• Title/Summary/Keyword: Nitrogen assimilation

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EFFECTS OF UREA NITROGEN ON THE METABOLISM OF PLANTS (1) Studies on Nitrogen Absorption and Metabolism in Sunflower Leavessprayed with Urea Solution

  • KIM, Joon Ho
    • Journal of Plant Biology
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    • v.4 no.2
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    • pp.51-61
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    • 1961
  • In order to detect the way of absorption and metaboism of the urea it is sprayed on the surface of the leaves of sunflower. The sunflowers used in this study are grown in different conditions such that the one in nittogen aboundant and the other in nitrogen deficient soil, respectively. The urea-N, ammonia-N, amide-N, and 80% alcohol soluble-N in the leaves were quantitatively determined. All of the nitrogenous components measured are generally tended to increased with rising the concentration of urea except only amide-N at 24 hours after sprayed, and these were highly significances. It seemed that hydrolizing of urea into ammonia and carbon dixide and the assimilation of ammonia into other organic nitrogenous constituents were rapid in the young leaves than in the mature. It is interest that the amide content, in the young leaves and nitrogen defieient one were enhanced with the increasing concentration of urea, although in the mature leaves it did not show any change in the urea treatment. It is presumed that the assimilation rate of ammonia and the urease activity were lower in the matture leaves than in the young and nitrogen deficient leaves. No significance at 5% level showed all of the nitrogenous components except total nitrogen between nitrogen abundant and deficent leaves. Urea content was a high peak at first 12 hours, ammonia at 48 hours, and amide and alcohol soluble nitrogen at 96 hours, whence decrease4d the content of these constituents gradually. The total nitrogen content is not incrased obviously by only one time of urea spray in this study. When the concentration of urea was relatively high there appeared the wilting spots on t도 edge of leaves. As a whole, it seemed that sprayed urea was rapidly absorbed and taken part in nitrogen metabolism within relatively short period.

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Effect of Inabenfide and Nitrogen Top-Dressing on Ethylene Evolution and Lodging in Rice (질소추비와 생장조정제 Inabenfide 처리가 벼 도복 및 에틸렌 발생에 미치는 영향)

  • Lee, Sang-Chul;Takeshi, Tanabe
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.38 no.5
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    • pp.383-390
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    • 1993
  • This experiment was carried out in the green house at Tokyo University of Agriculture in 1992 to investigate the relationship between lodging resistances and specific morphological characters and to examine the effects of nitrogen top-dressing and growth retardant on changes of the endogenous ethylene content in rice plant. Inabenfide did not affect significantly yield, yield component, chlorophyll content, and $CO_2 assimilation but, decreasing plant height when growth regulators were applied at booting stage, decreasing internode elongation, and increasing culm thickness and diameter of the third and fourth internode. While, nitrogen top-dressing usually showed to effect increasing vegetative growth such as plant height, tiller number, and morphological characteristics but, were not significantly affected the chlorophyll content and $CO_2 assimilation. Ethylene evolution was dramatically changed with nitrogen top-dressing and growth retardant. Application of Inabenfide at 15 days before heading inhibited ethylene evolution in the all nitrogen levels. Higher nitrogen top-dressing showed higher ethylene evolution than that of low nitrogen level. High positive correlation was detected between nitrogen application and ethylene evolution.

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Effect of simazine on nitrogen assimilation of rhodopseudomonas sphaeroides (Rhodopseudomonas sphaeroides의 질소 동화작용에 미치는 simazine의 영향)

  • 이혜주;이진애;박인호;선우양일
    • Korean Journal of Microbiology
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    • v.27 no.3
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    • pp.304-309
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    • 1989
  • The effects of simazine [2-chloro-4, 6-bis(methylamino)-s-triazine] on in vivo nitrogenase, glutamine synthetase, glutamate synthase, and glutamate dehydrogenase which are important in nitrogen assimilation of Rhodopseudomonas sphaeroides were investigated. Simazine inhibited the synthesis of nitrogenase and glutamine synthetase. The activity of glutamine synthetase in crude extracts of Rhodopseudomonas sphaeroides is less inhibited by simazine than that of glutamate synthase and glutamate dehydrogenase. These results suggest the possibility that simazine inhibits photosynthetic activity and so inhibits the synthesis of nitrogenase and glutamine synthetase in Rhodopseudomonas sphaeroides.

