• Asian-Australasian Journal of Animal Sciences
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• v.29 no.3
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• pp.359-364
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• 2016

The study was carried out to explore the effects of replacing wheat straw with fungal treated wheat straw as an ingredient of total mixed ration (TMR) on the growth performance and nutrient digestibility in Nili Ravi buffalo male calves. Fungal treated wheat straw was prepared using Arachniotus sp. Four TMRs were formulated where wheat straw was replaced with 0 (TMR1), 33 (TMR2), 67 (TMR3), and 100% (TMR4) fungal treated wheat straw in TMR. All TMRs were iso-caloric and iso-nitrogenous. The experimental TMRs were randomly assigned to four groups of male calves (n = 6) according to completely randomized design and the experiment continued for four months. The calves fed TMR2 exhibited a significant improve in dry matter intake, average daily weight gain, feed conversion ratio and feed economics compared to other groups. The same group also showed higher digestibility of dry matter, crude protein, neutral-, and acid detergent fibers than those fed on other TMRs. It is concluded that TMR with 33% fungal-treated wheat straw replacement has a potential to give an enhanced growth performance and nutrient digestibility in male Nili Ravi buffalo calves.

• Asian-Australasian Journal of Animal Sciences
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• v.19 no.9
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• pp.1234-1239
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• 2006

Murrah and NiliRavi are the important North Indian buffalo breeds occupying the prominent position of being the highest milk producers. These breeds are more or less similar at morphological as well as physiological levels. The technique of RAPD-PCR was applied in the present study to identify a battery of suitable random primers to detect genetic polymorphism, elucidation of the genetic structure and rapid assessment of the differences in the genetic composition of these two breeds. A total of 50 random primers were screened in 24 animals each of Murrah and NiliRavi buffaloes to generate RAPD patterns. Of these, 26 (52%) primers amplified the buffalo genome generating 263 reproducible bands. The number of polymorphic bands for the 26 chosen RAPD primers varied from 3 (OPG 06 and B4) to 26 (OPJ 04) with an average of 10.1 bands per primer and size range of 0.2 to 3.2 kb. DNA was also pooled and analyzed to search for population specific markers. Two breed specific RAPD alleles were observed in each of Murrah (OPA02 and OPG16) and NiliRavi (OPG09) DNA pools. RAPD profiles revealed that 11 (4.2%) bands were common to all the 48 individuals of Murrah and NiliRavi buffaloes. Pair-wise band sharing calculated among the individual animals indicated considerable homogeneity of individuals within the breeds. Within breed, band sharing values were relatively greater than those of interbreed values. The low genetic distance (Nei's) value (0.109) estimated in this study is in accordance with the origin and geographical distribution of these breeds. The RAPD analysis indicated high level of genetic similarity between these two important North Indian buffalo breeds.

• Asian-Australasian Journal of Animal Sciences
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• v.13 no.3
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• pp.287-291
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• 2000

The study was based on 1921 service period records of Nili Ravi buffaloes maintained at six dairy farms in Pakistan during the period 1978 to 1994. The mean service period was $237.57{\pm}4.5$ days in the over all data. The highest mean service period ($393.59{\pm}16.18$ days) was at MDF Rawalpindi and the lowest ($199.15{\pm}14.35$ days) was at MDF Peshawar. The difference was significant p<0.001. Late maturing buffaloes showed significantly longer service period as compared to early maturing buffaloes. Parity and seasonal effects on Service Period were studied. There was an overall trend of reduction in the length of service period with the increase in parity. The mean ($287.54{\pm}6.89$ days) service period was (highest) in parity number one and lowest in parity number eight ($107.95{\pm}19.72$ days). The difference was highly significant. In overall data significantly lower service periods were seen in buffaloes calving in spring+winter as compared to summer+fall.

