• Asian-Australasian Journal of Animal Sciences
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• 제30권10호
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• pp.1416-1424
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• 2017

Objective: The objective of this study was to examine the effects of alfalfa flavonoids on the production performance, immunity, and ruminal fermentation of dairy cows. Methods: The experiments employed four primiparous Holstein cows fitted with ruminal cannulas, and used a $4{\times}4$ Latin square design. Cattle were fed total mixed ration supplemented with 0 (control group, Con), 20, 60, or 100 mg of alfalfa flavonoids extract (AFE) per kg of dairy cow body weight (BW). Results: The feed intake of the group receiving 60 mg/kg BW of AFE were significantly higher (p<0.05) than that of the group receiving 100 mg/kg BW. Milk yields and the fat, protein and lactose of milk were unaffected by AFE, while the total solids content of milk reduced (p = 0.05) linearly as AFE supplementation was increased. The somatic cell count of milk in group receiving 60 mg/kg BW of AFE was significantly lower (p<0.05) than that of the control group. Apparent total-tract digestibility of neutral detergent fiber and crude protein showed a tendency to increase (0.05<$p{\leq}0.10$) with ingestion of AFE. Methane dicarboxylic aldehyde concentration decreased (p = 0.03) linearly, whereas superoxide dismutase activity showed a tendency to increase (p = 0.10) quadratically, with increasing levels of AFE supplementation. The lymphocyte count and the proportion of lymphocytes decreased (p = 0.03) linearly, whereas the proportion of neutrophil granulocytes increased (p = 0.01) linearly with increasing levels of dietary AFE supplementation. The valeric acid/total volatile fatty acid (TVFA) ratio was increased (p = 0.01) linearly with increasing of the level of AFE supplementation, the other ruminal fermentation parameters were not affected by AFE supplementation. Relative levels of the rumen microbe Ruminococcus flavefaciens tended to decrease (p = 0.09) quadratically, whereas those of Butyrivibrio fibrisolvens showed a tendency to increase (p = 0.07) quadratically in response to AFE supplementation. Conclusion: The results of this study demonstrate that AFE supplementation can alter composition of milk, and may also have an increase tendency of nutrient digestion by regulating populations of microbes in the rumen, improve antioxidant properties by increasing antioxidant enzyme activities, and affect immunity by altering the proportions of lymphocyte and neutrophil granulocytes in dairy cows. The addition of 60 mg/kg BW of AFE to the diet of dairy cows was shown to be beneficial in this study.

• BMB Reports
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• 제37권6호
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• pp.720-725
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• 2004

A number of signaling molecules contain small pleckstrin homology (PH) domains capable of binding phosphoinositides or proteins. Phospholipase C (PLC)-${\gamma}1$ has two putative PH domains, an $NH_2$-terminal (PH1) and a split PH domain ($nPH_2$ and $cPH_2$). We previously reported that the split PH domain of PLC-${\gamma}1$ binds to phosphatidylinositol 4-phosphate (PI(4)P) and phosphatidylinositol 4,5-bisphosphate (PI(4,5)$P_2$) (Chang et al., 2002). To identify the amino acid residues responsible for binding with PI(4)P and PI(4,5)$P_2$, we used site-directed mutagenesis to replace each amino acid in the variable loop-1 (VL-1) region of the PLC-${\gamma}1$ $nPH_2$ domain with alanine (a neutral amino acid). The phosphoinositide-binding affinity of these mutant molecules was analyzed by Dot-blot assay followed by ECL detection. We found that two PLC-${\gamma}1$ nPH2 domain mutants, P500A and H503A, showed reduced affinities for phosphoinositide binding. Furthermore, these mutant PLC-${\gamma}1$ molecules showed reduced PI(4,5)$P_2$ hydrolysis. Using green fluorescent protein (GFP) fusion protein system, we showed that both $PH_1$ and $nPH_2$ domains are responsible for membrane-targeted translocation of PLC-${\gamma}1$ upon serum stimulation. Together, our data reveal that the amino acid residues $Pro^{500}$ and $His^{503}$ are critical for binding of PLC-${\gamma}1$ to one of its substrates, PI(4,5)$P_2$ in the membrane.

