• Title/Summary/Keyword: New marker and test

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Application of UPOV Data for the Analysis of Genetic Variation in Rose Cultivars

  • Kim, Gi-Jun;Song, Young-Ha;Gi, Gwang-Yeon;Kim, Seong-Tae;Lee, Ja-Hyun;Han, Tae-Ho
    • Horticultural Science & Technology
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    • v.29 no.3
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    • pp.240-246
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    • 2011
  • The principal objective of this study was to estimate the availability of morphological data on the basis of the guidelines of the International Union for the Protection of New Varieties of Plants (UPOV) with regard to the identification of the rose germplasm. The correlation of morphological traits and random amplified polymorphic DNA (RAPD) marker data among 44 rose cultivars was assessed via a mantel test. Thirty eight phenotypes were employed for morphological analysis. Sixteen primers were utilized for RAPD analysis, and these generated 225 polymorphic bands. The dendrogram based on the RAPD markersgrouped 44 rose cultivars according to their horticultural types. No significant correlation was observed between the morphological and RAPD marker data. We concluded that current UPOV traits could not be applied to study genetic variation. Further studies on morphological traits are required for the analysis of genetic variation among cultivars.

A Watershed-based Texture Segmentation Method Using Marker Clustering (마커 클러스터링을 이용한 유역변환 기반의 질감 분할 기법)

  • Hwang, Jin-Ho;Kim, Won-Hee;Moon, Kwang-Seok;Kim, Jong-Nam
    • Journal of Korea Multimedia Society
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    • v.10 no.4
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    • pp.441-449
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    • 2007
  • In clustering for image segmentation, large amount of computation and typical segmentation errors have been important problems. In the paper, we suggest a new method for minimizing these problems. Markers in marker-controlled watershed transform represent segmented areas because they are starting-points of extending areas. Thus, clustering restricted by marker pixels can reduce computational complexity. In our proposed method, the markers are selected by Gabor texture energy, and cluster information of them are generated by FCM (fuzzy c-mean) clustering. Generated areas from watershed transform are merged by using cluster information of markers. In the test of Brodatz' texture images, we improved typical partition-errors obviously and obtained less computational complexity compared with previous FCM clustering algorithms. Overall, it also took regular computational time.

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Implementation of AR based Assembly System for Car C/pad Assembly (차체 C/Pad 조립을 위한 증강현실 기반의 조립시스템 구현)

  • Park, Hong-Seok;Choi, Hung-Won;Park, Jin-Woo
    • Journal of the Korean Society for Precision Engineering
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    • v.25 no.8
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    • pp.37-44
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    • 2008
  • Nowadays, the increasing global competition forces manufacturer to reduce the cost and time for implementation of manufacturing system. The AR(augmented reality) technology as a new human-machine interface introduces a noteworthy perspective for a new manufacturing system design. Using AR technology, a physically existing production environment can be superimposed with virtual planning objects. Therefore, the planning tasks can be validated without modeling the surrounding environment of the production domain during short process planning time. In this paper, we introduce the construction of AR browser and determine the optimal environment parameters for field application of AR system through lots of tests. And, many methods such as multi-marker coordinate system, division of virtual objects and so on, are proposed in order to solve the problems suggested from initial field test. Based on these tests and results, the test-bed of C/Pad assembly system is configured and robot program for C/Pad assembly is generated based on AR system.

Infection and cox2 sequence of Pythium chondricola (Oomycetes) causing red rot disease in Pyropia yezoensis (Rhodophyta) in Korea

