• ALGAE
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• 제26권3호
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• pp.229-235
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• 2011

Spliced leader (SL) trans-splicing is a mRNA processing mechanism in dinoflagellate nuclear genes. Although studies have identified a short, conserved dinoflagellate SL (dinoSL) sequence (22-nt) in their nuclear-encoded transcripts, whether the majority of nuclear-coded transcripts in dinoflagellates have the dinoSL sequence remains doubtful. In this study, we investigated dinoSL-containing gene transcripts using 454 pyrosequencing data (Cochlodinium polykrikoides, 93 K sequence reads, 31 Mb; Prorocentrum minimum, 773 K sequence reads, 291 Mb). After making comparisons and performing local BLAST searches, we identified dinoSL for one C. polykrikoides gene transcript and eight P. minimum gene transcripts. This showed transcripts containing the dinoSL sequence were markedly fewer in number than the total expressed sequence tag (EST) transcripts. In addition, we found no direct evidence to prove that most dinoflagellate nuclear-coded transcripts have this dinoSL sequence.

• 한국군사과학기술학회지
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• 제11권5호
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• pp.23-33
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• 2008

In this paper, we verify the performance of LDPC coded OFDM/DS system by Monte-Carlo simulation of BER on Eb/No. The simulation results show that LDPC coded OFDM/DS has a strong anti-jamming characteristic over pulse-noise jammer and partial-band noise jammer. The performance of LDPC coded OFDM/DS system is evaluated on both faded waveforms and non-faded waveforms. For non-faded waveforms, high coding gain is attained due to LDPC, even when waveforms have short PN sequence and JSR is only 5dB. Especially, the increase in the repeated number of LDPC decoding enhances coding gain. However, faded waveforms cannot achieve sufficient average effect when PN sequence is short. High coding gain of faded waveforms can be achieved by extending length of PN sequence. In addition, we compare LDPC coded OFDM/DS system with Convolutional coded OFDM/DS system. The simulation results illustrate that when LDPC coded OFDM/DS system with short PN sequence has sufficient average effects, the system shows lower BER than Convolutional coded OFDM/DS system with long PN sequence.

• 한국통신학회논문지
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• 제33권11C호
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• pp.874-879
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• 2008

[1]에서 LCZ 수열군의 2배 확장을 제안하였다. 본 논문에서는 [1]에서의 2배 확장방법을 일반화하는 새로운 확장방법을 제안한다. 이 생성방법을 사용하면 인수가 (N,M,L,${\epsilon}$)인 q진 LCZ 수열군은 인수가 (pN,pM,p[(L+1)/p]-1,p${\epsilon}$)인 q진 LCZ 수열군이 된다. 이 때, p는 소수이고 p는 q의 약수다. 특히 L${\equiv}$p-1modp일 때, 확장된 q진 LCZ 수열군의 인수는 (pN,pM,L,p${\epsilon}$)이 된다.

• Journal of Microbiology and Biotechnology
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• 제25권8호
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• pp.1234-1240
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• 2015

Heat shock RNA 1 (HSR1) is described as a "eukaryotic heat-sensing noncoding RNA" that regulates heat shock response in human and other eukaryotic cells. Highly conserved HSR1 sequences have been identified from humans, hamsters, Drosophila, Caenorhabditis elegans, and Arabidopsis. In a previous study, however, it was suggested that HSR1 had originated from a bacterial genome. HSR1 showed no detectible nucleotide sequence similarity to any eukaryotic sequences but harbored a protein coding region that showed amino-acid sequence similarity to bacterial voltage-gated chloride channel proteins. The bacterial origin of HSR1 was not convincible because the nucleotide sequence similarity was marginal. In this study, we have found that a genomic contig sequence of Comamonas testosteroni strain JL14 contained a sequence virtually identical to that of HSR1, decisively confirming the bacterial origin of HSR1. Thus, HSR1 is an exogenous RNA, which can ectopically trigger heat shock response in eukaryotes. Therefore, it is no longer appropriate to cite HSR1 as a "eukaryotic functional noncoding RNA."

• 한국미생물·생명공학회지
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• 제31권2호
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• pp.111-116
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• 2003

Xylan 분해 균주인 Bacillus stearothermophilus No. 236 분리균의 $\beta$-xylosidase 생산 유전자(xylA)의 염기 서열 및 transcription start site를 결정한 이전 연구 결과에 의하면 xylA 유전자는 매우 특이하게 UUG codon에서 translation이 시작되며 initiation codon 15dp 윗쪽에는 promoter로 추정되는 염기 서열을 가지고 있는 것으로 분석되었다. 이와 같은 xylA 유전자 promoter region의 구조는 E. coli에 클로닝된 xalA 유전자를 이용한 실험 결과로도 확인되었다. xalA promoter의 -10 element는 CATAAT로서 6개의 염기 중 5개가 그리고 -35 element의 경우는 TTGTTA로서 6개의 염기 중 4개가 consensus sequence와 일치되었으나 두 hexamer 사이의 거리가 최적 거리에서 크게 벗어난 12 bp인 것으로 분석되었다. 본 연구에서는 $\beta$-xylosidase의 대량 생산을 위한 연구의 일환으로 xalA promoter sequence의 체계적 구조 변화에 의한 promoter strength에 미치는 효과를 E. coli와 B. subtilis두 숙주 세포에서 조사 분석해 본 결과, 첫째로 두 promoter elements사이의 거리를 최적거리인 17 bp로 바꾸었을 때 xalA의 발현율은 E. coli에서는 1.6배, B. subtilis에서는 2.5배 정도 증가함을 보여주었다. 그리고 -35 element는 consensus sequence와 같이 5'쪽에서 네번째 위치에 있는 T$\longrightarrow$A로 변이 시켰을 때 E. coli경우 2.3배, 특히 B. subtilis에서는 35배나 되는 가장 높은 promoter 활성의 증가를 보였다. 그러나 -10 sequence의 경우 consensus sequence와 같이 5' 쪽에서 첫번째 위치에 있는 C$\longrightarrow$T로 transition시켰을 때 예상외로 오히려 발현율이 5~15배까지 낮아지는 특이한 결과를 얻었다. 따라서 본 연구 결과 xalA promoter의 경우 -10 sequence인 CATAAT의 C와 -35 element의 두 염기가 promoter활성에 있어 가장 중요한 염기임을 알 수 있었다.

