• Journal of Ginseng Research
• /
• v.45 no.3
• /
• pp.390-400
• /
• 2021

Background: We recently showed that gintonin, an active ginseng ingredient, exhibits antibrain neurodegenerative disease effects including multiple target mechanisms such as antioxidative stress and antiinflammation via the lysophosphatidic acid (LPA) receptors. Amyotrophic lateral sclerosis (ALS) is a spinal disease characterized by neurodegenerative changes in motor neurons with subsequent skeletal muscle paralysis and death. However, pathophysiological mechanisms of ALS are still elusive, and therapeutic drugs have not yet been developed. We investigate the putative alleviating effects of gintonin in ALS. Methods: The G93A-SOD1 transgenic mouse ALS model was used. Gintonin (50 or 100 mg/kg/day, p.o.) administration started from week seven. We performed histological analyses, immunoblot assays, and behavioral tests. Results: Gintonin extended mouse survival and relieved motor dysfunctions. Histological analyses of spinal cords revealed that gintonin increased the survival of motor neurons, expression of brain-derived neurotrophic factors, choline acetyltransferase, NeuN, and Nissl bodies compared with the vehicle control. Gintonin attenuated elevated spinal NAD(P) quinone oxidoreductase 1 expression and decreased oxidative stress-related ferritin, ionized calcium-binding adapter molecule 1-immunoreactive microglia, S100β-immunoreactive astrocyte, and Olig2-immunoreactive oligodendrocytes compared with the control vehicle. Interestingly, we found that the spinal LPA1 receptor level was decreased, whereas gintonin treatment restored decreased LPA1 receptor expression levels in the G93A-SOD1 transgenic mouse, thereby attenuating neurological symptoms and histological deficits. Conclusion: Gintonin-mediated symptomatic improvements of ALS might be associated with the attenuations of neuronal loss and oxidative stress via the spinal LPA1 receptor regulations. The present results suggest that the spinal LPA1 receptor is engaged in ALS, and gintonin may be useful for relieving ALS symptoms.

• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.22 no.1
• /
• pp.345-353
• /
• 2021

This study examined the effects of social capital on psychological identity and practical wellbeing for the Korean baby boom generation. To achieve this, an empirical survey was carried out on baby boomers who use elderly welfare centers and cultural centers living in D City. The overall research results showed that trust (t=6.893, p<.05), participation (t=5.157, p<.05), network (t=8.093, p<.05), and norm and reciprocity (t=4.787, p<.05), as sub-factors of social capital for baby boomers, had a significant effect on their practical wellbeing. Psychological identity was moderated (t=2.023, p<.05) in the effect of trust on practical wellbeing, adopting the hypothesis. This means that the social ties and the strong trust relationship between family members and relatives, which built up amid rapid economic growth, work with positive expectations of social capital and have a major effect on practical wellbeing. Moreover, practical welling also rated high, along with the high trust relationship and psychological identity. Consequently, various exchange programs and group and volunteer activity programs for baby boomers should be established to decrease their psychological identity due to the loss of social roles. Moreover, the decline of activities at a time of retirement can slow practical wellbeing.

• Journal of Korean society of Dental Hygiene
• /
• v.21 no.4
• /
• pp.467-479
• /
• 2021

Objectives: The purpose of this study was to investigate the awareness of the performance and necessity of oral health promotion activities and seek ways to revitalize the professional role of dental hygienists in oral healthcare for the efficient oral care of the elderly. Methods: Eighty-five dental hygienists in charge of oral health promotion projects at public health centers and 38 dental hygienists in network dental clinical trials were investigated for their learning experience, performance experience, feasibility, and necessity of dental hygienists for general and oral health service items for the elderly. The collected data were analyzed using frequency analysis, chi-square test, and Mann-Whitney U test. Results: The degree of performance possibility according to the learning experience and performance experience of the dental hygienist for the whole body and oral health promotion activity items for the elderly showed that the degree of performance possibility was higher among those with experience compared to the non-experienced person, and it was statistically significantly higher (p<0.05). Conclusions: The dental hygienist's professional oral health service is a necessary system to improve practical knowledge and skills and to provide a wide range of professional oral health services for the elderly.

