• Title/Summary/Keyword: Nitrates

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Isolation and Identification of Pathogenic Microorganisms from Soybean Sprouts

  • Kim, Hye-Jung;Koo, Kyoung-Mo;Kim, Gi-Nahm;Lee, Dong-Sun;Paik, Hyun-Dong
    • Preventive Nutrition and Food Science
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    • v.7 no.3
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    • pp.305-309
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    • 2002
  • Raw soybean sprouts were tested for contamination with the following bacteria which have potential for pathogenesis or food spoilage : Salmonella spp., Escherichia coli O157:H7, Yersinia enterocolitica, Vibrio parahae-molyticus, Aeromonas hydrophila, Plesidomonas shigeloides, Pseudomonas aeruginosa, Staphylococcus aureus, Lis-teria monocytogenes, Bacillus cereus, Clostridium perfringens, Campylobacter jejuni, Erwinia spp., and Fusarium spp. Three of the above strains were isolated from the sprouts, and identified by morphological and biochemical methods including an API kit and ATB automated identification system. The isolate cultured in Cereus selective agar, a selective medium, was a Gram-positive, rod shaped, anaerobic spore former. The biochemical and culture tests revealed the following characteristics: catalase-positive, no growth on Simmon's citrate, NO₂ production and requirement of arginine for growth; the ATB automated identification system gave 99.8 % agreement for the identification of Bacillus cereus to the species level. The isolate cultured in Macconkey agar selective medium was Gram-negative, rod shaped and a gas former; the ATB-system gave 99.9% agreement for the identification of Aeromonas hydrophila to the species level. The isolate found in Pseudomonas isolation agar was Gram-negative, rod shaped, cytochrome oxidase-positive, a reducer of nitrates to nitrogen, and pyocyanin producer; the ATB-system gave 99.9 % agreement for the identification of Pseudomonas aeruginosa to the species level. These results indicate that the three bacteria species present in the soybean sprouts were Bacillus cereus, Aero-monas hydrophila, and Pseudomonas aeruginosa. Salmonella spp., Escherichia coli O157:H7, and Yersinia enter-ocolitica, which are associated with serious disease in humans, were not isolated from soybean sprouts examined in this study.

Influence of Carbon and Nitrogen Sources in Solubilization of Hardly Soluble Mineral Phosphates by Penicillium Oxalicum CBPS-Tsa

  • Kim, Eun-Hee;Sundaram, Seshadri;Park, Myoung-Su;Shin, Wan-Sik;Sa, Tong-Min
    • Korean Journal of Environmental Agriculture
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    • v.22 no.3
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    • pp.197-202
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    • 2003
  • Phosphorus is one of the major plant growth limiting nutrients, despite being abundant in soils in both inorganic and organic forms. Phosphobioinoculants in the form of microorganisms can help in increasing the availability of accumulated phosphates for plant growth by solubilization. Penicillium oxalicum CBPSTsa, isolated from paddy rhizosphere, was studied for its phosphate solubilization. The influence of various carbon sources like glucose, sucrose, mannitol and sorbitol and nitrogen sources like arginine, sodium nitrate, potassium nitrate, ammonium chloride and ammonium sulphate were evaluated using liquid media with tricalcium phosphate (Ca-P), ferric phosphate (Fe-P) and aluminium phosphate (Al-P). Maximum soluble phosphate of 824 mg/L was found in the amendment of sucrose-sodium nitrate from 5 g/L of Ca-P. Mannitol, sorbitol, and ariginine were poor in phosphate solubilization. While sucrose was better carbon source in solubilization of Ca-P and Al-P, glucose fared better in solubilization of Fe-P. Though all the nitrogen sources enhanced P solubilization, nitrates were better than ammonium In the amendments of ammonium chloride and ammonium sulphate, higher uptake of available phosphates by the fungus was found, and this resulted in depletion of available P in Fe-P amendment Phosphate solubilization was accompanied by acidification of the media, and the highest pH decrease was observed in glucose amendment Among the nitrogen sources, ammonium chloride favored greater pH decrease.