• Asian-Australasian Journal of Animal Sciences
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• v.5 no.2
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• pp.375-382
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• 1992

The effect of certain factors such as sex of calf, status of buffalo, season of calving, parity and sire on lactation length and total lactation yield was studied in 391 Nili-Ravi buffaloes. The average lactation length was $301.73{\pm}1.87$ (mean $\pm$ SE) days with a range of 181 to 505 days whereas the average lactation yield was $2031.08{\pm}19.16kg$ and ranged from 1023 to 6535 kg for 984 lactations. The differences in the means of lactation length and lactation yield due to the sex of calf were significant (p<0.05). The status of buffaloes had a significant (p<0.05) effect on lactation length but its effect on lactation yield was non-significant. The season of calving had no effect on lactation length but it influenced the lactation yield significantly. The milk yield was highest ($2150.81{\pm}43.52kg$) in buffaloes which calved in spring and lowest ($1959.92{\pm}30.83kg$) in autumn. The effect of parity on both traits under study was significant (p<0.01). The maximum and minimum lactation lengths of $309.82{\pm}3.96$ and $284.16{\pm}7.17$ days were observed in the first and sixth lactations, respectively. The milk yield was maximum ($2150.38{\pm}58.79kg$) in the seventh lactation and minimum (1818.31 60.04 kg) in the sixth lactation. The influence of sire was significant on lactation length (p<0.05) and milk yield (p<0.01).

• Asian-Australasian Journal of Animal Sciences
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• v.19 no.11
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• pp.1556-1560
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• 2006

Six breeds of riverine buffalo viz. Murrah, Mehsana, Jaffrabadi, Nagpuri, Nili-Ravi and Bhadawari were characterized using FAO-recommended cattle specific microsatellite markers. Among the total of twenty microsatellite markers screened to explore genomic variability of six buffalo breeds, only ten were polymorphic in nature. Four out of ten polymorphic microsatellite loci were rated as informative. The numbers of alleles detected ranged from 2 to 7, with a mean of $5.5{\pm}0.07$ per microsatellite marker. The most polymorphic marker was BM1818 with a total of 7 alleles present at this locus. One breed specific marker was found in each of Mehsana (BM1818) and Bhadawari (ILSTS030) and four were found in Jaffarabadi (BM1818, ILSTS030, ILSTS054 and ILSTS011). Genetic distance (Ds) between the Mehsana and Bhadawari breed was the maximum (0.29), followed by Murrah and Mehsana (0.27), and Nili-Ravi and Bhadawari (0.26). The lowest Ds was found between the Jaffrabadi and Nagpuri breeds which was only 0.05. The highest divergence time of 1318 years was established between Mehsana and Bhadawari breeds whereas it was found to be lowest (272 years) between the Jaffrabadi and Nagpuri breeds.

• Asian-Australasian Journal of Animal Sciences
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• v.12 no.1
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• pp.56-60
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• 1999

Production and reproduction data of 47 Nili-Ravi buffaloes (162 records) were analyzed with regression techniques. Average lactation milk yield was $2,020.04{\pm}44.59$ liters, lactation length $277.42{\pm}5.70$ d and calving interval $467.10{\pm}11.58$ d. The ranges for these parameters respectively were : 609-3591 lit, 122-614 d and 228-982 d. Year of calving and lactation length had significant effect on total milk yield (p < 0.01), whereas other factors such as month of calving, lactation number and calving interval had no effect on total lactation milk yield. Year of calving had influenced significantly other traits (p < .01) such as calving interval and lactations completed. This indicated considerable environment role in buffalo productivity. Effect of month of calving on total lactation milk yield and other traits was however, found to be non-significant. Nili-Ravi buffaloes produced maximum milk during their first three lactations as compared to subsequent lactations. Regression model explained 40 percent variation in total lactation milk yield due to factors analyzed : animal (dam), year and month of calving lactation length and calving interval.

• Asian-Australasian Journal of Animal Sciences
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• v.12 no.3
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• pp.336-340
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• 1999

Age at maturity was studied in 661 Nili Ravi buffaloes maintained at six dairy farms in Pakistan. The mean age at maturity in the overall data from the six farms was $976.49{\pm}9.2$ days. Significantly lower mean age at maturity $(957.93{\pm}10.68\;days)$ was observed at Military Dairy Farm, Khyber Okara, Military Daiiy Farm, Punjnad and Livestock Research Station, National Agricultural Research Centre, Islamabad (Group I) compared to $(1015.26{\pm}17.39\;days)$ other three Military Dairy Farms, Peshawar, Nowshera and Rawalpindi (Group II). The advantages associated to early age at maturity were as following. Male and female calves were heavier $(38.35{\pm}0.17\;and\;31.84{\pm}15kg,\;respectively)$ in Group I as compared to $(29.27{\pm}0.26\;and\;26.27{\pm}0.26kg)$ in Group II. Milk yield per lactation was significantly higher in Group I $(1912{\pm}12\;lit.)$ as compared to $(1833.36{\pm}16.56\;lit.)$ in Group II. Lactation length was significantly longer $(284.41{\pm}1.23\;days)$ in Group I as compared to $(277.77{\pm}2.02\;days)$ in Group II. Dry period and service period were significantly shorter $(241.59{\pm}4.18\;and\;217.05{\pm}4.95\;days,\;respectively)$ in Group I as compared to $(306.39{\pm}78\;and\;280.95{\pm}9.32\;days)$ in Group II. The mean age at first calving and sex ratio were low ($1282.75{\pm}10.14$ days and 100 ♀ ♀:130.7 ♂ ♂) in Group I as compared to ($1308.7{\pm}16.44$ days and 100 ♀ ♀:152.15 ♂ ♂) in Group II but the differences were non significant.