• 제어로봇시스템학회:학술대회논문집
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• 제어로봇시스템학회 1992년도 한국자동제어학술회의논문집(국제학술편); KOEX, Seoul; 19-21 Oct. 1992
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• pp.463-467
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• 1992

This paper describes the automatic generation of sequence control programs for DCS(Distributed Control System), PLC(Programable Logic Controller) and so on. Since there is no same manufacturing process, it is difficult to standardize sequence programs. We propose the automatic sequence control program generator which is CAD software using knowledge engineering technique.

• Journal of Communications and Networks
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• 제14권3호
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• pp.230-236
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• 2012

Based on the known binary sequence sets and Gray mapping, a new method for constructing quaternary sequence sets is presented and the resulting sequence sets' properties are investigated. As three direct applications of the proposed method, when we choose the binary aperiodic, periodic, and Z-complementary sequence sets as the known binary sequence sets, the resultant quaternary sequence sets are the quaternary aperiodic, periodic, and Z-complementary sequence sets, respectively. In comparison with themethod proposed by Jang et al., the new method can cope with either both the aperiodic and periodic cases or both even and odd lengths of sub-sequences, whereas the former is only fit for the periodic case with even length of sub-sequences. As a consequence, by both our and Jang et al.'s methods, an arbitrary binary aperiodic, periodic, or Z-complementary sequence set can be transformed into a quaternary one no matter its length of sub-sequences is odd or even. Finally, a table on the existing quaternary periodic complementary sequence sets is given as well.

• 한국버섯학회지
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• 제17권4호
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• pp.185-190
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• 2019

리그닌 분해 담자균류로 알려져 있는 느타리버섯균 No.42는 망간퍼옥시데이즈(MnP) 및 락게이즈(Lac)를 생산하였으나 글루코오스-펩톤-이스트-밀기울(GPYW)배지를 이용한 정치배양조건하에서 리그닌퍼옥시데이즈(LiP)활성은 검출되지 않았다. 한편, 동일배지에서 망간퍼옥시데이즈 활성은 11일째 80(3.6) U/flask(ml)의 최대 생산되었다. 망간퍼옥시데이즈 분리정제는 Sepha-ros CL-6B 및 Mono-Q 컬럼순으로 수행하였으며 주요 망간퍼옥시데이즈 isozyme은 단일밴드로 분자량은 36.4KDa이였다. N-말단으로부터 19개의 아미노산 배열은 단백질 자동 분석 장치로 분석한 결과 ATCADGRTTANAACCVLFP를 나타내었다. 느타리버섯균 No.42의 정치배양조건 하에서 세포외로 생산되는 중요한 망간퍼옥시데이즈 isozyme의 N-말단 아미노산 배열은 이전에 보고된 MnP3의 아미노산 배열과 동일하였다.

• 한국군사과학기술학회지
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• 제9권4호
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• pp.32-38
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• 2006

In this paper a sequence estimator of CDMA communication system is suggested. A sequence estimator uses Galois Field operation. A sequence estimator can provide another CDMA pilot signal which is un-modulated spreaded signal. A estimated sequence signal and received signal have no correlation. Tow signals can be summed using MRC(maximal ratio combine) method. The stronger signal can be added as a larger ratio, but the weaker signal can be added as a smaller ratio. We can distinguish strong signal using SNR estimator. Therefore it is possible to receive an additional pilot signal, and to support more reliable communications by using sequence estimator.

• BMB Reports
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• 제37권2호
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• pp.144-147
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• 2004

Molecular amplification and sequencing of genomic DNA that encodes camel polyubiquitin (PUBC1) was performed by a polymerase chain reaction (PCR) using various sets of primers. The amplification generated a number of DNA fragments, which were sequenced and compared with the polyubiquitin coding sequences of various species. One DNA fragment that conformed to 325 bp was found to be 95 and 88% homologous to the sequences of human polyubiquitin B and C, respectively. The DNA translated into 108 amino acids that corresponded to two fused units of ubiquitin with no intervening sequence, which indicates that it is a polyubiquitin and contains at least two units of ubiquitin. Although, variations were found in the nucleotide sequence when compared to those of other species, the amino acid sequence was 100% homologous to the polyubiquitin sequences of humans, mice, and rats. This is the first report of the polyubiquitin DNA coding sequence and its corresponding amino acid sequence from camels, amplified using direct genomic DNA preparations.