Synthesis and Characterization of Y2O3 Powders by a Modified Solvothermal Process

  • Jeong, Kwang-Jin;Bae, Dong-Sik
    • Korean Journal of Materials Research
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    • v.22 no.2
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    • pp.78-81
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    • 2012
  • $Y_2O_3$ nanomaterials have been widely used in transparent ceramics and luminescent devices. Recently, many studies have focused on controlling the size and morphology of $Y_2O_3$ in order to obtain better material performance. $Y_2O_3$ powders were prepared under a modified solvothermal condition involving precipitation from metal nitrates with aqueous ammonium hydroxide. The powders were obtained at temperatures at $250^{\circ}C$ after a 6h process. The properties of the $Y_2O_3$ powders were studied as a function of the solvent ratio. The synthesis of $Y_2O_3$ crystalline particles is possible under a modified solvothermal condition in a water/ethylene glycol solution. Solvothermal processing condition parameters including the pH, reaction temperature and solvent ratio, have significant effects on the formation, phase component, morphology and particle size of yttria powders. Ethylene glycol is a versatile, widely used, inexpensive, and safe capping organic molecule for uniform nanoparticles besides as a solvent. The characterization of the synthesized Y2O3 powders were studied by XRD, SEM (FE-SEM) and TG/DSC. An X-ray diffraction analysis of the synthesized powders indicated the formation of the $Y_2O_3$ cubic structure upon calcination. The average crystalline sizes and distribution of the synthesized $Y_2O_3$ powders was less than 2 um and broad, respectively. The synthesized particles were spherical and hexagonal in shape. The morphology of the synthesized powders changed with the water and ethylene glycol ratio. The average size and shape of the synthesized particles could be controlled by adjusting the solvent ratio.

X-ray absorption spectroscopic study of MgFe2O4 nanoparticles

  • Singh, Jitendra Pal;Lim, Weon Cheol;Song, Jonghan;Kim, Joon Kon;Chae, Keun Hwa
    • Proceedings of the Korean Vacuum Society Conference
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    • 2015.08a
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    • pp.230.2-230.2
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    • 2015
  • Nanoparticles of magnesium ferrite are used as a heterogeneous catalyst, humidity sensor, oxygen sensor and cure of local hyperthermia. These applications usually utilize the magnetic behavior of these nanoparticles. Moreover, magnetic properties of nanoferrites exhibit rather complex behavior compared to bulk ferrite. The magnetic properties of ferrites are complicated by spins at vortices, surface spins. Reports till date indicate strong dependency on the structural parameters, oxidation state of metal ions and their presence in octahedral and tetrahedral environment. Thus we have carried out investigation on magnesium ferrite nanoparticles in order to study coordination, oxidation state and structural distortion. For present work, magnesium ferrite nanoparticles were synthesized using nitrates of metal ions and citric acid. Fe L-edge spectra measured for these nanoparticles shows attributes of $Fe^{3+}$ in high spin state. Moreover O K-edge spectra for these nanoparticles exhibit spectral features that arises due to unoccupied states of O 2p character hybridized with metal ions. Mg K-edge spectra shows spectral features at 1304, 1307, 1311 and 1324 eV for nanoparticles obtained after annealing at 400, 500, 600, 800, 1000, and $1200^{\circ}C$. Apart from this, spectra for precursor and nanoparticles obtained at $300^{\circ}C$ exhibit a broad peak centered around 1305 eV. A shoulde rlike structure is present at 1301 eV in spectra for precursor. This feature does not appear after annealing. After annealing a small kink appear at ~1297 eV in Mg K-edge spectra for all nanoparticles. This indicates changes in local electronic structure during annealing of precursor. Observed behavior of change in local electronic structure will be discussed on the basis of existing theories.

