• Plant Biotechnology Reports
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• v.2 no.2
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• pp.93-112
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• 2008

Medicinal and aromatic plants (MAPs) are important sources for plant secondary metabolites, which are important for human healthcare. Improvement of the yield and quality of these natural plant products through conventional breeding is still a challenge. However, recent advances in plant genomics research has generated knowledge leading to a better understanding of the complex genetics and biochemistry involved in biosynthesis of these plant secondary metabolites. This genomics research also concerned identification and isolation of genes involved in different steps of a number of metabolic pathways. Progress has also been made in the development of functional genomics resources (EST databases and micro-arrays) in several medicinal plant species, which offer new opportunities for improvement of genotypes using perfect markers or genetic transformation. This review article presents an overview of the recent developments and future possibilities in genetics and genomics of MAP species including use of transgenic approach for their improvement.

• Archives of Pharmacal Research
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• v.10 no.2
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• pp.103-109
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• 1987

To find microorganisms producing esterase inhibitors, microbes were isolated from soil samples that were collected at different locations in Korea and screened for inhibitory activities. One of the inhibitor-producing strains was named strain DMC-498. This strain was found to be a new species of the genus Streptomyces by comparison with the characteristics of morphology and metabolisms of the other species of the genus.

• Food Science and Biotechnology
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• v.14 no.3
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• pp.416-419
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• 2005

Unknown bacterium isolated from garlic was characterized using phenotypic methods, phylogenetic analysis, DNA-DNA hybridization, and cultural methods. The strain was identified as typical leuconostoc; Gram-positive, non-sporeforming, heterofermentative, catalase-negative and spherical. Although its 16S rRNA gene sequence showed high homology to Leuconostoc argentinum DSM $8581^T$(99.8%), DNA-DNA hybridization experiments indicated it represents novel genomic species in the genus Leuconostoc. The garlic-specific leuconostoc was more resistant to antimicrobial activity of garlic compared to other common laboratory lactic acid bacteria, and was even stimulated by low concentrations (1-2%) of garlic extract supplemented in trypticase soy broth. Growth stimulation was concentration-dependent when tested with residual aqueous layer after solvent extraction of fresh whole garlic extract.

• Korean journal of applied entomology
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• v.57 no.4
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• pp.275-278
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• 2018

An aquatic wasp, Agriotypus silvestris Konishi & Aoyagi, 1994 is newly recognized in South Korea. A diagnosis and photographs of the diagnostic characters for the species and an identification key to the South Korean Agriotypus species are provided herein.

• Animal Systematics, Evolution and Diversity
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• v.38 no.3
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• pp.122-129
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• 2022

Scolelepis (Scolelepis) lingulata is newly reported in Korean waters. This species, collected from the intertidal zone of the Yellow Sea, is in good agreement with the original and subsequent descriptions in presence of occipital antenna, conspicuously large and lingulate interramal lobes in posterior chaetigers, absence of hooded hooks in notopodia, and unidentate hooded hooks present in neuropodia. Additionally, Korean specimens previously identified as Scolelepis(Scolelepis) kudenovi are reexamined based on the morphology. As a result, these specimens were identified as Scolelepis (Scolelepis) daphoinos in terms of the presence of reddish-brown pigmentation along the transverse ciliated bands on the dorsum. The identification key to Scolelepis species from Korean waters is provided.

• Ocean and Polar Research
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• v.29 no.3
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• pp.273-282
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• 2007

For an efficient management and utilization of marine biodiversity information, we made an attempt to develop the Korea Marine Biodiversity Information System (KoMBIS), building a species name inventory of Korea marine organisms. The inventory includes 17 organism groups: phytoplankton, zooplankton, algae and halophyte, sponges, cnidarians, rotifers, nematodes, bryozoans, brachiopods, molluscs, echiurans, annelids, arthropods, echinoderms, urochordates and fish. The species names were collected from 37 different references and reviewed for validity by taxonomists, which resulted in 9,798 valid names in addition to 1,845 synonyms. The Korea marine species inventory is the first one of this kind, for previous Korean species name inventories were mostly composed of terrestrial and freshwater organisms. KoMBIS, the information system developed, contains not only the species name but also information on morphological and ecological characteristics such as distribution, DNA barcode, and references. This system is convenient for the inputting of new data and servicing users through the internet, so that management and utilization of the biodiversity information is more efficient. Linking the DNA barcode data with species information provides an objective measure for identification of a species, which accommodates the recommendation of Consortium for the Barcode of Life, and makes the Korea marine biodiversity information compatible with international databases. Considering the frequent exchange of marine organisms internationally via ballast water and such issues as climate change, this information system will be useful in many areas of marine biodiversity.

