• Journal of Life Science
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• v.19 no.11
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• pp.1529-1537
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• 2009

To elucidate further the antitumor effects of a natural L-arginine analogue, L-canavanine, the mechanism underlying apoptogenic activity of L-canavanine and its modulation by protein tyrosine kinase $p56^{lck}$ was investigated in human Jurkat T cells. When the cells were treated with 1.25 to 2.5 mM L-canavanine for 36 h, several apoptotic events including mitochondrial membrane potential (${\Delta\Psi}m$) loss, activation of caspase-9, -3, -8, and -7, poly (ADP-ribose) polymerase (PARP) degradation, and DNA fragmentation were induced without alteration in the levels of Fas or FasL. These apoptotic changes were more significant in $p56^{lck}$-deficient Jurkat clone JCaM1.6 than in $p56^{lck}$-positive Jurkat clone E6.1. The L-canavanine-induced apoptosis observed in $p56^{lck}$-deficient JCaM1.6 cells was significantly reduced by introducing $p56^{lck}$ gene into JCaM1.6 cells by stable transfection. Treatment of JCaM1.6/lck cells with L-canavanine caused a transient 1.6-fold increase in the kinase activity of $p56^{lck}$. Both FADD-positive wild-type Jurkat T cell clone A3 and FADD-deficient Jurkat T cell clone I2.1 exhibited a similar susceptibility to the cytotoxicity of L-canavanine, excluding involvement of Fas/FasL system in triggering L-canavanine-induced apoptosis. The L-canavanine-induced apoptotic sub-$G_1$ peak and activation of caspase-3, -8, and -7 were abrogated by pan-caspase inhibitor (z-VAD-fmk), whereas L-canavanine-induced activation of caspase-9 was not affected. These results demonstrated that L-canavanine caused apoptosis of Jurkat T cells via the loss of ${\Delta\Psi}m$, and the activation of caspase-9, -3, -8, and -7, leading to PARP degradation, and that the $p56^{lck}$ kinase attenuated the ${\Delta\Psi}m$ loss and activation of caspases, and thus contributed as a negative regulator to L-canavanine-induced apoptosis.

• Korean Journal of Environmental Agriculture
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• v.11 no.3
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• pp.261-268
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• 1992

The membranes of mitochondria and chloroplasts contain a number of pigments that can act as endogenous sensitizers to produce activated oxygen species, most efficiently in blue light, which, in turn, attack functional targets in membranes. Therefore, intense blue light from the sun can exert various adverse effects on the functional and structural integrity of the membranes: one of the biochemical events of these negative effects could be the oxidative degradation of the unsaturated fatty acid constituents of membrane polar lipid. It may be assumed that as a strategy to avoid the light induced fatty acid degradation in membranes plant cells, responding to high intensity blue light, change the fatty acid compositions of membrane lipid in such that more-unsaturated fatty acid constituents are replaced by lessunsaturated fatty acid constituents. The results obtained in the present study, most importantly the measurements of double bond index of membrane polar lipid in concert with other measurements such as light quaility-dependent membrane peroxidation and the activities of membrane-bound proteins, seem to support this assumption.

• Korean Journal of Community Nutrition
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• v.19 no.5
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• pp.459-467
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• 2014

Objectives: The purpose of this study was to suggest the strategies for improvement of home-delivered meal services for the elderly, to identify reasons for recipients to get started with the services and to evaluate the attitude, acceptability and adaptation of recipients to the services from the perspective of life context. Methods: The data was collected through face-to-face in-depth interviews with eighteen low-income elderly recipients of home-delivered meals and analyzed using a qualitative research method. Results: The results were deduced as four themes which comprised of long-term vulnerable socioeconomic contexts resulted in entry to the services, conflicting acceptability to the services, passive adaptation to taking the services, and positive practices to cope with supplement free meals or other services. The service participation was initiated because of a combination of prolonged, vulnerable socioeconomic contexts, including poverty and unexpected life events such as diseases, disability, living alone, aging and unemployment. With regard to taking the services, conflicting acceptability was observed: positive aspects including saving living cost and good quality of meals, and negative aspects including lack of a tailored service and feeling of stigma. Although the recipients needed an individualized service, they did not express their needs and demands for the services and they accepted the unavailability as an accustomed, prolonged vulnerable socioeconomic context. With regard to lack of tailored services, either self-solution such as modification of eating patterns or community-based network and services were used. Conclusions: We suggest that a system to concretely identify recipients' attitude, acceptability and adaptation for home-delivered meal services should be developed in the establishment of a tailored nutrition support system for the low-income elderly.

