• Title/Summary/Keyword: Neurospora

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A Gene of Neurospora crassa that Encodes a Protein Containing TPR Motifs

  • Lee Bheong-Uk
    • Proceedings of the Microbiological Society of Korea Conference
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    • 2003.05a
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    • pp.51-54
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    • 2003
  • Analysis of the Neurospora crassa genome data reveals at least 14 proteins that contain tetratricopeptide repeat (TPR) motifs. One of them shows over $60\%$ homology with SSN6 of Saccharomyces cerevisiae, a global repressor that mediates repression of genes involved in various cellular processes. Sequence analysis of its cDNA shows that it encodes a putative 102kDa protein. Mutant strains generated by RIP (repeat induced point mutation) process show four distinctive patterns of vegetative growth at various rates. They are male-fertile, yet all female-sterile and produced little or no perithecium. These results indicate that this gene is pleiotropic and involved in several cellular processes of vegetative growth, conidiation and sexual cycle. It is designated rcm-1(regulation of conidiation and morphology).

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Affinity labeling of the Vacuolar Arginine Transporter in Neurospora crassa (Neurospora crassa의 액포에 존재하는 arginine transporter의 표지방법)

  • ;Weiss, R. L.
    • Korean Journal of Microbiology
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    • v.27 no.2
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    • pp.108-116
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    • 1989
  • Based on the specificty of recognition of the vacuolar arginine transporter, N-p-nitrobenzoxycarbonyl (NBZ)-L-arginyl diazomethane was synthesized and used as an affinity label specific for the arginine transporter. This arginyl derivative ingibited both ATP-dependent and independent L-arginine transport into vacuolar membrane vesicles. When vacuolar proteins were labeled with radioactive NBZ arginyl diazomethane, the binding was irreversible, detached by treatment with base and blocked by treatment with cysteinyl blocking groups suggesting cysteine as a labeling site.

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Energy Status of Neurospora crassa Mutant nap in Relation to Accumulation of Carotenoids

  • Belozersk, Tatyana A.;Potapova, Tatyana V.;Isakova, Elena P.;Shurubor, Eugene I.;Savel'eva, Ludmila V.;Zvyagilskaya, Renata A.
    • Journal of Microbiology
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    • v.41 no.1
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    • pp.41-45
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    • 2003
  • N crassa mutant strain nap showed reduced growth rate, decreased electric membrane potential, and elevated intracellular ATP content in comparison to the wild type. Blue light induced a hyperpolarization of the membrane potential in both strains. The analysis of oxidative and phosphorylation activities of mitochondria isolated from the two strains has revealed that nap utilized more efficient oxidative pathways. The higher intracellular ATP content in the nap was presumably due to impaired transport systems of the plasma membrane, and to a lesser extent to the functioning of the fully competent respiratory chain. The excess ATP possibly accounts for carotenoid accumulation in the mutant.

Neurospora의 생육시기에 따른 호흡능의 변화와 자외선 감수성과의 상관관계

  • 이영녹
    • Journal of Plant Biology
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    • v.6 no.4
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    • pp.1-4
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    • 1963
  • Using conidia of Neurospora, changes in respiratory activities and the sensitivity to the ultraviolet light of the cells at different growing stages were measured by manometric methods, and the correlation between them was observed. Efficiency in the utilization of various carbon sources, such as, glucose, sucrose, maltose, starch and sodium acetate, in growth and exogenous respiration of N. crassa was also determined. Growth rate of N. crassa was decreased considerably in the medium containing sodium acetate than in the glucose medium and was almost zero in the lactose medium, whereas the utilization of sucrose, maltose and starch was ve교 high, as that of glucose. Respiratory activities of the cells veried considerably depending upon their different growing stages. Actively growing hyphae exhibited the greatest activity in exogenous glucose respiration, followed by germinating and activated conidia in decreasing order. There was no proportional relationship between the dose of ultraviolet light irradiated and its effect on the respiratory activity of the cells, though the more the dose of ultraviolet light, the more the injury. The sensitivity of the cells to ultraviolet light varied with the different respiratory activities of the cells linked to the developmental stages. In general, the more actively growing cells having high respiratory activities exhibited the more serious injury.

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Five Unrecorded Endophytic Fungal Species in Sordariomycetes from Korea (Sordariomycetes에 속하는 5종의 미기록 내생균)

  • Jae-Eui Cha;Eun-Ju Kim;Yun-Jeong Kim;Ahn-Heum Eom
    • The Korean Journal of Mycology
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    • v.51 no.4
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    • pp.335-347
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    • 2023
  • Sordariomycetes is the second largest class of Ascomycota, distributed throughout various habitat including terrestrial and aquatic environments and also existing as endophytes. We isolated endophytic fungal strains in Korea, identifying them based on their morphological characteristics and molecular analyses, using eight specific DNA regions for accurate genus identification. We identified five previously unrecorded endophytic fungal species in Korea: Chaetomium subaffine, Colletotrichum jiangxiense, Colletotrichum sydowii, Diaporthe vacuae and Neurospora tetraspora. In this study, we describe the morphological characteristics and present our phylogenetic analyses of these five fungal species.

EARLY EVENTS OCCURRING DURING LIGHT SIGNAL TRANSDUCTION IN PLANTS AND FUNGI

  • Hasunuma, Kohji;Ogura, Yasunobu;Yabe, Naoto
    • Journal of Photoscience
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    • v.5 no.2
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    • pp.73-81
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    • 1998
  • Light signals constitute major factors in regulating gene expression and morphogenesis in plants and fungi. Phytochrome A and B were well characterized red and far-red light receptors in plants. Red light signals increased the phosphorylation of 18 kDa protein, which was identified to be nucleoside diphosphate (NDP) kinase. The NDP kinase catalyzed autophosphorylation and had a protein kinase activity similar to MAP (mitogen activated protein) kinase. As candidates for blue light photoreceptors, cDNAs for CRY1 and CRY2 were isolated. The N-teminal regions of these proteins showed a high hornology to DNA photolyase. The 120 kDa protein first detected in Pisurn sativurn, which showed blue light induced phosphorylation was also detected in Arabidopsis thaliana. The 120 kDa protein was encoded by the nphl gene, which regulated positive phototropism of the plant. In Neurospora crassa, blue light irradiation of the membrane fraction prepared from roycelia stimulated the phosphorylation of the 15 kDa protein, which was also identifmd to be an NDP kinase. Recent progress in understanding early events in light signal transduction mainly in Pisum sativum Alaska, Arabidopsis thaliana and Neurospora crassa was summarized.

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