• Title/Summary/Keyword: NextGen

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Rapid Screening of Apple mosaic virus in Cultivated Apples by RT-PCR

  • Ryu, Ki-Hyun;Park, Sun-Hee
    • The Plant Pathology Journal
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    • v.19 no.3
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    • pp.159-161
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    • 2003
  • The coat protein (CP) gene of Apple mosaic virus (ApMV), a member of the genus Ilarvirus, was selected for the design of virus-specific primers for amplification and molecular detection of the virus in cultivated apple. A combined assay of reverse transcription and polymerase chain reaction (RT-PCR) was performed with a single pair of ApMV-specific primers and crude nucleic acid extracts from virus-infected apple for rapid detection of the virus. The PCR product was verified by restriction mapping analysis and by sequence determination. The lowest concentration of template viral RNA required for detection was 100 fg. This indicates that the RT-PCR for detection of the virus is a 10$^3$times more sensitive, reproducible and time-saving method than the enzyme-linked immunosorbent assay. The specificity of the primers was verified using other unrelated viral RNAs. No PCR product was observed when Cucumber mosaic virus (Cucumovirus) or a crude extract of healthy apple was used as a template in RT-PCR with the same primers. The PCR product (669 bp) of the CP gene of the virus was cloned into the plasmid vector and result-ant recombinant (pAPCP1) was selected for molecule of apple transformation to breed virus-resistant transgenic apple plants as the next step. This method can be useful for early stage screening of in vitro plantlet and genetic resources of resistant cultivar of apple plants.

Multi-Stage Generation Allocation Game Considering Ramp-rate Constraints (경쟁적 전력시장에서 발전기 증감발률을 고려한 다중시간 발전량 배분 게임)

  • Park, Yong-Gi;Park, Jong-Bae;Roh, Jae-Hyung;Kim, Hyeong-Jung;Shin, Jung-Rin
    • The Transactions of The Korean Institute of Electrical Engineers
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    • v.60 no.3
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    • pp.509-516
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    • 2011
  • This paper studies a novel method to find the profit-maximizing Nash Equilibriums in allocating generation quantities with consideration of ramp-rates under competitive market environment. Each GenCo in a market participates in a game to maximize its profit through competitions and play a game with bidding strategies. In order to find the Nash equilibriums it is necessary to search the feasible combinations of GenCos' strategies which satisfy every participant's profit and no one wants various constraints. During the procedure to find Nash equilibriums, the payoff matrix can be simplified as eliminating the dominated strategies. in each time interval. Because of the ramp-rate, generator's physically or technically limits to increase or decrease outputs in its range, it can restrict the number of bidding strategies of each generator at the next stage. So in this paper, we found the Nash Equilibriums for multi-stage generation allocation game considering the ramp-rate limits of generators. In the case studies, we analyzed the generation allocation game for a 12-hour multi-stage and compared it with the results of dynamic economic dispatch. Both of the two cases were considered generator's ramp-rate effects.

Characterization of the complete mitochondrial genome of Mauritian sardinella, Sardinella jussieu (Lacepède, 1803), collected in the Banten Bay, Indonesia

  • Sektiana, Sinar Pagi;Andriyono, Sapto;Kim, Hyun-Woo
    • Fisheries and Aquatic Sciences
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    • v.20 no.10
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    • pp.26.1-26.9
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    • 2017
  • Fishes in genus Sardinella are small pelagic species, which plays an important role in marine ecosystem as the first consumer. Those species are also commercially important, whose total catch reaches 278,600 tons in 2011 in Indonesia, but their identification has been difficult for their morphological similarity. In this study, we reported Sardinella jussieu for the first time in Indonesian coastal area (Banten Bay, Indonesia, $6^{\circ}\;0^{\prime}\;50.00^{{\prime}{\prime}}\;S-106^{\circ}\;10^{\prime}\;21.00^{{\prime}{\prime}}\;E$). We were able to confirm the species by both its morphological characteristics including the black spot at dorsal fin origin, the dusky pigmentation at caudal fin, 31 total scute numbers, and DNA sequence identity in the GenBank database by the molecular analysis. Its total mitochondrial genome was determined by the combination of next-generation sequencing and typical PCR strategy. The total mitochondrial genome of Sardinella jussieu (16,695 bp) encoded 13 proteins, 2 ribosomal RNAs, 22 transfer RNAs, and the putative control region. All protein-coding genes started with ATG and typical stop codon and ended with TAA or TAG except for ND4 in which AGA is used. Phylogenetic analyses of both COI region and full mitochondrial genome showed that S. jussieu is most closely related to Sardinella albella and Sardinella gibbosa

Trends of Standardization Activity for 5G RAN Architecture (5G RAN 아키텍처 표준동향)

  • Song, P.J.;Song, S.I.
    • Electronics and Telecommunications Trends
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    • v.31 no.6
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    • pp.88-96
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    • 2016
  • 5G RAN은 4G가 진화된 무선기술(evolved E-UTRA)과 5G 무선기술(New Radio, NR)을 모두 지원하는 새로운 무선액세스 네트워크(New RAN)이며, 코어 네트워크 (EPC and NGC)과 접속되어 5G 시스템(NextGen System)을 구성한다. 본고에서는 5G RAN(Radio Access Network,)을 대상으로, RAN 시설 배치 시나리오, RAN과 CN 간 기능 분배 및 인터페이스, 프런트-홀 네트워크의 전송 용량 및 지연, New RAN과 E-UTRA 간 모빌리티 시나리오 등에 대한 표준 이슈를 살펴본다. 마지막 절에선 RAN의 네트워크 슬라이싱 지원 방안으로 상위레벨 개념의 구조와 절차를 정리한다. 본 문서는 3GPP RAN3 그룹의 기술보고서(TR 38.801)와 SA2 기술보고서(TR 23.799)를 바탕으로 작성되었다.