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Nondestructive Nutrient Diagnosis for Nitrogen with Specific Color Difference Sensor(SCDS) in Hydroponics of Tomato (Lycopersicon esculentum MILL.) (토마토 양액 재배시 비파괴 간이 질소 영양 진단)

  • 이용범;노미영;조영렬;배종향
    • Journal of Bio-Environment Control
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    • v.4 no.2
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    • pp.175-180
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    • 1995
  • This study was conducted to establish the nondestructive nutrient diagnosis method for nitrogen in tomato leaf using SCDS(specific color difference sensor). NFT(nutrient film technique) system was used in this experiment and nitrogen concentrations treated in nutrient solution were 0, 10, 50, 100, 150, 200, 300 and 600ppm. As nitrogen concentration in nutrient solution was increased from 0ppm to 150ppm, the stomatal resistance of tomato leaf was decreased abruptly, the $CO_2$ assimilation rate was increased but there was no big difference in the range of 100-500ppm. As the SCDS value of tomato leaf was increased, the $CO_2$ assimilation rate was increased linearly but the total average fruit weight and marketable yield were increased quadratically. The $CO_2$ assimilation rate was largely increased in the 0-3% range of leaf nitrogen content, but photosynthetic saturation was shown in 3.3-3.5%. The leaf nitrogen content was closely related to SCDS value of tomato leaf. Considering physiological activity, growth and yield of tomato, the optimum ranges of leaf nitrogen content were found to be 3.0-3.8% and the SCDS values equivalent for those ranges were 40.0-52.2.

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Nitrogen Utility during the Population Development with Different Clipping Treatments on Italian Rye Grass Field (Italian Rye Grass 초지군락의 청예처리빈도에 따른 생산성과 질소이용성)

  • 송승달
    • Journal of Plant Biology
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    • v.22 no.3
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    • pp.63-69
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    • 1979
  • The plant growth and net production, the nitrogen uptake and recycling, the nitrogen mobility and allocation to each organ, and the nitrogen utility from the Italian rye grass field during the population development were analyzed in comparison with different clipping treatments. The maximum dry matter standing crop and nitrogen quantity of harvest increased significantly, however, the annual amounts of dry matter and nitrogen assimilation showed little variations with increasing clipping frequencies. Plants treated with frequent clippings allocated relatively more nitrogen to leaves and less to roots during the experimental period. The amount of recycling of nitrogen decreased considerably due to frequent clippings. The annual averages of nitrogen utility indices changed in inverse relation to the nitrogen availability; such as 63, 58, 44 and 35 for C, A, M and J plots, respectively.

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Effect of High Nitrogen Application on Two Components of Dark Respiration in a Rice Cultivar Takanari

  • Akita, Shigemi;Lee, Kwang-hong
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.47 no.4
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    • pp.323-327
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    • 2002
  • Plant growth and the two components of respiration, growth and maintenance, were compared between low and high nitrogen applications in hydroponic culture on a high-yielding rice cultivar 'Takanari' (Oryza sativa L.). Grain yield decreased by high nitrogen application, and thus this cultivar has low adaptability to nitrogen. Growth efficiency (GE) and net assimilation rate (NAR) were lower in the high-nitrogen plot. The maintenance coefficient (m) and growth coefficient (g) of dark respiration were 0.0111 $d^{-1}$ and 0.196 in the low-nitrogen plot and 0.0166 $d^{-1}$ and 0.237 in the high-nitrogen plot, respectively. Thus, high nitrogen application increased both g and m. Calculated $R_m$ (maintenance respiration rate) was 70 and 90% of total respiration rate at heading, respectively. The significance of nitrogen adaptability and g was discussed.

Changes in the Nitrate Assimilation and Ascorbic Acid Content of Spinach Plants Treatmented with Nutrient Solutions Containing High Nitrogen and Low Potassium (고질소 및 저 칼륨 양액처리시 시금치내의 비타민C및 질소 대사의 변화)