• Molecules and Cells
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• v.26 no.4
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• pp.396-403
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• 2008

The first ORF of the ARV S1133 S1 segment encodes the nonstructural protein p10, which is responsible for the induction of cell syncytium formation. However, p10-dependent signaling during syncytium formation is fully unknown. Here, we show that dominant negative RhoA, Rho inhibitor C3 exoenzyme, ROCK/Rho-kinase inhibitor Y-27632 and Rac1 inhibitor NSC23766 inhibit p10-mediated cell fusion. p10 over-expression is concomitant with activation and membrane translocation of RhoA and Rac1, but not cdc42. RhoA and Rac1 downstream events, including JNK phosphorylation and transcription factor AP-1 and $NF-{\kappa}B$ activation, as well as MLC expression and phosphorylation are simultaneously activated by p10. p10 point mutant T13M possessed 20% fusion-inducing ability and four p10 fusion-deficient mutants V15M, V19M, C21S and L32A reduced or lost their ability to activate RhoA and Rac1 signaling. We conclude that p10-mediated syncytium formation proceeds by utilizing RhoA and Rac1-dependent signaling.

• Korean Institute of Interior Design Journal
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• v.19 no.2
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• pp.206-215
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• 2010

The negative memories which come from tragic events such as war, terror or various conflicts in modern cities have been threatening the mental health of city-dwellers. This thesis focuses on 'healing public space' that will help recover the life of city-dwellers who sustain various trauma. For this, the author investigated the significant memorial space through 1980-2010 from site visits, interviews, and related theses and books and then extracted necessary information from each period. This thesis aims to find the changes of the memorial concepts and visitor's reaction for last 30years. 1)1980-: Healing concept and visitor's positive response 2)1990-: Healing by emotional experience approaches to the memorial museum such as narrative approach, exhibition media using 5senses, interactive display and preserving trace, 3)early 2000-: Healing by architectural experience 4)2002-: present: Healing projects by various programs. Steady advances in memorial design for healing trauma are due to the efforts for emotional design approach to eeply move the visitors and the efforts have to be continued. The study emphasize the importance of attracting eople by experimental, emotional design contents not just by aesthetic and functional public design ideas.

• Journal of Korean Academy of Nursing
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• v.42 no.6
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• pp.907-915
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• 2012

Purpose: A diagnosis of breast cancer is one of the most traumatic events that threatens a woman's life, but while women adapt to and overcome these threats, they not only experience negative aspects, but also growth. The purpose of this study was to identify the many factors that affect growth, and to provide fundamental information for nursing interventions, which can help the women in their growth. Methods: The participants in this study were 131 married women patients with breast cancer, who were on medical treatment in one of two university hospitals, in Seoul and Chungnam. Data were collected for posttraumatic growth, self-esteem, cancer coping questionnaire, marital intimacy, and body image. The data were analyzed using the SPSS 19.0 program (IBM). Results: Interpersonal cancer coping, intrapersonal cancer coping (planning) and self-esteem accounted for 29.0% of posttraumatic growth. Conclusion: These findings indicate that in order to help the women's growth after the trauma of breast cancer, it is necessary to enhance their self-esteem, and to develop psycho-social nursing supportive programs.

