• The Korean Journal of Mycology
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• v.21 no.2
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• pp.112-119
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• 1993

Various microorganisms secreting plant growth regulators were screened from the 100 microbial isolates including bacteria, actinomycetes and fungi. The isolates showing distict influence on the plant growth were identified as Aspergillus niger. The germinations of Raphanus and Cucubis seeds were completely inhibited by the culture filtrates of A. niger KK, A. niger KKS and A. niger ATCC 9462. The culture filtrates of the three strains also inhibited the formation and development of roots and hypocotyls of Raphanus. The culture filtrates of A. niger ATCC 26550 induced the hypocotyl curvature of Raphanus like plant hormone-auxin and at the same time caused the necrosis of the whole leaves.

• The Journal of Natural Sciences
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• v.5 no.1
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• pp.53-57
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• 1992

This experiment was carried out to elucidate some kinetic properties of the $\alpha$-galactosidase which produced and purified from Aspergillus niger ATCC 16513 and soybean(Glycine max. L). The Km value of Asp. niger and soybean $\alpha$-galactosidase were 37.0mM and 50.0mM for raffinose and55.5mM and 55.5mM for stachyose, respectively. The activity of Asp. niger and soybean $\alpha$-galactosidase were inhibited by galactose. Among the amino acids in active sites of both Asp. niger and soybean $\alpha$-galactosidase, histidine was identified by chemical modification of diethyl pyrocarbonate. Number of amino acids residues per mole of Asp. niger and soybean $\alpha$-galactosidase were 902 and 286, respectively.

• Journal of Korea Soil Environment Society
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• v.1 no.2
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• pp.43-50
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• 1996

The adsorption charateristics of lead(II) ions on Aspergillus niger and Rhizopus arrhizus were investigated. Adsorption amount of A. niger and R. arrhizus was about 95 mg/g and 25 mg/g, respectively. These biomass was approached to adsorption equilibrium within reaction time of 1hr because of their high reactivity. The uptake of lead ion by A. niger was less sensitivity than it by R. arrhizus on the inhibition effect of alkali metals and the decreasing ratio of uptake of lead ion of A. niger and R. arrhizus by inhibition effect of alkali metals was 37% and 50%, respectively. In pre-treatment on these biomass, NaOH treatment was contributed high adsorption capacity to these biomass. Then, adsorption amount of A. niger and R. allhizus was increased about 25 mg/g and 10 mg/g, respectively. In isotherm for the adsorption of lead ion based on Freundlich equation, 1/n value of A. niger and R. ar고izus was calculated the range of 0.28-0.56 and 0.44-0.67, respectively.

• Journal of the Korean Wood Science and Technology
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• v.35 no.5
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• pp.87-92
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• 2007

For the safe disposal of the end used CCA treated wood, the biological method was studied. Aspergillus niger has been known to be grown on the fresh wood surfaces even on the CCA treated wood surface. Therefore, in this study we estimated the removal efficacy of heavy metals from CCA treated wood by A. niger. The selected fungi, A. niger adsorbed the heavy metals from CCA solution. A. niger removed 95% of arsenic, 55% of copper, and 23% of chromium. From these results, A. niger appeared to be useful for the removal of chromium, copper, and arsenic from the end used CCA-treated wood and the use of A. niger-alginate could be effective.

• Journal of the Korean Wood Science and Technology
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• v.33 no.6 s.134
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• pp.101-107
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• 2005

One way of using wood safely and soundly for a long time is use of preservative-treated wood. Although, preservative-treated wood present to us durability and safety during using periods, it is also important to safe disposal after its service periods because of toxic components. Biological methods could be applicable to its disposal methods and better safe than chemical methods in the aspect of environmental problems. This study applied biological methods to remove the heavy metals from end-used CCA treated wood. The Aspergillus niger was used for this study which make black stain on the wood surface by it's spore. This study investigated the growing of A. niger on the CCA treated wood chips and the removal efficiency of heavy metals from CCA treated wood chips using immobilizing A. niger. A. niger could growing on the CCA treated wood chips and A. niger remove the effective elements of CCA treated wood effectively.

• Korean Journal of Agricultural Science
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• v.18 no.1
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• pp.49-73
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• 1991

To elucidate enzymatic properties of $\alpha$-galactosidases (EC3, 2, 1, 22) from germinated soybean and Aspergillus niger changes in the enzyme activities and oligosaccharide contents during germination of soybean were determined and $\alpha$-galactosidases from germinated soybean and wheat bran culture of Aspergillus niger were purified by ammonium sulfate fractionation, ion exchange chromatography and gel filtration. Their chemical and enzymatic properties were investigated and the results obtained were summarized as follows : 1. $\alpha$-Galactosidase activity of soybean was maximized when it was germinated at $25^{\circ}C$ for 120 hours. And raffinose and stachyose in soybean were decomposed completely after 96 hours and 120 hours of germination, respectively. 2. The highest level of $\alpha$-Galactosidase activity was obtained when Aspergillus niger was grown on wheat bran medium at $30^{\circ}C$ for 96 hours. 3. Soybean $\alpha$-galactosidase was purified by 6.6 fold by ammonium slufate fractionation, ion exchange chromatography on DEAE-Cellulose and Sephadex A-50., and gel filtration on Sephadex G-150. Its specific activity was 825 units/mg protein and the yield was 2.5% of the total activity of crude extracts. 4. Aspergillus niger $\alpha$-galactosidase was purified by 23.7 fold. Its specific activity was 1,229 units/mg protein and the yield was 14% of the total activity of wheat bran culture. 5. The purified $\alpha$-galactosidases of soybean and Aspergillus niger were found to be homogeneous by polyacrylamide gel electrophoresis and by HPLC. 6. Chemical properties of the purified $\alpha$-galactosidases were : 1) The soybean $\alpha$-galactosidase was monomeric and its molecular weight was estimated to be 30,000 by SDS-PAGE whereas the Aspergillus niger $\alpha$-galactosidase was a tetrameric glycoprotein which consisted of identical subunits with molecular weight of 28,000 each.