  • Park, Yang-Ho;Seo, Beom-Seok
    • Korean Journal of Soil Science and Fertilizer
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    • v.42 no.4
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    • pp.301-306
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    • 2009
  • This study was conducted to determine the physiological differences betweenhealthy and wilted plants with respect to nitrate assimilation and ascorbic acid content. Wilting was artificially induced in spinach plants by treating the seeds with nutrient solution containing high nitrogen and low potassium. The plants were cultured in different plots 4 types of media: 1N-1P-1K (control), 6N-1P-0K (0K), 6N-1P-0.5K (0.5K), and 6N-1P-2K (2K). The rate of wilting among the plants was as follows: control, 0%; 2K, 10%; 0.5K, 40%; and 0K, 70%. This shows that under high nitrogen conditions, the lower the amount of potassium provided, higher was the rate of wilting. There were no differences in plant growth among the plants treated with different levels of potassium under high nitrogen conditions.The nitrate content in both the leaves and the roots was higher in plants grown under high nitrogen media than those in the control. Furthermore, the nitrate level decreased with increasing potassium concentration. The ascorbic acid content of spinach under high nitrogen conditions was lower than those of the control.

The Contribution of Molecular Physiology to the Improvement of Nitrogen Use Efficiency in Crops

  • Hirel, Bertrand;Chardon, Fabien;Durand, Jacques
    • Journal of Crop Science and Biotechnology
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    • v.10 no.3
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    • pp.123-132
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    • 2007
  • In this review, we discuss the ways in which our understanding of the controls of nitrogen use efficiency applied to crop improvement has been increased through the development of molecular physiology studies using transgenic plants or mutants with modified capacities for nitrogen uptake, assimilation and recycling. More recently, exploiting crop genetic variability through quantitative trait loci and candidate gene detection has opened new perspectives toward the identification of key structural or regulatory elements involved in the control of nitrogen metabolism for improving crop productivity. All together these studies strongly suggest that in the near future nitrogen use efficiency can be improved both by marker-assisted selection and genetic engineering, thus having the most promise for the practical application of increasing the capacity of a wide range of economically important species to take up and utilize nitrogen more efficiently.

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The effect of dry matter allocation at the early seedling stare on the growth of two grass species (두 초본 종의 생장에 있어서 실생 초기의 물질분해 효과)

  • Park, Young-Mok
    • The Korean Journal of Ecology
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    • v.15 no.3
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    • pp.297-309
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    • 1992
  • The growth of digitaria adscendens and eleusine indica, annual weeds common in japan, was experimentally analyzed under varying untrient conditions. compared with e. indica, d. adscendensshowed a higher the two under high nitrogen availability. Higher net assimilation rate(nar) in d. adscendens than e. india was responsible for high RGR in D. adscendens under the low nitrogen availability. The different of NAR in the two species was induced not by leaf nitrogen content but by nitrogen use efficiency. Under unfertilized conditions nitrogen uptake rate(nur) was greater in d. adscendens than e. indica. Specific absorption rate in two species was similar, but root mass was greater in d. adscendens than e. indica.d. adscendens allocated more dry matter to roots than e. india earlier stage of seedlings. The contributed to higher rot mass and in turn resulted in higher nur in d. adscendens than that in e. india. It is concluuded that the larger allocation of dry matter to roots at early seedling stage in d. adscendens plays an important role in obtaining nitrogen for the continuation of growth under low nitrogen availability.

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Effect of Organic Fertilizer and Mulch Sources on Growth and CO2 Assimilation in MM.106 Apple Trees (유기질 비료와 멀칭자재가 MM.106 사과 대목의 생장과 광합성에 미치는 영향*)

  • Choi, Hyun-Sug;Rom, Curt;Kim, Wol-Soo;Choi, Kyeong-Ju;Lee, Youn
    • Korean Journal of Organic Agriculture
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    • v.18 no.2
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    • pp.245-255
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    • 2010
  • The study was conducted to investigate the effects of organic fertilizers and mulches on the growth and $CO_2$ assimilation in MM.106 apple trees. Growth and $CO_2$ assimilation of MM.106 apple trees grown in a greenhouse were affected by the nutrient concentrations and carbon (C) and nitrogen (N) ratio in the raw materials of organic fertilizers and mulches. The optimum C:N ratios, which makes microorganism convert the organic N into the inorganic N, were obtained in the organic fertilizer, poultry litter, green compost, and grass clippings, resulting in increasing single shoot height, SPAD, and $CO_2$ assimilation. The SPAD and $CO_2$ assimilation were affected by the treatments 5 weeks after the treatments, and then the tree growth was affected by the treatments 6 weeks later. The most efficient tree growth and development were observed in the 10 to $15\;mg{\cdot}kg^{-1}$ of the inorganic N in a soil, and the N was strongly related to the tree growth and development.