• Parasites, Hosts and Diseases
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• 제31권2호
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• pp.117-128
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• 1993

이 연구는 환자로부터 분리하여 무균 배양된 10개의 질트리코모나스 분리주에 대하여 병원성 여부를 판정하고 단백분해효소 관련 여부를 알아보고자 시도된 것이다. 질트리코모나스 분리주들은 마우스 피내 접종 실험을 통한 병원성 판정에서 약병원성 주, 중등도 병원성 주 및 강 병원성 주 등 3개 그룹으로 나눌 수 있었으며 중성 단백분해효소 및 산성 단백분해효소 활성도는 질트리코모나스 추출물 및 그 배양액에서 약 병원성 주에 비해 강 병원성 주의 활성도가 높게 나타나 피하농양 크기에 따른 병원성과 상기 단백분해효소의 비활성도(specific activi쇼) 사이에 상관관계가 있었음을 알 수 있었다(p < 0.05) 질트리코모나스 단백분해효소는 gelatin을 기질로 하는 SDS-PAGE 전기영동에서 RF치를 달리하는 5가지 분획대가 나타났으며 그 분획양상은 각 분리주 의 병원성에 따라 일정한 양상을 나타내었다. 그리고 여러가지 단백분해효소 억제제를 전기 영동 효소액에 처리했을 경우 antlpaln과 leupeptin 처리군에서는 분획이 전혀 나타나지 않았으며 EUTA 처리군에서는 대조군에 비해 그 활성이 약화된 분획이 관찰되었고, PMSF 처리군에서의 분획들은 대조군과 그 활성의 차이를 볼 수 없어 이들 단백 분해효소는 cystelne 단백분해효소로 추정되었다. 조직 세포에 대한 질트리코모나스 추출물의 세포독성은 병원성에 따라 차이가 있었고 추출물의 단백질 농도 $12.0{\;}\mu\textrm{g}/100{\mu}\ell$ 이상에서 세포 독성에 따른 병원성 구분이 용이하였다. 그리고 질트리코모나스 추출물에 단백분해효소 억제제를 처리한 결과 대조군에 비하여 세포 독성이 낮게 나타났으며, 특히 antipain 처리군에서는 조직 세포에 대한 세포 독성이 현저하게 낮았다. 이상의 결과로 보아 cysteine계로 추정되는 질트리코모나스의 단백분해효소는 특이한 전기영동 활성 분획상을 나타내었는 바 이들은 모두 충체의 병원성 및 세포 독성과 밀접한 관련이 있었다.

• 대한수의학회지
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• 제36권3호
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• pp.665-680
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• 1996

