• 한국식물학회:학술대회논문집
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• 한국식물학회 1996년도 식물학심포지움 식물호르몬과 신호전달
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• pp.50-63
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• 1996

Nitrogen (N) is an important regulator of the expression of genes involved in carbon and N assimilation pathways in plants by selectively altering the levels of proteins and/or mRNAs. These in C4 plants include genes for such as phosphoenolpyruvate carboxylase, carbonic anhydrase, and pyruvate-Pi dikinase. The C4 genes are regulated in mesophyll cells by N availability both transcriptionally and posttranscriptionally through cytokinins and glutamine as signals. The level of both the signals is up-regulated by N availability: cytokinins in roots and glutamine in leaves. The level of glutamine is controlled by the differential expression by N of glutamine synthetase and ferrdoxin-dependent glutamate synthase genes which locate in the mesophyll cells of C4 plants. The results is discussed as molecular mechanism for the greater N use efficiency of the plants as well as N partitioning is the photosynthetic cells.

• Journal of Microbiology and Biotechnology
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• 제10권4호
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• pp.455-461
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• 2000

Abstract Spirulina platensis was grown in SWlUe waste to reduce inorganic compowlds and simultaneously produce feed resources. Spirulina platensis prefers nitrogenous compounds in Ibe order: $NH_4^{+}-N>NO_3^{-}-N>simple-N$ such as urea and simple amino acids. It even consumes $NH_4^{+}-N$ first when urea or nitrate are present. Therefore, the content of residual $NH_4^{+}-N$ in Spimlina platensis cultures can be determined by the relative extent of the following processes: (i) algal uptake and assimilation; (ii) ammonia stripping; and (iii) decomposition of urea to NH;-N by urease-positive bacteria. The removal rates of total nitrogen ffild total phosphorus were estimated as an indicator of the treatment effIciency. It was found that Spirulina platensis was able to reduce 70-93% of $P_4^{3-}-P$, 67-93% of inorganic nitrogen, 80-90% of COD, and 37-56% of organic nitrogen in various concentrations of swine waste over 12 days of batch cultivation. The removal of inorganic compounds from swine waste was mainly used for cell growth, however, the organic nitrogen removal was not related to cell growlb. A maximum cell density of 1.52 dry-g/l was maintained with a dilution rate of 0.2l/day in continuous cultivation by adding 30% swine waste. The nitrogen and phosphorus removal rates were correlated to the dilution rates. Based on the amino acid profile, the quality of the proteins in the Spirulina platensis grown in the waste was the same as that in a clean culture.ulture.

• 한국물환경학회지
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• 제23권5호
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• pp.607-612
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• 2007

In this study, we investigated the variations of the kinetic coefficients and Chemical Oxygen Demand (COD), N and P mass used for assimilation of a sequencing batch reactor (SBR) system with the variation of SRTs; SRTs of 7.5, 10.0, 12.5, 15.0 and 20.0 days were tested in one cycle of SBR operation to determine the optimum conditions for the operation of the SBR and estimate its COD, nitrogen and phosphorus removal efficiencies. The SBR system was operated under the conditions as follows: an operation time of 6 hours per cycle, a hydraulic retention time (HRT) of 12 hours, an influent COD loading of $0.4kg/m^3/day$, and an influent nitrogen loading of $0.068kgT-N/m^3/day$. The yield coefficient (Y) and decay rate coefficient ($k_d$) were estimated to be 0.4198 kgMLVSS/kgCOD and $0.0107day^{-1}$ by calculating the removal rate of substrate according to the variation of SRT. Considering total nitrogen amount removed by sludge waste process, eliminated by denitrification, and in clarified water effluent with reference to 150 mg/cycle of influent nitrogen amount, the percentage of nitrogen mass balance from the ratio of the nitrogen amount in effluent (N output) to that in influent (N input) for Runs 1~5 were 95.5, 97.0, 95.5, 99.5, and 95.5%, respectively, which is well accounted for, with mass balances close to 100%.

