• Preventive Nutrition and Food Science
• /
• 제14권3호
• /
• pp.195-200
• /
• 2009

This study was carried out to investigate the biological activities of star anise extracts obtained from four different solvents (water, methanol, ethanol and chloroform) by measuring total polyphenol and flavonoid contents, DPPH radical scavenging, hydroxyl radical scavenging, nitrite scavenging activity and antimicrobial activity. The methanol extract showed the highest extraction yield, followed by ethanol, water and chloroform. The properties of the extracting solvents significantly affected the total polyphenol content. Methanol extracts contained more total polyphenols than any other extracts. The highest DPPH radical scavenging activity was found in methanol extract. The hydroxyl radical and nitrite scavenging activity were highest in methanol and ethanol extracts. In antimicrobial activity, water extract showed stronger activities than methanol and ethanol extract against Micrococcus luteus and Bacillus subtilis, and no inhibitory effects on Gram negative bacteria were found in all extracts at the concentration used.

• Food Science and Biotechnology
• /
• 제15권3호
• /
• pp.351-356
• /
• 2006

In this study, we investigated the effect of adding a 1, 3, or 5% solution of germinated brown rice concentrate (GBRC) to fermented kimchi. During fermentation, the concentration of free amino acids and essential amino acids increased with increasing concentrations of GBRC. In particular, higher levels of free amino acids were associated with a sweet taste compared with controls. The ${\gamma}$-aminobutyric acid (GABA) content of kimchi containing the 5% GBRC solution was 3 times higher than that of controls. The total phenolic compound content (130 mg%) did not change significantly in the control group, but increased in 10 mg% increments as the GBRC concentration rose from 1 to 3 to 5%. 2,2-diphenyl-1-picryhydrazyl (DPPH) free radical scavenging activity and superoxide radical scavenging activity also increased with the GBRC concentration, with maximum activity during the ripe stage with GBRC measured at 79 to 82% compared with controls (30 to 71 %). The nitrite scavenging activity was 10% higher with GBRC compared with controls and was highest when the pH was 1.2. These results showed that the addition of GBRC is effective in improving the function of kimchi.

• 동의생리병리학회지
• /
• 제22권3호
• /
• pp.654-659
• /
• 2008

To confirm possibility of Nelumbo nucifera leaves as biofunctional material, we investigated the antioxidant activity and nitrite scavenging ability of ethanol extract from Nelumbo nucifera leaves. Nelumbo nucifera leaves were extracted with ethanol and concentrated under vacuum using rotary evaporator. Then, antioxidative activity and nitrite scavenging ability of the extract were examined in vitro. Electron -donating ability of the extract at RC50 was 90.19 ${\mu}g/mL$. After addition of 0.96 mg/mL, autooxidation of pyrogallol decreased to 66.19% by superoxide dismutase-like activity. In antioxidative activity of the extract against linoleic acid during incubation times of 24, 48, and 96 hours at $40^{\circ}C$, lipid peroxidation values significantly decreased to 72.53%, 82.00%, 84.69% with addition of 0.2 mg/mL, respectively. Total phenolic content was determined as gallic acid equivalents (GAE) and the value revealed to be $282.84\;{\pm}\;9.03$ GAE ${\mu}g/mg$ of the extract. Nitrite scavenging ability showed the most remarkable effect at pH 1.2, exhibited to 45.55% by addition of 0.2 mg/mL. These results suggest that ethanol extract from N. nucifera leaves can be used as bioactive and functional material.

• 한국식품조리과학회지
• /
• 제16권4호
• /
• pp.372-377
• /
• 2000

Applemint, geranium, lemonbalm, rosemary, sage, thyme등 6종 herb의 기능성을 검토하기 위하여 물 및 95% methanol로 추출하여 얻은 추출액의 전자공여능, 항균작용 및 아질산염 소거능을 조사하였다. 전자공여능은 60.7∼92.2%로 95% methanol추출물이 물추출물보다 높게 나타났고 geranium, sage, lemonbalm, thyme, rosemary, applemint 순으로 작용이 우수하였다. 각종 herb추출물의 항균력은 물보다 methanol추출물이 우수하였고 gram음성균보다는 양성균주에서 효과적이었으며 geranium과 rosemary 공시균주에 대하여 광범위한 항균활성을 나타내었고 분획별 항균력 측정결과 geranium은 극성용매 분획에서, rosemary는 비극성 용매 분획에 항균력이 집중되어 있음을 확인하였다. 아질산염 소거능 역시 물추출물 보다는 methanol추출물에서 높은 활성을 보였고 pH 1.2에서 가장 효과적이었으며 pH가 증가할수록 감소하였다.

