• Title/Summary/Keyword: Nitrite(${NO_2}^-$)

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In-vitro Antithrombosis Activity of Different Parts of Sorbus commixta from Ulleung Island (울릉도 마가목의 다양한 부위의 항혈전 활성)

  • Kim, Mi-Sun;Seong, Ha-Jung;Sohn, Ho-Yong
    • Journal of Life Science
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    • v.26 no.3
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    • pp.289-295
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    • 2016
  • Sorbus commixta, a flowering plant in the Rosaceae family, is native to Japan and Ulleung Island, Korea. This plant is also called maga-mok or mai-mok in Korea because the bud of the stem has a similar shape to the teeth of a horse. In this study, hot water extracts from different parts of S. commixta, such as leaf, stem, and immature and mature fruits, were prepared, and their antithrombosis and antioxidant activities were evaluated. The extraction yield and pH of stem extracts were 3.99% and 5.5, respectively. The stem extracts contained 89.2 mg/g of total polyphenols and 28.3 mg/g of total flavonoids. The hot water extracts prepared from the leaf, stem, immature, and mature fruit of S. commixta exhibited no hemolytic activity against human red blood cells, up to a concentration of 0.5 mg/ml. In an anticoagulation assay, the stem extracts showed strong extension in thrombin, prothrombin, and activated partial thromboplastin times, whereas the other extracts had no anticoagulation activity. In a platelet aggregation inhibitory activity assay, all the extracts tested had no inhibitory activity against human platelets. With regard to antioxidation activity, the stem extracts showed stronger radical scavenging activity and reducing power activity than the other extracts. The calculated RC50s, the concentration required for 50% radical scavenging activity, for DPPH anions, ABTS cations, and nitrite of the crude stem extracts were 119.7, 53.3, and 117.5 μg/ml, respectively, whereas they were 13.7, 5.2, and 14.9 μg/ml for DPPH anions, ABTS cations, and nitrite, respectively, for vitamin C. The results suggest that the stem extracts of S. commixta have strong potential for use as a novel resource for antithrombosis agents.

Effects of Inhibitors on Corrosion Resistance of Steel in CaCl2 Solution Based on Response Surface Analysis

  • Park, Tae-Jun;Jang, HeeJin
    • Corrosion Science and Technology
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    • v.20 no.3
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    • pp.129-142
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    • 2021
  • Effects of corrosion inhibitors (i.e., sodium nitrite, sodium hexametaphosphate, trimethylamine (TEA), sugar, and urea) on the corrosion resistance of carbon steel in CaCl2 solution were investigated. The test solution was designed with response surface methodology of design of experiments (DOE) in the range of 0 ~ 50 ppm for NaNO2, 0 ~ 200 ppm for (NaPO3)6, 0 ~ 2000 ppm for TEA, 0 ~ 3000 ppm for sugar, 0 ~ 200 ppm for urea with 3 wt% CaCl2. The corrosion potential and the corrosion rate were measured with potentiodynamic polarization tests and analyzed statistically to find main effects of inhibitor concentrations and interactions between them. As a result, hexametaphosphate was the most effective compound in reducing the corrosion rate. Sugar also reduced the corrosion rate significantly possibly because it covered the surface effectively with a high molecular weight. The inhibiting action of sugar was found to be enhanced by adding trimethylamine into the solution. Nevertheless, trimethylamine did not appear to be effective in inhibiting corrosion by itself. However, urea and sodium nitrite showed almost no inhibition on corrosion resistance of steel.

Antioxidant Activity and Inhibitory Effect against Oxidative Neuronal Cell Death of Kimchi Containing a Mixture of Wild Vegetables with Nitrite Scavenging Activity (아질산염 소거 작용을 가진 산채 혼합물을 함유한 김치의 항산화 활성 및 산화적 신경세포 사멸 억제 효과)