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Challenges; Interoperability of UAV in the NAS (국가공역체계에서 무인항공기 통합운영에 대한 동향)

  • Kim, Joong-Wook;Kim, Do-Hyun
    • Current Industrial and Technological Trends in Aerospace
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    • v.8 no.2
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    • pp.136-144
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    • 2010
  • The utilization of UAV and the activities of diverse widening, now the challenge was how to operate and integrate UAV safely in the NAS. The purpose of this study is to look around the trend for interoperability of UAV in the NAS and to provide its implications and the future direction of integrated operating airspace focusing on U.S. where R&D and demand of UAV are the most in the world.

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Performance Evaluation of Q-Algorithm with Tag Number Estimation Scheme

  • Lim, In-Taek
    • Journal of information and communication convergence engineering
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    • v.8 no.1
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    • pp.45-50
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    • 2010
  • EPCglobal Class-1 Gen-2 standard proposed Q-algorithm to select a frame size for the next query round. Q-algorithm calculates the frame size without estimating the number of tags. Therefore, the Q-algorithm has advantage that the reader's algorithm is simpler than other algorithms. However, it is impossible to allocate the optimized frame size. Also. the conventional Q-algorithm does not define an optimized parameter value C for adjusting the frame size. In this paper, we propose a modified Q-algorithm with the tag number estimation scheme, and evaluate the performance with computer simulations.

Targeted genome engineering via zinc finger nucleases

  • Kim, Seok-Joong;Kim, Jin-Soo
    • Plant Biotechnology Reports
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    • v.5 no.1
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    • pp.9-17
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    • 2011
  • With the development of next-generation sequencing technology, ever-expanding databases of genetic information from various organisms are available to researchers. However, our ability to study the biological meaning of genetic information and to apply our genetic knowledge to produce genetically modified crops and animals is limited, largely due to the lack of molecular tools to manipulate genomes. Recently, targeted cleavage of the genome using engineered DNA scissors called zinc finger nucleases (ZFNs) has successfully supported the precise manipulation of genetic information in various cells, animals, and plants. In this review, we will discuss the development and applications of ZFN technology for genome engineering and highlight recent reports on its use in plants.

Anti-collision Algorithm with Early Cancellation of Query Round in RFID Systems

  • Lim, In-Taek
    • Journal of information and communication convergence engineering
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    • v.7 no.3
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    • pp.292-296
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    • 2009
  • The performance of anti-collision algorithm in RFID systems, which are based on FSA algorithm, may be affected by the frame size a query round. In this paper, an anti-collision algorithm with early cancellation of query round is proposed to enhance the performance of EPCglobal Class-1 Gen-2. The Q-algorithm calculates a Q value to determine the next frame size during a query round. In the proposed algorithm, if the new Q value is different from the previous one, the reader transmits a QueryAdjust command to cancel the current query round. The simulation results show that the proposed algorithm can have a stable performance irrespective of the C value of Q-algorithm and the number of tags.

Technology Trend of Next Gen. PCB/FPCB Copper Foil (차세대 PCB/FPC용 Copper foil 기술 동향)

  • Lee, Seon-Hyeong;Song, Gi-Deok
    • Proceedings of the Korean Institute of Surface Engineering Conference
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    • 2015.11a
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    • pp.158-158
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    • 2015
  • 전해동박(Electrodeposited Copper Foil)은 전기도금 공정으로 제조되는 얇은 구리 포일로서, 주로 TV, PC, 스마트폰 등 전자제품의 인쇄회로기판에서 전기신호를 전달하는 회로소재로 사용이 되며, 최근에는 모바일 IT, 전기자동차, 지능형 로봇, 그린 에너지 산업 등에서 필수적으로 적용되는 소재로 이용이 급증하고 있는 핵심소재이다. 모바일, Network 고속 통신 기술의 발전에 따라 Data 사용량의 폭증으로 고속/고주파 신호전송이 필요성이 증대되고 있으며 무선 충전 기술의 도입 및 웨어러블 기기의 보급으로 점차 FCCL도 3layer에서 2layer로 점차 그 수요가 바뀌어 가고 있다. 이에 따라 전해동박도 고속/고주파 신호 전송 및 고밀도 특성에 맞추어 저조도, 고밀착력 특성을 요구되는 방향으로 개발 되고 있으며 Line Space 가 기존 $25{\mu}m/25{\mu}m$ 패턴에서 $20{\mu}m/20{\mu}m$ 패턴으로 Fine pitch를 요구함에 따라 전해동박의 박막화, 저조도 고 밀착력 특성이 더 요구되고 있다.

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Development of User Interface for Game (게임 개발을 위한 사용자 인테페이스 개발 방법)

  • Kim, SungSu;Kim, SooKyun;An, SungOhk;Kang, Shin-Jin;Jung, JinYoung;Ah, WooYoung
    • Proceedings of the Korean Society of Computer Information Conference
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    • 2014.01a
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    • pp.333-334
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    • 2014
  • 유니티3D는 게임 회사에서 가장 많이 사용하고 있는 게임 엔진 중의 하나이다. 다양한 장르의 게임을 쉽고 간편하게 개발 할 수 있고, 또한 개발비를 절약 할 수 있는 장점을 가지고 있다. 다양한 플랫폼 지원으로 인해 iOS(iPhone Operating System) 환경, 안드로이드(Android) 환경에 맞도록 최적화 하여 플랫폼 전환도 가능하다. 본 논문은 유니티3D에서 강점인 NGUI(Next-Gen UI)의 사용방법과 설계에 대한 방법을 통해서, 사용자 인터페이스 개발에 쉽게 접근 할 수 있는 방법에 대해 소개한다.

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