• Title/Summary/Keyword: Nitrogen flow ratio

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Viability Assessment of Fresh and Frozen-thawed Dog Spermatozoa by Flow Cytometry (Flow Cytometry에 의한 개 신선정액과 동결정액의 생존성 분석)

  • Hong Y. M.;Kim Y. J.;Yu I.;Ji D. B.;Kim M. S.
    • Reproductive and Developmental Biology
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    • v.28 no.3
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    • pp.167-172
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    • 2004
  • This study was performed to examine the correlations among dog sperm viabilities evaluated by flow cytometry, by microscopic evaluation (ME), by carbo-xifluorescein diacetate and propidium iodide (CFDA/PI) and by hypoosmotic swelling (HOS) test. Semen were collected from 5 dogs ranging in age from 2 to 4 years. Each ejaculate was divided into 3 aliquots and different proportions of freeze-killed cells were added to each aliquot (1:0, 1:1 and 1:3). In the other experiment, semen was extended with Sweden extender containing 5% glycerol and equex STM paste, and frozen using liquid nitrogen vapor. Fresh and frozen-thawed dog sperm viability were assessed by flow cytometry using PI staining method. The accuracy of flow cytometry was evaluated by comparing with other classic assessments, microscopic evaluation, epifluorescence microscopic analysis using CFDA/PI, and HOS test. High correlations of sperm viabilities were found among flow cytometry, epifluorescence evaluation, HOS test (p<0.01) in fresh semen. Especially, sperm viability assessed by HOS test was highly correlated with viability by flow cytometry in all the ratios of live and dead spermatozoa, 1:0, 1:1 and 1:3 (p<0.01). The viability evaluated by ME were significantly correlated with that by flow cytometry in ratios of 1:0 and 1:3 (p<0.05) however, there was no significance in ratio of 1:1. The viability evaluated by C/p were highly correlated with that by flow cytometry in ratio of 1:0 and 1:1 (p<0.01) and significantly correlated in ratio of 1:3 (p<0.05). In frozen-thawed spermatozoa, the viability determined by HOS test was considerably correlated with that by flow cytometry (p<0.01). There was significant correlation between the viabilities by ME and by flow cytometry (p<0.05). But the viability evaluated by CFDA/PI was not correlated with viability by flow cytometry. The result from this study validate the use of flow cytometry as a precise method for assessing the viability of fresh and frozen-thawed dog spermatozoa.

Removal of Organic Matter, Nitrogen and Phosphorus in an UASB Sewage Treatment Process with Recycle of Aerated Bio-Filter Effluent (UASB를 이용한 혐기성 하수처리공정에서 ABF 유출수 반송에 따른 유기물질과 질소 및 인 제거)

  • Tian, Dong-Jie;Byun, Byeong-Su;Lim, Hyun-Suk;Jun, Hang-Bae
    • Journal of Korean Society of Environmental Engineers
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    • v.35 no.5
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    • pp.381-387
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    • 2013
  • Nitrogen and phosphorus removal was investigated in an UASB-ABF (Up-flow anaerobic sludge blanket - aerated bio-filter) anaerobic sewage treatment system. Successful removal of nitrogen and phosphorus with organic matters was possible in the UASB-ABF system from the results of 160 days operation with the influent raw domestic sewage. Removal efficiencies of organic matter (as TCOD) showed 64% in UASB without recycle of the ABF effluent, however, they increased to 92%, 95%, 96% with 120%, 180% and 240% recycle of the ABF effluent, respectively. Increasement of the organic matter removal was not prominent at recycle ratio above 180%. Apparent increase in TN removal occurred with recycle of the ABF effluent. TN removal efficiency was 18% without recycle, but it increased to 82% with 240% recycle of the ABF effluent. And stable nitrification above 95% was possible as a result of efficient removal of organic matter in the UASB with and without recycle of the effluent. Removal of both TP and $PO{_4}^{3-}$-P also increased remarkably with recycle of the effluent. Without recycle of the effluent, that is at strict anaerobic condition in UASB, TP was not removed, however, its removal efficiency increased to 51%, 63%, 71% at recycle ratios of 120%, 180%, 240%, respectively mainly at UASB.

X-ray Scattering Study of Reactive Sputtered Ta-N/Ta/Si(001)Film as a Barrier Metal for Cu Interconnection (구리배선용 베리어메탈로 쓰이는 Ta-N/Ta/Si(001)박막에 관한 X-선 산란연구)