• Journal of the Korean Society for Library and Information Science
• /
• v.56 no.3
• /
• pp.27-46
• /
• 2022

Today, public libraries are contributing deeply to our society by strengthening their ability and services to identify and respond to users' needs through various programs. However, it is difficult to find a study that analyzed the research status of public library programs or changes over time. Therefore, for the purpose of systematically grasping research trends in the field of overseas public library programs, an intellectual structure analysis based on keyword profiling was performed. Specifically, subject terms analysis, network analysis and cluster analysis, and analysis by period/year were performed based on the controlled keywords (subject terms) of journal articles papers searched in the LISTA database. As a result, first, it was found that 9 subjects corresponding to all global/hot/local topics are leading the research in the field of overseas public library programs. Second, five research areas in the field of overseas public library programs(cultural programs, outreach programs, activity programs, public services, community) could be visualized and clearly identified. Third, research in the field of overseas public library programs began in earnest in the late 1990s and was active from the mid-2000s to the early 2010s, and after that, it was found to be somewhat stagnant until recently. This study is the result of specifically identifying research trends on programs that recently emerged as a major task of public libraries, and can be used as basic data and prior knowledge to explore the development direction of public library programs in the future.

• KIPS Transactions on Computer and Communication Systems
• /
• v.11 no.9
• /
• pp.305-316
• /
• 2022

With the flood of digital data owing to the Internet of Things and big data, cloud service providers that process and store vast amount of data from multiple users can apply duplicate data elimination technique for efficient data management. The user experience can be improved as the notion of edge computing paradigm is introduced as an extension of the cloud computing to improve problems such as network congestion to a central cloud server and reduced computational efficiency. However, the addition of a new edge device that is not entirely reliable in the edge computing may cause increase in the computational complexity for additional cryptographic operations to preserve data privacy in duplicate identification and elimination process. In this paper, we propose an efficiency-improved duplicate data elimination protocol while preserving data privacy with an optimized user-edge-cloud communication framework by utilizing a trusted execution environment. Direct sharing of secret information between the user and the central cloud server can minimize the computational complexity in edge devices and enables the use of efficient encryption algorithms at the side of cloud service providers. Users also improve the user experience by offloading data to edge devices, enabling duplicate elimination and independent activity. Through experiments, efficiency of the proposed scheme has been analyzed such as up to 78x improvements in computation during data outsourcing process compared to the previous study which does not exploit trusted execution environment in edge computing architecture.

• Journal of Internet Computing and Services
• /
• v.23 no.6
• /
• pp.39-48
• /
• 2022

Cyberattacks around the world continue to increase, and their damage extends beyond government facilities and affects civilians. These issues emphasized the importance of developing a system that can identify and detect cyber anomalies early. As above, in order to effectively identify cyber anomalies, several studies have been conducted to learn BGP (Border Gateway Protocol) data through a machine learning model and identify them as anomalies. However, BGP data is unbalanced data in which abnormal data is less than normal data. This causes the model to have a learning biased result, reducing the reliability of the result. In addition, there is a limit in that security personnel cannot recognize the cyber situation as a typical result of machine learning in an actual cyber situation. Therefore, in this paper, we investigate BGP (Border Gateway Protocol) that keeps network records around the world and solve the problem of unbalanced data by using SMOTE. After that, assuming a cyber range situation, an autoencoder classifies cyber anomalies and visualizes the classified data. By learning the pattern of normal data, the performance of classifying abnormal data with 92.4% accuracy was derived, and the auxiliary index also showed 90% performance, ensuring reliability of the results. In addition, it is expected to be able to effectively defend against cyber attacks because it is possible to effectively recognize the situation by visualizing the congested cyber space.

• Journal of Convergence for Information Technology
• /
• v.12 no.5
• /
• pp.237-245
• /
• 2022

This study examines the social discourse on the characteristics of the learning community in the untact era, and discusses the directions that learning communities for children could explore and consider in the pandemic situation and beyond. For this purpose, big data for one year, from January 20, 2020 to January 20, 2021, were collected through internet portal sites (includingincluding Google News, Daum, Naver and other News surfaces), using two keywords "untact" and "learning community", and analyzed by employing a word frequency and network analysis method. The analysis results show that several important terms, such as 'village education community', 'operation', 'activity', 'corona 19', 'support', and 'online' are closely related to the learning community in the untact era. The findings from this study also have implications for developing the learning community as an alternative model to fill the existing gaps in public care and education for children during the prolonged pandemic and afterwards. In conclusion, the study findings highlight that it is meaningful to identify key terms and concepts through word frequency analysis in order to examine social trends and issues related to the learning community.