Bovine Pneumonic Pasteurellosis는 수송열(輸送熱)로 일반적으로 알려져 있는 질병으로서, 여러가지 요인의 복합적(複合的)인 작용에 의해 발병하는 것으로 알려져 있으나, Pasteurella haemolytica A1이 가장 주요(主要)한 인자(因子)로 밝혀져 있다. P haemolytica A1은 leukotoxin(LKT), lipopolysaccharide(LPS), capsular polysaccharide 등 여러가지의 병원성인자(病原性因子)을 생성한다. 이들 인자중 LKT가 가장 중요한 병원성인자로 밝혀져 있다. 이에 본 실험은 P haemolytical A1의 LKT 유전자를 Bacillus subtilis에서 발현(發現)시킴으로서 LPS에 오염(汚染)되지 않은 LKT을 대량으로 생산할 목적으로 실시되었다. 실험의 첫 단계(段階)로서 pLKT52 plasmid을 Sau3 A1의 제한효소을 이용하여 부분소화(部分消化)시킨 후 이 부분 소화(消化)된 유전자들로부터 3~5kb 크기의 유전자들을 순수분리하여 pUC18와 결합시킨 후 E coli NM522에 형질전환(形質轉換)시켰다. 이때 형질전환된 균주들은 LKT에 대한 단크론 항체인 MAb601을 이용하여 colony blot 법에 의해서 LKT 유전자 보유 및 발현여부(發現與否)을 조사하였다. 이들 양성 clone들은 제한효소분석(制限酵素分析), 염기서열분석(鹽基序列分析) 및 Western blot 등에 의해서 재확인(再確認)하였다. 총 9개의 양성 clone중 위의 방법에 의해서 한 clone을 선택(選擇)하여 lktCA insert를 재분리하여 shuttle vector에 subcloning 하였다. Subcloning된 LKT 유전자들은 shuttle vector의 종류(種類)(pHPS9, p602/20, pHPS9-Sac)와 각기(各其) 다른 종류(種類)의 B subtilis(spoO12A, BR121, WB3O, Raj1105) 숙주내(宿主內)에서 발현정도를 Western blot 법에 의해서 비교(比較)하였다. 이때 최적발현조건(最適發現條件)은 p602/20와 pBL1의 dual plasmid system을 이용하여 B subtilis spoO12A에서 2시간동안 IPTG로 발현을 유도(誘導)하는 것이었다. B subtilis에서 발현된 LKT을 visual 법과 neutral red uptake 법을 이용하여 소 폐포(肺胞) 대식구(大食求)에 대한 biological activity를 확인하였다. 발현된 LKT에 대한 LPS 오염은 LKT을 SDS-PAGE 후 silver stain에 의해서 확인하였다. 본 실험을 통해서 볼 때에 lktCA 유전자를 보유(保有)하고 있는 p602/20는 B subtilis에서 매우 불안정(不安定)하였고, 발현된 LKT는 세균자체(細菌自體)에서 생성되는 protease들에 의해서 파괴(破壞)됨으로서 농도(濃度)가 매우 낮았다. 이러한 문제점들은 다음 단계(段階)의 실험에서 해결되어야할 문제들이다.

• 한국초지조사료학회지
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• 제31권1호
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• pp.9-14
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• 2011

본 시험은 이탈리안 라이그라스 극조생종 품종을 육성하기 위하여 2001년부터 2010년까지 국립축산과학원 초지사료과에서 수행되었다. 이탈리안 라이그라스 신품종 '그린팜'는 2배체 품종으로서 엽은 녹색이며, 월동 전 초형은 직립형이고, 봄의 초형은 반직립형이다. 또한 '그린팜'는 4월 28일경에 출수하는 극조생종 품종으로 '그린팜'은 'Florida 80' 보다 지엽폭이 0.4mm 넓고, 지엽의 길이가 0.8 cm 길었으며, 출수기에 초장은 93 cm로 'Florida 80' 보다 5 cm 정도 작았다. 줄기의 두께는 'Florida 80' 보다 0.6 mm 굵고 이삭길이는 0.3 cm 짧았으며, '그린팜'의 내한성은 'Florida 80' 보다 다소 강한 품종이었다. '그린팜'의 건물수량은 11,790 kg/ha로서 'Florida 80'과 대등하였다. '그린팜'의 사료가치는 in vitro 소화율이 68.7%, 가소화양분총량(TDN)이 63.3%로서 'Florida 80' 보다 각각 1.9, 1.7% 높았고, 산성세제불용섬유(ADF)와 중성세제불용섬유(NDF)는 32.5 및 54.6%로 'Florida 80' 보다 각각 2.2 및 4.3% 낮았다.

