• Biomolecules & Therapeutics
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• 제27권2호
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• pp.201-209
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• 2019

Mixed lineage leukemia proteins (MLL) are the key histone lysine methyltransferases that regulate expression of diverse genes. Aberrant activation of MLL promotes leukemia as well as solid tumors in humans, highlighting the urgent need for the development of an MLL inhibitor. We screened and isolated MLL1-binding ssRNAs using SELEX (${\underline{S}}ystemic$ ${\underline{E}}volution$ of ${\underline{L}}igands$ by ${\underline{E}}xponential$ enrichment) technology. When sequences in sub-libraries were obtained using next-generation sequencing (NGS), the most enriched aptamers-APT1 and APT2-represented about 30% and 26% of sub-library populations, respectively. Motif analysis of the top 50 sequences provided a highly conserved sequence: 5'-A[A/C][C/G][G/U][U/A]ACAGAGGG[U/A]GG[A/C] GAGUGGGU-3'. APT1, APT2, and APT5 embracing this motif generated secondary structures with similar topological characteristics. We found that APT1 and APT2 have a good binding activity and the analysis using mutated aptamer variants showed that the site information in the central region was critical for binding. In vitro enzyme activity assay showed that APT1 and APT2 had MLL1 inhibitory activity. Three-dimensional structure prediction of APT1-MLL1 complex indicates multiple weak interactions formed between MLL1 SET domain and APT1. Our study confirmed that NGS-assisted SELEX is an efficient tool for aptamer screening and that aptamers could be useful in diagnosis and treatment of MLL1-mediated diseases.

• 한국균학회지
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• 제52권1호
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• pp.61-71
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• 2024

버섯 배지에 첨가되는 재료에 따라 변하는 수확 후 배지내의 세균 군집도를 확인하여 더 넓은 재활용 연구에 기여하고자 춘천, 여주, 홍천, 광주, 의령, 아산에서 수집한 표고, 느타리, 새송이버섯의 수확 후 배지를 대상으로 차세대 염기서열 분석을 진행하였다. ASV 값을 기반으로 α-diversity인 Rarefaction, Chao1, Shannon, Gini-Simpson를 분석한 결과, 유일하게 폐면이 혼합되어 있는 느타리버섯 수확 후 배지인 H-NT 처리구의 세균다양성이 매우 풍부하였다. 또한 β-diversity의 WPGMA를 이용하여 처리구간 세균 군집의 유사성을 분석한 결과, 버섯 종류에 따라 유연관계가 가까운 것을 확인하였다. 본 연구를 시작으로 작물 및 토양에 유용한 특정 세균 비율이 높게 분포하고 있는 수확 후 배지를 연구한다면 맞춤형 유기농자재로서의 활용 가능성이 있음을 시사한다.

• 원예과학기술지
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• 제35권2호
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• pp.149-164
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• 2017

Next generation sequencing (NGS) technologies have led to the rapid accumulation of genome sequences through whole-genome sequencing and re-sequencing of crop species. Genomic resources provide the opportunity for a new revolution in plant breeding by facilitating the dissection of complex traits. Among vegetable crops, reference genomes have been sequenced and assembled for several species in the Solanaceae and Cucurbitaceae families, including tomato, pepper, cucumber, watermelon, and melon. These reference genomes have been leveraged for re-sequencing of diverse germplasm collections to explore genome-wide sequence variations, especially single nucleotide polymorphisms (SNPs). The use of genome-wide SNPs and high-throughput genotyping methods has led to the development of new strategies for dissecting complex quantitative traits, such as genome-wide association study (GWAS). In addition, the use of multi-parent populations, including nested association mapping (NAM) and multiparent advanced generation intercross (MAGIC) populations, has helped increase the accuracy of quantitative trait loci (QTL) detection. Consequently, a number of QTL have been discovered for agronomically important traits, such as disease resistance and fruit traits, with high mapping resolution. The molecular markers for these QTL represent a useful resource for enhancing selection efficiency via marker-assisted selection (MAS) in vegetable breeding programs. In this review, we discuss current genomic resources and marker-trait association analysis to facilitate genome-assisted breeding in vegetable species in the Solanaceae and Cucurbitaceae families.

• 전자공학회논문지S
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• 제36S권6호
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• pp.9-16
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• 1999

이 논문에서는 렘펠의 D-준동현사상을 바탕으로 하여 드브루인 수열을 만드는 효과적인 알고리즘을 제안한다. 이 알고리즘에서는 k차 드브루인 수열에서 n차 드브루인 수열의 다음 비트을 만드는데 필요한 배타논리합 연산수는, r의 2진 표현에서 1의 갯수를 W(r)이라 쓸 때, $k(2^{W(n-k)}-1)$쯤임을 보인다: 딸서, 드브루인 함수를 잘 고르면 이 수는 k가 된다.

• 센서학회지
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• 제21권5호
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• pp.359-366
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• 2012

Nanopore DNA sequencing is an emerging and promising technique that can potentially realize the goal of a low-cost and high-throughput method for analyzing human genome. Especially, solid-state nanopores have relatively high mechanical stability, simple surface modification, and facile fabrication process without the need for labeling or amplification of PCR (polymerized chain reaction) in DNA sequencing. For these advantages of solid-sate nanopores, the use of solid-state nanopores has been extensively considered for developing a next generation DNA sequencing technology. Solid-state nanopore sequencing technique can determine and count charged molecules such as single-stranded DNA, double-stranded DNA, or RNA when they are driven to pass through a membrane nanopore between two electrolytes of cis-trans chambers with applied bias voltage by measuring the ionic current which varies due to the existence of the charged particles in the nanopore. Recently, many researchers have suggested that nanopore-based sensors can be competitive with other third-generation DNA sequencing technologies, and may be able to rapidly and reliably sequence the human genome for under $1,000.