• Korean Journal of Animal Reproduction
• /
• v.24 no.4
• /
• pp.375-383
• /
• 2000

The nuclear maturation and developmental competence of immature, oocytes collected from donors at various morphology of corpus luteum (CL) and fertilized in vitro was investigated by comparing the meiotic activity and the yields of embryos. Ovaries were divided and classified into 4 groups as the following criteria : Group 1 ; ovaries showed evidence of recent ovulation (corpus hemorragicum). Group 2 ; apex of CL was red or brown. Vasculization was limited to periphery of CL. Group 3 ; apex of CL was orange or tan. Vasculization was covered over apex of CL. Group 4 ; CL was light yellow to white and firm in texture and the vascular network on the surface of CL had disappeared. Modified TCM 199 was used for maturation in vitro of immature oocytes and development was induced by using TLP-PVA as a basic medium. When oocytes collected from each group of donors had been matured for 4, 14, and 24 hours in vitro, the proportion of oocytes reaching metaphase I and metaphase II were not different among oocytes from 4 group of ovaries. Mature metaphase II stage of oocytes in each group was first observed at 14 hours, whereas completion of maturation of. oocytes in each group was at 24 hours. Luteal morphology of ovaries had little effect on the proportion of embryos reached 2 cells and 8 cell stage. However, the proportion of embryos cleaved to morula and blastocyst stage was significantly higher in the oocytes obtained from group 1 and 3 than in the oocytes from group 2 and 4 (p＜0.05). This data suggest that reproductive status of the donor significantly influence the yield of in vitro embryos.

• Journal of Life Science
• /
• v.33 no.1
• /
• pp.34-42
• /
• 2023

In a signal transduction network, from the perception of stress signals to stress-responsive gene ex- pression, binding of various transcription factors to cis-acting elements in stress-responsive promoters coordinate the adaptation of plants to abiotic stresses. Among the AP2/ERF transcription factor family genes, group VII ERF genes, such as RAP2.12, RAP2.2, RAP2.3, AtERF73/HRE1, and AtERF71/ HRE2, are known to be involved in the response to hypoxia stress in Arabidopsis. In this study, we dissected the HRE1 promoter to identify hypoxia-responsive region(s). The 1,000 bp upstream promoter region of HRE1 showed increased promoter activity in Arabidopsis protoplasts and transgenic plants under hypoxia conditions. Analysis of the promoter deletion series of HRE1, including 1,000 bp, 800 bp, 600 bp, 400 bp, 200 bp, 100 bp, and 50 bp upstream promoter regions, using firefly luciferase and GUS as reporter genes indicated that the -200 to -100 region of the HRE1 promoter is responsible for the transcriptional activation of HRE1 in response to hypoxia. In addition, we identified two putative hypoxia-responsive cis-acting elements, the ERF-binding site and DOF-binding site, in the -200 to -100 region of the HRE1 promoter, suggesting that the expression of HRE1 might be regulated via the ERF transcription factor(s) and/or DOF transcription factor(s). Collectively, our results suggest that HRE1 contains hypoxia-responsive cis-acting elements in the -200 to -100 region of its promoter.

• Nutrition Research and Practice
• /
• v.17 no.4
• /
• pp.682-697
• /
• 2023

BACKGROUND/OBJECTIVES: Tibetan tea is a kind of dark tea, due to the inherent complexity of natural products, the chemical composition and beneficial effects of Tibetan tea are not fully understood. The objective of this study was to unravel the composition of Tibetan tea using knowledge-guided multilayer network (KGMN) techniques and explore its potential antioxidant and hypolipidemic mechanisms in mice. MATERIALS/METHODS: The C57BL/6J mice were continuously gavaged with Tibetan tea extract (T group), green tea extract (G group) and ddH2O (H group) for 15 days. The activity of total antioxidant capacity (T-AOC) and superoxide dismutase (SOD) in mice was detected. Transcriptome sequencing technology was used to investigate the molecular mechanisms underlying the antioxidant and lipid-lowering effects of Tibetan tea in mice. Furthermore, the expression levels of liver antioxidant and lipid metabolism related genes in various groups were detected by the real-time quantitative polymerase chain reaction (qPCR) method. RESULTS: The results showed that a total of 42 flavonoids are provisionally annotated in Tibetan tea using KGMN strategies. Tibetan tea significantly reduced body weight gain and increased T-AOC and SOD activities in mice compared with the H group. Based on the results of transcriptome and qPCR, it was confirmed that Tibetan tea could play a key role in antioxidant and lipid lowering by regulating oxidative stress and lipid metabolism related pathways such as insulin resistance, P53 signaling pathway, insulin signaling pathway, fatty acid elongation and fatty acid metabolism. CONCLUSIONS: This study was the first to use computational tools to deeply explore the composition of Tibetan tea and revealed its potential antioxidant and hypolipidemic mechanisms, and it provides new insights into the composition and bioactivity of Tibetan tea.