-101
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• 1999

The aim of this study was to investigate influence of body build on body composition, energy metabolic state and insulin concentration of blood. 29 male athletes and 36 male non-athletic students were recruited for the study. Anthropometry including chest depth and breadth, fat mass, fat fee mass, tricep skinfold thickness were measured. fasting glucose, lactate, triglyceride, fee fatty acid, and insulin concentration in serum were measured . Body build was assessed using metric index, which calculated by regression equations of Mohr and Greil. The athletic and non-athletic students were allocated to 3 body build, that is leptomorph, mesomorph, and pyknomorph. Resting metabolic rate was calculated. Respiratory quotient was determined through ratio of measured VO$_2$, and V$CO_2$. Most non-athletes have a leptomorphic body build, in contrast to athletes mesomorphic type. The body build type influenced body composition differently between non-athletic group and athletic group. Weight, body mass index, body fat mass and fat mass proportion (%), and fat-free mass increased from leptomorph to pyknormorph in non-athletic group. Pyknormorphic athletes have a significant higher body mass index, fat mass, fat free mass than other body build type. Serum glucose, triglyceride, lactate, insulin showed significant differences only in non-athletic group between leptomorph and mesomorph. RMR increased significantly from leptomorph to mesomorph in non-athletes. There was no significant difference of RQ among 3 body build types in both athletes and non-athletes. This study gives a coherent data on body build and body composition for athletes and non-athletes students. The influence of body builds on energy metabolic status of serum was different between athletes and non-athletes.

• Bulletin of the Korean Chemical Society
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• v.30 no.2
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• pp.389-396
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• 2009

MFI structural zeolite (ZSM-5 or Sililcalite) was treated with HF solution to introduce mesoporous channels in the microporous crystals. Inner mesopore size could be controlled from 2.5 to 3.5 nm by changing the concentration of HF solution. The pore structure of HF-treated MFI zeolite was studied by instrumental analysis. The active Co (III) salen complex monomers were successfully anchored non-covalently on the surfaces of mesoporous MFI-type zeolite. These heterogeneous catalysts could be applied in asymmetric ring opening of terminal epoxides by phenol derivatives. It showed very high enantioselectivity and yield up to 95% in the catalytic synthesis of optically active $\alpha$-aryloxy alcohol compounds.

• Journal of Embryo Transfer
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• v.10 no.2
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• pp.115-120
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• 1995

This study was carried out to investigate On in vitro fertilization, survival rate and developmental rate of rapidly frozen bovine immature oocytes. Immature oocytes cultured for 1, 12, 24, 48 hours in 20% FCS + TCM-199 medium and thereafter rapidly freezing-thawed oocytes inseminated with capacitated sperm. The immature oocytes following dehydration by 1.5M DMSO + 2.0M glycerol + 0.25M sucrose + TCM 199 media + 20% FGS were directly plunged into liquid nitrogen and thawes in 3$0^{\circ}C$ water. Rapid freezing embryos co-cultured in 20% FCS + TCM-199 media containing hormones(21U/mL PMSG, 21U /mL hGG and 1 $\mu$g /mL 17$\beta$-estradiol) and cumulus cells(1 x 105-6 cells). Survival rate was defined as development rate on in vitro culture or FDA-test. The results are summarized as follows ; 1. The in vitro maturation and fertilization rate of immature bovine oocytes on in vitro maturation period(1, 12, 24, 48 hrs) before rapid freezing4hawed were 57.1%, 45.7%, 37.1%, 25.7% and 40.0%, 31.4%, 20.0%, 11.4%, respectively. 2. The survival rate of immature bovine oocytes on in vitro maturation period(1, 12, 24, 48 hrs) before rapid freezing-thawed were 33.3%, 26.7%, 20.0%, and 10.0%, respectively. The survival rate of rapid freezing4hawed immature oocytes was significantly lower than that of non-freezing oocytes. 3. The survival rate of rapid freezing4hawed excellent and good bovine embryos co-cultured in 20% FCS + TCM-199 media containing hormones(PMSG, hCG, 17$\beta$-estradiol) and cumulus cells 4 to 5 hrs and 20 to 24 hrs were 35.0%, 15.0% and 25.0%, 15.0% and 40.0%, 20.0% and 30.0%, 15.0%, respectively. The survival rate of embryos co-cultured in TCM-199 media containing hormones and cumulus cells was significantly higher than that of non co-culture.