• Asian-Australasian Journal of Animal Sciences
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• v.10 no.2
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• pp.229-232
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• 1997

Effects of short-term treatment with somidobove (recombinantly produced bovine somatotropin, BST) on estrous cyclicity and fertility were studied in dairy buffaloes. Twenty buffaloes of Nili-Ravi breed calving during the same season were assigned to either control (n=8) or treated group (n=12). The buffaloes of treated group received single infection (prolonged release) of 320 mg of somidobove on day-60 postpartum. The mean values for interval to first postpartum estrus, first service conception rate, services per conception, service period and calving interval for the treated group were 96.4 days, 66.7%, 1.70, 164 days and 473 days, respectively. The corresponding values for the control group were 92.5 days, 62.5%, 1.87, 135 days and 439 days. Means of all variables did not differ between control and treated group (p > 0.05). Three buffaloes of the control and four buffaloes of the treated group did not conceive at first service. Out of these, two buffaloes of control and one buffalo of treated group exhibited normal estrous cycles. It is concluded from these data that short term BST-treatment has no adverse effect on reproductive functions of dairy buffaloes.

• Asian-Australasian Journal of Animal Sciences
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• v.21 no.1
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• pp.51-58
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• 2008

Four ruminally cannulated Nili-ravi buffalo bulls were used in a $4{\times}4$ Latin Square design to determine the influence of varying levels of ruminally degradable protein (RDP) on ruminal characteristics, digestibility, blood pH, blood urea nitrogen (BUN) and nitrogen (N) balance. Four isonitrogenous and isocaloric diets were formulated (NRC, 2001). The control diet contained 50% RDP. The medium (MRDP), high (HRDP) and very high (VHRDP) ruminally degradable protein diets had 66, 82 and 100% RDP, respectively. Increasing the level of dietary RDP resulted in a linear decrease in ruminal pH. A quadratic effect of RDP on ruminal pH was also observed with quadratic maxima at the 66% RDP diet. Dietary RDP had a quadratic effect on total bacterial and protozoal count with maximum microbial count at the 82% RDP diet. Increased microbial count was due to increasing level of ruminal ammonia nitrogen ($NH_3-N$). Increasing dietary RDP resulted in a linear increase in dry matter digestibility. Provision of an adequate amount of RDP caused optimum microbial activity, which resulted in improvement in DM digestibility. Increasing the level of dietary RDP resulted in a linear decrease in crude protein (CP) and neutral detergent fiber digestibility. Blood pH remained unaltered across all diets. A linear increase in ruminal $NH_3-N$ and BUN was noted with increasing level of dietary RDP. The increase in BUN was due to increased ruminal $NH_3-N$ concentrations. A positive N balance was noted across all diets. The results are interpreted to suggest that buffalo bulls can utilize up to 82% RDP of total CP (16%) with optimum results.

• Asian-Australasian Journal of Animal Sciences
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• v.11 no.2
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• pp.128-132
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• 1998

The relative disappearance and rate of degradation of dry matter (DM) and neutral detergent fiber (NDF) of nine different feedstuffs were determined by simultaneously suspending groups of substrates, using the nylon bags, in the rumen of males of Sahiwal cattle and Nili-Ravi buffalo. The digestion kinetics of leguminous forages (Lucerne, berseem and cowpeas) and feed byproducts (cotton seed cake, wheat bran and wheat straw) did not differ between the two species. However, the DM and NDF digestibilities and rates of digestion of grasses and wheat straw were greater in buffalo than in cow bulls, indicating that buffaloes are better converters of poor quality roughages than are Sahiwal. The lag time for DM of grasses did not differ between these two species but the NDF lag time was lower in buffalo than in cows, indicating that both the rate and lag time of digestion may be reliable indicators for assessing the NDF quality.