• IMMUNE NETWORK
• /
• 제5권4호
• /
• pp.247-251
• /
• 2005

Background: Natural killer (NK) cells are CD3 (-) CD14 (-) CD56 (+) lymphocytes. They play an important role in the body's innate immune response. They can induce spontaneous killing of cancer cells or virus-infected cells via the Fas/Fas ligand or the granzyme/perforin systems. The corticotropin-releasing hormone (CRH) is an important regulator for the body's stress response. It promotes proliferation and migration of various cancer cells through the CRH type 1 receptor under stress, and also inhibits NK or T cell activity. However, the relationship of CRH and NK cell migration to the target has not been confirmed. Herein, we study the effect of CRH on NK cell migration. Methods: We used the human NK cell line, NK-92MI, and tested the expression of CRH receptor type 1 on NK-92MI by RT-PCR. This was to examine the effect of CRH on tumor and NK cell migration, thus NK cells (NK-92MI) were incubated with or without CRH and then each CRH treated cell's migration ability compared to that of the CRH untreated group. Results: We confirmed that CRH receptor type 1 is expressed in NK-92MI. CRH can decrease NK cell migration in a time-/dose-dependent manner. Conclusion: These data suggest CRH can inhibit NK cell migration to target cells.

• 한국주거학회논문집
• /
• 제17권5호
• /
• pp.117-126
• /
• 2006

Although we have seen recently increasing number of eco-villages, any eco-village is not registered in Global Eco-village Network. It is important to review the definition of eco-villages and to provide useful basic information for future eco-villages. The main research aim is to analyze landscape characteristics of community garden and common spaces and to identify ecological symbolism of landscape created by residents in eco-villages. Seven eco-villages in Germany, Denmark and UK were selected and the following aspects were investigated through field survey; the settlement background, social and economical aspect, architectural specifications, recycled energy, and landscape characteristics of external spaces in eco-villages. The result indicated that the landscape of eco-village did intend neither to return to primitive society nor to control nature by technology. It intended to coexistence with nature. Primitive nature is symbolically restored and people who have it in mind strongly intend to grow together this common sense. Landscape creation has important role in creating common relationship between human living and nature. When we regard it conceptually as the eco-symbolism in landscape design of community garden, it presented the meaningful relationship with landscape and human life. It is not expression of landscape materiality and designed form but sense of place and landscape image. Landscape design would be expected to reveal the life stage and cycle from designed physical form and materiality through as time goes by when it is part of environmental circulation process by means of human living as meaningful relationship.

• 대한가정학회지
• /
• 제35권5호
• /
• pp.73-102
• /
• 1997

The purpose of this study was to identify the specific and empirical contents in terms of first-married stepmother about her stress and efforts to adapt to her stepfamily. This studies relied on the qualitative research method in order to approach stepmother's subjective experience. There were 6 stepmother respondents who were 6 first-marrieds living in Seoul or Kyoungki area. The data was collected by in-depth personal interview.‘Open coding process’, a part of grounded theory procedures were used to analyzed the data. The major findings can be summarized as follows: 1. When focused o stressors, the stress of stepmothers were categorized into 4 categories. Those were the following: 1 When focused on stressors, the stress of stepmothers were categorized into 4 categories. Those were the following: (1) Stepmother's own problem such as stepmother' identity problem; (2) Problems within stepfamily such as problems about relations with husbands, stepchildren, and her own children; (3) Problems of stepmother family's kin such as relational problems with former spouses, in-laws, and her parent; (4) Problems of social relationship such as isolation and alienation in social relationship and neighbors' prejudice about stepmother. 2. In overcoming stress from stepfamily life and adapting to it, 4 categories were identified as important. Contents of 4 categories were such like this: (1) Stepmother's own efforts - (a) give a positive meaning to marriage or remarriage (b) flexible personality (c) situation-accepting attitude (d) exclusive management of household income (e) job (f) perseverance for time to adaptation; (2) Positive feedback within stepfamily - (a) supportive husband (b) sense of unity as a couple (c) psychological reward from stepchildren (d) existence of stepmother's own child (e) behavior for family solidarity (f) family-centered religious life; (3) Stepfamily's relationship with former spouses such as breakdown in relationship with ex-wives or ex-husbands; (4) Support from stepmothers' kin and people in social network with stepmothers. It was concluded that to reduce stress and to help adapt to stepfamily, stepmother should make an effort, and that her stepfamily and her kin should cooperate and support. In addition, negative stereotype of stepmother and stepfamily should disappeared. It was suggested that studies on stepfamily should be vitalized and that programs for preparing remarriage and counseling for stepfamily should be spread.