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The Content of N-nitrosamine in Mollusk Crustacea and Shellfish (연체류, 갑각류 및 패류 중 N-Nitrosamine 함량)

  • Oh, Myung-Cheol;Oh, Chang-Kyung;Yang, Tai-Suk;Kim, Bong-Oh;Kim, Soo-Hyun;Oh, Hyuk-Soo
    • Culinary science and hospitality research
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    • v.11 no.4 s.27
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    • pp.1-13
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    • 2005
  • This study analyzed the levels of N-nitrosamine(NA) and its precursors such as nitrite, nitrate, dimethylamine and trimethylamine in 10 samples of mollusk fish, 4 samples of crustacea fish and 11 samples of shellfish from fish distributed in a local markets. Mollusk fishes had nitrite concentrations ranging from non-detectable(ND) to 9.4 mg/kg, crustacea fishes ND to 8.8 mg/kg, and shellfishes ND to 4.3 mg/kg. Nitrates in mollusk fishes ranged from ND to 19.3 mg/kg, crustacea fishes 4.1 to 79.9 mg/kg, and shellfishes 1.5 to 61.9 mg/kg. DMK concentrations were 11.2 to 551.4 mg/100g in mollusk fishes, 44.4 to 79.9 mg/100g in crustacea fishes, and 1.3 to 5.9 mg/100g in shellfishes. TMA concentrations in mollusk fishes, crustacea fishes, and shellfishes were 10.3${\sim}$292.4 mg/100g, 35.5${\sim}$90.3 mg/100g, and 2.3${\sim}$17.1 mg/100g respectively. Only N-nitrosodimethylamine (NDMA) was detected for NA in fish distributed in local markets. NDMA contentrations ranged from ND to 41.4 ${\mu}g/kg$ in mollusk fishes, 3.0 to 47.3 ${\mu}g/kg$ in crustacea fishes, and 1.7 to 12.1 ${\mu}g/kg$ in shellfishes.

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Synthesis and characterization of the ultrafine $ZnFe_2O_4$ powder by glycothermal (Glycothermal에 의한 초미립의 $ZnFe_2O_4$분말 합성 및 특성)

  • Bae, Dong-Sik;Han, Kyong-Sop;Cho, Seung-Beom;Choi, Sang-Hael
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.7 no.1
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    • pp.167-173
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    • 1997
  • The $ZnFe_2O_4$ powder was prepared under glycothermal conditions by precipitation from metal nitrates with aqueous potassium hydroxide. The fine powder was obtained at temperatures as low as 225 to $300^{\circ}C$. The microstructure and phase of the $ZnFe_2O_4$ powder were studied by SEM and XRD. The properties of the powder were studied as a function of various parameters (reaction temperature, reaction time, solid loading, etc). The average particle size of the $ZnFe_2O_4$ increased with increasing reaction temperature. After glycothermal treatment at $270^{\circ}C$ for 8 h, the average particle diameter of the $ZnFe_2O_4$ was about 50 nm.

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Fabrication of the ultrafine ZnO powder through glycothermal process (Glycothermal 공정에 의한 미립 ZnO 분말의 제조)

  • Dong-Sik Bae;Kyong-Sop Han;Yong-Kap Park;Seung-Beom Cho;Sang -Heul Choi
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.7 no.4
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    • pp.567-572
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    • 1997
  • The ZnO powder was prepared under glycothermal conditions by precipitation from metal nitrates with aqueous potassium hydroxide. The fine powder was obtained at temperatures as low as 225 to $275^{\circ}C$, The microstructure and phase of the powder were studied by SEM and XRD. The properties of the ZnO powder were studied as a function of various parameters (reaction temperature, reaction time, solid loading, etc). The average particle size of the ZnO increased with increasing reaction temperature. After glycothermal treatment at $225^{\circ}C$ for 8 h, the average particle size of the ZnO powder was about 150 nm and the particle size distribution was narrow.

