• Journal of Microbiology and Biotechnology
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• 제6권6호
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• pp.369-374
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• 1996

The ${\beta}$-lactamase inhibitory protein (BLIP) produced by Streptomyces exfoliatus SMF19 was purified(33 kDa) and the N-terminal amino acid sequence was determined as NH2-ATSVVAWGGNND. Genomic DNA library of S. exfoliatus SMF19 was constructed in pWE15 and recombinants harbouring the corresponding gene were selected by colony hybridization to the mixture of 36-mer oligonucleotide designed from the N-terminal amino acid sequence. The corresponding gene (bliX) was isolated on a 4-kb ApaI fragment of S. exfoliatus SMF19 chromosomal DNA and then sequenced. The bliX consisting of 1, 119bp encoded a mature protein with a deduced amino acid sequence of 342 residues and also encoded a 40-amino-acid signal sequence. No significant sequence similarity to bliX was found by pairwise comparison using various protein and nucleotide sequences.

• 전력전자학회논문지
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• 제20권4호
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• pp.321-329
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• 2015

The static synchronous compensator (STATCOM) of cascaded H-bridge configuration accompanying multiple separate DC sides is inherently subject to the problem of uneven DC voltages. These DC voltages in one leg can be controlled by adjusting the AC-side output voltage of each cell inverter, which is proportional to the active power. However, when the phase current is extremely small, large AC-side voltage is required to generate the active power to balance the cell voltages. In this study, an alternative zero-sequence current injection method is proposed, which facilitates effective cell balancing controllers at no load, and has no effect on the power grid because the injected zero sequence current only flows within the STATCOM delta circuit. The performance of the proposed method is verified through simulation and experiments.

• 스마트미디어저널
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• 제7권4호
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• pp.17-23
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• 2018

자동 띄어쓰기 특성을 효과적으로 처리할 수 있는 LSTM(Long Short-Term Memory Neural Networks) 기반의 RNN 모델을 제시하고 적용한 결과를 분석하였다. 문장이 길거나 일부 노이즈가 포함된 경우에 신경망 학습이 쉽지 않은 문제를 해결하기 위하여 입력 데이터 형식과 디코딩 데이터 형식을 정의하고, 신경망 학습에서 드롭아웃, 양방향 다층 LSTM 셀, 계층 정규화 기법, 주목 기법(attention mechanism)을 적용하여 성능을 향상시키는 방법을 제안하였다. 학습 데이터로는 세종 말뭉치 자료를 사용하였으며, 학습 데이터가 부분적으로 불완전한 띄어쓰기가 포함되어 있었음에도 불구하고, 대량의 학습 데이터를 통해 한글 띄어쓰기에 대한 패턴이 의미 있게 학습되었다. 이것은 신경망에서 드롭아웃 기법을 통해 학습 모델의 오버피팅이 되지 않도록 함으로써 노이즈에 강한 모델을 만들었기 때문이다. 실험결과로 LSTM sequence-to-sequence 모델이 재현율과 정확도를 함께 고려한 평가 점수인 F1 값이 0.94로 규칙 기반 방식과 딥러닝 GRU-CRF보다 더 높은 성능을 보였다.

• 식물병연구
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• 제29권3호
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• pp.286-294
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• 2023

Biological and molecular characterization of a Korean isolate of Orthotospovirus chrysanthinecrocaulis (formerly known as chrysanthemum stem necrosis virus, CSNV) isolated from Chrysanthemum morifolium was determined using host range and sequence analysis in this study. Twenty-three species of indicator plants inoculated mechanically CSNV-Kr was investigated for determination of host range. CSNV-Kr induced various local and systemic symptoms in the inoculated plant species. CSNV-Kr could not infect three plant species and induced symptomless in systemic leaves in Nicotiana tabacum cultivars, though the plant samples reacted positively with the antiserum to CSNV by double-antibody sandwich-enzyme-linked immunosorbent assay. The complete genome sequence of CSNV-Kr was determined. The L RNA of CSNV-Kr consists of 8,959 nucleotides (nt) and encodes a putative RNA-dependent RNA polymerase. The M RNA of CSNV-Kr consists of 4,835 nt and encodes the movement protein (NSm) and the glycoprotein precursor (Gn/Gc protein). The S RNA of CNSV-Kr consists of 2,836 nt and encodes NSs protein and N protein. The Gn/Gc and N sequence of CSNV-Kr were compared with those of previously published CSNV isolates originating from different countries at nucleotide and amino acid levels. The Gn/GC sequence of CSNV-Kr shared 98.8-99.5% identity with CSNV isolated from other countries and the N sequence of CSNV-Kr shared 98.8-99.6% identity. No particular region of variability could be found in either grouping of viruses. All of the CSNV isolates did not show any relationship according to geographical origins and isolation hosts, suggesting no distinct segregation of the CSNV isolates.

• 생명과학회지
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• 제14권6호
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• pp.963-966
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• 2004

능이버섯의 16S ribosomal DNA일부분, IT1, 5.85 ribosomal DNA, ITS2의 전부분, 28S ribosomal DNA 일부분이 포함된 ITS영역의 염기서열을 분석하였다. 이 부분은 716개의 염기쌍으로 구성되었다. 이를 Sarcadon속에 속하는 종들과 비교분석한 결과 같은 능이버섯의 ITS에 관한 다른 분석결과 염기치환 및 결실을 근거로 하였을 경우 $1.8\%$의 차이를 나타내었다. 또한 S. imbricatus와는 $1.8\%$, S. sequamous와는 $10\%$차이를 나타내었다. 이는 능이버섯이 숙주, 서식지 환경 등의 특수성 때문에 자연상태에서 유전자교류가 일어나지 않기 때문인 것으로 생각된다.