• Journal of Applied Biological Chemistry
• /
• 제63권2호
• /
• pp.169-174
• /
• 2020

The objective of this study was to investigate the effects of a hypodermic injectable solution comprised of an LPM LB meso solution containing Melissa officinalis L. leaf extract (LPM) on the lipogenesis in the 3T3-L1 cells line. The lipid accumulation measured by oil red o staining in the 3T3-L1 adipocytes treated with LPM, which was reduced in a dose dependent manner and showed 91.7 to 62.9% compared to control group. Its effectiveness with a 50% solution was significantly higher than the hydroxycitric acid (positive control) treatment without showing cell cytotoxicity. In a quantitative real-time PCR, it was demonstrated that the LPM treatment appeared to upregulate the mRNA expression of the adipogenesis-related genes, which included the peroxisome proliferator-activated receptor gamma (50% concentration) while down-regulating the CCAAT-enhancer binding protein alpha (50% concentration) and the sterol regulatory element-binding protein 1c (10, 25, and 50% concentrations). The results from the current study suggest that the LPM could be useful biomaterials that can inhibit obesity in the 3T3-L1 cells, which could possibly be by regulating the specific adipogenic gene transcription factors.

• Journal of Applied Biological Chemistry
• /
• 제59권4호
• /
• pp.299-304
• /
• 2016

PCR 산물을 이용한 시퀀싱방법 중 Illumina 플랫폼으로 시퀀싱을 수행하면 100개 이상의 인위적인 시퀀스가 생겨나며, 그러한 인위적으로 형성되는 시퀀스에 의해 Operational taxonomic units를 기반으로 한 미생물생태 변화 및 네트워크 분석에 영향을 미친다. 이러한 문제점이 있음에도 불구하고 분변미생물생태를 분석하는데 Illumina에서 제공하고 있는 시퀀싱을 주된 방법으로 사용하고 있으며, 또한 그러한 시퀀스 기반의 분변미생물 생태분석 결과는 분변샘플상태(i.e., 분변 보관 기간, 분변양, 분변의 신선도)에 따라 상이하게 나타난다. 본 연구에서는 분변샘플의 동결건조가 시퀀스 데이터의 퀄리티를 향상시키는지 관해 조사하였으며, 이를 통해 분변샘플에 동결건조처리는 전체적인 미생물생태구조를 변화시키지는 않지만 인위적으로 형성되었을 가능성이 있는 시퀀스의 수를 감소시키는 것으로 확인되었다. 따라서, 분변으로부터 DNA를 추출하기 이전에 동결건조처리하는 방법을 Illumina 기반의 분변미생물생태분석에 사용하는 것을 권장한다.

• 한국실내디자인학회논문집
• /
• 제29호
• /
• pp.281-288
• /
• 2001

It shows 21st century as a networking society that is moving up the concept of global town and being industrialized with regional and personal connections. Individual's original idea and variety of 21th century may be respected by such environmental change, and age (Literary, human axis, technology axis, nature axis, kaining farming axis are central keyword) of culture that cultural capacity of individual becomes important fetters that create added value is forecasted to become. The moaning for space that is unemployment enemy by change of conceptional environment about cultural life and agreeable quality of life by development of Information-Communication technology may be required newly. As it does interaction along with development of science technique and Information-Communication technology, the future environment of various that complex! Is predicted to form network environment (existence space, cyberspace) newly. Human central interest is risen in technology balance hereupon, and these characteristic escaped in physical system that do with functional special quality and require human central and sensitive interaction. First, if examine about phenomenon by Digitize and chance aspect that is risen in 21th century, Digitize is time that action occurs fusion Tuesday that is various and Blur phenomenon of city·space, and the period to collapse the border between several individual. Second, importance more than man-centered and sensitive aspect of functional physical system is risen by digital age with development of technology medium, and as the five digital senses showed up, it suggests a sensual of the times and therefore the interest and direction get set up for the sensual sides of consumers. Third, special quality is, medium enemy by that digital space connotes meaning disk floret, variability, transparency, space red of extensity etc.. to burn and is digitalised, can be risen by symbolic, original individualities and emotional communication's the importance is required sensitivity enemy who is sympathy horn by fusion anger of individual. Technology of new media may open direction of new communication through interface that did not enjoy so far is going to supply new means that can express own to human

• 한국화재소방학회논문지
• /
• 제22권3호
• /
• pp.278-286
• /
• 2008

최근 대규모 및 초고층 복합 건물 화재에서 실시간 현장상황 파악 및 신속한 인명 구조와 구조대원의 안전한 생명 보장을 위하여 유비쿼터스 기반 첨단 방재시스템의 필요성이 증대되었다. 본 논문에서 초고층 건물 화재의 특성과 위험성을 분석하고 단순성과 확장성을 제공하는 효율적인 메쉬 라우팅을 이용한 피난유도 알고리즘을 제안하였다. 우리의 연구는 센서네트워크를 구축하여 초고층 건물의 화재 및 재난 발생시 실시간으로 화재 및 생존자 위치정보와 화재상황 정보를 감지하고 유도등과 연계한 메쉬 라우팅 알고리즘 기법을 이용하여 신속하고 안전한 피난경로를 제시한다.