• Applied Biological Chemistry
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• v.44 no.3
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• pp.185-190
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• 2001

Alginate, a well-known biopolymer, is universally applied for immobilization of microbial cells. Biosorption characteristics of lead by waste biomass of immobilized A. niger beads, used in fermentation industries to produce citric acid, were studied. The immobilized A. niger beads, prepared via capillary extrusion method using calcium chloride, were applied in the removal of lead. Pb uptake was the highest in A. niger beads cells grown for 3 days with medium producing citric acid (12% sucrose, 0.5% $NH_4NO_3$, 0.1% $KH_2PO_4$, and 0.025% $MgSO_4$). Lead uptake by the immobilized A. niger beads and free A. niger mycellia beads increased sharply with time. However, while uptake by the immobilized A. niger beads continued to increase slowly, that by free A. niger mycellia beads stopped after 30 min. The optimum pH and temperature of lead uptake were found to be 6 and $35^{\circ}C$, respectively. The maximum uptake of lead was achieved with $50{\sim}100$ beads and 50 ml lead solution in a 250-ml Erlenmeyer flask, while, at over 100 beads, uptake of the lead decreased. The order of biosorption capacity for heavy metals was Pb>Cu>Cd. Pb uptake capacity of the immobilized A. niger beads treated with 0.1 M $CaCI_2$, 0.1 M NaOH, and 0.1 M KOH decreased compared to the untreated beads. On testing the desorption of Pb from the immobilized A. niger beads, re-uptake of Pb was found possible after desorption of the binding metal with 0.1 M HCI.

• Resources Recycling
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• v.22 no.2
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• pp.44-52
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• 2013

Bioleaching behavior of metal ions for recovery of cobalt and copper from cobalt concentrate was investigated by employing three Aspergillus niger strains. Various factors, such as organic acid generation with fungi type, pH of the culture and pulp density were studied. The results showed that the best fungi for organic acid(citric acid and oxalic acid) generation was A. niger KCTC 6144 using Malt Extract Broth culture at initial pH 3.5. But A. niger KCTC 6985 was more effective for the leaching of cobalt and copper from cobalt concentrate. Our results showed that 82% cobalt and 98% copper was dissolved by A. niger KCTC 6985 at 10g/L pulp density, at pH 3.5 and $24^{\circ}C$ after 15 days incubation.

• Resources Recycling
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• v.14 no.5 s.67
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• pp.24-31
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• 2005

In order to recover valuable metals from fine-grained electronic waste, bioleaching of Cu, Zn, Al, Co, Ni, Fe, Sn and Pb were carried out using Aspergillus niger as a leaching microorganism in a shaking flask. Aspergillus niger was able to grow in the presence of electronic scrap. The formation of organic acids(citric and oxalic acid) from Aspergillus niger caused the mobilization of metals from waste electronic scrap. In a preliminary study, in order to obtain the data on the leaching of Cu, Zn, Al, Fe, Co and Ni from electronic scrap, chemical leaching using organic acid(Citric acid and Oxalic acid) was accomplished. At the electronic scrap concentration of 50 g/L, Aspergillus niger were able to leach more than 95% of the available Cu, Co. But Al, Zn, Pb and Sn were leached about 15-35%. Ni and Fe were detected in the leachate less than 10%.

• Journal of the Korean Home Economics Association
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• v.29 no.2
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• pp.47-53
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• 1991

A. niger H-18 and A. fumigatus E-29 wrer selected for their strong abilities to produce cellulase. The λd numerical values of the cotton fabrics inoculated with A. niger H-18 and A. fumigatus E-29 were 580 nm for the both strains of molds. By the growth of molds, lightness, original color scale, and grey scale of the fabrics gradually decreased while chroma increased. The minimum inhibitory concentrations of mold-proofing agents, such as Leperon WL, 8-hydroxyquinoline copper acetate, trimethylol melamine and dimethyl ethylene urea was 50 ppm. Glycoxale was not effective at the above mentioned concentration. Since Leperon WL, trimethylol melamine and dimethyl ethylene urea effectively inhibited the growth of A. niger H-18 and A. fumigatus E-29, tensile strength and elongation of the fabrics were not changed. however, cotton fabrics treated with glycoxale of the fabrics were not changed. However, cotton fabrics treated with glycoxale and inoculated with A. niger H-18 and A. fumigatus E-29 showed decreased in tensile strength by 31.1% and 33.9%, respectively.