  • Kim, Sang-Soo;Kang, Hyon-Chol;Noh, Do-Young
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2001.05b
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    • pp.79-83
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    • 2001
  • In order to compare the barrier properties of Ta-N/Si(001) with those of Ta-N/Ta/Si(001), we studied structural properties of films grown by RF magnetron sputtering with various $Ar/N_2$ ratios. To evaluate the barrier properties, the samples were annealed in a vacuum chamber. Ex-situ x-ray scattering measurements were done using an in-house x-ray system. With increasing nitrogen ratio in Ta-N/Si(001), the barrier property of Ta-N/Si(001) was enhanced, finally failed at $750^{\circ}C$ due to the crystallization and silicide formation. Compared with Ta-N/Si(001), Ta-N/Ta/Si(001) forms silicides at $650^{\circ}C$. However it does not crystallize even at $750^{\circ}C$. With increasing nitrogen composition in Ta-N/Ta/Si(001), the formation of tantalum silicide was reduced and the surface roughness was improved. To observe the surface morphology of Ta-N/Ta/Si(001) during annealing, we performed an in-situ x-ray scattering experiment using synchrotron radiation of the 5C2 at Pohang Light Source(PLS). Addition of Ta layer between Ta-N and Si(001) improved the surface morphology and reduced the surface degradation at high temperatures. In addition, increasing $N_2/Ar$ flow ratio reduced the formation of tantalum silicide and enhanced the barrier properties.

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Combustion Performance Test of Syngas Gas in a Model Gas Turbine Combustor - Part 1 : Flame Stability (모델 가스터빈 연소기에서 합성가스 연소성능시험 - Part 1 : 화염안정성)

  • Lee, Min Chul;Joo, Seong Pil;Yoon, Jisu;Yoon, Youngbin
    • Journal of the Korean Society for Aeronautical & Space Sciences
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    • v.41 no.8
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    • pp.632-638
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    • 2013
  • This paper describes on the flame stability and combustion instability of coal derived synthetic gas especially for gases of Buggenum IGCC in Netherlands and Taean IGCC in Korea. These combustion characteristics were observed by conducting ambient-pressure elevated-temperature combustion tests in GE7EA model combustor when varying heat input and nitrogen dilution ratio. Flame stability map is plotted according to the flame structure by dividing all regimes into six, and only regime I and II are identified to be stable. Both syngases of Taean and Buggenum with nitrogen integration corresponds to regime II in which syngas burnt stably and flame coupled with outer recirculation flow. Stable regime of Buggenum is larger than that of Taean when considering only $H_2$/CO ratio due to higher content of hydrogen. However, when considering nitrogen dilution, syngas of Taean is burnt more stably than that of Buggenum since more nitrogen in Buggenum has negative effect on the stability of flame.

The Study on Evaluating Performances of Lab Sacle-Advanced $A_{2}O$ with Changing System Using Biofilm Process (생물막 담체를 이용한 실험실 규모 $A_{2}O$공법의 시스템 변형에 따른 고도처리 성능 평가에 관한 연구)

  • Kim, Min-Sik;Kang, Gu-Young
    • Journal of Korean Society of Water and Wastewater
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    • v.26 no.2
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    • pp.209-218
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    • 2012
  • Recently, as reinforced water quality standards for wastewater has been announced, more efficient and more powerful wastewater treatment processes are required rather than the existing activated sludge process. In order to meet this demands, we evaluate Task 1-4 about lab scale $A_{2}O$ process using biofilm media. Task 1, 2, and 3 use 'Module A' which has 4 partitions (Anoxic/Anerobic/Oxic/Oxic). Task 4 uses 'Module B' which has 2 partitions including a denitrification reactor with an Inclined plug flow reactor (IPFR) and a nitrification reactor with biofilm media. The denitrification reactor of Module B is designed to be upward flow using IPFR. The result of evaluating at each Task has shown that attached growth system has better capacity of removal efficiency for organic matter and nitrogen with the exception of phosphorus. Task 4 which has the most outstanding removal efficiency has 90.5% of $BOD_{5}$ removal efficiency, 97.8% of ${NH_4}^{+}-N$ removal efficiency, 65% of T-N removal efficiency and 92% of T-P removal efficiency with additional chemical phosphorus removal system operated at HRT 9hr, Qi:Qir 1:2, and BOD/T-N ratio 2.7.

Band Gap and Defect Sites of Silicon Nitride for Crystalline Silicon Solar Cells (단결정 실리콘 태양전지를 위한 실리콘 질화막의 밴드갭과 결함사이트)

  • Jung, Sung-Wook;Yi, Jun-Sin
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2010.06a
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    • pp.365-365
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    • 2010
  • In this paper, silicon nitride thin films with different silane and ammonia gas ratios were deposited and characterized for the antireflection and passivation layer of high efficiency single crystalline silicon solar cells. As the flow rate of the ammonia gas increased, the refractive index decreased and the band gap increased. Consequently, the transmittance increased due to the higher band gap and the decrease of the defect states which existed for the 1.68 and 1.80 eV in the SiNx films. The reduction in the carrier lifetime of the SiNx films deposited by using a higher $NH_3/SiH_4$ flow ratio was caused by the increase of the interface traps and the defect states in/on the interface between the SiNx and the silicon wafer. The silicon and nitrogen rich films are not suitable for generating both higher carrier lifetimes and transmittance. These results indicate that the band gap and the defect states of the SiNx films should be carefully controlled in order to obtain the maximum efficiency for c-Si solar cells.

