• Journal of The Korean Society of Clinical Toxicology
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• v.7 no.1
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• pp.19-22
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• 2009

Although Nerium indicum poisoning is a globally rare occurrence, Nerium oleander poisoning is known to occur frequently in the Mediterranean regions. To our knowledge, this is the first reported case of accidental Nerium indicum poisoning in Korea. Its poisoning symptoms and signs are similar to that of digitalis poisoning, because of the presence of cardiac glycosides in Nerium indicum. A 16-year-old boy was admitted to the emergency department four hours prior to the accidental ingestion of Nerium indicum petals. The patient complained of nausea, vomiting, and dizziness. His initial vital signs were stable; laboratory blood test results were within normal levels, except for the blood digoxin level (1.5 ng/dL). An electrocardiogram (ECG) analysis showed normal sinus rhythm, progressive PR prolongation and second-degree Morbiz type I AV block. Conservative treatments including activated charcoal administration were conducted, because toxic symptoms and signs were not severe. The patient was admitted to the intensive care unit for close observation. His ECG was converted to normal rhythm after 1 day and the toxic symptoms and signs were completely resolved after 4 days.

• Journal of Plant Biology
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• v.32 no.3
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• pp.163-172
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• 1989

Nerium indicum has adaxial colleters on each petiole, bract, bracteole, calyx and corolla. The colleters are finger-shaped sometimes lobed structures with tall heads on short stalks. The petiolar colleters are more abundant than those on the bract, bracteole, calyx and corolla but their structure is similar. Meristematic activity in a small group of protodermal and hypodermal cells at the base of the developing leaf gives rise to the colleter that further differentiates by frequent anticlinal and periclinal divisions. Many of the colleters are vascullarized, sometimes a layer of redially elongated cells is present beneath the epithelial cells. Senescence of a colleter begins when the leaves have either completed their growth or are still expanding. In senescent colleters, the central cell cytoplasm and nuclei are considerably reduced. Thin layer chromatography of the freshly harvested exudate of colleter shows the presence of rhamnose, glucose and arabinose sugars.

• Journal of Plant Biology
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• v.34 no.1
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• pp.1-8
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• 1991

A sequential sub-cellular study of the epicarp of Nerium indicum has been performed. Outer epidermis of the ovary wall is covered externally with a thin cuticle. Cytoplasm possesses most of the cell organelles in the ovary stage itself. Outermost zone of the pericarp is the epicarp, developing from the outer epidermis. In the developing fruit, cell organelles are found with its maximum intensity. In mature fruit, the epicarp becomes multilayered due to additional development of few collenchymatous cells close to the outermost layer. Epicarpic cell possesses large central vacuole, around which a thin layer of cytoplasm is present. Number of cell organelles are considerably reduced in the mature fruit. In the ovary stage starch grains are electron transparent, while in the mature fruit it is fruit it is electron transluscent.

• Proceedings of the Zoological Society Korea Conference
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• 2003.08a
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• pp.160.1-160
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• 2003

No Abstract, See Full Text

• Korean Journal of Environmental Agriculture
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• v.34 no.2
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• pp.128-133
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• 2015

BACKGROUND: Plant extracts have been used as environment friendly agricultural materials for organic farming in South Korea. However safety evaluation on the plant extracts was not properly tested. The aim of this study was to evaluate safety of the extracts from Melia azedarach, Nerium indicum and Coptis chinensis on cultivating rice. METHODS AND RESULTS: Pant extarcts 300-fold diluted were treated on rice, and residues of M. azedarach, N. indicum and C. chinensis were determined. The analytes from the rice samples were detected by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS). The method was validated, and good linearities ($r^2=0.995-0.998$), specificity, and recoveries were obtained. Limits of detection were 0.01 mg/kg for all of the target compounds. Recoveries were 79.3-118.3% at 0.1 mg/kg and 75.2-111.5% at 0.5 mg/kg. The residue levels were below 0.030 mg/kg for azadirachtin, 0.320 mg/kg for oleandrin and 1.460 mg/kg for berberine. CONCLUSION(S): The extracts of M. azedarach, N. indicum and C. chinensis contained azadirachtin, oleandrin and berberine as an active ingredient, respectively. The residue of three active ingredients dramatically decreased after treatment in all fruits, stems and roots of rice.

• Journal of Life Science
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• v.20 no.8
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• pp.1221-1229
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• 2010

Nerium indicum, an India-Pakistan-originated shrub belonging to the oleander family, is reported to possess many pharmacological activities including cardiac muscle stimulation, and anti-diabetes, anti-angiogenesis, anti-cancer and neuro-protective activities. However, the anti-inflammatory properties of N. indicum were unclear. In this study, we investigated the effects of ethanol extract of the N. indicum leaf and stem (ENIL and ENIS) on the expression of anti-inflammatory mediators in U937 human pre-monocytic cell models. In U937 cells stimulated with phorbol 12-myristate-13-acetate (PMA), pre-treatment with ENIS significantly inhibited the expression of both cyclooxygenase-2 (COX-2) mRNA and protein, which are associated with inhibition of the release of prostaglandin $E_2\;(PGE_2)$, whereas the inhibitory effects appeared weakly in ENIL. Moreover, ENIS significantly attenuated PMA-induced IkappaB ($I{\kappa}B$) degradation and suppressed elevated nuclear factor kappa B (NF-${\kappa}B$) nuclear translocation. Taken together, these findings provide important new insights that N. indicum exhibits anti-inflammatory properties by suppressing the transcription of pro-inflammatory cytokine genes through the NF-kB signaling pathway.