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Molecular Characterization of an Isolate of Bean Common Mosaic Virus First Identified in Gardenia Using Metatranscriptome and Small RNA Sequencing

  • Zhong-Tian Xu;Hai-Tao Weng;Jian-Ping Chen;Chuan-Xi Zhang;Jun-Min Li;Yi-Yuan Li
    • The Plant Pathology Journal
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    • v.40 no.1
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    • pp.73-82
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    • 2024
  • Gardenia (Gardenia jasminoides) is a popular and economically vital plant known for its ornamental and medicinal properties. Despite its widespread cultivation, there has been no documentation of plant viruses on gardenia yet. In the present study, gardenia leaves exhibiting symptoms of plant viral diseases were sampled and sequenced by both metatranscriptome and small RNA sequencing. As a consequence, bean common mosaic virus (BCMV) was identified in gardenia for the first time and named BCMV-gardenia. The full genome sequence of BCMV-gardenia is 10,054 nucleotides (nt) in length (excluding the poly (A) at the 3' termini), encoding a large polyprotein of 3,222 amino acids. Sequence analysis showed that the N-termini of the polyprotein encoded by BCMV-gardenia is less conserved when compared to other BCMV isolates, whereas the C-termini is the most conserved. Maximum likelihood phylogenetic analysis showed that BCMVgardenia was clustered closely with other BCMV isolates identified outside the leguminous plants. Our results indicated that the majority of BCMV-gardenia virus-derived small interfering RNAs (vsiRNAs) were 21 nt and 22 nt, with 21 nt being more abundant. The first nucleotide at the 5' termini of vsiRNAs derived from BCMV-gardenia preferred U and A. The ratio of vsiRNAs derived from sense (51.1%) and antisense (48.9%) strands is approaching, and the distribution of vsiRNAs along the viral genome is generally even, with some hot spots forming in local regions. Our findings could provide new insights into the diversity, evolution, and host expansion of BCMV and contribute to the prevention and treatment of this virus.

ALGEBRAIC RICCI SOLITONS IN THE FINSLERIAN CASE

  • Jiao, Guocheng;Yan, Zaili
    • Bulletin of the Korean Mathematical Society
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    • v.58 no.4
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    • pp.853-863
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    • 2021
  • In this paper, we study algebraic Ricci solitons in the Finslerian case. We show that any simply connected Finslerian algebraic Ricci soliton is a Finslerian Ricci soliton. Furthermore, we study Randers algebraic Ricci solitons. It turns out that a shrinking, steady, or expanding Randers algebraic Ricci soliton with vanishing S-curvature is Einstein, locally Minkowskian, or Riemannian, respectively.

Cryptotanshinone Induces Inhibition of Breast Tumor Growth by Cytotoxic CD4+ T Cells through the JAK2/STAT4/ Perforin Pathway

  • Zhou, Jun;Xu, Xiao-Zhen;Hu, Yao-Ren;Hu, Ai-Rong;Zhu, Cheng-Liang;Gao, Guo-Sheng
    • Asian Pacific Journal of Cancer Prevention
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    • v.15 no.6
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    • pp.2439-2445
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    • 2014
  • Cryptotanshinone (CPT), is a quinoid diterpene isolated from the root of the Asian medicinal plant, Salvia miotiorrhiza bunge. Numerous researchers have found that it could work as a potent antitumor agent to inhibit tumor growth in vitro, buith there has been much less emphasis on its in vivo role against breast tumors. Using a mouse tumor model of MCF7 cells, we showed that CPT strongly inhibited MCF7 cell growth in vivo with polarization of immune reactions toward Th1-type responses, stimulation of naive CD4+ T cell proliferation, and also increased IFN-${\gamma}$ and perforin production of CD4+ T cells in response to tumor-activated splenocytes. Furthermore, data revealed that the cytotoxic activity of CD4+ T cells induced by CPT was markedly abrogated by concanamycin A(CMA), a perforin inhibitor, but not IFN-${\gamma}$ Ab. On the other hand, after depletion of CD4+ T cells or blocked perforin with CMA in a tumor-bearing model, CPT could not effectively suppress tumor growth, but this phenomenon could be reversed by injecting naive CD4+ T cells. Thus, our results suggested that CPT mainly inhibited breast tumor growth through inducing cytotoxic CD4+ T cells to secrete perforin. We further found that CPT enhanced perforin production of CD4+ T cells by up-regulating JAK2 and STAT4 phosphorylation. These findings suggest a novel potential therapeutic role for CPT in tumor therapy, and demonstrate that CPT performs its antitumor functions through cytotoxic CD4+ T cells.