• 패션비즈니스
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• 제5권4호
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• pp.158-175
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• 2001

The purpose of this study was to understand and improve the clothing habits and the apparel industry of North Korea in preparation for the reunification of South and North Korea. For this study, literary data, reports, periodicals, interviews and internet data of the two Koreas were reviewed. North Korean clothing habits used to be monotonous and uniform but nowadays people's clothes have become somewhat brighter in color and more diverse in design than before. In particular, liberal and individual dressing habits appeared among the privileged classes. When taking part in national events, women have to wear the traditional Korean costume, Hanbok, while men wear business suits for formal wear. In general, men don't wear Hanbok. Students have to be in uniforms but blue jeans, T-shirts with English logos were popular among them reflecting their sensitivity and openness towards western cultures. The brides usually wear pink Hanboks and the bridegrooms wear black business suits for their wedding. North Koreans also wear Hanbok on national holidays like South Koreans. Clothing is the most important item in the trade of process commission between North and South Korea. Trading items are mid to low end men's clothing for the most part due to less emphasis on fashion in the North. The processing is indirect trade and composed of sample making and contracting, sending out materials and production, carrying in goods and setting accounts. To activate South-North trade, establishment of infrastructure, stabilization of shipping, reducing high costs of distribution, building direct communication system by setting up office in a neutral zone and simplifying procedures in applying for the South and North Korea Economic Cooperation Fund. On the other hand, clothing and textiles education is carried on at art colleges, light industries colleges and commercial colleges in Pyongyang. Clothing institutes which study Hanbok and Western clothes, are installed in each city and province. Graduates who majored in clothing and textiles are posted in institutes or apparel factories. Their job is designing, patternmaking and sewing for their customers. Most of them are women and in good state of economic conditions. The North Korean clothing industry has been the core national industry that has developed based on overseas demand form the mid 1980s. The standard is that of South Korea in the early 1980s. In 1999, trade of North Korean textile products with trade counterparts such as Japan and China was $1.3 million in exports and $1.27 in imports. Of this amount the export takes up 25.4% of the total exports in North Korea. However, fundamentally even in sectors that are irrelevant to politics such as the fashion clothing industry, trust between the South and North should be a prerequisite. Only through this can exchange between North and South and economic cooperation contribute towards the reunification.

• Clinics in Shoulder and Elbow
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• 제17권1호
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• pp.25-30
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• 2014

Background: Normal elbow joint kinematics has been widely studied in cadaver, whilst in vivo study, especially of the forearm, is rare. Our study analyses, in vivo, the kinematics of normal forearm and of malunited forearm using a three-dimensional computerized simulation system. Methods: We examined 8 patients with malunited Monteggia fracture and 4 controls with normal elbow joint. The ulna and radius were reconstructed from CT data placing the forearm in three different positions; full pronation, neutral, and full supination using computer bone models. We analyzed the axis of rotation 3-dimentionally based on the axes during forearm rotation from full pronation to full supination. Results: Axis of rotation of normal forearm was pitch line, with a mean range of 2 mm, from full pronation to full supination, connecting the radial head center proximally and ulnar fovea distally. In normal forearm, the mean range was 1.32 mm at the proximal radioulnar joint and 1.51 mm at the distal radioulnar joint. However in Monteggia fracture patients, this range changed to 7.65 mm at proximal and 4.99 mm at distal radoulnar joint. Conclusions: During forearm rotation, the axis of rotation was constant in normal elbow joint but unstable in malunited Monteggia fracture patients as seen with radial head instability. Therefore, consideration should be given not only to correcting deformity but also to restoring AOR by 3D kinematics analysis before surgical treatment of such fractures.

• 무역상무연구
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• 제29권
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• pp.39-61
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• 2006

