• Korean Journal of Veterinary Service
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• v.14 no.1
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• pp.19-26
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• 1991

A considerably high rate of abnormal deliveries of unknown etiology was observed among dairy cattles from November 1988 to February 1989 and Korean native tattles from January to April 1990. The abnormal deliveries consisted of abortions, stillbirths and calf deformities refers to as congenital arthrogryposis hydranencephaly (AH )syndrome. In order to know the level of Akabane antibody of dairy cattle raised in Kyungbuk province, serum neutralization test was conducted with Akabane virus(OBE-1 strain) and HmLu(Hamster lung) cell line. The results were summarized as follows. 1. During 4 months(Nov. 1988-Feb. 1989), abortion (3 heads), stillbirth(1 head) and congenital abnormalities(13 heads) of newborn were occurred in 17 dairy cattles raised in Kyungbuk province. 2. During 4 months(Jan.-Apr.1990), stillbirth(2 heads) and congenital deformities (13 heads) of newborn were occurred in 15 Korean native tattles raised in Kyungbuk province. 3. In Fev, and Apr. 1990, 1,005 dairy cattles at 99 farms were investigated on the actual condition of possessing Akabane antibody. The result was that 1,000 heads (99.9%) in 1,005 dairy cattles reacted as positive condition in Akahane antibody. The antibody titer was from 4 to over 256. 4. 189 heads (18.8%) of 1,005 dairy cattles werw below antibody titer 8 and 816 heads (81.2%) were over 16. 5. Akabane antibody titer of east coast legions(Pohang Yeongil etc) was all over 16, that of internal legions (Yeongiu, Andong. etc) was relatively low, The result suggest that the vaccination for Akabane disease will be unnecessary for the time being because of possessing higher antibody titer reaction except the newly introduced cattle and Akabane virus was widely disseminated in kyungbuk province during the summer months in 1987 or 1988.

• IMMUNE NETWORK
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• v.9 no.1
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• pp.27-33
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• 2009

Macrophages generated in vitro using macrophage-colony stimulating factor (M-CSF) and interleukin (IL)-6 from bone marrow cells (BM-Mp) are defective in antigen presenting cell (APC) function as shown by their ability to induce the proliferation of anti-CD3 mAb-primed syngeneic T cells. However, they do express major histocompatibility (MHC) class I and II molecules. accessory molecules and intracellular adhesion molecules. Here we demonstrate that the defective APC function of macrophages is mainly due to production of $TGF-{\beta}1$ by BM-Mp. Methods: Microarray analysis showed that $TGF-{\beta}1$ was highly expressed in BM-Mp, compared to a macrophage cell line, B6D. which exerted efficient APC function. Production of $TGF-{\beta}1$ by BM-Mp was confirmed by neutralization experiments of $TGF-{\beta}1$ as well as by real time-polymerase chain reaction (PCR). Results: Addition of $anti-TGF-{\beta}1$ monoclonal antibody to cultures of BM-Mp and anti-CD3 mAb-primed syngeneic T cells efficiently induced the proliferation of syngeneic T cells. Conversely, the APC function of B6D cells was almost completely suppressed by addition of $TGF-{\beta}1$. Quantitative real time-PCR analysis also confirmed the enhanced expression of $TGF-{\beta}1$ in BM-Mp. Conclusion: The defective APC function of macrophages generated in vitro with M-CSF and IL-6 was mainly due to the production of $TGF-{\beta}1$ by macrophages.

• Journal of Korean Society for Atmospheric Environment
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• v.33 no.5
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• pp.445-457
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• 2017

In this study, we developed an approach to better account for uncertainties in estimated contributions from fine particulate matter ($PM_{2.5}$) modeling. Our approach computes a Concentration Correction Factor (CCF) which is a ratio of observed concentrations to baseline model concentrations. We multiply modeled direct contribution estimates with CCF to obtain revised contributions. Overall, the modeling system showed reasonably good performance, correlation coefficient R of 0.82 and normalized mean bias of 2%, although the model underestimated some PM species concentrations. We also noticed that model biases vary seasonally. We compared contribution estimates of major source sectors before and after applying CCFs. We observed that different source sectors showed variable magnitudes of sensitivities to the CCF application. For example, the total primary $PM_{2.5}$ contribution was increased $2.4{\mu}g/m^3$ or 63% after the CCF application. Out of a $2.4{\mu}g/m^3$ increment, line sources and area source made up $1.3{\mu}g/m^3$ and $0.9{\mu}g/m^3$ which is 92% of the total contribution changes. We postulated two major reasons for variations in estimated contributions after the CCF application: (1) monthly variability of unadjusted contributions due to emission source characteristics and (2) physico-chemical differences in environmental conditions that emitted precursors undergo. Since emissions-to-$PM_{2.5}$ concentration conversion rate is an important piece of information to prioritize control strategy, we examined the effects of CCF application on the estimated conversion rates. We found that the application of CCFs can alter the rank of conversion efficiencies of source sectors. Finally, we discussed caveats of our current approach such as no consideration of ion neutralization which warrants further studies.