  • Lee, Soon Jeong;Jee, Bo Young;Son, Maeng-Hyun;Lee, Sang-Rae
    • ALGAE
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    • v.32 no.2
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    • pp.155-160
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    • 2017
  • Red rot disease has caused a major decline in Pyropia (Nori) crop production in Korea, Japan, and China. To date, only Pythium porphyrae (Pythiales, Oomycetes) has been reported as the pathogen causing red rot disease in Pyropia yezoensis (Rhodophyta, Bangiales). Recently, Pythium chondricola was isolated from the infected blades of Py. yezoensis during molecular analyses using the mitochondrial cox1 region. In this study, we evaluated the pathogenicity of P. chondricola as an algal pathogen of Py. yezoensis. Moreover, a new cox2 marker was developed with high specificity for Pythium species. Subsequent to re-inoculation, P. chondricola successfully infected Py. yezoensis blades, with the infected regions containing symptoms of red rot disease. A novel cox2 marker successfully isolated the cox2 region of Pythium species from the infected blades of Py. yezoensis collected from Pyropia aquaculture farms. cox2 sequences showed 100% identity with that of P. chondricola (KJ595354) and 98% similarity with that of P. porphyrae (KJ595377). The results of the pathogenicity test and molecular analysis confirm that P. chondricola is a new algal pathogen causing red rot disease in Pyropia species. Moreover, it could also suggest the presence of cryptic biodiversity among Korean Pythium species.

New Techniques for the Detection of the Malignant Cells in Urine Cytology (요 세포검사의 최근 검사기법)

  • Gong, Gyung-Yub
    • The Korean Journal of Cytopathology
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    • v.17 no.1
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    • pp.18-26
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    • 2006
  • Transitional cell carcinoma of the urinary bladder is common in the genitourinary tract. The gold standard for the diagnosis of bladder cancer has been cystoscopy, along with urine cytology. Cystoscopy is an invasive and relatively expensive technique. By comparison, urine cytology is easy to perform and specific for a diagnosis of bladder cancer, although less sensitive, especially in low-grade tumors. For this reason, there has been a need for superior noninvasive technology to increase our confidence in being able to detect bladder cancer. There are many reports of the various urinary tests that are available to facilitate the diagnosis. In this article, I reviewed the literature on urinary markers and tests that may be clinically useful, including fluorescence in situ hybridization, uCyt+/Immunocyte, the $BTA^{(R)}$ test, the NMP 22TM, the $FDP^{(R)}$ test, the telomerase activity test, the HA and HAse tests, and flow cytometry. Most of these tests have a higher sensitivity and specificity than cytology. However, urine cytology has the highest specificity, especially in individuals with a high-grade tumor. We conclude that no urinary markers or tests can replace the role of cystoscopy along with cytology in the diagnosis of transitional cell carcinoma of the bladder. However, some markers could be used adjunctively to increase the diagnostic accuracy during screening or during the postoperative follow-up examination of patients with bladder cancer.

Establishment of content criteria of marker compounds through the monitoring of Achyranthis Radix collected from Korea and China (한국 및 중국 지역에서 수집된 우슬의 모니터링을 통한 지표성분의 함량기준 설정)

  • Kim, Dae-Hyun;Kim, Sang-Hyuk;Jang, Yu-Seon;Shin, Min-Chul;Chu, Van Men;Lee, Young-Keun;Woo, Mi-Hee;Kang, Jong-Seong
    • Analytical Science and Technology
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    • v.25 no.4
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    • pp.250-256
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    • 2012
  • Two marker compounds of Achyranthis Radix, ecdysterone and inokosterone, were analyzed by HPLC on an ODS column ($250{\times}4.6$ mm, 5 ${\mu}m$) with a mobile phase of 15% acetonitrile containing 0.08% formic acid at a flow rate of 1.0 mL/min and a detection wavelength of UV 254 nm. The method was validated by ICH guideline and applied to the monitoring of marker compounds in 93 samples of Achyranthis Radix collected at various areas in Korea and China. The new content criteria of ecdysterone and inokosterone, established using linear regression method were 0.033% and 0.020%, respectively. When the new content criteria were applied to the quality control test of commercial Achyranthis Radix, 95.4% of total samples including 100% of Korean and 92.6% of Chinese samples were passed the test. Application of new content criteria could protect the Korean products and decrease the distribution of Chinese products with lower quality.