  • Kang, Kyung Hun;Park, Si Young;Kwon, Ki Han;Lim, Heekyung;Kim, Sung Hyun;Kim, Jeong Gyun;Chung, Mi Ja
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.44 no.10
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    • pp.1458-1469
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    • 2015
  • This study was carried out to investigate the nitrite scavenging activities (NSA) of nine kinds of wild vegetables in a $NaNO_2$ model system and nitrite of Chinese cabbage as well as the inhibitory effect of kimchi containing a mixture of wild vegetables (MWV) with nitrite scavenging activity on brain neuronal cell death. NSA was higher at pH 1.2 than pH 4.2 in all samples. NSA of extracts from sprouts of Oenothera laciniata and Aster scaber (AS) was above 90% at pH 1.2. AS, Codonopsis lanceolate (CL), Adenophora triphylla (AT), Platycodon grandiflorum (PG), and Taraxacum officinale (TO) extracts showed significantly higher levels of NSA than those from other extracts at pH 4.2. CL, AT, PG, and TO extracts showed high NSA on nitrite of Chinese cabbage. In addition, the effects of MWV on antioxidant and brain neuronal cell death induced by oxidative stress were investigated in human brain neuroblastoma SK-N-SH cells. MWV extract attenuated $H_2O_2$-induced cell death and reactive oxygen species (ROS) generation in SK-N-SH cells. MWV extract showed significantly higher DPPH radical scavenger activity when compared to normal kimchi extract. MWV extract showed an inhibitory effect on brain neuronal cell death against oxidative stress by antioxidant activities.

Kimchi and Nitrosamines (김치와 니트로소아민)

  • Park, Kun-Young;Cheigh, Hong-Sik
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.21 no.1
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    • pp.109-116
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    • 1992
  • Kimchi is a very popular traditional fermented vegetable in Korea, however, contaminations of $NO_3$, $NO_2$ and nitrosamines in Kimchi were suspected. In this review, ingredients used in manufacturing Kimchi, kinds of Kimchi, microorganisms involved the fermentation and nutritional values of Kimchi are introduced. The quantitated levels of $NO_3$, $NO_2$ and secondary amines in the ingredients of Kimchi, and the changes of the contents of $NO_3$, $NO_2$ and nitrosamines during Kimchi fermentation were shown. The level of $NO_3$ during Kimchi fermentation reduced greatly, and the content of $NO_2$ was also trace amounts. NDMA is a major nitrosamine found in Kimchi, but the levels were trace or not detected.

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The Influence of Some Soil-treated Herbicides on the Mineralization of Nitrogen Fertilizers II. In an upland soil (토양처리형 제초제가 질소비료의 무기화작용에 미치는 영향 II 밭 토양 조건)

  • Kim Moo Key
    • Korean journal of applied entomology
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    • v.16 no.3 s.32
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    • pp.149-154
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    • 1977
  • Effect of Simazine(2-chloro-4,6-bis (ethylamino)-s-triazine), Nitrofen (2, 4-dichloro-4' -nitrodi­phenylether), Propanil (:3, 4-dichloropropionanilide), and Butachlor (2-chloro-2, 6-diethyl N-(buthoxy­menthyl) acetanilide on urea hydrolysis and subsequent nitrification was investigated in an upland soil incubated at $20\pm1^{\circ}C$. 1. All the herbicides tested had no effect on the hydrolysis of urea to ammonia at the recommended rates. Butachlor, at ten and fifty times the recommended rate, and Nitrofen, at fifty times the recommeded rate, depressed urea hydrolysis, resulting in reduction of ammonia. But the depressive effects were temporary, disappearing soon. Simazine and Propanil had no detrimental effect on urea decomposition at all the treated rates. 2. Also, all the chemicals tested had no effect on the nitrification process at the recommended rates. At higher concentrations of ten and fifty times the recommended rate Butachlor and Nitrofen inhibited the oxidation of nitrite, and propanil long inhibited the oxidation of ammonium to nitrite, but was inactive against nitrite oxidizer. These inhibitive effects of the chemicals, however, disappeared in the later period of incubation. Simazine had no effect on the nitrification process at all the treated rates. 3. The trend of change in soil pH of both the treated and untreated plots well reflected the change of soil nitrogen forms during incubation. No direct effect of the chemicals on soil pH was obserbed.

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Comparative analysis of nitrite scavenging activity and anti-inflammation effects in the fruiting bodies of medicinal mushrooms (약용버섯류 자실체의 아질산염 소거능 및 항염증 효능 분석)