• International Journal of Computer Science & Network Security
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• v.21 no.2
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• pp.205-211
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• 2021

Plants are important for humanity. They intervene in several areas of human life: medicine, nutrition, cosmetics, decoration, etc. The large number of varieties of these plants requires an efficient solution to identify them for proper use. The ease of recognition of these plants undoubtedly depends on the classification of these species into family; however, finding the relevant characteristics to achieve better automatic classification is still a huge challenge for researchers in the field. In this paper, we have developed a new automatic plant classification technique based on artificial neural networks. Our model uses leaf texture characteristics as parameters for plant family identification. The results of our model gave a perfect classification of three plant families of the Ivorian flora, with a determination coefficient (R2) of 0.99; an error rate (RMSE) of 1.348e-14, a sensitivity of 84.85%, a specificity of 100%, a precision of 100% and an accuracy (Accuracy) of 100%. The same technique was applied on Flavia: the international basis of plants and showed a perfect identification regression (R2) of 0.98, an error rate (RMSE) of 1.136e-14, a sensitivity of 84.85%, a specificity of 100%, a precision of 100% and a trueness (Accuracy) of 100%. These results show that our technique is efficient and can guide the botanist to establish a model for many plants to avoid identification problems.

• Korean Journal of Ichthyology
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• v.19 no.4
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• pp.274-285
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• 2007

The subfamily Chrominae of damselfishes (Teleostei: Pomacentridae) includes the genus Chromis and Dascyllus. They are found throughout the tropical oceans and form a major component of coral reef communities. There are 5 species of the Chrominae currently recognized in Korea. This study was conducted to infer phylogenetic position of two Korean Chromis species and one Dascyllus species within general category of their each genus in worldwide level. This study also includes one species of Japanese Dascyllus. In the phylogenetic analysis, the Japanese D. aruanus grouped with D. aruanus previously reported from French Polynesia. Korean Chromis fumea grouped with Australian C. nitida and the p-distance value between the two species is relatively very low (0.047). Korean C. notatus grouped together with C. flavomaculata (New Caledonia). In the sequence analysis of some Korean and Japanese damselfishes, there was no sequence variation between D. melanurus (Jeju, Korea) and D. melanurus (Indo-Pacific), but the sequences of the two populations were different in only one nucleotide sites from that of D. melanurus in Indonesian Archipelago. The sequences of Dascyllus aruanus (Japan) were different in two nucleotide sites from it in French Polynesia. There were high difference between the sequences of two Korean species, Chromis fumea and Korean C. notatus. The variations among mitochondrial cytochrome b sequences indicate that the gene sequence could be used as DNA barcode for identification of local populations of D. aruaus and D. melanurus as well as species level.

• Proceedings of the Korean Vacuum Society Conference
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• 2016.02a
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• pp.384.1-384.1
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• 2016

We reported that amorphous silicon (a-Si) thin film provide sample plate exhibiting a multimodality to measure biomolecules by secondary ion mass spectrometry (SIMS) and laser desorption/ionization mass spectrometry (LDI-MS). Kim et al.1 reported that a-Si thin film were suitable to detect small molecules such as drugs and peptides by SIMS and LDI-MS. Recently, bacterial identification has been required in many fields such as food analysis, veterinary science, ecology, agriculture, and so on.2 Mass spectrometry is emerging for identifying and profiling microbiology samples from its advantageous characters of label-free and shot-time analysis. Five species of bacteria - S. aureus, G. glutamicum, B. kurstaki, B. sphaericus, and B. licheniformis - were sampled for MS analysis without lipid extraction in sample preparation steps. The samples were loaded onto the a-Si thin film with a thickness of 100 nm which did not only considered laser-beam penetration but also surface homogeneity. Mass spectra were recorded in both positive and negative ionization modes for more analytical information. High reproducibility and sensitivity of mass spectra were demonstrated in a mass range up to mass-to-charge ratio(m/z) 1200 by applying the a-Si thin film in mentioned above MS. Principle component analysis (PCA) - a popular statistical analysis widely used in data processing was employed to differentiate between five bacterial species. The PCA results verified that each bacterial species were readily distinguished and differentiated effectively from our MS approach. It shows a new opportunity to rapid bacterial profiling and identification in clinical microbiology. More details will be discussed in the presentation.

• Journal of Food Hygiene and Safety
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• v.16 no.4
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• pp.251-257
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• 2001

Listeria monocytogenes and L. ivanovii are important food-pathogens for human and animal. The diagnostic of Listeria in food using culture medium requires time and laborwork, because there are many other non-pathogenic species like L. innocua, L welshimeri, L. seeligeri and L. grayi in Genus Listeria. For these reasons, Lismix multiplex PCR method was developed as a rapid method for the detection and identification of Listeria. In this study we developed a practical system of Lis-mix PCR detection for the application to food samples and new developed Siw-mix III PCR system overall 69 listerial strains were successful species-identified and confirmed. Also, the Siw-mix III PCR system allows the species-specific identification among L. ivanovii, L. welshimeri and L seeligeri in a single PCR.