• The Journal of the Korea Contents Association
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• v.17 no.1
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• pp.92-99
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• 2017

Kicking someone has clothes on causes direct contact between victim's clothes and skin. This contact makes material exchange from skin to inner clothes. In this study, foot impressions by transferred to cloths from skin would be enhanced by amino acid staining reagent (ninhydrin, 1,2-IND/Zn, DFO). In view of research ethics, we conducted this research with porcine skin instead of human's one. To mimic human's skin condition, applied artificial sweat on porcine skin. According to results, ninhydrin showed high contrast on light colored background and 1,2-IND/Zn, DFO showed high contrast on dark colored background. It showed different results up to kind of fabrics.

• Analytical Science and Technology
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• v.28 no.1
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• pp.26-32
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• 2015

The consistency and homogeneity of repetitive printing of artificial fingerprint were evaluated using a visual minutiae comparison method and an Automated Fingerprint Identification System (AFIS). The standard latent fingerprint pattern was prepared by the printing of a master digital fingerprint pattern with an inkjet printer cartridge case filled with artificial sweat. The master digital fingerprint pattern was prepared with a scanning of an inked fingerprint pattern of a living subject. The intensities of the master digital fingerprint pattern were adjusted by changing the 'output level' values of the Adobe Photoshop CS 5 software. Number of standard latent fingerprint patterns were printed and then developed with conventional latent fingerprint developing methods; ninhydrin treatment method and 1,2-indandion(1,2-IND)/$ZnCl_2$ treatment method. The ridge details of the latent fingerprint patterns developed with the reagents were visually compared with the inked fingerprint pattern and could confirm that the minutiae of both patterns are visually identical. The ridge detail of the inked fingerprint and reagent developed standard latent fingerprint patterns were compared with an AFIS. The average number of minutiae searched by the AFIS was $52.4{\pm}2.4$ (range = 48~56) for 50 ninhydrin developed latent fingerprint patterns, and $50.2{\pm}1.9$ (range = 47~53) for 50 1,2-IND/$ZnCl_2$ developed latent fingerprint patterns. These low standard deviation values over 50 repetitive printing demonstrated that the 50 standard latent patterns were printed with consistent and homogeneous manner.

• Bulletin of the Korean Chemical Society
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• v.20 no.10
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• pp.1229-1231
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• 1999

• Journal of Applied Biological Chemistry
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• v.50 no.4
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• pp.221-226
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• 2007

The high molecular-weight mucilage extracted and purified from cactus fruit of Opuntia ficus-indica var. Saboten was degraded by the cell-free culture filtrate of a fungus isolated from soil. TLC analysis of the polymeric mucilage after incubation with the fungal culture filtrate confirmed its degradation. When the degradation products were tested for their qualitative reactions with ninhydrin and phenol-sulfuric acid, only phenol-sulfuric acid gave positive development, and ninhydrin did not show any observable color reaction. This coloring reaction suggested the presence of a carbohydrate without an amino group within the mucilage. Analyses by HPLC and liquid gel permeation chromatography on sephadex G-100 also provided additional information on degradation of the mucilage by the fungal culture filtrate. The sequences of ITS-5.8S rDNA from the fungal isolate that was cultivated for the preparation of mucilage-degrading enzyme showed 99% similarity to those of Pestalotiopsis aquatica.

• Journal of the Korean Society of Tobacco Science
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• v.32 no.2
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• pp.70-76
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• 2010

A simple and sensitive automatic amino acid analyzer method for the determination of free amino acids in tobacco was described. Sample preparation consisted of a single step of extraction with 0.1 mol HCl at ambient temperature in 60 min by sonication, followed by filtration of an aliquot. Automated amino acid analyzer was used to construct a post-column ninhydrin reaction unit to monitor amino acids separated by liquid chromatography using a series of eluting buffers. By optimization of sample preparation, separation of 19 amino acids was achieved. Limits of quantitation was 0.01 mg/g, coefficients of variation ranged from 0.5 % to 8.9 % and recoveries range from 85 % to 106 %. The method was applied to the analysis of amino acids contents of tobacco leaves in different varieties.

• Bulletin of the Korean Chemical Society
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• v.23 no.11
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• pp.1651-1654
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• 2002

• Fire Science and Engineering
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• v.32 no.3
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• pp.88-94
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• 2018

Fingerprints deposited on paper found at a fire scene are important evidence for determining a suspect's identity. On the other hand, because the temperature at the fire scene is very high, there are many difficulties in developing fingerprints. This study compared the effectiveness of ninhydrin, DFO, and silver nitrate to develop fingerprints deposited on paper exposed to high temperatures. Using a dry heat press, heat was applied to the paper at $200^{\circ}C$ for various times. Silver nitrate was found to be superior to the others under all conditions. Ninhydrin developed fingerprints weakly compared to DFO and silver nitrate. In addition, the sensitivity of DFO decreased with increasing exposure time. Therefore, it is recommended silver nitrate be used to develop fingerprints deposited on paper found at a fire scene.

• The Korean Journal of Food And Nutrition
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• v.3 no.2
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• pp.207-210
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• 1990

Paper chromatography and (NH4)2SO4 precipitation method were used for refinement and separation of proteins Color reaction was tested by Biuret test, Million test, Xanthoprotein test, and Ninhydrin test. Precipitation reaction and coagulation of proteins were tested by heating method (Ethanol, HNO3).

• Bulletin of the Korean Chemical Society
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• v.30 no.8
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• pp.1877-1880
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• 2009

• Microbiology and Biotechnology Letters
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• v.19 no.4
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• pp.372-379
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• 1991

A rapid and simple quantitative assay method for aminoglycoside-3'- phosphotransferase (APH(3')) derived from E. coli ATCC 21990 was developed using the thin layer chromatographic densitometry, 3'-phosphorylated kanamycin B (3'-PKMB), product of APH (3') reaction, was separated from reaction mixtures by developing on the silica gel TLC plate with chloroform-methanol-ammonia water (3:4:3). The quantity of the 3'-PKMB was measured by densitometry after color development by ninhydrin method. Densitometric TLC assay for APH (3') was showed a good quantitative result and reproducibility. Sensitivity of this assay was 1.56 nmol of 3'-PKMB and could be analyzed many samples at same time. This method may be applicable for the analysis of inactivating enzymes of aminoglycoside antibiotics.