  • Cho, Jae-Han;Lee, Gang-Hyo;Han, Jae-Gu;Kim, Hyung-Don;Jhune, Chang-Sung
    • Journal of Mushroom
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    • v.13 no.4
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    • pp.330-333
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    • 2015
  • This study was carried out to compare the anti-inflammation effects of various fruiting body of Ganoderma species and Cordyceps militaris, Phelinus linteus extracts. We concentrated Ganoderma species and other medicinal mushrooms by extracting with ethanol. And We made it $100{\mu}g/ml$ concentration. As a result of nitrite scavenging activity, in the contrast to the positive control; Ascorbic acid was 25%, ASI 7080 of Ganoderma species was disappeared up to around 40%. And in the contrast to Ascorbic acid was 55%, ASI 7002 was 78.5% that was the highest anti-inflammation effect in the result of "No assay test". The Cordyceps militaris showed 75% and Hericium erinaceus showed 59.7% of anti-inflammation effect. As a result of the fungus yield control test of $TNF-{\alpha}$ through ELISA method to ASI 7002 of Ganoderma species that showed the highest anti-inflammation, it was reduced as same as LPS non-treatment. We extracted RNA from ASI 7002 Ganoderma species 10, 50, $100{\mu}g/ml$ concentration and LPS $10{\mu}g/ml$ of Raw 264.7 cell. And we tested the expression of iNOS, COX-2 and TNF-a that are kinds of inflammation gene after synthesizing RNA with cDNA. Finally we could find that iNOS, COX-2 and TNF-a were all controlled expression in the result of above experiment.

Effect of Substituted Conjugated Linoleic Acid for Fat on Meat Qualities, Lipid Oxidation and Residual Nitrite Content in Emulsion-type Sausage

  • Hah, K.H.;Yang, H.S.;Hur, S.J.;Moon, S.S.;Ha, Y.L.;Park, G.B.;Joo, Seon-Tea
    • Asian-Australasian Journal of Animal Sciences
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    • v.19 no.5
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    • pp.744-750
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    • 2006
  • An experiment was carried out to evaluate the effects of substituted conjugated linoleic acid (CLA) for fat on qualities, lipid oxidation and residual nitrite contents in emulsion-type sausage (ETS) after different periods of storage. The control sausage contained 20% fat, however, the substituted CLA for fat sausages contained 17.5% fat+2.5% CLA, 15% fat+5% CLA, 12.5% fat+7.5% CLA and 10% fat+10% CLA, respectively. CLA concentration was significantly increased (p<0.05) by substitution of CLA sources for fat, and storage did not affect the CLA concentration. TBARS were significantly increased with the increasing of storage time in all treatments (p<0.05). Significantly higher $L^*$ values were observed in the 10% CLA treatment compared to other treatments during storage. Moreover, $a^*$ values in CLA treatments significantly (p<0.05) increased compared to the control. Again, $b^*$ values were significantly (p<0.05) higher in CLA treatments than those of the control during storage. The concentration of residual nitrite in ETS was significantly (p<0.05) different between the treatments during storage and the results were in the following order: control >2.5% CLA>5% CLA and 7.5% CLA>10% CLA. Overall acceptability of CLA treatments was significantly (p<0.05) lower than the control during storage, whereas no significant differences were found in overall acceptability between the treatments after 28 days of storage.

Correlationship of Vertical Distribution for Ammonia Ion, Nitrate Ion and Nitrifying Bacteria in a Fixed Bed Nitrifying Biofilm

  • Choi, Gi-Chung;Byun, Im-Gyu
    • Journal of Environmental Science International
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    • v.21 no.12
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    • pp.1455-1462
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    • 2012
  • The vertical distributions of nitrifying bacteria in aerobic fixed biofilm were investigated to evaluate the relationship between nitrification performance and microbial community at different HRT. Fluorescent in situ hybridization (FISH) and portable ion selective microelectrode system were adopted to analyze microbial communities and ions profiles according to the biofilm depth. Cilia media packed MLE (Modified Ludzack-Ettinger) like reactor composed of anoxic, aerobic I/II was operated with synthetic wastewater having COD 200 mg/L and $NH_4{^+}$-N mg/L at HRT of 6 hrs and 4 hrs. Total biofilm thickness of aerobic I, II reactor at 4 hrs condition was over two times than that of 6 hrs condition due to the sufficient substrate supply at 4 hrs condition (6 hrs; aerobic I 380 ${\mu}m$ and II 400 ${\mu}m$, 4 hrs; aerobic I 830 ${\mu}m$ and II 1040 ${\mu}m$). As deepen the biofilm detection point, the ratio of ammonia oxidizing bacteria (AOB) was decreased while the ratio of nitrite oxidizing bacteria (NOB) was maintained similar distribution at both HRT condition. The ratio of AOB was higher at 4 hrs than 6 hrs condition and $NH_4{^+}$-N removal efficiency was also higher at 4 hrs with 89.2% than 65.4% of 6 hrs. However, the ratio of NOB was decreased when HRT was reduced from 6 hrs to 4 hrs and $NO_2{^-}$-N accumulation was observed at 4 hrs condition. Therefore, it is considered that insufficient HRT condition could supply sufficient substrate and enrichment of AOB in all depth of fixed biofilm but cause decrease of NOB and nitrite accumulation.