• Clinical and Experimental Reproductive Medicine
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• v.35 no.1
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• pp.49-60
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• 2008

Objective: This study was carried out to investigate the effects of 3-dimensional co-culture of human endometrial cells decidualized with progesterone and TGF-${\beta}1$ on the development of 2-cell mouse embryos. Methods: Stromal and epithelial cells isolated from human endometrial tissue were immunostained for cytokeratin and vimentin. Expression of TGF-${\beta}1$, its receptor-1, -2, integrin-${\beta}3$ and prolactin in mono or co-culture according to three different hormone conditions was investigated by RT-PCR. Differential staining was used to investigate the number of ICM and trophectoderm of hatched mouse blastocysts in different three conditions. Results: The immunohistochemical study was positive for cytokeratin or vimentin and confirmed that epithelial and stromal cells were isolated from endometrial tissue successfully. In co-culture, TGF-${\beta}1$, its receptor-1, integrin-${\beta}3$ and prolactin except TGF-${\beta}1$-r2 were expressed in progesterone dominant condition. The hatching and attaching rate were higher in the co-culture with decidualized cells (p<0.05). However, we observed that lots of the incomplete hatched blactocysts attached on non-decidualized cells. The ICM number of hatched mouse blastocysts was higher in co-culture with decidualized and non decidualized cells than media only culture (p<0.05). The trophectoderm number of hatched blastocyst was higher in the co-culture with decidualized cells than non-decidualized cells or media only culture (p<0.05). Conclusion: The administration of progesterone, estrogen and TGF-$\beta$ could induce decidualization of stromal and epithelial cells isolated from human endometrial tissue using 3-dimensional co-culture, and the decidualization of human endometrial cells could increase the hatching and attaching rate of 2-cell mouse embryos.

• Journal of Ocean Engineering and Technology
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• v.1 no.2
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• pp.39-51
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• 1987

석유시추 보호관의 비선형 운동을 시뮬레이트하는 유효한 해법이 non-uniform grid 유한차분법과 implicit time 적분법에 근거하여 제시되었다. 극한 상태에 있는 지지 플랫폼의 상승 가속도에 의해 생기는 보호관의 동적 좌굴형 반응에 관하여 상세히 연구되었고, 이 반응에 미치는 중요 변수가 규명되었다. 운동의 현저한 감소와 이에 따른 응력들이 컴플라이언트 행오프(hang-off)를 적용시킴으로써 얻어졌다.

• Proceedings of the Korean Magnestics Society Conference
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• 2002.12a
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• pp.190-191
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• 2002

Granular feromagnets는 non-magnetic maxtrix 안에 nanometer-sized의 ferromagnetic grain들 구성된다. Co-Cu,Co-Ag, Fe-Ag, NiFe-Ag $^1$을 포함하는 이미 알려진 다른 Granular 금속 합금들의 giant magnetoresistance 에 관계하여 Granular feromagnets 에 대해 조사하였다. Bulk상태의 NiFe와 Mn 혼화되기 쉽다.$^2$ 그리고 Mn은 열처리된 다층박막의 NiFe의 lattice에 쉽게 수용되어진다.$^3$ 이번 실험에서는 metastable한 fcc solid solution NiFe-Mn 박막이 열처리 하에서 NiFe과 Mn으로 분리되었다. (중략)

• Proceedings of the Korean Vacuum Society Conference
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• 2016.02a
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• pp.378.2-378.2
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• 2016

Biosensors currently suffer from severe non-specific adsorption of proteins, which causes false positive errors in detection through overestimation of the affinity value. Overcoming this technical issue motivates our research. Polyethylene glycol (PEG) is well known for its ability to reduce the adsorption of biomolecules; hence, it is widely used in various areas of medicine and other biological fields. Likewise, amine functionalized surfaces are widely used for biochemical analysis, drug delivery, medical diagnostics and high throughput screening such as biochips. As a result, many coating techniques have been introduced, one of which is plasma polymerization - a powerful coating method due to its uniformity, homogeneity, mechanical and chemical stability, and excellent adhesion to any substrate. In our previous works, we successfully fabricated plasmapolymerized PEG (PP-PEG) films [1] and amine functionalized films [2] using the plasma enhanced chemical vapor deposition (PECVD) technique. In this research, an amine functionalized PP-PEG film was fabricated by using the plasma co-polymerization technique with PEG 200 and ethylenediamine (EDA) as co-precursors. A biocompatible amine functionalized film was surface characterized by X-ray photoelectron spectroscopy (XPS) and Fourier-transform infrared spectroscopy (FT-IR). The density of the surface amine functional groups was carried out by quantitative analysis using UV-visible spectroscopy. We found through surface plasmon resonance (SPR) analysis that non-specific protein adsorption was drastically reduced on amine functionalized PP-PEG films. Our functionalized PP-PEG films show considerable potential for biotechnological applications such as biosensors.