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Off-design performance evaluation of multistage axial gas turbines for a closed Brayton cycle of sodium-cooled fast reactor

  • Jae Hyun Choi;Jung Yoon;Sungkun Chung;Namhyeong Kim;HangJin Jo
    • Nuclear Engineering and Technology
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    • v.55 no.7
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    • pp.2697-2711
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    • 2023
  • In this study, the validity of reducing the number of gas turbine stages designed for a nitrogen Brayton cycle coupled to a sodium-cooled fast reactor was assessed. The turbine performance was evaluated through computational fluid dynamics (CFD) simulations under different off-design conditions controlled by a reduced flow rate and reduced rotational speed. Two different multistage gas turbines designed to extract almost the same specific work were selected: two- and three-stage turbines (mid-span stage loading coefficient: 1.23 and 1.0, respectively). Real gas properties were considered in the CFD simulation in accordance with the Peng-Robinson's equation of state. According to the CFD results, the off-design performance of the two-stage turbine is comparable to that of the three-stage turbine. Moreover, compared to the three-stage turbine, the two-stage turbine generates less entropy across the shock wave. The results indicate that under both design and off-design conditions, increasing the stage loading coefficient for a fewer number of turbine stages is effective in terms of performance and size. Furthermore, the Ellipse law can be used to assess off-design performance and increasing exponent of the expansion ratio term better predicts the off-design performance with a few stages (two or three).

Effect of Silicon Oxynitride Matrix on the Optical Properties of Au Nanoparticles Dispersed Composite Film (실리콘 산화질화물 기지상 적용에 따른 Au 나노입자 분산 복합체 박막의 광학적 특성)

  • Cho, Sung-Hun;Lee, Kyeong-Seok
    • Korean Journal of Materials Research
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    • v.19 no.12
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    • pp.637-643
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    • 2009
  • In this study, we analyzed the effect of silicon oxynitride matrix on the optical properties of Au nanoparticles dispersed on composite film and explored the effectiveness of the silicon in fine tuning the refractive index of the composite film for applications in optical waveguide devices. The atomic fraction of nitrogen in $SiO_xN_y$ films was controlled by varying the relative flow ratio of nitrogen gas in reactive sputtering and was evaluated optically using an effective medium theory with Bruggeman geometry consisting of a random mixture between $SiO_2$ and $Si_3N_4$. The Au nanoparticles were embedded in the $SiO_xN_y$ matrix by employing the alternating deposition technique and clearly showed an absorption peak due to the excitation of surface plasmon. With increasing nitrogen atomic fraction in the matrix, the surface plasmon resonance wavelength shifted to a longer wavelength (a red-shift) with an enhanced resonance absorption. These characteristics were interpreted using the Maxwell-Garnett effective medium theory. The formation of a guided mode in a slab waveguide consisting of 3 $\mu$m thick Au:$SiO_xN_y$ nanocomposite film was confirmed at the telecommunication wavelength of 1550 nm by prism coupler method and compared with the case of using $SiO_2$ matrix. The use of $SiO_xN_y$ matrix provides an effective way of controlling the mode confinement while maintaining or even enhancing the surface plasmon resonance properties.

Experimental investigation on heat transfer of nitrogen flowing in a circular tube

  • Chenglong Wang;Yuliang Fang;Wenxi Tian;Guanghui Su;Suizheng Qiu
    • Nuclear Engineering and Technology
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    • v.56 no.2
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    • pp.463-471
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    • 2024
  • Average and local convective heat transfer coefficients of nitrogen are measured experimentally in an electrically heated circular tube for a range of Reynolds number from 1.08 × 104 to 3.60 × 104, and wall-to-bulk temperature ratio from 1.01 to 1.77. The exit Mach number is up to 0.17, and the heat flux is up to 46 kW·m-2. The molybdenum test section has a 62 diameters heated section with an inside diameter of 5 mm and a 30 diameters entrance section to ensure the fully-developed flow. Uncertainty of Nusselt number is less than 1.6 % in this study. The results indicate that the average heat transfer correlations evaluated by both the bulk and the modified film Reynolds numbers agree well with the experimental data. The local heat transfer results based on bulk properties are compared with previous empirical correlations. New prediction correlations are recommended which are significantly affected by the property variation and heated length. The comparison between the proposed correlations and experimental points shows that 88 % of experimental data fall into an error of 10 %, and almost all data are within an error of 20 %.

A Study on the Emissions of Homogeneous Charge Compression Ignition Engine (균질혼합압축점화기관의 배출가스특성에 관한 연구)

  • Han, Sung-Bin;Choi, Gyeung-Ho
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.28 no.3
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    • pp.324-329
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    • 2004
  • As a new concept in engines and a power source for future automotive applications, the HCCI(Homogeneous Charge Compression Ignition) engine has been introduced. Essentially a combination of spark ignition and compression ignition engines, the HCCI engine exhibits low NO$_x$ and PM emissions as well as high efficiency under part load. In this research, a 4 cylinder diesel engine was converted into a HCCI engine, and propane was used as the fuel. The main parameters for this research are fuel flow rate and the temperature of the intake manifold, and the effects of such on a HCCI engine's performance and exhaust was investigated.