• Korean Journal of Child Studies
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• v.19 no.2
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• pp.49-64
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• 1998

The theoretical process model was used to study problem behavior of children growing up at-risk. A sample of 339 children living in at-risk families were selected from among 2, 134 children in grades 4, 5, and 6 of five elementary schools in Seoul. The instruments were five risk factor scales and four protective factor scales. The Child Behavior Checklist was also used. The results of the hypothetical model of children at-risk showed that the factor of socioeconomic status led to positive child rearing attitudes in mothers, which is turn influenced children's problem behavior indirectly. In contrast, stressful life events and mother's depression influenced children's problem behavior directly. However, negative family functioning, marital discord and father's depression did not influence mother's child rearing attitudes or children's problem behavior. Mother's child rearing attitudes directly influenced children's social competence and academic achievement and indirectly influenced their problem behavior. Academic achievement did not influence problem behavior directly, but social competence influenced it directly. Mother's child rearing attitude was the most significant factor affecting children's adaptation.

• The Journal of the Convergence on Culture Technology
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• v.10 no.3
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• pp.379-390
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• 2024

We looked at the background of how Western dance (Dance Sports, Social Dance) was first introduced as social dance in the past in Korean society with Confucian culture. In addition, we look into the social aspects of dance at the time, such as the spread of social dancing before the Korean War, the path through which the social dancing craze developed in the 1950s after the Korean War, and the decadent trend immediately after the Korean War, and how it transformed into a negative cultural phenomenon. We reexamined the series of events along with their social background. And now, social dance and dance sports have undergone such a significant change in perception in Korean society that they have become an indispensable element of life, to the point where the sentence is no longer unfamiliar in our country. Dancesport is an artistic indoor sport that combines elements of dance and sport. We also studied what changes have occurred in Korean society, including modern artistic elements.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.19 no.6
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• pp.299-323
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• 2018

This study confirmed the life career branching points, and studied qualitatively the career strategy and career identity at that time. The participants in this study were three students from third graders of middle school, three ones from third graders of high school, and three before and after college graduation, which correspond to the vertical transition stage of school education. Three participants were selected before and after 30 years of age, three before and after 40 years of age, and three before and after 60 years of age. Subject analysis of the contents of the 18 interviews, showed that the life career branching point appeared in middle school grade 3, high school grade 3, until employment after graduation, within 3 years after entering their first job, early 40 years, 60 years old, and 80 years old. Second, external situations were due to the influence of important others, or external stimuli, environment, and career events. Third, negative emotions were repeated for each life career branching point. Fourth, as a result of the interview, the career identity confusion was repeated in every life career branching point. Fifth, the career strategy at the life career branching point was categorized as an approach strategy and avoidance strategy.

• Journal of The Korean Society of Integrative Medicine
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• v.7 no.4
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• pp.241-251
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• 2019

Purpose : The purpose of this study was to investigate the depression, self-efficacy, and stress level of middle-aged women and to identify the factors affecting on stress, and to provide base line data for the development of nursing intervention for stress reduction of middle-aged women. Methods : Participants were 200 middle-aged women aged 40 to 60 who live in Seoul and Gyeonggi Province. Data collection period was from March 1, 2019 to April 15. In this study, the questionnaire of life events-stress, depression scale, self-efficacy was used. Data were analyzed by average, standard deviation, minimum, maximum, correlation and regression using the SPSS Program 21.0. Results : The results of this study, the correlation between depression, self-efficacy and stress in middle-aged women, depression and self-efficacy (r=-.264, p<.001), self-efficacy and stress (r=-.279, p<.001) had a significant negative correlation, depression and stress (r=.268, p<.001) had a significant positive correlation. Depression and self-efficacy were the factors affecting the stress of middle-aged women. Depression was β =.207 (p<.001) and self-efficacy β =-.222 (p=.003). Depression had the greatest influence on stress, followed by self-efficacy. Conclusion : Middle-aged women are an important time to prepare for a successful old age. Considering that the current middle-aged women are the subjects of the future aging society, their physical, psychological, and social problems are very important in preventing the elderly problems of the aging society. Therefore, effective nursing interventions should be developed that to reduce the depression and stress in middle-aged women and to improve self-efficacy and improve the quality of life of middle-aged women.