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Analysis of ITS DNA Sequences of the Viola albida Complex (태백제비꽃군 ITS DNA 염기서열 분석)

  • Whang, Sung-Soo
    • Korean Journal of Plant Resources
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    • v.19 no.5
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    • pp.628-633
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    • 2006

  • ITS DNA sequences from five individuals, representative of five groups designated according to the degree of leaf teeth and lobes from simple to palmate compound leaf in the Viola albida complex, established and further analysed in order to solve the taxonomic difficulty. A total 702 bp was sequenced at the 5.8S ribosomal DNA and internal transcribed spacer 1 and 2. The 5.8S coding region is 163 bp, and has no sequence variations. The ITS1 and ITS2 noncoding regions have a little bit sequence variations, and those were further analysed by the methods of the analysis of variance (ANOVA), the analysis of sequence divergence and the phylogenetic analysis. The result of ANOVA showed no significant differences among individuals investigated. The analysis of sequence divergence with Kimura 2-parameter distance revealed that in-groups showed much less than 0.05 in absolute value among individuals, while two out groups more than 0.05, V. grypoceras and V. orientalis. This result appeared that the sequence divergence among in-groups was not yet occurred in the species level but situated at somewhere below the species level. In the phylogenetic analysis, two outgroups formed the basal clades in order. Five individuals in-groups formed a clade. The clade was, however, not very robust as around 50% in bootstrap value, suggesting that this result was not meaningful in the phylogenetic point of views.

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A Study of Over Voltage Ground Relay Operation Status at Opening of No-load Charged Cable (무부하 충전케이블 개방시 잔류전압에의한 과전압계전기 동작현상 연구)

  • Kim, Yeong-Han;Choi, Jong-Hyuck;Yoon, Ki-Seob
    • Proceedings of the KIEE Conference
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    • 2000.07a
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    • pp.185-187
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    • 2000

  • Fault current is flowed into 154/23kV M. Tr when line-to-ground fault occurs in power system. NGR(Neutral Grounded Reactor) is set up in order to prevent M.Tr fault by limiting magnitude of fault currents. Here, disconnection of NGR causes voltage increase by L-C resonance and line-to-ground fault in an unearthed system results in voltage increase at healthy phases. So Over Voltage Ground Relay(OVGR) is used for tripping M.Tr. Also, buses at second phases of M.Trs are all connected with section circuit breakers closed for the purpose of parallel operation and load shedding. In case of speciality buses are comprised of power cable in part for GIS connection. When no-load charged cable or bus is open by a section CB, unbalanced voltage charged on the bus is induced. Also discrepant opening time for circuit breakers on different phases gives rise to unbalanced zero sequence voltage. It was observed that this zero sequence voltage detected in the 22.9kV P.T (Potential Transformer for bus) mal-operated 59GT and tripped M.Tr. The zero sequence voltage of which vanishing time is longer than relay operating time came out by EMTDC simulation. Also, it was shown that the voltage waves of actual test are similar to those of simulation. On the basis of above results, R-C circuit complement on the relay without any effect on a power system made operating time of the relay longer than vanishing time of distorted waves. Consequently, operating time of the relay was delayed and magnitude of distorted waves was decreased by increasing time constant of the relay.

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Intronic Sequences for the Discrimination of Silkworm Strains in Silkworm Powder

  • Kim, Kee-Young;Lee, Eun-Mee;Hong, Mee-Yeon;Kang, Pil-Don;Ryu, Kang-Sun;Kim, Ik-Soo
    • International Journal of Industrial Entomology and Biomaterials
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    • v.15 no.2
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    • pp.93-100
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    • 2007

  • After a serial study on the therapeutic efficacy of the powdery silkworm for diabetics was positively resulted in, many powdery silkworm products were on the market in Korea. Up to now, however, no causal method is available to discriminate the strain of silkworms that is a major ingredient for manufacturing powdery silkworm, even though the quality of the powdery silkworm differs greatly by source and origin of strains. We previously were successful in identifying 25 silkworm strains kept in Korea using nine intronic sequences. In this study, we tested the utility of the nine intronic sequences to distinguish the most widely reared silkworm strains originated from Korea and China. Two intron regions, PTTH Intron3 and PTTH Intron3, showed a substantial sequence divergence (mean sequence divergence of 3.13% in PTTH Intron3 and 4.99% in PTTH Intron3). These two intronic sequences provided no identical sequences among the seven strains tested. Thus, these sequences each along can be used to discriminate the seven strains tested in this study. Furthermore, other intron regions, except for VDP Intron4 allowed us to discriminate $2{\sim}4$ strains by strain-specific unique insertion/deletion or substitution.

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