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The Effect of Promotor and Reaction Condition for FT Oil Synthesis over 12wt% Co-based Catalyst (12wt% Co 담지 촉매에서 합성오일 제조시 조촉매 효과 및 반응조건 영향 분석)

  • Park, Yonhee;Lee, Jiyoon;Jung, Jongtae;Lee, Jongyeol;Cho, Wonjun;Baek, Youngsoon
    • Transactions of the Korean hydrogen and new energy society
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    • v.25 no.3
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    • pp.247-254
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    • 2014
  • The synthesis of Fischer-Tropsch oil is the catalytic hydrogenation of CO to give a range of products, which can be used for the production of high-quality diesel fuel, gasoline and linear chemicals. Our cobalt based catalyst was prepared Co/alumina, silica and titania by the incipient wet impregnation of the nitrates of cobalt and promoter with supports. Cobalt catalysts was calcined at $350^{\circ}C$ before being loaded into the FT reactors. After the reduction of catalyst has been carried out under $450^{\circ}C$ for 24hrs, FT reaction of the catalyst has been carried out at GHSV of 4,000/hr under $200^{\circ}C$ and 20atm. From these test results, we have obtained the results as following ; in case of 12wt% Co-supported $Al_2O_3$, $SiO_2$ and $TiO_2$ catalysts, maximum activities of the catalysts were appeared at the promoters of Mn, Mo and Ce respectively. The activity of 12wt% $Co/Al_2O_3$ added a Mn promoter was about 3 times as high as that of 12wt% $Co/Al_2O_3$ catalyst without promoters. When it has been the experiment at the range of reaction temperature of $200{\sim}220^{\circ}C$ and GHSV of 1,546~5,000/hr, the results have shown generally increasing the activities with the increase of reaction temperature and GHSV.

Correlation of Releases of Nutrient Salts in Sediment with Vicinal Oxic Conditions (퇴적물의 영양염류 용출과 호기적 조건과의 상관성 분석)

  • Cho, Dae-Chul;Lee, Eun-Mi;Park, Byung-Gi;Kwon, Sung-Hyun
    • Journal of Environmental Science International
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    • v.20 no.7
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    • pp.845-855
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    • 2011
  • The aim of this paper is to correlate the release characteristics of marine and lake sediment with their vicinal oxic conditions. We performed lab-scale simulation experiments using field sediment and water in order to compare the release concentrations and the release rates one another. To provide a few different kinds of oxic environments we used natural air flow and some oxygen releasing compounds such as $CaO_2$ and $MgO_2$. In case of phosphates, in each oxic condition, removal of phosphorus via biological activity and that via salt precipitation with the metal ions lowered the release rates. The behavior of the nitrogen-origin salts seemed to greatly depend on the typical biological actions - growth of biomass, nitrification, and partial denitrification. Generally speaking, the control of releases of $NH_3$-N, $PO_4$-P, T-N and T-P was successful under the oxic conditions meanwhile COD, nitrates and nitrites were difficult to reduce the releases into the bulk water because of the considerable microbial oxidation. Based on typical diffusive mass transfer kinetics the changes of concentrations of the nutrients were computed for qualitative and quantitative comparisons.

Status of Water Quality and Future Plans in the Philippines (필리핀의 수질현황 및 미래 관리계획)

  • Gorme, Joan B.;Maniquiz, Marla C.;Kim, Lee-Hyung
    • Journal of Wetlands Research
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    • v.11 no.3
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    • pp.89-103
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    • 2009
  • The Philippines is abundant with rich natural resources such as water. Because of rapid urbanization in the country, most of the water bodies are polluted as a result of domestic, agricultural and industrial activities. The Environmental Management Bureau (EMB) is the main government arm responsible for monitoring and inspection of affected water bodies. Only water bodies with at least four sampling events during dry and wet seasons were included in the assessment of water bodies that passed the DAO 90-34 water quality criteria. Water bodies were monitored for dissolved oxygen (DO), biochemical oxygen demand (BOD), total suspended solids (TSS), and total dissolved solids (TDS), heavy metals, fecal coliform contamination and nitrates. High pollutant concentrations from domestic, agricultural, industrial and nonpoint sources were observed from monitoring events due to inadequate sewage services and treatment facilities in the country. The objective of this paper was to present and evaluate the quality of the water bodies in the Philippines.

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