The governing law in international commercial arbitration may be divided into governing arbitration law and governing substantive law. The former governs the parties' arbitration agreement and the conduct of any subsequent arbitration. But the later governs the parties' substantive rights and obligations, which means the law that governs contract formation and performance, and the law to be applied by the arbitrator to the merits of the dispute. The purpose of this paper is to examine how to determine the substantive governing law when there is express choice or implied choice between parties. Moreover this author checked any restrictions on party autonomy and also any possibilities to deviate from the governing law. In case of express choice the sources of the law or rules of law might be the national law of one of the parties, the neutral law, the general principles of law or lex mercatoria according to the arbitration law selected by the arbitral tribunal. Some arbitration laws or rules empower the arbitrator to decide the case ex aequo et bono or to act as amiable compositions. If the governing law could be determined expressly or impliedly by the parties, the arbitral tribunal would make a selection. In this case the criteria for selecting a governing law are not exactly same from country to country. But failing any indication by the parties as to governing law, the arbitral tribunal should apply the rules of law, the law or the law under the rule of conflict that the arbitrators consider applicable, according to the governing arbitration law. Among the connecting factors offered by the conflict rules, (which means the factors that the arbitrators consider applicable), some legal systems give precedence to the formation of the contract, other system to the place of performance of the contract, and others to the closest connection or centre of gravity. But the Rome Convention, which unified the conflict rules of the contracting states, gives precedence to the law of the domicile of the party which has to effect the performance which is characteristic of the contract. Finally this author suggested the Choice of Law Clause which covers governing substantive law and governing arbitration law at the same time. Thus the UNIDROIT Principles as well as any national law may be included as a governing law in international arbitration. So when we make sales or service contract, we should take into consideration of the UNIDROIT Principles as a governing law or a supplement to the governing law.

• 한국응용과학기술학회지
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• 제31권1호
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• pp.101-112
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• 2014

본 연구는 고콜레스테롤 식이를 섭취한 랫드에서 파리유충 에탄올추출물(Ethanolic extract of fly maggot, EM)의 경구투여가 혈액 지질감소에 미치는 영향을 조사하였다. Sprague-Dawley 수컷 랫드를 이용하여 4 처리구(EM 투여량; 대조군=0, 5.0, 7.0, 9.0 mg/100 g 체중)로 구분해서 6주 동안 진행하였다. EM 투여군은 대조군과 비교할 때 혈청 중성지방, 총콜레스테롤, LDL-C가 유의하게 낮았다(p<0.05). HMG-CoA reductase activity는 대조군과 비교할 때 EM 투여군에서 낮았으나 총스테롤, 중성스테롤 및 담즙산 배설량은 EM 투여군에서 유의하게 높았다(p<0.05). EM의 혈액 콜레스테롤 감소와 관련한 생물학적 작용기작을 규명하기 위해서 고콜레스테롤 식이를 섭취한 랫드에서 유도된 생체유전자 sterol response element binding proteins (SREBPs) 및 the peroxisome proliferator-activated receptors ($PPAR{\alpha}$) 발현을 측정하였다. EM은 고콜레스테롤 식이를 공급받은 랫드의 간에서 SREBP-$1{\alpha}$, SREBP-2 mRNA 발현을 억압함과 동시에 $PPAR{\alpha}$ mRNA 발현을 촉진시키는 것으로 나타났다(p<0.05). 본 연구의 결과는 파리유충 에탄올추출물이 고콜레스테롤 식이를 섭취한 랫드에서 지질대사와 관련한 생화학적 매개변수 및 유전자발현 조절을 통하여 혈액 콜레스테롤을 낮춘다는 새로운 사실을 발견하였다.

• 한국식품과학회지
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• 제34권5호
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• pp.879-884
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• 2002

감잎으로부터 항보체 활성물질을 분리정제하기 위해 감잎 (250 g)을 $100^{\circ}C$에서 3시간 동안 열수 추출하고 분자량 10 kDa membrane을 사용하여 농축한 후 ethanol 침전과 methanol 추출을 통해 조다당류(DKC)를 얻었다. 조다당류의 정제는 DEAE-Toyopearl 650C와 Bio-gel P60을 사용하여 실시하였다. 정제된 DKC-1c는 $1000\;{\mu}g/mL$의 농도에서 고전경로를 통해서는 85.4% 활성화시켰고 부경로에서는 65.1% 활성화시켰다. 정제 다당류 DKC-1c는 분자량은 66.6 kDa이고 정제도가 높은 중성다당류로써 주요 구성당은 glucose(29.0 mol.%), arabinose(24.3 mol.%), galactose(16.2 mol.%) 순으로 검출되었다. 면역전기영동을 통하여 확인한 결과 DKC-1c는 C3를 부경로에서도 C3a와 C3b로 활성화시키는 complement activator임이 확인되었다.