• 한국토양비료학회지
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• 제1권1호
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• pp.81-87
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• 1968

대두(大豆)의 탄수화물집적과정(炭水化物集積過程)에는 일정(一定)한 rhythm이 있다는 것을 명확(明確)히 하였지만 질소대사(窒素代謝)도 이에 따라 큰 변동(變動)이 있다는 것도 명백(明白)하여졌다. 시비시기(施肥時期)의 조만(早晩)에 불구(不拘)하고 상술(上述)한 결론(結論)은 이용(利用)될 수 있으나 만기시비(晩期施肥)의 경우는, 수확기(收穫期)까지의 시기(時期)가 짧아 정상적(正常的)인 발육(發育)이란 볼 수 없이 생애(生涯)를 끝마친다는 뜻이 되어 수량(收量)에도 매우 큰 영향(影響)을 주는 결과(結果)가 된다. 일반적(一般的)으로 생육(生育)의 전반(前半)은 질소동화기(窒素同化期)이고 후반(後半)은 탄수화물동화기(炭水化物同化期)라고 말할 수 있다. 그러나 시비(施肥)의 조만(早晩)은 이 기간(期間)의 구별(區別)을 비교적(比較的) 지연시켰다. 즉(卽) 전반기(前半期)에 있어서의 탄수화물(炭水化物)의 부족(不足)은 단백질합성(蛋白質合成)의 과정(過程)을 조해(阻害)시켰고, 후반기(後半期)에서의 체내단백질(體內蛋白質)의 부족(不足)은 왕성(旺盛)하여야 할 광합성(光合成)을 감약(減弱)시켜 가하(加何)튼 수량(收量)을 저하(低下)시키는 결과(結果)를 냈다. 따라서 착협기(着莢期)에 있어서의 체내단백질(體內蛋白質)의 부족(不足)은 결정적(決定的)으로 후반기(後半期)에서의 생육부진(生育不振)은 초래(招來)시켰다. 따라서 수량(收量)을 증가(增加)시키기 위(爲)해서는 역시 비교적(比較的) 빠른 시기(時期)에 시비(施肥)하여야 할 것이다. 또 생육과정중(生育過程中)에서의 각종형태(各種形態)의 질소(窒素)의 소장(消長)을 가용성질소(可溶性窒素)로 종합(綜合)하여 질소대사(窒素代謝)의 과정(過程)도 추정(推定)하였다.

• 한국수산과학회지
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• 제37권1호
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• pp.24-32
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• 2004

A mathematical model is used to investigate nitrogen dynamics in the intensive aquaculture ponds in the western coast of Korea. Parameters associated with water quality, sediments and growing of shrimp (Fenneropenaeus chinensis) are measured to calibrate the model for feeding ponds A and B and storage ponds. The model describes the fate of nitrogen including loadings of ammonia from feeds, phytoplankton assimilation, nitrification, sedimentation, volatilization and discharge. The model obtains good agreements with the measured values of TAN $(NH_4,\;NH_3),\;NO(NO_2,\;NO_3)$ and Chl (chlorophyll a). Impacts of water exchange on TAN and Chl are investigated, showing that the range of 0.01-0.2 (/day) cannot effectively reduce TAN but reduces Chl. Nitrogen in the ponds A is removed by sedimentation $66\%,$ volatilization $8\%,$ discharge of particulate and dissolved $8\%.$ The pond B shows $56\%\;and\;26\%$ of sedimentation and volatilization, respectively, to yield $10\%.$ decrease and 8c/o increase compared to those in the pond A. While the pond A has larger area (1.02:0.66 ha) and same stocking density (0.025 md./L) at the beginning of culture, the pond B obtains higher stocking density (0.0065:0.0091 md./L), longer feeding period (103:121 day) and resultant higher shrimp production (1.15:2.13 t/ha/cycle) at harvest. This is possibly due to the hydraulic characteristics driven by paddlewheels. At low ratio of the low speed area and the pond area, the rate of sedimentation is high, while the rate of gas exchange is low. Thus, the measurement and model analysis suggest that water quality and shrimp production are positively correlated with the hydraulic characteristics in the shrimp ponds.

• 한국작물학회지
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• 제26권3호
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• pp.257-262
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• 1981

Orchardgrass 초지에 있어서 조성 초기의 질소시비에 대한 생장 및 수량반응과 질소이용성에 관한 기초자료를 얻기 위하여 1979년 4월에 서울대 농대 실험 농장에서 품종 Potomac을 파종하여 연간 100, 200, 400, 800 kg/ha 수준의 질소비료 시용실험을 난괴법 3반복으로 수행하였다. 그 결과는 다음과 같았다. 1. 파종 첫해의 최대 건물수량 및 최대 질소회수율을 보인 수준은 200kg.N/ha.year였으나 다음 해에는 400kg.N/ha.year였다. 2. 재생기간중의 엽면적지수(LAI)와 순동화율(NAR) 및 체내 전질소함량은 질소증시와 함께 증가했으며 최대 작물생장속도(CGR)는 LAI 5 정도에서 얻어졌다. 3. 채내 ${NO_3}^-$-N 농도는 연간 200kg.N/ha 수준 이상에서 급격히 증가하여 800kg.N/ha 수준에서 중독위험치(1,500ppm ${NO_3}^-$-N)를 초과하였다.