• 한국식품영양학회지
• /
• 제34권2호
• /
• pp.181-186
• /
• 2021

Antioxidant properties and antioxidant activities were analyzed for water extracts and 50% and 70% ethanol extracts of the leaf of Angelica gigas Nakai. The polyphenol and flavonoid contents in water, 50%, and 70% ethanol extract of the leaf of Angelica gigas Nakai, it was found that the polyphenol contents were 18.75 mg GAE/g, 28.95 mg GAE/g, and 34.73 mg GAE/g, respectively, and flavonoid contents were respectively. The DPPH IC50 scavenging activity was 45.84 mg/mL, 36.44 mg/mL, 19.11 mg/mL, respectively, and theABTS+ radical scavenging ability (1 mg/mL) was 28.73%, 22.79%, and 12.70%, respectively. Tyrosinase inhibitory activity, 70% ethanol extract, 50% ethanol extract, and water extract 33.14%, and 4.53%, respectively. Nitrite scavenging activity, 70% ethanol extract, 50% ethanol extract, and water extract were in the order of 1 mg/mL scavenging activity, 36.43%, 34.80%, and 18.85%, respectively.

• 한국식품영양학회지
• /
• 제28권2호
• /
• pp.295-301
• /
• 2015

삼채뿌리와 잎이 가진 기능성 식품소재로서의 활용도를 높이고자 다양한 생리활성을 평가하였다. 삼채뿌리의 조사포닌, 폴리페놀, 플라보노이드 함량은 각각 4.28 mg/g, 18.27 mg/g, 0.95 mg/g, 삼채잎의 조사포닌, 폴리페놀, 플라보노이드 함량은 각각 4.17mg/g, 35.91 mg/g, 2.79 mg/g으로 나타났다. 삼채뿌리 및 잎 부위의 70% 에탄올 추출물의 DPPH 라디칼 소거능 $IC_{50}$으로 23.01 mg/mL, 4.82 mg/mL로 나타났으며, hydroxyl radical 소거능 $IC_{50}$ 값이 각각 40.82 mg/mL, 27.24 mg/mL를 나타났다. ${\alpha}$-Amylase 저해 활성은 삼채뿌리 및 잎 부위 에탄올 추출물 10 mg/mL를 처리했을 때 억제활성이 각각 61.38%, 61.01%로 나타났다. 삼채뿌리 및 잎 부위의 아질산염 소거능을 측정한 결과, 삼채추출물의 농도가 증가함에 따라 그 활성 역시 증가하는 경향으로 나타났다. 이상의 결과로 삼채뿌리보다는 삼채잎 부위에 항산화 및 항당뇨 활성이 나타났으며, 삼채잎을 활용한 식품 소재로써 개발할 가치가 매우 높은 것으로 판단된다.

• 한국식품영양과학회지
• /
• 제35권3호
• /
• pp.262-270
• /
• 2006

볶음 처리한 새송이버섯의 환류추출조건에 따른 추출물의 생리기능적 특성을 살펴보았다. 에탄올농도에 따라서는 50% 에탄올농도로 추출하였을 때 총 페놀성 화합물 함량 및 수소공여능이 높은 것으로 나타났으며, 75% 에탄올농도로 추출하였을 때 SOD유사활성이 높은 것으로 나타났다. 아질산염 소거능의 경우에는 에탄올농도가 낮을수록 높은 것으로 나타났다. 추출시간이 증가할수록 수소공여능은 감소하는 것으로 나타났으며, SOD유사활성 및 아질산염 소거능은 증가하는 것으로 나타났다. 추출온도가 증가할수록 수소공여능 및 SOD 유사활성은 감소하는 것으로 나타났으며, 아질산염 소거능은 큰 차이를 보이지 않았다. 용매에 대한 시료량이 증가할수록 수소공여능, SOD 유사활성 및 아질산염 소거능이 감소하는 것으로 나타났다. 이상의 결과를 바탕으로 하여 볶음 처리한 새송이버섯의 추출조건 설정에 활용될 수 있으리라 사료된다.

