• Title/Summary/Keyword: Nitrogen Uptake

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Respiration, $alpha-Naphthylamine$ Oxidizing Ability and TTC Reducing Ability of Roots and Uptake of Water and Nutrients and Bleeding as Affected by the Level of Nitrogen and Phosphorous Application Shading, Water Potential and Temperature in the Rice Plants (벼 뿌리의 호흡, $\alpha-Naphthylamine$, 산화력, TTC 환원력, 양수분 흡수 및 일필에 대한 질소 및 인산시용량, 차광, 수분 Potential 및 온도처리의 영향)

  • Kwon Yong Woong;Na Ae Sil;Lee Min Kyu
    • Proceedings of the Korean Society of Crop Science Conference
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    • 1988.07a
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    • pp.16-17
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    • 1988
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Characterization of Nitrate Uptake Mediated by Soil Bacterial Strains

  • Ahn, Hae-Jin;Cho, Kwang-Hyun;Kim, Young-Kee
    • Proceedings of the Korean Biophysical Society Conference
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    • 2002.06b
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    • pp.42-42
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    • 2002
  • Salt accumulation in agricultural soils reduces the growth and productivity of crops. Although scientists have been studied the impacts of salinity on plants, the physiological mechanism of adaptation to salinity has not been well understood. Practically, salinity in irrigated soil of green and glass houses keeps increasing in Korea by the massive application of nitrogen fertilizer.(omitted)

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Regulation of extracellular alkaline protease biosynthesis in a strain of streptomyces sp. (Streptomyces sp. 일주에서 균체외 호염기성 단백질분해 효소의 생합성 조절)

  • 신현승;이계준
    • Korean Journal of Microbiology
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    • v.24 no.1
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    • pp.32-37
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    • 1986
  • In fermentation studies it revealed that Streptomyces sp. SMF 3001 started to synthesize extracellular alkaline protease from early exponential phase of cell growth. The biosynthesis of the alkaline protease was greatly induced by skim milk as a sola nitrogen source and further stimulation was observed under inorganic sulphur limited culture. However, it was found that the biosynthesis was apparently repressed by $NH_4^+$ and free amino acids, specially by cysteine. It was considered that the strain SMF 301 of Streptomyces sp. would produce the alkaline protease for the uptake of sulphur compounds from protein contained in the culture broth.

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Overexpression of Gene Encoding Tonoplast Intrinsic Aquaporin Promotes Urea Transport in Arabidopsis

  • Kim, Sun-Hee;Kim, Kang-Il;Ju, Hyun-Woo;Lee, Ho-Joung;Hong, Suk-Whan
    • Journal of Applied Biological Chemistry
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    • v.51 no.3
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    • pp.102-110
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    • 2008
  • Complementation assay of the urea uptake-defective yeast mutants led to the identification of the Arabidopsis AtTIP4;1 gene encoding the aquaporin. However, its physiological functions still remain elusive. In the present study, histochemical and genetic analyses were performed to understand the physiological roles of AtTIP4;1 in urea uptake. The AtTIP4;1 product was detectible in the roots, but not in the leaves, the stem, and the flower. Its promoter allowed the expression of the $\beta$-glucuronidase reporter gene in the roots and the apical meristem in Arabidopsis. The AtTIP4;1 products were induced under nitrogen-deficient conditions. To investigate the role of the tonoplast intrinsic protein in urea transport and developments, Arabidopsis with the loss- and the gain-of-function mutations by T-DNA insertion in AtTIP4;1 and 35S promoter-mediated overexpression of AtTIP4;1 were identified, respectively. The transfer DNA insertion and the AtTIP4;1-overexpressed plants showed normal growth and development under normal or abiotic stress growth conditions. The urea-uptake studies using $^{14}C$-labeled urea revealed higher accumulation of urea in the AtTIP4;1-overexpressed plants. These results provide evidence that overexpression of AtTIP4;1 leads to the increase in the urea-uptake rate in plants without detectable defects to the growth and development.

Characteristics of Nutrients Removal Process Activating Soil Microorganisms and Phosphorus Uptake under Anoxic Condition(II) (토양미생물을 활성화한 영양염류 제거 공정의 특성과 무산소 조건에서의 인 섭취(II))

  • Shin, Eung-Bae;Ko, Nam-Ho
    • Journal of Korean Society of Environmental Engineers
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    • v.22 no.10
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    • pp.1757-1763
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    • 2000
  • To consider the nutrient removal characteristics of BNR process activating soil microorganisms under the influence of DPB and to clear the characteristics of DPB under anoxic condition was investigated in the this study. The batch tests were conducted using sludge sampled from the BNR process activating soil microorganisms during operation periods. The results of this study were summarized as follows: - The DPB(Denitrifying Phosphorus removing Bacteria) performing denitrification and phosphorus uptake in the anoxic phase plays an important role in removing nitrogen and phosphorus in the BNR process activating soil microorganisms. - The PUR(Phosphorus Uptake Rate) of DPB in the anoxic phase was to be about 50% of PUR in the aerobic phase. - The DPB in the BNR process turned out to be increasing nutrient removal efficiency of BNR process.