• Asian-Australasian Journal of Animal Sciences
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• 제11권5호
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• pp.608-619
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• 1998

The small and the large intestine of swine represent the organs that extract nutrients from feedstuffs through digestion and fermentation and that allow their absorption and incorporation into the blood circulation. Special attention is directed towards the small intestine of young pigs since the transition to a solid diet at weaning exerts major impacts on the structural and functional integrity of the small intestine. Dietary factors involved in postweaning changes of gut morphology and biochemistry such as removal of bioactive compounds in sows milk at weaning, anti-nutritional factors in weaner diets, dietary fiber and the role of voluntary feed intake will be elucidated. The microbial function of the large intestine which is carried out by a diverse population of microorganisms is dependent on substrate availability. Short chain fatty acids as main fermentation products contribute to the energy supply of the host but they are also important for the maintenance of the morphological and functional integrity of the epithelium in the colon. As a result of bacterial nitrogen assimilation in the large intestine, nitrogen is shifted from the urinary to the fecal excretion route thus saving metabolic energy to the pig because less ammonia would become available for conversion to urea.

• Asian-Australasian Journal of Animal Sciences
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• 제5권4호
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• pp.699-707
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• 1992

Three goats were fed with $^{15}N$-labelled rice straw to study the characteristics of digestion, assimilation, metabolism and excretion of C and N compounds from rice straw. It was shown that the amount of $^{15}N$ assimilated into the bodies of the two slaughtered goats accounted for 38.5 and 23.6% of the total amount of $^{15}N$ ingestion in the experimental diet by each goat. The $^{15}N$ excreted via the feces and urine for the three goats accounted for 34.8, 33.2 and 33.9% of the total amount of $^{15}N$ ingested in the feed of the 3 goats. The recoveries of $^{15}N$ from the two slaughtered goats were 73.3 and 57.5%, with the corresponding rates of $^{15}N$ loss 26.7 and 42.5% respectively. The amino acids digestibilities were 68.7 and 54.0%, while the digestibilities of carbohydrates were 74.8 and 67.7% respectively for goats 1 and 3.

• Asian-Australasian Journal of Animal Sciences
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• 제6권4호
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• pp.619-622
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• 1993

Rumen bacterial amino acids in sheep on urea diet were monitored to assess a possible change in amino acid synthesis as a long term response to high rumen ammonia environment. A sheep was fed a semipurified diet with soybean meal, followed by a diet with urea as a main nitrogen source. Mixed rumen bacteria were harvested from ruminal fluid taken 3 h after feeding (twice in soybean meal feeding and 6 times in urea feeding) and fractionated as cell wall, proteins and protein-free cell supernatant of monitor amino acids in each fraction. Ruminal ammonia concentration at the sampling ranged from 5.7 to 39.5 mgN/dl. Cell wall and protein fractions of mixed rumen bacteria were stable in their amino acid composition regardless of nitrogen sources of diet and the feeding duration. However, protein-free cell supernatant fraction showed a higher alanine proportion with urea feeding (18.6 and 28.2 molar % of alanine for samples from sheep fed soybean meal and urea, respectively) and its duration (20.6 and 32.9 molar % for samples from sheep on urea diet for 1 and 65 days, respectively). Total free amino acid level of bacteria was depressed in the initial period of urea feeding but restored on 65th day of the feeding. These results suggest that an alanine synthesizing system may develop in rumen bacteria as urea feeding becomes longer.

• Fisheries and Aquatic Sciences
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• 제21권8호
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• pp.28.1-28.12
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• 2018

Intertidal macroalgae are exposed to many abiotic stress factors, and they must regularly react to changes in their environment. We used RNA-seq to describe how Porphyra umbilicalis (Rhodophyta) changes gene expression patterns to interact with different habitats. Tissue samples were taken from a typical habitat along the open-coast of the Northwest Atlantic, as well as from a rare, atypical habitat in an estuarine tidal rapid environment. Differential gene expression analyses suggest that pathogic bacteria and viruses may be a significant factor influencing the transcriptome in the human-impacted estuarine environment, but the atypical habitat does not necessarily induce more stress in Porphyra umbilicalis growing there. We found genes related to nitrogen transport are over-expressed in tissue from the open-coastal site compared to those from the estuarine site, where environmental N levels approach hypertrophic levels. Low N levels impede growth, but high levels are toxic to cells, and we use qPCR to show this species regulates expression of a putative high-affinity $NH_4{^+}$ transporter under low and high N conditions. Differences in expression of this transporter in these habitats appear to be inherited from parent to offspring and have general implications for adaptation to habitat in other species that are capable of asexual reproduction, as well as more specific implications for this species' use in aquaculture.