Development of a Prototype of Guidance System for Rice-transplanter

  • Zhang, Fang-Ming;Shin, Beom-Soo;Feng, Xi-Ming;Li, Yuan;Shou, Ru-Jiang
    • Journal of Biosystems Engineering
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    • v.38 no.4
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    • pp.255-263
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    • 2013
  • Purpose: It is not easy to drive a rice-transplanter avoiding underlapped or overlapped transplanting in paddy fields. An automated guidance system for the riding-type rice-transplanter would be necessary to operate the rice-transplanter autonomously or to assist the beginning drivers as a driving aid. Methods: A prototype of guidance system was composed of embedded computers, RTK-GPS, and a power-steering mechanism. Two Kalman filters were adopted to overcome sparse positioning data (1 Hz) from the RTK-GPS. A global Kalman filter estimated the posture of rice-transplanter every one second, and a local Kalman filter calculated the posture from every new estimation of the global Kalman filter with an interval of 200 ms. A PID controller was applied to the row-following mode control. A control method of U-turning mode was developed as well. A stepping motor with a reduction gear set was used to rotate the shaft of steering wheel. Results: Test trials for U-turning and row-following modes were done in a paddy field after some parameters have been tuned at the ground speed range of 0.3 ~ 1.2 m/s. The minimum RMS error of offset was 3.13 cm at the ground speed of 0.3 m/s while the maximum RMS error was 13.01 cm at 1.2 m/s. The offset RMS error tended to increase as the ground speed increased. The target point distance, LT also affected the system performance and PID controller parameters should be adjusted on different ground speeds. Conclusions: A target angle-based PID controller plus stationary steering angle controller made it possible for the rice-transplanter to steer autonomously by following a reference line accurately and even on U-turning mode. However, as condition in paddy fields is very complicated, the system should control the ground speed that prevents it from deviating too much due to ditch and slope.

Lower Body Shapes of Chinese Males in Their 20s (Report 1) - Centered on the Ningbo Area of Zhejiang Province - (중국(中國) 20대(代) 남성(男性)의 하반신(下半身) 형태(形態) 연구(硏究)(제1보)(第1報) - 절강성 영파 지역(浙江省 寧波 地域)을 중심(中心)으로 -)

  • Lee, So-Young;Shim, Boo-Ja
    • Journal of Fashion Business
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    • v.10 no.5
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    • pp.105-121
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    • 2006
  • Body measurement was first made with the subjects of 193 males in their 20s residing in the Ningbo area, Zhejiang Province in China. In this first report, lower body shapes were classified and discriminated by using anthropometric measurement indices and lower body analysis. The following sums up the research: 1. Compared with the average values of Korean males in their 20s, the Chinese subjects were characterized with thinner waist, more flat hip, and smaller midthigh. 2. The subjects of Chinese males in their 20s showed three types of lower bodies: H-Round Type (25.91%)-thick waist-abdomen, round cross-section waist-abdomen, and small waist-hip difference. A-Trapezoid Type (34.72%)-small width of omphalion waist-abdomen, average cross-section waist-abdomen, and large waist-hip difference. A-Balance Type (39.38%)-average width of omphalion waist-abdomen, flat cross-section waist-abdomen, and large waist-hip difference. 3. Seven useful variables for the categorization of the subjects' lower body types were chosen through stepwise discriminant analysis, and the hit ratio of discrimination was 96.89%.

Body Types of Adult Males in the Ningbo Area of Zhejiang Province in China (중국(中國) 절강성(浙江省) 영파지역(寧波地域) 남성(男性)의 체형연구(體形硏究))

  • Shim, Boo-Ja
    • Journal of Fashion Business
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    • v.11 no.1
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    • pp.108-124
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    • 2007
  • Body measurement was held for the subjects of Chinese adult males in their 20s through 40s residing in the Ningbo area of Zhejiang Province in order to provide body type information and raise the fit of clothing products for the advancement into the Chinese market. The following sums up the analysis of body types based on body measurement: 1. Seven factors to compose body types were produced from the analysis of males in their 20s, explaining 76.07% of variables and representing 3 types according to cluster analysis. Type 1 was H-b and appeared as much as 32.14%. Type 2, semi X-d, was 40.81%, while Type 3, A-i, had 27.04%. 2. Eight body type composing factors were extracted from the analysis of men in their 30s and 40s. The factors explained 76.77% of all the variables and represented 4 types according to cluster analysis. Type 1, H-d, had the appearance rate of 18.47%; Type 2, H-b, 40.84%; Type 3, Y-i, 27.71%; and Type 4, semi X-s, 11.95%.