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A study on advanced treatment of domestic wastewater nutrient removal by using Biosorption (생흡착을 이용한 생활하수의 염양염류제거에 관한 고도처리 연구)

  • Park, Ju-Seok;Kim, Hyun-Kab;An, Chang-Hwan;Whang, Jung-Ki;Ahn, Sang-Jun
    • Journal of Korean Society of Water and Wastewater
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    • v.13 no.3
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    • pp.29-35
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    • 1999
  • The purpose of this study is to remove the organics, nitrogen and phosphorus using biosorption for the domestic sewage. The new process using biosorption is based on the methods of contact-stabilization, which remove the organics by absorbing them to the surface of the microorganism in the activated sludge. This process consists of biosorption reactor, biosorption clarifier, nitrifying reactor, nitrifying clarifier, denitrifying reactor, phosphorus uptake(polishing) reactor and final clarifier. The efficiency of removal could be reached 91% for organics, 76% for nitrogen, 90% for phosphorus in Eujungbu pilot plant. We operated the plant which irrigated $10m^3$ per day for sewage. During our operation the HRT(Hydraulic Retention time) was maintained for 10.5hr, but it could be reduced as 8.5hr according to our operation results.

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Cencept of adaptability for heavy nitrogen in view of nitrogen metabolism -II. Nitrogen metabolism under the change of itrogen nenvironment (내비성(耐肥性)에 관(關)한 질소대사적(窒素代謝的) 개념(槪念) -II. 질소환경(窒素環境) 변화(變化)에 따른 질소대사(窒素代謝))

  • Yoon, Jong Hyuk;Park, Hoon;Cho, Sung Jin
    • Korean Journal of Soil Science and Fertilizer
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    • v.7 no.1
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    • pp.49-53
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    • 1974
  • Varietal difference in nitrogen metabolism was investigated under water culture system with high (50 or 80ppm) and low (10 or 40 ppm) levels of nitrogen and with two-week minus nitrogen treatment at maximum tillering and heading stage using a leading local variety, Jinheung and high-yielding IR667 line (newly bred tropical variety). 1. In high nitrogen level Jinheung showed higher yield than IR667. vise versa in low nitrogen level. 2. Poor yield of IR667 at high nitrogen may be due to ammonium toxicity that was eliminated by minus nitrogen from culture solution. 3. IR667 was more sensitive to the change of nitrogen environment. 4. With high nitrogen medium Jinheung showed nigher nitrogen uptake and higher capacity of protein synthesis than IR667, and vise versa at low nitrogen medium. 5. From the above facts it could be concluded that Jinheung has higher metabolic adaptability for heavy nitrogen while IR667 has higher structural adaptability for heavy mitrogen and that better productive adaptability will be resulted in the combination of both characteristics.

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Kinetics of Removing Nitrogenous and Phosphorus Compounds from Swine Waste by Growth of Microalga, Spirulina platensis

  • Kim, Min-Hoe;Chung, Woo-Taek;Lee, Mi-Kyung;Lee, Jun-Yeup;Ohh, Sang-Jip;Lee, Jin-Ha;Park, Don-Hee;Kim, Dong-Jin;Lee, Hyeon-Yong
    • Journal of Microbiology and Biotechnology
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    • v.10 no.4
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    • pp.455-461
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    • 2000
  • Abstract Spirulina platensis was grown in SWlUe waste to reduce inorganic compowlds and simultaneously produce feed resources. Spirulina platensis prefers nitrogenous compounds in Ibe order: $NH_4^{+}-N>NO_3^{-}-N>simple-N$ such as urea and simple amino acids. It even consumes $NH_4^{+}-N$ first when urea or nitrate are present. Therefore, the content of residual $NH_4^{+}-N$ in Spimlina platensis cultures can be determined by the relative extent of the following processes: (i) algal uptake and assimilation; (ii) ammonia stripping; and (iii) decomposition of urea to NH;-N by urease-positive bacteria. The removal rates of total nitrogen ffild total phosphorus were estimated as an indicator of the treatment effIciency. It was found that Spirulina platensis was able to reduce 70-93% of $P_4^{3-}-P$, 67-93% of inorganic nitrogen, 80-90% of COD, and 37-56% of organic nitrogen in various concentrations of swine waste over 12 days of batch cultivation. The removal of inorganic compounds from swine waste was mainly used for cell growth, however, the organic nitrogen removal was not related to cell growlb. A maximum cell density of 1.52 dry-g/l was maintained with a dilution rate of 0.2l/day in continuous cultivation by adding 30% swine waste. The nitrogen and phosphorus removal rates were correlated to the dilution rates. Based on the amino acid profile, the quality of the proteins in the Spirulina platensis grown in the waste was the same as that in a clean culture.ulture.

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