• 자원환경지질
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• 제4권2호
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• pp.77-90
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• 1971

About four hundred deposits of iron, talc, fluorite, tungsten, molybdenum, lead, zinc and other polymetallic mineral deposits were plotted on the Ore Distribution Map of the Ockcheon Geosynclinal Area. These mineral deposits plotted on the map can be divided into the several metallogenic zones by the consideration of their geologic background including the sedimentary and tectonic cycles and the igneous activities in the geosynclinal evolution, as follows: a. Chungju iron and talc zones. b. Cheong-san copper bearing iron sulphide zone c. Kumsan-Muju fluorite-polymetallic zones. d. Cheong-an Puyong and Ein Suckseong gold zone e. Hwang-gan Seolcheon and Sangju gold zones. Chungju iron zone originated in the iron bed in the Kemyongsan Series corresponding to the Pre-Ockcheon Cycle of evolution history. In early period of the Ockcheon Cycle, Hyangsanri quartzite and Munjuri phyllitic formation corresponding to the lower terrigenous sequence were not mineralized while the next sequence of the Samsungsan basic igneous-metamorphic formation and the Changri limestone formation were mineralized by the copper bearing iron sulphide and the fluorite-polymetallic deposits respectively. Two generations of the gold zones are recognized. The earlier generation distributes directionaly in the outside of the Ockcheon sedimentary belt was followed by the earlier grantitic invasion of Jurasic in age, while the later generation scatters at random which was related to the nondirectional Cretaceous granitic intrusion of the Post-Ockcheon Cycle. Conclusively speaking, it was disclosed that the endogenic mineralization in the Ockcheon geosyn clinal zone was not conspicuous in its inner sedimentary belt except its limestone area but in its outer peripheral granitic or gneissic zones, and the related igneous activities occured in the Post-Ockcheon Cycle of evolution history.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.58-58
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• 2017

The advent of next generation sequencing technology has elicited plenty of sequencing data available in agriculturally relevant plant species. For most crop species, it is too expensive to obtain the whole genome sequence data with sufficient coverage. Thus, many approaches have been developed to bring down the cost of NGS. Genotyping-by-sequencing (GBS) is a cost-effective genotyping method for complex genetic populations. GBS can be used for the analysis of genomic selection (GS), genome-wide association study (GWAS) and constructing haplotype and genetic linkage maps in a variety of plant species. For efficiently dealing with plant GBS data, the TASSEL-GBS pipeline is one of the most popular choices for many researchers. TASSEL-GBS is JAVA based a software package to obtain genotyping data from raw GBS sequences. Here, we describe application of GBS and bioinformatics pipeline of TASSEL-GBS for analyzing plant genetics data.

• 한국전기전자재료학회:학술대회논문집
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• 한국전기전자재료학회 2006년도 영호남 합동 학술대회 및 춘계학술대회 논문집 센서 박막 기술교육
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• pp.71-73
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• 2006

This research aims to develop a multiple channel electrochemical DNA chip using micro- fabrication technology. At first, we fabricated a high integrated type DNA chip array by lithography technology. Several probe DNAs consisting of thiol group at their 5-end were immobilized on the gold electrodes. Then target DNAs were hybridized by an electrical force. Redox peak of cyclic-voltammogram showed a difference between target DNA and mismatched DNA in the anodic peak current. Therefore. it is able to detect a various genes electrochemically after immobilization of a various probe DNA and hybridization of label-free DNA on the electrodes simultaneously. It suggested that this DNA chip could recognize the sequence specific genes.

• 디지털콘텐츠학회 논문지
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• 제8권3호
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• pp.385-391
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• 2007

HFC 망 상향채널의 용량을 증대시키기 위한 차세대 케이블 모뎀 기술의 하나인 S-DMT 방식 케이블 모뎀의 주파수 동기 알고리즘 제안하고 성능을 평가하였다. 다수 반송파 전송 시스템의 주파수 동기 알고리즘들에 대한 분석을 통하여, 데이터 프레임의 프리앰블 필드 내에 약속된 반복 패턴의 훈련열을 삽입하여 전송하고, 파일롯 신호를 이용하여 잔류 주파수 오프셋에 따른 위상오차를 보정하는 방법을 제안하고 시뮬레이션을 통해 AWGN 잡음 환경에서의 성능을 평가 하였다. 성능 평가 결과, 파일롯 신호의 수와 크기에 따라 성능 차이를 보이나, 제안한 주파수 동기 알고리즘이 신속한 동기를 필요로 하는 S-DMT 방식 상향채널 케이블 모뎀에 적합하고 양호한 성능을 보임을 확인하였다.

• 식물분류학회지
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• 제46권1호
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• pp.60-64
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• 2016

Scopolia parviflora of the family Solanaceae is an endemic species of Korea and a traditional Korean medicinal plant. The plastid genome was sequenced by next-generation sequencing (NGS) method. The characterized cp genome is 156,193 bp in size; the large single-copy (LSC) region is 86,364 bp, the inverted repeat (IR) is 25,905 bp, and the small single copy (SSC) region is 18,019 bp. The overall GC content of the plastid genome amounts to 37.61%. The cp genome contains 113 genes and 21 introns, including 80 proteincoding genes, four RNA genes, 30 tRNA genes, 20 group II introns, and one group I intron. A phylogenetic analysis showed that Scopolia parviflora was closely related to Hyoscyamus niger.