Molecular Phylogeny and Modular Structure of Hybrid NRPS/PKS Gene Fragment of Pseudoalteromonas sp. NJ6-3-2 Isolated From Marine Sponge Hymeniacidon perleve

  • Zhu, Peng;Zheng, Yanling;You, Yurong;Yan, Xiaojun;Shao, Jianzhong
    • Journal of Microbiology and Biotechnology
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    • v.19 no.3
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    • pp.229-237
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    • 2009
  • Among 12 marine bacterial strains from the China coast that exhibited interesting bioactivity (positive for both antimicrobial and cytotoxic activities), only four strains, namely, NJ6-3-1, NJ6-3-2, NB-6, and YTHM-17, had a KS domain or A domain when screened for PKS and NRPS genes using a PCR. Interestingly, two of these strains belonging to Pseudoalteromonas and associated with the marine sponge Hymeniacidon perleve were positive for both PKS and NRPS, whereas the other two strains of Pseudoalteromonas did not have a PKS or NRPS gene. A molecular phylogeny analysis and DGGE analysis of the Pseudoalteromonas sp. indicated that they had a specific affinity with the host marine sponge Hymeniacidon perleve. Furthermore, an analysis of a partial sequence of Pseudoalteromonas sp. NJ6-3-2 isolated from the marine sponge Hymeniacidon perleve obtained from genomic walking using a computational approach indicated a relatively complete PKS module including auxiliary domains (DH, KR, and Cy).

Large Glass-forming Ability and Magnetocaloric Effect in Gd55Co20Al23Si2 Bulk Metallic Glass

  • Li, Qian;Cai, Pingping;Shen, Baolong;Akihiro, Makino;Akihisa, Inoue
    • Journal of Magnetics
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    • v.16 no.4
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    • pp.440-443
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    • 2011
  • In this study, we investigated the glass-forming ability (GFA) and magnetocaloric effect (MCE) of $Gd_{55}Co_{20}Al_{23}Si_2$ bulk glassy alloy. It is found that the addition of 2 at% Si is effective for extension of the supercooled liquid region (${\Delta}T_x$), the ${\Delta}T_x$ is 55 K for the $Gd_{55}Co_{20}Al_{25}$ glassy alloy, and increases to 79 K for the $Gd_{55}Co_{20}Al_{23}Si_2$ alloy. As a result, $Gd_{55}Co_{20}Al_{23}Si_2$ glassy alloys with diameters up to 5 mm were successfully synthesized. The alloys also exhibit large MCE, i.e., the magnetic entropy change (${\Delta}S_m$) of 8.9 J $kg^{-1}\;K^{-1}$, the full width at half maximum of the ${\Delta}S_m$ (${\delta}T_{FWHM}$) of 87 K, and the refrigerant capacity (RC) of 774 J $kg^{-1}$.

Residual bearing capacity of steel-concrete composite beams under fatigue loading

  • Wang, Bing;Liu, Xiaoling;Zhuge, Ping
    • Structural Engineering and Mechanics
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    • v.77 no.4
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    • pp.559-569
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    • 2021
  • This study was conducted to investigate the residual bearing capacity of steel-concrete composite beams under high-cycle fatigue loading through experiments and theoretical analysis. Six test beams with stud connectors were designed and fabricated for static, complete fatigue, and partial fatigue tests. The failure modes and the degradation of several mechanical performance indicators of the composite beams under high-cycle fatigue loading were analyzed. A calculation method for the residual bearing capacity of the composite beams after certain quantities of cyclic loading cycles was established by introducing nonlinear fatigue damage models for concrete, steel beam, and shear connectors beginning with the material residual strength attenuation process. The results show that the failure mode of the composite beams under the given fatigue load appears to be primarily affected by the number of cycles. As the number of fatigue loadings increases, the failure mode transforms from mid-span concrete crushing to stud cutting. The bearing capacity of a 3.0-m span composite beam after two million fatigue cycles is degraded by 30.7% due to premature failure of the stud. The calculated values of the residual bearing capacity method of the composite beam established in this paper agree well with the test values, which indicates that the model is feasibly applicable.