• Nutrition Research and Practice
• /
• 제1권3호
• /
• pp.189-194
• /
• 2007

This study investigated in vitro antioxidant activity of Sonchus oleraceus L. by extraction solvent, which were examined by reducing power, hydroxyl radical-scavenging activity(HRSA) and 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assays. 70% MeOH extract had the greatest reducing power while EtOH extract had the greatest HRSA. The antioxidant activity of S. oleraceus extracts was concentration dependent and its $IC_{50}$ values ranged from 47.1 to $210.5\;{\mu}g/ml$ and $IC_{50}$ of 70% MeOH, boiling water and 70% EtOH extracts were 47.1, 52.7 and $56.5\;{\mu}g/ml$, respectively. 70% MeOH extract of S. oleraceus contained the greatest amount of both phenolic and flavonoid contents. The extracts tested had greater nitrite scavenging effects at lower pH conditions. The cytotoxic activity showed that EtOH extract had the best activity against the growth of stomach cancer cell. These results suggest that S. oleraceus extract could be used as a potential source of natural antioxidants.

• 한국식품저장유통학회지
• /
• 제9권3호
• /
• pp.351-355
• /
• 2002

목초액의 아질산염 소거, 전자공여작용 및 대두유에 농도별(0.5, 1 및 5%)로 첨가하여 항산화성을 조사하였다. 아질산염 소거 효과는 모든 농도에서 pH가 증가하면서 감소하는 경향을 보였고, pH 1.2의 경우 100 ${\mu}\ell$/mL의 농도에서 80%이상의 높은 활성을 보였다. 전자공여작용은 50 ${\mu}\ell$/mL의 농도에서 약 80%의 효과를 나타내었다. 목초액을 첨가하지 않은 대두유를 control로 사용하였고, 0.1% BHT를 첨가한 대두유는 비교군으로 사용하여 5$0^{\circ}C$에서 10일간 저장하면서 실험을 하였다. 과산화물가는 목초액 1%와 5% 첨가군이 control과 BHT 첨가군보다 낮게 나타났으며, TBA가는 5% 목초액에서 가장 낮았고, 1% 목초액, 0.1% BHT 및 0.5% 목초액 첨가군 순으로 나타났다. 산가는 5% 목초액 첨가군이 가장 낮았고, 0.1% BHT, 1% 및 0.5% 목초액의 순으로 낮게 나타났다.

• 한국식품영양학회지
• /
• 제33권1호
• /
• pp.64-73
• /
• 2020

The purpose of this study was to investigate the antioxidant activity and β-glucan content of extracts extracted by varying the temperature at 30, 55 and 80℃ after hot air drying or freeze drying of Pleurotus ostreatus and Pleurotus eryngii. For the analysis antioxidant activity of each mushroom, β-glucan, total phenol, flavonoid contents, and DPPH·ABTS⁺·Nitrite assay were measured. Also, the β-glucan content, total flavonoid content and ABTS⁺ scavenging activity increased with freeze drying rather than hot air drying, and increased with increasing extraction temperature in both mushrooms. However, the total phenol and nitrite scavenging activity increased with hot air drying rather than freeze drying, and decreased with increasing temperature in both mushrooms. DPPH scavenging activity was not significant in both mushrooms, but decreased with increasing extraction temperature. Pearson's correlations between total flavonoid content and antioxidant activities were r=0.719~0.753 (p<0.01). As a result, the β-glucan content, total flavonoid content, and ABTS radical cation scavenging activity were highest during freeze drying and extraction at 80℃. And the highest total phenol content, DPPH radical scavenging activity and nitrite scavenging activity were obtained during hot air drying and extraction at 30℃.