• Asian-Australasian Journal of Animal Sciences
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• v.13 no.2
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• pp.188-191
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• 2000

Appetite stimulant ether-soluble neutral extract of a high-grain compound feed was separated into four fractions with an open column of silica gel chromatograph using four carrier solutions containing n-pentane and diethyl ether as 100:0 (Fraction A), 90:10 (Fraction B), 75:25 (Fraction C) and 0:100 (Fraction D). The stimulative effects of the fractions were examined by comparing the intake of hay (with or without the fractions) in two-choice test bioassays with cattle. The Fractions A, B and C of the neutral extract stimulated (p<0.05) hay consumption in cattle, whereas Fraction D had no effect on selective feeding when compared with the control fraction. Furthermore, Fractions A, B and C had higher (p<0.05) feeding stimulative indices (FSI) than that of the Fraction D. The results suggest that chemical stimulants to increase palatability of hay are present in the neutral Fractions A, B and C of high-grain concentrate.

• Proceedings of the Korea Society for Industrial Systems Conference
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• 2003.11a
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• pp.721-727
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• 2003

This study designed and implemented a system to extract of vowel from a word. The system is comprised of a voice feature extraction module and a neutral network module. The voice feature extraction module use a LPC(Linear Prediction Coefficient) model to extract a voice feature from a word. The neutral network module is comprised of a learning module and voice recognition module. The learning module sets up a learning pattern and builds up a neutral network to learn. Using the information of a learned neutral network, a voice recognition module extracts a vowel from a word. A neutral network was made to learn selected vowels(a, eo, o, e, i) to test the performance of a implemented vowel extraction recognition machine. Through this experiment, could confirm that speech recognition module extract of vowel from 4 words.

• Journal of the Korean Wood Science and Technology
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• v.48 no.6
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• pp.878-887
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• 2020

A neutral extract (NE), that is soluble in cold water and has excellent antioxidant activity, from Pinus radiata pine bark was prepared by sodium bicarbonate treatment, and its chemical characteristics were investigated. NE was prepared by treating P. radiata bark with 0.8% NaHCO₃ aqueous solution with a 5 : 1 liquor-to-bark ratio at boiling temperature for 1 h, resulting in 44% yield and final pH of 6.66. The yield of NE was 11% higher than that of the hot water extract (HWE) due to the increase in the solubility of polyphenols, the main component in the bark, by NaHCO₃ treatment. NE was characterized through FT-IR, NMR, and MALDI TOF MS analyses. The results indicated that NE is mostly composed of proanthocyanidins (PAs) consisting of procyanidin (PC) units. The acetylated neutral extract (Ac-NE) had weight average molecular weight (${\bar{M}}w$) of 5,300 Da. The Ac-NE had wide molecular weight distribution and its polydispersity (${\bar{M}}w/{\bar{M}}n$) was 6 times higher than that of pure PA. The antioxidant activity of NE was determined by 2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assay and showed that NE had comparable antioxidant activity with pure PA.

• Journal of Microbiology and Biotechnology
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• v.11 no.3
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• pp.406-411
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• 2001

In a bacterial denitrification test with Pseudomonas sp. and anaerobic consortium, more nitrates and less substrate were consumed but less metabolic nitrite was produced under an anaerobic $H_2$ condition rather than under $N_2$ condition. In a bioelectrochemical denitrification test with the same organisms, the electrochemically reduced neutral red was confirmed to be a substitute electron donor and a reducing power like $H_2$. The biocatalytic activity of membrane-free bacterial extract, membrane fraction, and intact cell for bioelectrochemical denitrification was measured using cyclic voltammetry. When neutral red was used as an electron mediator, the electron transfer from electrode to electron acceptor (nitrate) via neutral red was not observed in the cyclic voltammogram with the membrane-free bacterial extract, but it was confirmed to gradually increase in proportion to the concentration of nitrate in that of the membrane fraction and the intact cell of Pseudomonas sp.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.35 no.3
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• pp.193-202
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• 2009

This study was done to assess an alternative method as a replacement of in vivo phototoxicity test. The human fibroblasts were exposed to several phototoxic chemicals (promethazine, chlorpromazine chlortetracycline, 8-methoxypsoralen, neutral red, bithionol) and non-phototoxic materials (cinnamic aldehyde, p-aminobenzoic acid, sodium lauryl sulfate, L-cysteine). The cell viability was measured by neutral red uptake (NRU) assay. The results of the NRU phototoxicity (PT) assay showed a close agreement with in vivo test except bithionol. We also have tested the cosmetic ingredients including $Medimin^{(R)}$ A, $Medimin^{(R)}$ D, $LG^{(R)}$ 106W, $Phytoclear^{(R)}$ EL-1, Carex humilis L. extract, Canna indica L. extract, Salvia miltiorrhira Bunge extract, $Parsol^{(R)}$ MCX and $Parsol^{(R)}$ 1789. Most materials except Salvia miltiorrhira Bunge extract did not show any phototoxicity.