Studies on the Identification Test of Herbal Medicines and its Preparations (한약엑스제의 확인시험법에 관한 연구)

  • Kim, Hye-Jin;Suh, Yong-Taek;Lee, Jong-Pill;Cho, Jung-Hee;Jang, Young-Pyo
    • Korean Journal of Pharmacognosy
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    • v.40 no.2
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    • pp.155-160
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    • 2009
  • The reference herbal extracts and their identification methods by Korean Pharmacopoeia IX were established based on the organic solvent extracts to detect their marker compounds. However, most of herbal medicine decoctions in the market are prepared with water as extracting solvent. As the reference herbal extracts and their identification methods are not appropriate for the practical test, new preparation of alternative reference herbal extracts and their identification test methods are essential for the correction of test methods and identification of herbal drugs. Three novel identification test methods were developed for Evodiae Fructus, Cimicifugae Rhizoma and Aurantii Fructus Immaturus. Total 10 reference herbal extracts and their identification methods were optimized for Ephedrae Herba, Schisandrae Fructus, Curcuma longae Rhizoma, Citri Unshius Pericarpium, Puerariae Radix, Corni Fructus, and Cinnamomi Cortex.

Untouched Camera Mouse System for The Disabled Person's Assistant Device (뇌병변 장애인 보조기기용 노터치 카메라 마우스 시스템)

  • Bae, Ki-Tae
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.11 no.9
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    • pp.3465-3471
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    • 2010
  • In this paper, we propose assistant device system based on camera that can perform general mouse function for the physically disabled. Most disabled people use special hardware and rely largely on foreign-made assistance device and the using rate of assistance device was very low because of high price. To solve this problem, we propose effective system based on web camera that can perform general mouse function and can raise accessibility for the physically disabled. In the proposed method, we firstly attach color marker on arbitrary device or body parts and then input the information of color using camera and extract coordinate information of color marker and finally, match processing information(coordinate) to special event(keyboard, mouse) and control various applications. The proposed method shows good performance in price and performance compared with other techniques by real field test.

Breeding of a New Silkworm Variety, Kumhwangjam, with a Sex-Limited Cocoon Color for Spring Rearing Season

  • Kang, Pil Don;Sohn, Bong Hee;Lee, Sang Uk;Kim, Mi Ja;Jung, I Yeon;Kim, Young Soon;Kim, Yung Dae;Lee, Heui Sam
    • International Journal of Industrial Entomology and Biomaterials
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    • v.9 no.1
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    • pp.89-93
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    • 2004
  • A new silkworm variety, Kumhwangjam, with a sex-limited cocoon color for spring rearing season is F$_1$ hybrid between Jam 309, a Japanese race bred from Y1/9153, and Jam 310, a Chinese race from Y1/8586. In the local adaptability test performed at 8 local areas in spring rearing season of 2003, major characteristics of Kumhwangjam were mainly similar to those of the check variety Kumokjam. The new silkworm variety, Kumhwangjam, showed a sex-limited cocoon color: yellow for female and white for male with elliptical cocoon shape. This sex-limited cocoon color can be used as a valuable marker for discrimination between female and male for various purposes.

Vision-based Human-Robot Motion Transfer in Tangible Meeting Space (실감만남 공간에서의 비전 센서 기반의 사람-로봇간 운동 정보 전달에 관한 연구)

  • Choi, Yu-Kyung;Ra, Syun-Kwon;Kim, Soo-Whan;Kim, Chang-Hwan;Park, Sung-Kee
    • The Journal of Korea Robotics Society
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    • v.2 no.2
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    • pp.143-151
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    • 2007
  • This paper deals with a tangible interface system that introduces robot as remote avatar. It is focused on a new method which makes a robot imitate human arm motions captured from a remote space. Our method is functionally divided into two parts: capturing human motion and adapting it to robot. In the capturing part, we especially propose a modified potential function of metaballs for the real-time performance and high accuracy. In the adapting part, we suggest a geometric scaling method for solving the structural difference between a human and a robot. With our method, we have implemented a tangible interface and showed its speed and accuracy test.

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