• 한국산학기술학회논문지
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• 제11권1호
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• pp.107-112
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• 2010

PLC를 사용한 제어시스템에서 SFC 언어를 사용하면 전체 공정의 흐름을 이해하기 쉽고 유지보수가 용이하다. SFC 언어는 단일 시퀀스, 선택 시퀀스, 병렬 시퀀스로 나누어지고, 여러 조건에 맞게 처리하는 선택시퀀스로 프로그램 할 때 선택분기 스텝에 에러가 발생하면 전체 공정이 정지하게 된다. 이 에러스텝이 전체 공정에 크게 영향을 안주는 공정이라면 전체 공정을 정지시키지 않고 에러를 처리하면 손실은 줄어들게 된다. 본 논문에서는 선택시퀀스에서 리모트 콘트롤을 사용하여 선택시퀀스를 효율적으로 제어하는 방법을 제시하고 적용 예를 통해 그의 타당성을 확인하였다.

• 한국통신학회논문지
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• 제25권8B호
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• pp.1333-1340
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• 2000

본 논문에서는 고속의 초기동기가 가능한 Kronecker 부호를 DS/CDMA 시스템의 확산부호로 하여 패킷 무선 데이터 통신에서의 사용을 위한 타당성을 보인다 먼저 Kronecker 부호의 생성방법 상관특성을 보여 대체적인 부호의 특성을 밝히고 부분 상관기를 이용할 수 있음을 보인다 더 나아가 PN 부호와 같은 평균 초기동기획득 시간을 가질 때의 Kronecker 부호의 오경보 확률과 실패 확률을 구하여 Kronecker 부호가 PN 부호보다 더 높은 동기 확률을 가짐을 보임으로써 패킷 통신에서 Kronecker 부호를 사용할 수 있는 가능성을 확인한다.

• 치과방사선
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• 제20권2호
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• pp.187-198
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• 1990

Examinations of the temporomandibular joints were performed on a 1.5 Tesla magnetic resonance (MR) system. An MR surface receiver coil 3 inch in diameter was placed on plastic frame, the patient's head being placed in the frame so that the coil was pressed against the temporal region. In taking advantage of the magnetic resonance imaging that has been studied briskly till now, author obtained the images of parasagittal and paracoronal planes about the temporomandibular joint by using MPGR (Multi-Planar Gradient Recalled), GRASS (Gradient Recalled Acquisition in the Steady State), and CSMEMP (Contiguous Slice Multiple Echo, Multi-Planar), that differ from the Spin Echo pulse sequence which the previous authors used. Five subjects with no symptoms of temporomandibular joint pain and dysfunction were studied. The plane images obtained by these methods were compared with those by Spin Echo pulse sequence. The results were as follows: 1. The optimal repetition times (TR) and echo times (TE) for T.M.J. image were; a. 400 msec and 18 msec in PMGR pulse sequence. b. 40 msec and 12 msec in GRASS pulse sequence. c. 700 msec and 30 msec in CSMEMP pulse sequence. d. 500 msec and 20 msec in Spin Echo pulse sequence. 2. When the MPGR pulse sequence was using, T2-weighted image was obtained in very short time. On the image of the paracoronal plane by GRASS pulse sequence, meniscus showed the moderate signal intensity, and the meniscus and its anteromedial, posterolateral attachments were observed definitely with gray color. 4. The signal intensity of Spin Echo pulse sequence was equal to that of CSMEMP pulse sequence, but the image by CSMEMP pulse sequence showed relatively lower level in its resolution.

• 품질경영학회지
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• 제17권1호
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• pp.69-81
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• 1989

This study is concerned with a job sequencing method using the concept of sampling technique in the case of Job Shop. This is the follow study of Kang and Ro (1988) which examined the possibility of application of sampling technique to determine the Job Sequence in the case of Flow Shop. Not only it is very difficult, but also it takes too much time to develop the appropriate job schedules that satisfy the complex work conditions. The most job sequencing algorithms have been developed to determine the best or good solution under the special conditions or assumptions. The application areas of these algorithms are also very narrow, so it is very hard to find the appropriate algorithm which satisfy the complex work conditions. In this case it is very desirable to develop a simple job sequencing method which can select the optimal job sequence or near optimal job sequence with a little effort. This study is to examine the effect of sampling job sequencing which can select the good job of 0.01%~5% upper good group. The result shows that there is the sets of 0.05%~23% job sequence group which has the same amount of performance measure with the optimal job sequence in the case of experiment of N/M/G/$F_{max}$. This indicates that the sampling job sequencing method is a useful job sequencing method to find the optimal or good job sequence with consuming a small amount of time. The results of ANOVA show that the only one factor, number of machines is the significant factor for determining the job sequence at ${\alpha}=0.01$. It takes about 10 minutes to compare the number of 10,000 samples of job sequence by personal computer and it is proved that the selection rate of the same job sequence with optimal job sequence is 23.0%, 3.9% and 0.065% in the case of 2 machines, 3 machines and 4 machines, respectively. The area of application can readily be extended to the other work condition.

• Communications for Statistical Applications and Methods
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• 제8권3호
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• pp.615-623
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• 2001

A functional central limit theorem is obtained for a stationary multivariate linear process of the form (no abstract. see full-text) where{ $Z_{t}$} is a sequence of strictly stationary m-dimensional negatively associated random vectors with E $Z_{t}$=O and E∥ $Z_{t}$∥$^2$<$\infty$ and { $A_{u}$} is a sequence of coefficient matrices with (no abstract. see full-text) and (no abstract. see full-text).text).).