• Applied Biological Chemistry
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• v.29 no.2
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• pp.138-147
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• 1986

Barley koji was made in order to investigate the lipid contents of barely koji during preparation. Diethyl ether extracts and 85% methanol extracts were extracted and purified. The lipid components were classified. The individual neutral lipids, glycolipids and phospholipids were fractionted, quantified and fatty acid compositions of the three lipids were determined. Total lipid contents of diethyl ether and 85% methanol extract of barley koji increased during preparation. Neutral lipid, glycolipid and phospholipid contents in diethyl ether extract increased, however, neutral lipid, glycolipid and phospholipid contents in 85% methanol extract decreased during koji preparation. TG content of the neutral lipid in diethyl ether extract decreased. Conversely, DG, FS, FFA and ES contents increased. But TG, DG and FS contents of the neutral lipid in 85% methanol extract decreased. LPC, (PC+PS), PI, PG and PE contents of the phospholipid on diethyl ether extract increased. But LPC, (PC+PS), PE and PI contents in 85% methanol extract decreased during koji preparation. Palmitic acid, linoleic acid and linolenic acid of neutral lipid in diethyl ether extract decreased, however, palmitic, stearic, oleic, linoleic acid in 85% methanol extract decreased. Palmitic acid, stearic acid, oleic acid and linoleic acid of glycolipid in diethyl ether extract increased, but in 85% methanol extract they decreased except oleic acid. Palmitic, stearic, oleic, linoeic and linolenic acid of phospholipid in diethyl ether extract increased during koji preparation. On the other hand palmitic, oleic and linoleic acid in 85% methanol extract decreased but stearic and linoleic acid increased.

• Korean Journal of Pharmacognosy
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• v.17 no.2
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• pp.184-188
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• 1986

The preliminary antifertility test of Dictamnus albus(Rutaceae) showed that a methanol extract of root bark decreased fertility in rats when administered orally on days $1{\sim}10$ postcoitum. Hexane, chloroform, butanol and aqueous fractions from the methanol extract, were tested to produce the result that only the hexane fraction exhibited positive activity. The hexane fraction was subfractionated into acidic, alkaloidal and neutral subtractions and tested. Neutral subtraction showed positive activity. From the neutral subtraction, fraxinellone was isolated as an active principle. The timed-dosing experiments revealed that antifertility activity of fraxinellone appeared to be exerted after tubal exit of the ova to prevent implantations.

• The Korean Journal of Food And Nutrition
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• v.9 no.4
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• pp.472-477
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• 1996

This study was conducted to investigate the effect of old antler extracts on galactosamine-induced liver injuries in rats. Male rats of Sprague-Dawley strain with average weight of 110$\pm$10g were fed on diets containing three kinds of old antler extracts(water extract, neutral extract and ether extract) for four weeks. Galactosamine(400mg/kg body weight) was injected intraperitoneally at the same time every week in galactosamine treatment groups. Cytochrome P-450 content was decreased in galactosamine treatment groups and increased by old antler extracts administration. Glutathione-peroxidase activity was increased in water extract group. Hepatic glutathione content was not observed significant differences by the old antler extracts administration. Lipid peroxide content was higher in the galactosamine treatment groups than that of the control group and decreased in galactosamine administerd groups after pretreatment with water extract. Total lipid, triglyceride and total cholesterol contents of liver were decreased in old antler extracts administerd groups and decreased in water extract group.

• Journal of the Korean Wood Science and Technology
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• v.49 no.2
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• pp.134-141
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• 2021

This study was conducted to investigate the potential of the neutral extract (NE) prepared by treating P. radiata bark with aqueous NaHCO3 solution as a natural dyestuff. Silk and cotton fabrics were dyed at four different NE concentrations, 0.5%, 1.0%, 1.5%, and 2.0% o.w.b. (on the weight of bath). The fabric to liquid ratio of 1 : 50, dyeing temperature of 90℃, and dyeing time of 90 min were used as the fixed condition. The appropriate NE dyeing concentration of silk and cotton fabrics was 1.5% o.w.b. Colors of the dyed silk and cotton fabrics were brown and light brown, respectively. From colorfastness assessment, both dyed silk and cotton showed good washing and rubbing fastness, but poor light fastness. NE dyed fabrics exhibited excellent ammonia deodorization property and antibacterial activity against Staphylococcus aureus and Klebsiella pneumoniae. Therefore, NE of P. radiata bark could have the potential for use as a functional brown dyestuff.

• Journal of Pharmaceutical Investigation
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• v.40 no.3
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• pp.187-192
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• 2010

We aimed to evaluate the effect of temperature, pH, and light conditions on the stability of porcine placental extract (PPE)-loaded liposomes with different surface charges. The size distribution profiles and in vitro release patterns were investigated by dynamic light scattering method and spectrophotometry. The stability of PPE-loaded liposomes was affected by the surface charges of the liposomes. As compared to neutral and anionic liposomes, cationic liposome formulations showed significantly lower physical stability. At the test storage conditions of different temperatures and pHs, the mean sizes of cationic PPE-loaded liposomes substantially increased. In contrast, neutral and anionic liposomes did not reveal significant changes in mean sizes upon various storage conditions. The neutral and anionic liposomes showed no significant differences in the release profiles of PPE after storage at various temperatures and pHs. Our results indicate that anionic and neutral liposome compositions might be more suitable for the formulations of PPE providing the higher stability.