• Journal of Information Processing Systems
• /
• 제12권2호
• /
• pp.263-274
• /
• 2016

The exceptional development of electronic device technology, the miniaturization of mobile devices, and the development of telecommunication technology has made it possible to monitor human biometric data anywhere and anytime by using different types of wearable or embedded sensors. In daily life, mobile devices can collect wireless body area network (WBAN) data, and the co-collected location data is also important for disease analysis. In order to efficiently analyze WBAN data, including location information and support medical analysis services, we propose a geohash-based spatial index method for a location-aware WBAN data monitoring system on the NoSQL database system, which uses an R-tree-based global tree to organize the real-time location data of a patient and a B-tree-based local tree to manage historical data. This type of spatial index method is a support cloud-based location-aware WBAN data monitoring system. In order to evaluate the proposed method, we built a system that can support a JavaScript Object Notation (JSON) and Binary JSON (BSON) document data on mobile gateway devices. The proposed spatial index method can efficiently process location-based queries for medical signal monitoring. In order to evaluate our index method, we simulated a small system on MongoDB with our proposed index method, which is a document-based NoSQL database system, and evaluated its performance.

• International Journal of Fuzzy Logic and Intelligent Systems
• /
• 제16권4호
• /
• pp.225-237
• /
• 2016

In conventional transportation problem (TP), all the parameters are always certain. But, many of the real life situations in industry or organization, the parameters (supply, demand and cost) of the TP are not precise which are imprecise in nature in different factors like the market condition, variations in rates of diesel, traffic jams, weather in hilly areas, capacity of men and machine, long power cut, labourer's over time work, unexpected failures in machine, seasonal changes and many more. To counter these problems, depending on the nature of the parameters, the TP is classified into two categories namely type-2 and type-4 fuzzy transportation problems (FTPs) under uncertain environment and formulates the problem and utilizes the trapezoidal fuzzy number (TrFN) to solve the TP. The existing ranking procedure of Liou and Wang (1992) is used to transform the type-2 and type-4 FTPs into a crisp one so that the conventional method may be applied to solve the TP. Moreover, the solution procedure differs from TP to type-2 and type-4 FTPs in allocation step only. Therefore a simple and efficient method denoted by PSK (P. Senthil Kumar) method is proposed to obtain an optimal solution in terms of TrFNs. From this fuzzy solution, the decision maker (DM) can decide the level of acceptance for the transportation cost or profit. Thus, the major applications of fuzzy set theory are widely used in areas such as inventory control, communication network, aggregate planning, employment scheduling, and personnel assignment and so on.

• Journal of Ecology and Environment
• /
• 제34권2호
• /
• pp.137-147
• /
• 2011

In 2009, United Nations Educational, Scientific and Cultural Organization (UNESCO) recognized the unique outstanding ecosystem biodiversity and distinct ecocultural values of the Shinan Dadohae Biosphere Reserve in the island region. The Dadohae area, which has been sustainably conserved for scores of years, boasts not only a unique ecosystem, but also has residents with a wide range of traditional ecological knowledge. In terms of understanding the soundness of the ecosystem network known as the landscape system, the recent expansion of environmental development has served to heighten the degree of consideration given not only to biodiversity, which has long been used as an indicator to assess ecosystem soundness, but also to assess cultural diversity. Man has used the surrounding landscape and living organisms as his life resources since the beginning. Moreover, whenever necessary, man has developed new species through cultivation. Biodiversity became a foundation that facilitated establishing cultural diversity such as food and housing. Such ecological knowledge has been conveyed not only to adjacent regions, but also at the international level. The recent rapid changes in the Dadohae area island ecosystem caused by the transformation of fishing grounds by such factors as climate change, excess human activities, and marine pollution, is an epoch event in environmental history that shows that the balance between man and nature has become skewed. Furthermore, this issue has moved beyond the biodiversity and landscape diversity level to become an issue that should be addressed at the cultural diversity level. To this end, the time has come to pay close attention to this issue.